• Preventive Nutrition and Food Science
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• 제2권3호
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• pp.259-262
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• 1997

The microstructures of soybean milk curds, prepared by different coagulation methods, were observed by the scaning electron microscope. Th curd coagulated by theaddition of bacerial soybean mil clotting enzyme showed little textural changes and gave smoother gel than those prepared either by lactic acid fermentation using Streptococcus thermophilus or by the addition of CaSO$_4$. The curds obtained by lactic acid fermentation and by the addition of inorganic salt exhibited three dimensional network structure which indicated harder gel than that prepared by soybean mil clotting enzyme.

• 한국식품영양학회지
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• 제9권3호
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• pp.265-270
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• 1996

When new told grape with high acidity Is used wine, their sensory scores are decreased. Using three available methods reducing excess acidify In winemaking, they were malo-lactic fermentation, addition of apple pomace and malo-alcohol fermentation. The chemical monponents and sensory evaluation of wine were determined. They were found in pink wine added apple pomace that tatal acidity and malic acid content were 0.76 and 0.484%. Color Intensity (A520+A420) in pink wanes, their valuse were in the range 8.21~9.42, while the value of red wine was 20.92. Browning index and total phenol contents in pink wine, their values were In the range of 1.36∼1.42 and 3,300∼3,800mg 11. The sensory evaluation showed that the pink wine added apple pomace(10%) was highest in taste and total scores (P<0.05), and the taste of wine has improved.

• 한국식품조리과학회지
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• 제24권6호
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• pp.829-836
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• 2008

This study was carried out to investigate the properties of mul-kimchi made with allium victorialis. Chemical and sensory characteristics were determined during fermentation at $4^{\circ}C$ for 4 weeks. The pH decreased with an increase in total acidity. Mul-kinchi showed salinity properties ranging within $1.0{\sim}1.5%$ during fermentation. Hunter's color L, a-values were gradually decreased, but b-value was increased. In the texture, hardness and strength decreased as the aging time increased. The numbers of total- and lactic bacteria cells rapidly increased up to the second week, and this slowly increased thereafter. Sensory evaluation of mul-kinchi was favored for appearance, flavor, taste, texture and overall acceptability during the second week. Sensory properties of well ripened odor and sour taste negatively correlated with physicochemical properties of pH and hardness. Sour odor and sour taste were positively correlated with acidity and total- and lactic acid bacteria.

• 동아시아식생활학회지
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• 제7권1호
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• pp.65-70
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• 1997

To investigate the food preservative effects of leaf mustard Dolsan(Brassica juncea), the pretreated extract of leaf mustard Dolsan were added to Kimchi and the results are as following. pH of Kimchi added leaf mustard Dolsan extracts were lower than that of control at initial stage, but after 6 days of fermentation, pH was higher in order of Kimchi added the pretreated extract of leaf mustard Dolsan (hydrolyzed at 3$0^{\circ}C$ for 24 hours, PEM), the unpretreated extract of leaf mustard Dolsan(UEM) and control. The total number of bacteria and lactic acid bacteria increased rapidly at the initial stage of fermentation and reached plateau at 2 days of fermentation. The number of bacteria and lactic acid bacteria of Kimchi added leaf mustard Dolsan extracts were lower than that of control ,a and antimicrobial activity of PEM in Kimchi was higher than that of UEM. After 2 days fermentation, sensery value of Kimchi added PEM was more excellent than that of control.

• 한국식품조리과학회지
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• 제12권3호
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• pp.300-304
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• 1996

증편반죽의 증자과정 중에 일어나는 변화양상을 규명하고자 발효시간을 0에서 10시간까지 달리한 증편제품들을 비교 검토하였다. 발효시간에 따른 증편제 품의 화학적 성질들의 변화양상은 반죽시료의 경우와 유사하였으나 증자과정 중 pH는 약간 증가한 반면에 적정산도, lactic acid, 총 당과 환원당, 가용성 단백질 과 총 유리아미노산은 감소하였고 특히 환원당이 크게 감소하였으며 또한 유리아미노산의 종류별로는 다소 증가 혹은 감소하여 일정한 경향을 보이지 않았다.

• Preventive Nutrition and Food Science
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• 제9권3호
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• pp.218-221
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• 2004

Irradiated red pepper powder (IRPP) was tested for its ability to retard fermentation and to maintain a high quality of Kimchi by the reduction of the initial microbial load. Kimchi containing IRPP at the doses of 0, 5, 10, 15, or 20 kGy was prepared. Quality indices for Kimchi in this study were pH, titratable acidity, reducing sugar content, total microbial count, lactic acid bacterial load, and sensory evaluation. Based on the pH and titratable acidity, the Kimchi with IRPP showed a retarded fermentation until 15 days. The number of the total aerobes and lactic acid bacteria of the Kimchi with IRPP were lower by about 1 log CFU/mL compared to control at day 0, however, the counts increased to 8.5 log CFU/mL after 10 days, which was similar to the control group. Kimchi that was fermented with 5 kGy IRPP was better than control and other treatments in odor and color, whereas the control scored highest in taste. Addition of IRPP showed a limited retardation of Kimchi fermentation without other quality deterioration.

• 한국식품저장유통학회지
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• 제9권3호
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• pp.292-297
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• 2002

매실 추출물의 식품 보존제로서의 이용가능성을 검토하기 위하여 각각 0.5%, 1% 농도의 매실 추출물의 김치 유산균의 성장에 미치는 영향을 조사하고 1%, 3%의 매실 추출물을 김치에 첨가하여 숙성에 따른 이화학적, 미생물학적 변화를 관찰하였다. 매실 추출물의 농도가 높을수록 유산균의 성장을 억제하는 것으로 나타났으며 김치 숙성 중 pH, 산도, 총균수, 유산균수, 색상, 기호성을 대조구와 비교한 결과 매실 추출물 첨가구의 경우 pH 감소속도는 완만하였으며 산도 증가도 낮게 나타났다. 매실 첨가에 의한 김치 숙성 중 유산균수의 변화는 대조구보다 낮은 경향을 보였으나 숙성 25일째 유산균수가 6 log cycle 이상을 나타내어 숙성에 별다른 영향은 없을 것으로 사료되었다. 매실 추출물 1% 첨가구는 김치의 맛, 향, 색상에 영향을 거의 끼치지 않으면서 신맛을 억제시키는 효과가 뚜렷한 것으로 나타났다. 따라서, 매실 추출물을 사용하여 제조한 김치는 본래의 맛에 영향을 끼치지 않으면서 김치의 저장성을 연장시키고 또한 정장작용이 확인되고 있는 김치 유산균과 매실 내에 함유된 여러 가지 유기산을 함께 섭취할 수 있다는 점에서 김치의 품질을 한 단계 더 향상시킬 수 있을 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제26권6호
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• pp.1148-1161
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• 2016

Lactobacilli are universally recognized as probiotics that are widely used in the adjuvant treatment of inflammatory diseases, such as vaginitis and enteritis. With the overuse of antibiotics in recent years, the lactobacilli in the human body are killed, which could disrupt the microecological balance in the human body and affect health adversely. In this work, cephalosporin-resistant Lactobacillus casei RL20 was obtained successfully from the feces of healthy volunteers, which possessed a stable genetic set. However, the shortage of lactic acid (72.0 g/l at 48 h) by fermentation did not meet the requirement for its use in medicine. To increase the production of lactic acid, the functional genes pfk and glk were introduced into the wild strain. A yield of 144.2 g/l lactic acid was obtained in the transgenic L. casei RL20-2 after fermentation for 48 h in 1 L of basic fermentation medium with an initial glucose concentration of 100 g/l and increasing antibacterial activity. These data suggested that L. casei RL20-2 that exhibited a high yield of lactic acid may be a potential probiotic to inhibit the spread of bacterial infectious diseases and may be used for vaginitis therapy.

• 한국식품영양과학회지
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• 제36권11호
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• pp.1425-1430
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• 2007

본 연구에서는 석류식초의 품질관리를 위하여 알코올 및 초산발효 조건을 모니터링하였다. 알코올발효과정에서 석류과즙농도 $16^{\circ}Brix$에서 알코올 함량이 가장 높았으나 석류과즙농도 $12^{\circ}Brix$에서도 과실식초 규격에 적합하였다. 석류 알코올발효과정에서 발효초기 fructose와 glucose가 나타났고 시간이 경과됨에 따라 감소하여 발효 4일째는 glucose만 검출되었다. 유기산은 oxalic, lactic, acetic 및 citric acid가 확인되었으며 알코올발효과정에서는 크게 변화되지 않았다. 초산발효 과정에서 산도는 초기 1.56%에서 5.54%로 증가되었으며, oxalic, lactic 및 citric acid 함량은 크게 변화되지 않고 acetic acid만 증가되었다. 따라서 석류과즙과 석류식초의 citric acid 함량을 비교함으로써 식초의 석류과즙 함량 측정이 가능하였다. 이상의 결과 석류과즙 $12^{\circ}Brix$를 사용하여 알코올 및 초산발효과정으로 석류식초를 제조방법 및 품질관리 기준을 확립하였다.

• 한국식품과학회지
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• 제31권2호
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• pp.488-494
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• 1999

파김치와 배추김치를 제조하여 $10^{\circ}C$와 $20^{\circ}C$로 발효시키면서 시간에 따른 젖산균의 변화와 발효 양상을 비교 조사하였다. pH, 총 산도 및 총 균수의 변화를 조사한 결과 파김치가 배추김치보다 발효가 늦게 진행됨을 알 수 있었다. 또한 총 균수, Leuconostoc 속 젖산균의 최대 균수 및 Lactobacillus 속 젖산균의 최대 균수도 배추김치보다 파김치에서 적었고 이러한 차이는 발효 온도가 낮은 $10^{\circ}C$의 경우 더욱 크게 나타났다. 파김치를 $10^{\circ}C$에서 발효시킨 경우 34일 경과 후에도 발효가 여전히 진행 중이었다. 총 당 함량을 조사한 결과 담금 직후 파김치가 배추김치보다 높았고 발효가 진행됨에 따라 감소하였으나 실험 종료 시점에서도 여전히 파김치가 높은 당 함량을 보여 파김치의 발효가 배추김치보다 느리게 진행되는 것이 총 당 함량이 낮았기 때문이 아님을 알 수 있었다. 적숙기 파김치에서 분리한 주된 젖산균은 Leuconostoc mesenteroides와 Lactobacillus plantarum으로 동정되어 배추김치 발효의 주된 젖산균과 차이가 없는 것으로 나타났다.