• Title/Summary/Keyword: laboratory model

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Comapartive Study on the Growth of Bay Scallop, Argopecten irradians, in Three Rearing Sites (해만가리비, Argopecten irradians의 해역별 성장)

  • Oh, Bong-Sae;Yang, Moon-Ho;Jung, Choon-Goo;Kim, Young-Sook;Kim, Sook-Yang;Kim, Sung-Yeon
    • The Korean Journal of Malacology
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    • v.19 no.2
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    • pp.143-152
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    • 2003
  • Water temperature during the culturing period was 10.4-25.5$^{\circ}C$ and there was a little difference between rearing sites. Salinity (25.00-31.17 psu) and DO (over 6.13 mg/l) showed reasonable condition for the growth of bay scallop. Concentrations of chlorophyll-a were ranged 1.69-7.40 $^{\mu}$g/l, and they fluctuated monthly in the every sampling site. During the growing period from June to October, dominant phytoplankton species were Ceratium sp. in Nammeon and Hoejin, Chaetoceros sp. in Dolsan. Density of phytoplankton was high in July, August and October, but it was low in September and November. When young bay scallops were cultured for 185 days in Nammyeon, Hoejin and Dolsan, shell heights were grown for 0.19 mm/day, 0.18 mm/day and 0.16 mm/day, respectively, and total weights were increased 0.16 g/day, 0.16 g/day and 0.13 g/day, respectively. Daily growth rates of shell height were 0.606%, 0.581% and 0.549%, and daily growth rates of total weight were 1.972%, 1.857% and 1.746%, respectively. Maximum predicted values of shell height calculated by von Bertalanffy growth model were 52.62 mm, 51.74 mm and 48.91 mm, respectively. Survival rate was the highest in Nammyeon (87.0%), but the rates in Hoejin and Dolsan sharply decreased after November.

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Inherent Optical Properties of Red Tide Algal for Ocean Color Remote Sensing Application (해색원격탐사 활용을 위한 적조생물종 고유 광특성 연구)

  • Ahn, Yu-Hwan;Moon, Jeong-Eon;Seo, Won-Chan;Yoon, Hong-Joo
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.12 no.1
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    • pp.47-54
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    • 2009
  • This research is about the inherent optical properties(IOPs) of algae which is collected from Nam-Hae for basic research of red tide remote sensing technique development. 21 kinds of red tide organisms were cultivated to investigate IOPs of them in the level of laboratory, and specific absorption coefficient of phytoplankton($a^*$) and backscattering coefficient of phytoplankton(${b_b}^*$) are estimated by using spectrophotometer. Absorption spectrums according to species appeared to range from 0.005 to 0.06 ($m^2{\cdot}mg^{-1}$), and the shapes of spectrums were also different. The range of ${b_b}^*$ appeared to be $10^{-2}{\sim}10^{-4}\;m^2{\cdot}mg^{-1}$, which had about 100 times differences between species, and the shape of spectrum have significant difference between species. These results will input as a remote sensing reflectance model input parameter from ocean color.

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Effects of Temperature on the Uptake and Retention of Cesium-137 by the Clam Cyclina sinensis (가무락조개에 의한 세슘-137 의 농축(濃縮)과 잔류(殘留)에 미치는 온도(溫度)의 영향)

  • Yoo, Byung-Sun;Lee, Jeong-Ho;Lee, Su-Rae
    • Korean Journal of Environmental Agriculture
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    • v.2 no.1
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    • pp.24-29
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    • 1983
  • The effects of temperature on the uptake of $^{137}Cs$ from seawater and on the retention after its uptake by the clam Cycling sinensis was investigated under laboratory conditions. The clams exhibited a greater bioaccumulation of $^{137}Cs$ in $25^{\circ}C$-acclimated animals than those acclimated at $15^{\circ}C$. The viscera of the clams reached the highest bioconcentration factor after 14 days uptake from seawater, but the tissue distribution pattern of $^{137}Cs$ was little influenced, if any, by the uptake temperature. The uptake rate slightly decreased with an increase of temperature in order of $10^{\circ}C$. The radionuclide accumulated in clams was released again in a radionuclide-free seawater according to a two-exponential compartment model. A temperature increase of $10^{\circ}C$ reduced the biological half-life of the long-lived component with a factor of about two, whereas it caused no change in the short-lived component.

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A study on development of CRM by means of XRF analysis for fine ceramic ($BaTiO_3$) (티탄산바륨 소재의 XRF 분석용 CRM 개발에 관한 연구)

  • Kim, Young Man;Jeong, Chan Yee;Lim, Chang Ho;Song, Taek Yong;Lee, Dong Soo
    • Analytical Science and Technology
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    • v.9 no.4
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    • pp.382-391
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    • 1996
  • In this study, 12 different chemical species of fine ceramic($BaTiO_3$) were synthesized as the standard materials for the fast and accurate measurements of X-ray fluorescence spectrometry. Samples were diluted to sixteen times with the filling compound ($Li_2B_4O_7+LiBO_2$) in order to remove the matrix effect, and to get the convenient storage and homogeneity of ingredients. The matrix effects among the ingredients were corrected by the empirical coefficient method based on the Lucas-Tooth and Price model. The standard curve on 12 standard materials containing 15 elements were obtained by using X-ray fluorescence spectrometry at three different laboratories. The correlation factors of BaO, PbO, SrO, $Fe_2O_3$, $La_2O_3$, $SnO_2$, ZnO, $ZrO_2$, CaO indicated the relati vely good agreement over 0.995 among the three different laboratories. $SiO_2$ and $Al_2O_3$ showed the poor linearity because of their low fluorescence intensities.

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Long-term Trend Analysis of Key Criteria Air Pollutants over Air Quality Control Regions in South Korea using Observation Data and Air Quality Simulation (관측자료와 대기질 모사를 이용한 주요 기준성 대기오염물질의 권역별 장기변화 분석)

  • Ju, Hyeji;Kim, Hyun Cheol;Kim, Byeong-Uk;Ghim, Young Sung;Shin, Hye Jung;Kim, Soontae
    • Journal of Korean Society for Atmospheric Environment
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    • v.34 no.1
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    • pp.101-119
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    • 2018
  • In this study, we analyzed long-term measurements and air quality simulation results of four criteria air pollutants ($PM_{10}$, $O_3$, $NO_2$, and $SO_2$) for 10 years, from 2006 to 2015, with emphasis on trends of annual variabilities. With the observation data, we conducted spatial interpolation using the Kriging method to estimate spatial distribution of pollutant concentrations. We also performed air quality simulations using the CMAQ model to consider the nonlinearity of the secondary air pollutants such as $O_3$ and the influence of long-range transport. In addition, these simulations are used to deduce the effect of long-term meteorological variations on trends of air quality changes because we fixed the emissions inventory while changing meteorological inputs. The nation-wide inter-annual variability of modeled $PM_{10}$ concentrations was $-0.11{\mu}g/m^3/yr$, while that of observed concentrations was $-0.84{\mu}g/m^3/yr$. For the Seoul Metropolitan Area, the inter-annual variability of observed $PM_{10}$ concentrations was $-1.64{\mu}g/m^3/yr$ that is two times rapid improvement compared to other regions. On the other hand, the inter-annual variability of observed $O_3$ concentrations is 0.62 ppb/yr which is larger than the simulated result of 0.13 ppb/yr. Magnitudes of differences between the modeled and observed inter-annual variabilities indicated that decreasing trend of $PM_{10}$ and increasing trend of $O_3$ are more influenced by emissions and oxidation states than meteorological conditions. We also found similar patterns in $NO_2$. However, $NO_2$ trends showed greater regional and seasonal differences than other pollutants. The analytic approach used in this study can be applicable to estimate changes in factors determining air quality such as emissions, weather, and surrounding conditions over a long term. Then analysis results can be used as important data for air quality management planning and evaluation of the chronic impact of air quality.

Real-Time RT-PCR on SAG1 and BAG1 Gene Expression during Stage Conversion in Immunosuppressed Mice Infected with Toxoplasma gondii Tehran Strain

  • Selseleh, Monavar;Modarressi, Mohammad Hossein;Mohebali, Mehdi;Shojaee, Saeedeh;Eshragian, Mohammad Reza;Selseleh, Mina;Azizi, Ebrahim;Keshavarz, Hossein
    • Parasites, Hosts and Diseases
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    • v.50 no.3
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    • pp.199-205
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    • 2012
  • Toxoplasmic encephalitis is caused by reactivation of bradyzoites to rapidly dividing tachyzoites of the apicomplexan parasite Toxoplasma gondii in immunocompromised hosts. Diagnosis of this life-threatening disease is problematic, because it is difficult to discriminate between these 2 stages. Toxoplasma PCR assays using gDNA as a template have been unable to discriminate between an increase or decrease in SAG1 and BAG1 expression between the active tachyzoite stage and the latent bradyzoite stage. In the present study, real-time RT-PCR assay was used to detect the expression of bradyzoite (BAG1)- and tachyzoite-specific genes (SAG1) during bradyzoite/tachyzoite stage conversion in mice infected with T. gondii Tehran strain after dexamethasone sodium phosphate (DXM) administration. The conversion reaction was observed in the lungs and brain tissues of experimental mice, indicated by SAG1 expression at day 6 after DXM administration, and continued until day 14. Bradyzoites were also detected in both organs throughout the study; however, it decreased at day 14 significantly. It is suggested that during the reactivation period, bradyzoites not only escape from the cysts and reinvade neighboring cells as tachyzoites, but also converted to new bradyzoites. In summary, the real-time RT-PCR assay provided a reliable, fast, and quantitative way of detecting T. gondii reactivation in an animal model. Thus, this method may be useful for diagnosing stage conversion in clinical specimens of immunocompromised patients (HIV or transplant patients) for early identification of tachyzoite-bradyzoite stage conversion.

Augmenter of Liver Regeneration Alleviates Renal Hypoxia-Reoxygenation Injury by Regulating Mitochondrial Dynamics in Renal Tubular Epithelial Cells

  • Long, Rui-ting;Peng, Jun-bo;Huang, Li-li;Jiang, Gui-ping;Liao, Yue-juan;Sun, Hang;Hu, Yu-dong;Liao, Xiao-hui
    • Molecules and Cells
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    • v.42 no.12
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    • pp.893-905
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    • 2019
  • Mitochondria are highly dynamic organelles that constantly undergo fission and fusion processes that closely related to their function. Disruption of mitochondrial dynamics has been demonstrated in acute kidney injury (AKI), which could eventually result in cell injury and death. Previously, we reported that augmenter of liver regeneration (ALR) alleviates renal tubular epithelial cell injury. Here, we gained further insights into whether the renoprotective roles of ALR are associated with mitochondrial dynamics. Changes in mitochondrial dynamics were examined in experimental models of renal ischemia-reperfusion (IR). In a model of hypoxia-reoxygenation (HR) injury in vitro, dynamin-related protein 1 (Drp1) and mitochondrial fission process protein 1 (MTFP1), two key proteins of mitochondrial fission, were downregulated in the Lv-ALR + HR group. ALR overexpression additionally had an impact on phosphorylation of Drp1 Ser637 during AKI. The inner membrane fusion protein, Optic Atrophy 1 (OPA1), was significantly increased whereas levels of outer membrane fusion proteins Mitofusin-1 and -2 (Mfn1, Mfn2) were not affected in the Lv-ALR + HR group, compared with the control group. Furthermore, the mTOR/4E-BP1 signaling pathway was highly activated in the Lv-ALR + HR group. ALR overexpression led to suppression of HR-induced apoptosis. Our collective findings indicate that ALR gene transfection alleviates mitochondrial injury, possibly through inhibiting fission and promoting fusion of the mitochondrial inner membrane, both of which contribute to reduction of HK-2 cell apoptosis. Additionally, fission processes are potentially mediated by promoting tubular cell survival through activating the mTOR/4E-BP1 signaling pathway.

An Experimental Study on the Variation of Hydraulic Characteristics due to Vegetation in Open Channel (개수로에서 식생에 의한 수리특성 변화에 관한 실험적 연구)

  • Lee, Joon-Ho;Yoon, Sei-Eui
    • Journal of Korea Water Resources Association
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    • v.40 no.3
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    • pp.265-276
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    • 2007
  • An understanding of the hydraulic characteristics in the compound channel with vegetation is important in designing stream restorations or managing the floodplain. A laboratory flume of 16 m long and 0.8 m wide was used for analysis of the hydraulic characteristics in the single section channel and the compound channel with artificial vegetation. Slope of experimental channel is 0.5 %. Discharges are ranged from $0.2\;m^3/s\;to\;$0.4\;m^3/s$. The experiments were done by changing water depth ratio, vegetation density and vegetation location. When water depth ratio in the single section channel with vegetation increase up to 3.5, the results showed that the increment of water depth due to vegetation may be ignored in practice. The maximum increment of water depth was measured up to 6 % in the compound channel with vegetation and the range of velocities increment in the low flow channel was from 25 % to 85 % compared with section average velocities. As the vegetation densities increase and water depth ratios decrease, the velocity of the low flow channel increased. The range of roughness coefficients in the vegetated reaches were estimated from 0.055 to 0.14 in the single section channel and from 0.063 to 0.085 in the compound channel using HEC-RAS and RMA-2 model.

근적외 분광분석법을 이용한 황색종 잎담배의 화학성분 분석

  • 김용옥;이경구;장기철;김기환
    • Journal of the Korean Society of Tobacco Science
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    • v.20 no.2
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    • pp.183-190
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    • 1998
  • This study was conducted to analyze chemical components in flue-cured tobacco using near infrared spectroscopy(NIRS). Samples were collected in '96 and '97 crop year and were scanned in the wavelengths of 400 ~ 2500 nm by near infrared analyzer(NIRSystem Co., Model 6500). Calibration equations were developed and then analyzed flue-cured samples by NIRS. The standard error of calibration(SEC) and performance (SEP) of '96 crop year samples between NIRS and standard laboratory analysis(SLA) were 0.18% and 0.24% for nicotine, 1.60% and 1.77% for total sugar, 0.13% and 0.15% for total nitrogen, 0.58% and 0.68% for crude ash, 0.23% and 0.28% for ether extracts, and 0.09% and 0.08% for chlorine, respectively. The coefficient of determination($R^2$) of calibration and prediction samples between NIRS and SLA of '96 crop year samples was 0.94~0.99 and 0.83~0.97 depending on chemical components, respectively. The SEC and SEP of '97 crop year samples were similar to those of '96 crop year samples. The SEP of '97 crop year samples which were analyzed using '96 calibration equation was 0.32 % for nicotine, 2.72% for total sugar, 0.14 % for total nitrogen, 1.00 % for crude ash, 0.48 for ether extracts and 0.17% for chlorine, respectively. The prediction result was more accurate when calibration and prediction samples were produced in the same crop year than those of the different crop year. The SEP of '96 and '97 crop year samples using calibration equation which was developed '96 plus '97 crop year samples was similar to that of '96 crop year samples using 96 calibration equation and that of '97 crop year samples using '97 calibration equation, respectively. The SEP of '97 crop year samples using calibration equation which was developed '96 plus '97 crop year samples was lower than that of '97 crop year samples analyzed by '96 calibration equation. To improve the analytical inaccuracy caused by the difference of crop year between calibration and prediction samples, we need to include the prediction sample spectra which are different from calibration sample spectra in recalibration sample spectra, and then develop recalibration equation. Although the analytical result using NIR is not as good as SLA, the chemical component analysis using NIR can apply to tobacco leaves, leaf process or tobacco manufacturing process which demand the rapid analytical result.

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Application of SWAT for the Estimation of Soil Loss in the Daecheong Dam Basin (대청댐 유역 토양 침식량 산정을 위한 SWAT 모델의 적용)

  • Ye, Lyeong;Yoon, Sung-Wan;Chung, Se-Woong
    • Journal of Korea Water Resources Association
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    • v.41 no.2
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    • pp.149-162
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    • 2008
  • The Soil and Water Assessment Tool (SWAT) developed by the USDA-Agricultural Research Service for the prediction of land management impact on water, sediment, and agricultural chemical yields in a large-scale basin was applied to Daecheong Reservoir basin to estimate the amount of soil losses from different land uses. The research outcomes provide important indications for reservoir managers and policy makers to search alternative watershed management practices for the mitigation of reservoir turbidity flow problems. After calibrations of key model parameters, SWAT showed fairly good performance by adequately simulating observed annual runoff components and replicating the monthly flow regimes in the basin. The specific soil losses from agricultural farm field, forest, urban area, and paddy field were 33.1, $2.3{\sim}5.4$ depending on the tree types, 1.0, and 0.1 tons/ha/yr, respectively in 2004. It was noticed that about 55.3% of the total annual soil loss is caused by agricultural activities although agricultural land occupies only 10% in the basin. Although the soil erosion assessment approach adopted in this study has some extent of uncertainties due to the lack of detailed information on crop types and management activities, the results at least imply that soil erosion control practices for the vulnerable agricultural farm lands can be one of the most effective alternatives to reduce the impact of turbidity flow in the river basin system.