• Title/Summary/Keyword: laboratory method

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In-line Automatic defect inspection and repair method for TFT-LCD production

  • Honoki, Hideyuki;Arai, T.;Edamura, T.;Yoshimura, K.;Nakasu, N.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2007.08a
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    • pp.286-289
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    • 2007
  • We have developed an automated circuit defect inspection and repair method that can be used to improve the yield ratio of TFT-LCD. The method focuses on correcting resist patterns after the development process to ensure shape regularity. We built a prototype system and confirmed that the method is valid.

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Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus

  • Cao, Yuying;Yuan, Li;Yang, Shunli;Shang, Youjun;Yang, Bin;Jing, Zhizhong;Guo, Huichen;Yin, Shuanghui
    • Journal of Veterinary Science
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    • v.23 no.5
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    • pp.32.1-32.11
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    • 2022
  • Background: Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry. Objectives: This study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples. Methods: A spbELISA method was developed based on the recombinant E2 expressed in Escherichia coli. The specificity of this established spbELISA method was evaluated using reference serum samples positive for antibodies against other common infectious diseases. The stability and sensitivity were evaluated using an accelerated thermostability test. Results: The spbELISA successfully detected the antibody levels in swine vaccinated with the C-strain of CSFV. In addition, the detection ability of spbELISA for CSFV antibodies was compared with that of other commercial ELISA kits and validated using an indirect immunofluorescence assay. The results suggested that the spbELISA provides an alternative, stable, and rapid serological detection method suitable for the large-scale screening of CSFV serum antibodies. Conclusions: The spbELISA has practical applications in assessing the vaccination status of large pig herds.

In-line (α,n) source sampling methodology for monte carlo radiation transport simulations

  • Griesheimer, David P.;Pavlou, Andrew T.;Thompson, Jason T.;Holmes, Jesse C.;Zerkle, Michael L.;Caro, Edmund;Joo, Hansem
    • Nuclear Engineering and Technology
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    • v.49 no.6
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    • pp.1199-1210
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    • 2017
  • A new in-line method for sampling neutrons emitted in (${\alpha}$,n) reactions based on alpha particle source information has been developed for continuous-energy Monte Carlo simulations. The new method uses a continuous-slowing-down model coupled with (${\alpha}$,n) cross section data to precompute the expected neutron yield over the alpha particle lifetime. This eliminates the complexity and computational cost associated with explicit charged particle transport. When combined with an integrated alpha particle decay source sampling capability, the proposed method provides an efficient and accurate method for sampling (${\alpha}$,n) neutrons based solely on nuclide inventories in the problem, with no additional user input required. Results from several example calculations show that the proposed method reproduces the (${\alpha}$,n) neutron yields and energy spectra from reference experiments and calculations.

Detection of EBV-encoded Small RNA from Diffuse Large B-cell Lymphoma Patients by RT-PCR Method

  • Yoo, Tae Hyun;Lee, Min Ho;Park, Min;Lee, Jaewang;Woo, Hyun Jun;Kim, Hyun Woo;Yang, Ji Yeong;Kwon, Hye Jin;Yeon, Min Ji;Kim, Jong-Bae
    • Biomedical Science Letters
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    • v.21 no.4
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    • pp.227-232
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    • 2015
  • Epstein-Barr virus (EBV) has a pathogenic role in several lymphomas including diffuse large B-cell lymphoma (DLBCL). In this study, we detected EBV from formalin-fixed paraffin embedded (FFPE) tissues of DLBCL patients by RT-PCR and compared the sensitivity of the RT-PCR method to in situ hybridization (ISH) method. The RNA was extracted from 91 FFPE samples with DLBCL and amplified with primers targeting EBV-encoded small RNA (EBER) by RT-PCR. When using the RT-PCR method, 13 of 91 patients (14.3%) were positive and among these 13 cases, 7 cases (7.7%) were from > 50-year-old patients that is classified as EBV positive DLBCL of the elderly. In previous results using ISH method, 3 of 91 patients (3.3%) were positive and 2 case (2.4%) were older than 50-year-old. These results indicate that RT-PCR method used in this study shows a higher sensitivity than ISH method. The ratio of male versus female among the EBV positive samples was 1.2:1 with the ratio of male higher. If RT-PCR method having high sensitivity is used simultaneously as well as the ISH method providing the information of the EBV positive cellular location, it is expected that EBV will be more accurately detected.

A Rigorous 2D Approximation Technique for 3D Waveguide Structures for BPM Calculations

  • Han, Young-Tak;Shin, Jung-Uk;Kim, Duk-Jun;Park, Sang-Ho;Park, Yoon-Jung;Sung, Hee-Kyung
    • ETRI Journal
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    • v.25 no.6
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    • pp.535-537
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    • 2003
  • We propose a rigorous 2D approximation technique for the 3D waveguide structures; it can minimize the well-known approximation errors of the commonly used effective index method. The main concept of the proposed technique is to compensate for the effective cladding index in the equivalent slab model of the original channel waveguide from the modal effective index calculated by the nonuniform 2D finite difference method. With simulations, we used the proposed technique to calculate the coupling characteristics of a directional coupler by the 2D beam propagation method, and the results were almost exactly the same as the results calculated by the 3D beam propagation method.

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A comparative study of three collocation point methods for odd order stochastic response surface method

  • Li, Dian-Qing;Jiang, Shui-Hua;Cheng, Yong-Gang;Zhou, Chuang-Bing
    • Structural Engineering and Mechanics
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    • v.45 no.5
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    • pp.595-611
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    • 2013
  • This paper aims to compare three collocation point methods associated with the odd order stochastic response surface method (SRSM) in a systematical and quantitative way. The SRSM with the Hermite polynomial chaos is briefly introduced first. Then, three collocation point methods, namely the point method, the root method and the without origin method underlying the odd order SRSMs are highlighted. Three examples are presented to demonstrate the accuracy and efficiency of the three methods. The results indicate that the condition that the Hermite polynomial information matrix evaluated at the collocation points has a full rank should be satisfied to yield reliability results with a sufficient accuracy. The point method and the without origin method are much more efficient than the root method, especially for the reliability problems involving a large number of random variables or requiring complex finite element analysis. The without origin method can also produce sufficiently accurate reliability results in comparison with the point and root methods. Therefore, the origin often used as a collocation point is not absolutely necessary. The odd order SRSMs with the point method and the without origin method are recommended for the reliability analysis due to their computational accuracy and efficiency. The order of SRSM has a significant influence on the results associated with the three collocation point methods. For normal random variables, the SRSM with an order equaling or exceeding the order of a performance function can produce reliability results with a sufficient accuracy. The order of SRSM should significantly exceed the order of the performance function involving strongly non-normal random variables.

An SAD-Based Selective Bi-prediction Method for Fast Motion Estimation in High Efficiency Video Coding

  • Kim, Jongho;Jun, DongSan;Jeong, Seyoon;Cho, Sukhee;Choi, Jin Soo;Kim, Jinwoong;Ahn, Chieteuk
    • ETRI Journal
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    • v.34 no.5
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    • pp.753-758
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    • 2012
  • As the next-generation video coding standard, High Efficiency Video Coding (HEVC) has adopted advanced coding tools despite the increase in computational complexity. In this paper, we propose a selective bi-prediction method to reduce the encoding complexity of HEVC. The proposed method evaluates the statistical property of the sum of absolute differences in the motion estimation process and determines whether bi-prediction is performed. A performance comparison of the complexity reduction is provided to show the effectiveness of the proposed method compared to the HEVC test model version 4.0. On average, 50% of the bi-prediction time can be reduced by the proposed method, while maintaining a negligible bit increment and a minimal loss of image quality.

Analysis and comparison of the 2D/1D and quasi-3D methods with the direct transport code SHARK

  • Zhao, Chen;Peng, Xingjie;Zhang, Hongbo;Zhao, Wenbo;Li, Qing;Chen, Zhang
    • Nuclear Engineering and Technology
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    • v.54 no.1
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    • pp.19-29
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    • 2022
  • The 2D/1D method has become the mainstream of the direct transport calculation considering the balance of accuracy and efficiency. However, the 2D/1D method still suffers from stability issues. Recently, a quasi-3D method has been proposed with axial Legendre expansion. Analysis and comparison of the 2D/1D and quasi-3D method is conducted in theory from the equation derivation. Besides, the C5G7 benchmark, the KUCA benchmark and the macro BEAVRS benchmark are calculated to verify the theory comparisons of these two methods with the direct transport code SHARK. All results show that the quasi-3D method has better stability and accuracy than the 2D/1D method with worse efficiency and memory cost. It provides a new option for direct transport calculation with the quasi-3D method.

Rubbing Process Evaluation Method for The LCD Panel

  • Honoki, Hideyuki;Sakai, Kaoru;Kawabe, Shun'Ichi;Nakasu, Nobuaki
    • 한국정보디스플레이학회:학술대회논문집
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    • 2002.08a
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    • pp.115-117
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    • 2002
  • In order to realize a stable rubbing process for the liquid crystal panel, the authors investigated the quantitative evaluation method of rubbing process uniformity. The proposed method focuses on the relationship between the image quality of the LCD panel for gray scale images and the rubbing uniformity. The proposed method indicates rubbing uniformity using quantitative parameters of spots and hairlines on the LCD panel.

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Follicular Unit Classification Method Using Angle Variation of Boundary Vector for Automatic Hair Implant System

  • Kim, Hwi Gang;Bae, Tae Wuk;Kim, Kyu Hyung;Lee, Hyung Soo;Lee, Soo In
    • ETRI Journal
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    • v.38 no.1
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    • pp.195-205
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    • 2016
  • This paper presents a novel follicular unit (FU) classification method based on an angle variation of a boundary vector according to the number of hairs in several FU images. The recently developed robotic FU harvest system, ARTAS, classifies through digital imaging the FU type based on the number of hairs with defects in the contour and outline profile of the FU of interest. However, this method has a drawback in that the FU classification is inaccurate because it causes unintended defects in the outline profile of the FU. To overcome this drawback, the proposed method classifies the FU's type by the number of variation points that are calculated using an angle variation a boundary vector. The experimental results show that the proposed method is robust and accurate for various FU shapes, compared to the contour-outline profile FU classification method of the ARTAS system.