• Title/Summary/Keyword: laboratory experiments

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Elementary Students' Awareness about Self-directed Learning Experiments at Science Club (과학 동아리에서 경험한 자기 주도적 실험 학습에 대한 초등학생들의 인식)

  • Ju, Eun Jeong;Kim, Heung-Tae
    • Journal of Korean Elementary Science Education
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    • v.35 no.2
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    • pp.253-264
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    • 2016
  • The purpose of this study was to investigate implications of self-directed learning experiments in elementary science education through understanding elementary school students' awareness of their experiences in self-directed learning experiments. Twenty students joined the school science club voluntarily and conducted self-directed learning experiments. We collected data through observation of the experiments, interviews, and questionnaires. The students who participated in the club showed high satisfaction with self-directed learning experiments. The participants were aware that their scientific interest and knowledge, and the confidence in conducting experiments were increased. The students felt positive about the inquiry process of conducting self-directed learning experiments with their own subjects. They also felt a sense of achievement in attempting their experiments in defiance of several failures. The participants realized that the self-directed inquires led to increased declarative and procedural knowledge of science. The students stated that they had some difficulties in coping with the different results contrary to expectations and preparing laboratory materials and instruments. Nonetheless, they showed the promotion of their scientific literacy during overcoming those difficulties. We suggest that self-directed learning experiments can be a more effective way in science learning to make students experience the nature of science than existing school experiments. This can be implemented through a creative experience activities such as science clubs.

Lens Assembling Methods for Near Field Optical Storage Devices (근접장 광 저장 장치를 위한 렌즈 조립)

  • Shin Yun Sup;Park Jin Moo;Lee Jeong Uk;Jeong Mi Hyun;Seo Jeong Kyo;Choi In Ho
    • 정보저장시스템학회:학술대회논문집
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    • 2005.10a
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    • pp.2-3
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    • 2005
  • Air gap control method using a conventional actuator for near field recording technology has been reported. To achieve good performance in the air gap servo and further experiments, the assembling between OL and SIL is the critical procedure. A lens set with NA 1.45 at 405 m has been assembled and tested by using a Twyman-Green interferometer. The tolerance of assembling is very tight and the designing and adjusting procedure must be carefully controlled.

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NEUTRONICS MODELING AND SIMULATION OF SHARP FOR FAST REACTOR ANALYSIS

  • Yang, W.S.;Smith, M.A.;Lee, C.H.;Wollaber, A.;Kaushik, D.;Mohamed, A.S.
    • Nuclear Engineering and Technology
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    • v.42 no.5
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    • pp.520-545
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    • 2010
  • This paper presents the neutronics modeling capabilities of the fast reactor simulation system SHARP, which ANL is developing as part of the U.S. DOE's NEAMS program. We discuss the three transport solvers (PN2ND, SN2ND, and MOCFE) implemented in the UNIC code along with the multigroup cross section generation code $MC^2$-3. We describe the solution methods and modeling capabilities, and discuss the improvement needs for each solver, focusing on massively parallel computation. We present the performance test results against various benchmark problems and ZPR-6 and ZPPR critical experiments. We also discuss weak and strong scalability results for the SN2ND solver on the ZPR-6 critical assembly benchmarks.

Experimental Observations for Anode Optimization of Oxide Reduction Equipment

  • David Horvath;James King;Robert Hoover;Steve Warmann;Ken Marsden;Dalsung Yoon;Steven Herrmann
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.20 no.4
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    • pp.383-398
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    • 2022
  • The electrochemical behavior was investigated during the electrolysis of nickel oxide in LiCl-Li2O salt mixture at 650℃ by changing several components. The focus of this work is to improve anode design and shroud design to increase current densities. The tested components were ceramic anode shroud porosity, porosity size, anode geometry, anode material, and metallic porous anode shroud. The goal of these experiments was to optimize and improve the reduction process. The highest contributors to higher current densities were anode shroud porosity and anode geometry.

ShcD interacts with TrkB via its PTB and SH2 domains and regulates BDNF-induced MAPK activation

  • You, Yuangang;Li, Weiqi;Gong, Yanhua;Yin, Bin;Qiang, Boqin;Yuan, Jiangang;Peng, Xiaozhong
    • BMB Reports
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    • v.43 no.7
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    • pp.485-490
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    • 2010
  • Neurotrophins regulate many aspects of neuronal function through activation of the high affinity Trk receptors. Shc family proteins are implicated in the coupling of RTK to the Ras/mitogen-activated protein kinase signaling cascade. Here we report that the fourth Shc family member, ShcD, associates with TrkB receptor and regulates BDNF-induced MAPK activation. Yeast two-hybrid assay and Co-IP experiments demonstrate ShcD interacts with TrkB in a kinase-activity-dependent manner. Confocal analysis shows ShcD cololizes well with TrkB in transfected 293T cells. Subsequent mapping experiments and mutational analysis indicate that both PTB and SH2 domains are capable of binding to TrkB and PTB domain binds to TrkB NPQY motif. Furthermore, ShcD is involved in BDNF-induced MAPK activation. In summary, we demonstrate that ShcD is a substrate of TrkB and mediates TrkB downstream signaling pathway.

Enhanced Production of Soluble Pyrococcus furiosus α-Amylase in Bacillus subtilis through Chaperone Co-Expression, Heat Treatment and Fermentation Optimization

  • Zhang, Kang;Tan, Ruiting;Yao, Dongbang;Su, Lingqia;Xia, Yongmei;Wu, Jing
    • Journal of Microbiology and Biotechnology
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    • v.31 no.4
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    • pp.570-583
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    • 2021
  • Pyrococcus furiosus α-amylase can hydrolyze α-1,4 linkages in starch and related carbohydrates under hyperthermophilic condition (~ 100℃), showing great potential in a wide range of industrial applications, while its relatively low productivity from heterologous hosts has limited the industrial applications. Bacillus subtilis, a gram-positive bacterium, has been widely used in industrial production for its non-pathogenic and powerful secretory characteristics. This study was conducted to increase production of P. furiosus α-amylase in B. subtilis through three strategies. Initial experiments showed that co-expression of P. furiosus molecular chaperone peptidyl-prolyl cis-trans isomerase through genomic integration mode, using a CRISPR/Cas9 system, increased soluble amylase production. Therefore, considering that native P. furiosus α-amylase is produced within a hyperthermophilic environment and is highly thermostable, heat treatment of intact culture at 90℃ for 15 min was performed, thereby greatly increasing soluble amylase production. After optimization of the culture conditions (nitrogen source, carbon source, metal ion, temperature and pH), experiments in a 3-L fermenter yielded a soluble activity of 3,806.7 U/ml, which was 3.3- and 28.2-fold those of a control without heat treatment (1,155.1 U/ml) and an empty expression vector control (135.1 U/ml), respectively. This represents the highest P. furiosus α-amylase production reported to date and should promote innovation in the starch liquefaction process and related industrial productions. Meanwhile, heat treatment, which may promote folding of aggregated P. furiosus α-amylase into a soluble, active form through the transfer of kinetic energy, may be of general benefit when producing proteins from thermophilic archaea.

Assessment of the severe accident code MIDAC based on FROMA, QUENCH-06&16 experiments

  • Wu, Shihao;Zhang, Yapei;Wang, Dong;Tian, Wenxi;Qiu, Suizheng;Su, G.H.
    • Nuclear Engineering and Technology
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    • v.54 no.2
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    • pp.579-588
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    • 2022
  • In order to meet the needs of domestic reactor severe accident analysis program, a MIDAC (Module Invessel Degraded severe accident Analysis Code) is developed and maintained by Xi'an Jiaotong University. As the accuracy of the calculation results of the analysis program is of great significance for the formulation of severe accident mitigation measures, the article select three experiments to evaluate the updated severe accident models of MIDAC. Among them, QUENCH-06 is the international standard No.45, QUENCH-16 is a test for the analysis of air oxidation, and FROMA is an out-of-pile fuel rod melting experiment recently carried out by Xi'an Jiaotong University. The heating and melting model with lumped parameter method and the steam oxidation model with Cathcart-Pawel and Volchek-Zvonarev correlations combination in MIDAC could better meet the needs of severe accident analysis. Although the influence of nitrogen still need to be further improved, the air oxidation model with NUREG still has the ability to provide guiding significance for engineering practice.

Elementary School Students' Perception about the Aims of School Science Experiments by Gender and Academic Achievement (성별과 학업 성취도에 따른 학교 과학 실험의 목적에 대한 초등학생의 인식)

  • Kim, Ji-Hye;Shin, Young-Joon
    • Journal of Korean Elementary Science Education
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    • v.32 no.2
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    • pp.159-168
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    • 2013
  • The purpose of this study was to investigate the elementary school students' perception about "the aims of school science experiments". For this study, the perceptions were surveyed from 193 elementary school students (96 boys and 97 girls) in one school located in Gyeonggi-Do. The responses were analyzed with categorization based on their gender, school science test scores. The results from this study were as follows; in relation to the aims of school science experiments, the elementary school students considered intrinsic values of doing experiments were more worthy than the extrinsic values of it. It was found that 25.4% of the students had ideas that students do science experiments for understanding science theory. Up to 70% of the students responded that they do science experiments for understanding and memorizing the contents of science textbooks. School science experiments were regarded as an educational tool for the science study. It is recommended that teacher need to make clear the aims of laboratory work and help students to understand of it.

Reformation and Application of an Introductory Chemistry Laboratory at the University for Teacher Education (교사양성 대학에서의 일반화학실험 개선과 적용)

  • Yi, Hwa-Jeong;Kang, Seong-Joo
    • Journal of The Korean Association For Science Education
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    • v.25 no.3
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    • pp.346-352
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    • 2005
  • The purpose of this study was to develop problem-solving experiments and investigate the effects of these experiments on student attitudes. According to results the experiments were effective in changing student attitude from 'dead reckoners' to 'empiricists'. In addition, these experiments effectively changed perceptions about experiments, especially in the sub-categories of difference recognition, degree of inquiry, and use of debate.