• Title/Summary/Keyword: laboratory experiments

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Susceptibility of sweetpotato whitefly, Bemisia tabaci (Homoptera : Aleyrodidae) to commercially registered insecticides in Korea (외래해충인 담배가루이의 약제감수성)

  • Kim, Gil-Hah;Lee, Young-Su;Lee, In-Hwan;Ahn, Ki-Su
    • The Korean Journal of Pesticide Science
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    • v.4 no.1
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    • pp.51-58
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    • 2000
  • These studies were carried out to investigate the toxicities of 43 registered insecticides to the sweetpotato whitefly(Bemisia tabaci, B. biotype). Insecticide activities were evaluated by testing systemic action and residual effect in the laboratory, and control efficacy in the greenhouse. All experiments were tested at the recommended concentration(ppm) of each insecticides. Insect growth regulators (IGRs), pyriproxyfen and teflubenzuron showed >95% ovicidal effect. The insecticides that showed >95% larvicidal activity on 3rd nymphal instars were abamectin, acetamiprid, imidacloprid, pyriproxyfen, and acetamiprid+ ethofenprox. Insecticides with >95% adulticidal activity were abamectin, acetamiprid, diazinon, endosulfan, fenitrothion, imidacloprid, methidathion, pirimiphos-methyl, pymetrozine, spinosad, acetamiprid+ ethofenprox, cartap kydrochloride+buprofezin, and fenpropathrin+fenitrothion. Abamectin, acetamiprid, imidacloprid, pyriproxyfen, and acetamiprid+ethofenprox showed both residual effect and systemic activity. In the control efficacy test on B. tabaci, 90% control values were obtained at 1st day after treatment of the insecticides including abamectin, acetamiprid, imidacloprid, pyriproxyfen and acetamiprid+ethofenprox but in pyriproxyfen, 90% control value was reached at 7th day after treatment. These results indicate that abamectin, acetamiprid, imidacloprid, pyriproxyfen and acetamiprid+ethofenprox can be used in control for B. tabaci in field.

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Filtration Characteristics of Paticulate Matter at Bag Filters Coated with PTFE Membrane During Off-Line Pulsing (PTFE membrane이 코팅된 여과백의 off-line 탈진시 미세먼지 집진 특성)

  • Kim, Joung-Hun;Moon, Il-Shik;Hwang, Min-Young;Kim, Ryang-Gyoon;Ko, Daekwun
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.7
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    • pp.391-402
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    • 2017
  • Particulate matter becomes an important issue in the environmental society recently so that it is necessary to evaluate that the commercial application of baghouse systems for effective control of fine particulates is viable. A laboratory-scale baghouse experimental apparatus with filter bags made of PTFE felt or PTFE felt coated with PTFE membrane is used to investigate the filtration performances of fine particulates. Experiments by changing filtration velocity, inlet dust concentration, and average dust particle size show that the dust collection efficiency becomes higher at lower filtration velocity, higher inlet dust concentration and larger average dust particle size. The total pressure drop through the filter media and dust layer becomes higher at higher filtration velocity and higher inlet dust concentration. The dust collection efficiency is higher and the pressure drop is lower at a baghouse with filter bags coated with PTFE membrane than that without membrane coating. From the result that the dust collection efficiency of $PM_{2.5}$ in a reasonable filtration velocity range during off-line pulsing at a baghouse with PTFE felt bag filters coated with PTFE membrane is as high as 99.99%, it is confirmed that the use of baghouse is an effective measure to control the fine particulates.

Gas Migration in Low- and Intermediate-Level Waste (LILW) Disposal Facility in Korea (중·저준위 방사성폐기물 처분시설 폐쇄후 기체이동)

  • Ha, Jaechul;Lee, Jeong-Hwan;Jung, Haeryong;Kim, Juyub;Kim, Juyoul
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.12 no.4
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    • pp.267-274
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    • 2014
  • The first Low- and Intermediate-Level Waste (LILW) disposal facility with 6 silos has been constructed in granite host rock saturated with groundwater in Korea. A two-dimensional numerical modeling on gas migration was carried out using TOUGH2 with EOS5 module in the disposal facility. Laboratory-scale experiments were also performed to measure the important properties of silo concrete related with gas migration. The gas entry pressure and relative gas permeability of the concrete was determined to be $0.97{\pm}0.15bar$ and $2.44{\times}10^{-17}m^2$, respectively. The results of the numerical modeling showed that hydrogen gas generated from radioactive wastes was dissolved in groundwater and migrated to biosphere as an aqueous phase. Only a small portion of hydrogen appeared as a gas phase after 1,000 years of gas generation. The results strongly suggested that hydrogen gas does not accumulate inside the disposal facility as a gas phase. Therefore, it is expected that there would be no harmful effects on the integrity of the silo concrete due to gas generation.

Studies on In Vitro Fertilization and Development of In Vitro Matured Porcine Follicular Oocytes I. Effect of Media and Capacitation Procedure on In Vitro Fertilization (체외성숙 돼지난포란의 체외수정과 배발달에 관한 연구 I. 배양액, 수정능획득 방법이 체외성숙 난포란의 체외성숙에 미치는 영향)

  • 정형민;엄상준;승경록;이훈택;정길생
    • Korean Journal of Animal Reproduction
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    • v.17 no.2
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    • pp.103-111
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    • 1993
  • These experiments were undertaken to establish the optimal culture systems for in vitro maturation, fertilization and subsequently embryonic development of porcine immature follicular oocytes isolated from the ovary of slaughtered pigs. Porcine ovaries were brought to the laboratory from local slaughter house within 1 hour after slaughtering and cumulus oocytes complexes were recovered from antral follicles (3~5mm) with 23 gauge needle. To maturate follicular oocytes, cumulus oocytes complexes were washed three times with TCM-199 containing 25mM HEPES and incubated (39$^{\circ}C$, 5% CO2 in air) for 42hrs. Ejaculated and liquid storaged boar spermatozoa capacitated with different sperm capacitation methods and media were prepared forfertilizaing of matured follicular oocytes in vitro. Fertilization was performed by adding 5~10${mu}ell$ of capacitated spermatozoa containing 1~5$\times$105 sperm/ml to droplets. Eighteen to twenty-eight hours after sperm insemination, fertilized eggs were washed three times with culture media and transferred to the culture media. The fertilization rates of in vitro matured follicular oocytes cultured in B. O., TCM-HEPES, m-KRB, and TALP-II media were 61.3%, 83.0%, 88.9% and 89.2%, respectively. In addition, the polyspermy rates were 60.7%, 66.5%, 53.8%, and 43.9%, respectively. These data indicated that the highest of fertilization and the lowest of polyspermy rate was shown in TALP-II medium. Spermatozoa capacitated by caffeine, heparin, and percoll density gradient treatment in the 4 different media, the fertilization rates were 33.0~57.2%, 39.9~90.2%, and 52.6~92.8%, respectively, showing the lowest rate in caffeine treatment. The development rate of follicular oocytes, fertilized with the spermatozoa capacitated by caffeine, heparin, and percoll gradient in the TALP-II medium, upto 2 to 4-cell stages were 32.6%, 74.5% and 70.9%, respectively. Finally, fertilization rates of follicular oocytes cultured with follicular fluid containing medium from 10 to 100% were 61.2~94.1% and the rates (90~94%) with 10~20% follicular fluids were significantly higher than those (85.3%) of cultured in the media without follicular fluid. In addition, the rates of pronucleus formation were also higher in follicular fluid treated group (73.1~83.0%) than those (64.7%) of oocytes cultured without follicular fluid. The highest fertilization and pronucleus formation rates was found in oocytes cultured with 10% follicular fluid. These results suggest that the addition of heparin or percoll density gradient method is better capacitation method. Furthermore, the addition of porcine follicular fluid to the fertilization medium may improve the fertilization rates and formation of pronucleus.

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Effects of Root Zone Applications of Some Systemic Insecticides for Control of the Brown Planthopper, Nilaparvata lugens ($St{\aa}l$) (Homoptera: Delphacidae) (벼멸구에 대한 여러 가지 침투성 살충제의 근부처리 효과)

  • Pham, Hong-Hien;Kim, Jong-Kyu;Choi, Byeong-Ryeol;Song, Yoo-Han
    • The Korean Journal of Pesticide Science
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    • v.12 no.3
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    • pp.236-242
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    • 2008
  • Laboratory and greenhouse experiments were conducted to evaluate the effect of root zone application of some systemic insecticides for control of the brown planthopper (BPH), Nilaparvata lugens, as one of the management option to minimize the adverse effects and maximize the efficacy of insecticide application. Five systemic insecticides, namely, carbofuran, carbosulfan, diazinon, ethoprophos and imidacloprid, as granular formulation were placed in the root zone and measured the mortality, fecundity, and nymphal survivorship of the planthopper. Diazinon and ethoprophos did not show the significant mortality of the BPH. When the BPH were inoculated at the day of carbofuran treatment, adult mortality was shown almost 100% at seven days after treatment and BPH nymphs were remained very few on rice at 25 days after treatment. When carbofuran were placed 10 days before the BPH inoculation, it showed almost 100% adult mortality after one day, and no nymphs were found until 25 day after inoculation. Efficacy of carbofuran on BPH when applied in 10 day-old rice was higher than in 30 day-old rice. These results indicated that the root zone application of carbofuran can control N. lugens effectively with less adverse effect to the natural enemies inhabited on rice plants.

INTEGRATED RAY TRACING MODEL FOR END-TO-END PERFORMANCE VERIFICATION OF AMON-RA INSTRUMENT (AMON-RA 광학계를 활용한 통합적 광선 추적 기법의 지구 반사율 측정 성능 검증)

  • Lee, Jae-Min;Park, Won-Hyun;Ham, Sun-Jeong;Yi, Hyun-Su;Yoon, Jee-Yeon;Kim, Sug-Whan;Choi, Ki-Hyuk;Kim, Zeen-Chul;Lockwood, Mike
    • Journal of Astronomy and Space Sciences
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    • v.24 no.1
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    • pp.69-78
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    • 2007
  • The international EARTHSHINE mission is to measure 1% anomaly of the Earth global albedo and total solar irradiance using Amon-Ra instrument around Lagrange point 1. We developed a new ray truing based integrated end-to-end simulation tool that overcomes the shortcomings of the existing end-to-end performance simulation techniques. We then studied the in-orbit radiometric performance of the breadboard Anon-Ra visible channel optical system. The TSI variation and the Earth albedo anomaly, reported elsewhere, were used as the key input variables in the simulation. The output flux at the instrument focal plane confirms that the integrated ray tracing based end-to-end science simulation delivers the correct level of incident power to the Amon-Ra instrument well within the required measurement error budget of better than ${\pm}0.28%$. Using the global angular distribution model (ADM), the incident flux is then used to estimate the Earth global albedo and the TSI variation, confirming the validity of the primary science cases at the L1 halo orbit. These results imply that the integrated end-to-end ray tracing technique, reported here, can serve as an effective and powerful building block of the on-line science analysis tool in support of the international EARTHSHINE mission currently being developed.

Hydraulic Characteristics of Anaerobic Fluidized Bed Bioreactor (혐기성 유동상 반응기의 수리학적 특성)

  • Seok, Jong-Hyuk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.2
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    • pp.90-96
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    • 2018
  • Tracer experiments were carried out on two laboratory modes, "without media mode" and "with media mode", to examine the hydraulic characteristics of the anaerobic fluidized bed bioreactor (AFBR). For both configurations, a formula was derived for the hydraulics and data interpretation to obtain the actual characteristics of the reactor. The dispersion model is based on the assumption that carriers are non-reacting and the dispersion coefficient is constant. The model represents the one-dimensional unsteady-state concentration distribution of the non-reacting tracer in the reactors. The experimental results showed that the media increased the mixing conditions in the reactor considerably. For the reactor without media, in the range tested, the dispersion coefficient was at least an order of magnitude smaller than that of the reactor with media. Advective transport dominates and the flow pattern approaches the plug flow reactor (PFR) regime. The dispersion coefficient increased significantly as us, the superficial liquid velocity, was increased proportionally to 0.82cm/s. On the other hand, for the reactor with media, the flow pattern was in between a PFR and a completely mixed flow reactor (CMFR) regime, and the dispersion coefficient was saturated at us=0.41cm/s, remaining relatively constant, even at us=0.82cm/s. The dispersion coefficient depends strongly on the liquid Reynolds number (Re) or the particle Reynolds number (Rep) over the range tested.

Forward probing utilizing electrical resistivity and induced polarization for predicting mixed-ground ahead of TBM tunnel face (전기비저항과 유도분극을 활용한 TBM 터널 굴착면 전방 복합지반 예측 기법)

  • Ryu, Jinwoo;Park, Jinho;Lee, Seong-Won;Lee, In-Mo;Kim, Byung-Kyu
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.20 no.1
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    • pp.55-72
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    • 2018
  • A method that can predict the mixed-ground condition ahead of a TBM tunnel face during tunnel construction utilizing electrical resistivity and induced polarization (IP) was proposed in this study. Effect of TBM advancement approaching the mixed-ground condition (composed of soil layer overlying rock layer) when currently running through soil zone on the electrical resistivity and IP measuring was assessed with laboratory-scale experiments. The resistivity and IP values were measured using four electrodes, by installing two electrodes on the tunnel face (at the cutterhead), and the other two electrodes on the segment lining. The test results showed that both of the measured resistivity and IP values were kept increasing as the TBM is approaching the soil-rock mixed-ground. Also, to get the more reliable results for predicting the mixed-ground condition, it was recommended that the measurement is made at the tunnel face utilizing 4-electrodes installed at the cutterhead as well as it is made utilizing the 2-electrodes installed at the segment lining along with the 2-electrodes installed on the tunnel face (at the cutterhead) so that two measured results are compared each other.

Dewaterability Improvement and Volume Reduction of Bio-Solid using Ultrasonic Treatment (Bio-Solid의 탈수성 개선 및 감량화를 위한 초음파 적용)

  • Park, Cheol;Ha, Jun Soo;Kim, Young Uk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.6
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    • pp.4019-4023
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    • 2014
  • This study examined the effectiveness of ultrasound on enhancing the dewaterability and volume reduction of bio solids from a waste treatment plant. The test specimen was obtained from a storage tank immediately before the dewatering process at a local treatment plant. The test conditions included the energy levels of ultrasonic waves and treatment time. The tests were undertaken using three types of different treatment processors (7 liter, 1 ton, 7 ton container). The capillary suction time (CST) and the viscosity of sludge, which is one of the influencing factors for dewaterbility, were obtained under various test conditions. The results showed that ultrasound increases the CST of the raw specimens, whereas a significant reduction (20 % of the maximum value) of CST occurred in the sample with ultrasound and flocculent. The decrease in viscosity reached 40 % of the maximum value. A centrifugal test was performed to examine the characteristics of the sludge settlement. The settling rate and time required to reach the final values were both enhanced by the ultrasonic energy. An ultrasonic treatment is potentially useful tool for reducing the amount of released sludge. To examine the possible use of field application, the real scale sonic processor was designed and operated. The results were similar (50 % of the maximum value) to those of laboratory experiments.

Egg Production of the Copepod Acartia steueri in Ilkwang Bay, Southeastern Coast of Korea (한국 동해 남부 연안 일광만의 요각류 Acartia steueri의 알 생산력)

  • KANG Hyung-Ku;KANG Yong Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.2
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    • pp.288-295
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    • 1998
  • Egg production of Acartia steueri was estimated in llkwang Bay, located in the southeastern coast of Korea. The equation, combining fecundity, temperature and chlorophyll a, obtained under the laboratory experiments, was applied to the in situ temperature and chlorophyll a for the estimation of field egg production. Mean egg size was $80.52{\mu}m$. Prosome length of adult females was not correlated with egg size. Egg production of field population ranged from 0.32 to $63.32{\mu}gC\;m^{-3}d^{-1}$ with a mean of $13.33{\mu}gC\;m^{-3}d^{-1}$, which were equivalent to $7.1\~1407.1\;eggs\;m^{-3}d^{-1}$ and $296.1\;eggs\;m^{-3}d^{-1}$, respectively. Fecundity of adult females ranged from 5.4 to $12.5\;eggs\;female^{-1}d{-1}$ with a mean of $8\;eggs\;female^{-1}d^{-1}$. Specific egg production rates ranged from 0.028 to 0.117 $d^{-1}$ with a mean of 0.064 $d^{-1}$. Considering the egg Production ($\%$ female body carbon) as a function of temperature and chlorophlrll a concentration, our results showed rather low fecundity, which might be underestimated. Probable cannibalism of egg by the adults were thought to be pan of the reason for this low estimated fecundity.

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