• Title/Summary/Keyword: lab coat

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Detection of 881A→881G Mutation in Tyrosinase Gene and Associations with the Black Ear Coat Color in Rabbits

  • Jiang, Y.L.;Fan, X.Z.;Lu, Z.X.;Tang, H.;Xu, J.-Q.;Du, L.-X.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.10
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    • pp.1395-1397
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    • 2002
  • The tyrosinase gene was selected as a candidate for uncovering genetic mechanism causing 'black ear' coat color in rabbits. A PCR-SSCP detection method was established for the $881^A{\rightarrow}881^G$ mutation located in the central region of the tyrosinase gene between the CuA and CuB binding region signatures, and this was confirmed by sequencing and alignment. Fully consistent associations between the SNP and 'black ear' coat color were observed by analysis in a "black ear" pedigree and on 61 unrelated individuals. This SNP can serve as a molecular marker for use in "back ear" wool rabbit breeding.

Evaluation of Degradation Characteristics of Thermal Barrier Coating on Gas Turbine Blades

  • Jung, Yongchan;Kim, Mintae;Lee, Juhyeung;Ahn, Jamin;Kim, Kihong
    • KEPCO Journal on Electric Power and Energy
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    • v.2 no.2
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    • pp.273-278
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    • 2016
  • In order to evaluate the lifespan of high-temperature parts with thermal barrier coating in gas turbines used for power generation, this study was performed on an 80 MW-class gas turbine exceeding 24 k equivalent operating hours. Degradation characteristics were evaluated by analyzing the YSZ (Yttria Stabilized Zirconia) top coat, which serves as the thermal barrier coating layer, the NiCrAlY bond coat, and interface layers. Microstructural analysis of the top, middle, and bottom sections showed that Thermal Growth Oxide (TGO) growth, Cr precipitate growth within the bond coat layer, and formation of diffusion layer occur actively in high-temperature sections. These microstructural changes were consistent with damaged areas of the thermal barrier coating layer observed at the surface of the used blade. The distribution of Cr precipitates within the bond coat layer, in addition to the thickness of TGO, is regarded as a key indicator in the evaluation of degradation characteristics.

The Effects of Elementary School Students' Experiences in Wearing a Lap Coat on Science-related Attitudes and Perception of Wearing a Lap Coat in Science Learning (과학수업에서 초등학생들의 실험복 착용 경험이 과학 관련 태도와 실험복에 대한 인식에 미치는 영향)

  • Park, Hyoung-Min;Lim, Chae-Seong
    • Journal of Korean Elementary Science Education
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    • v.37 no.4
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    • pp.363-371
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    • 2018
  • The purpose of this study was to analyze the effects of wearing lab coats on science-related attitudes of elementary school students in science learning. In addition, we analyzed the effects of wearing lab coats on perceptions of the authenticity of school science activities and the necessity of wearing lab coats. Samples of the study were composed of 4 classes of 92 fifth graders in Seoul, Korea. Unit of 'Temperature and Heat' was selected as a object of the research. The major results of the study are as follows. First, for the independent t-test of science-related attitudes, a statistically significant difference between the experimental group and the comparative group was found. Second, 9 students (9.78%) were more than 1SD above the average of the difference between the results of pre and post. Seven of them were experimental group students and two were comparative group students. Third, there was a statistically significant difference between the comparison group and the experimental group as a result of the perception test of the similarity of the scientific activity of the elementary school students and the scientific activity of the scientists. Fourth, there was no statistically significant difference between the comparison group and the experimental group as a result of the perception test of the necessity of wearing a lab coat. Based on these findings of this study, implications for science education research and teaching and learning at school are discussed.

Potential Exposure of Nanoparticles from Laboratory to Office (실험실에서 사무실로의 나노입자의 잠재적 노출)

  • Shin, Hyeokjin;Kim, Younghun
    • Clean Technology
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    • v.28 no.2
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    • pp.123-130
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    • 2022
  • Nanoparticles are used in various fields such as chemistry, medicine, the environment, and information and communication. With the increasing use of engineered nanomaterials, exposure to nanoparticles is expected to increase in the workplace and the environmental media. However, while nanotechnology industries are expanding, research on the exposure assessment of nanomaterials to humans and the environment is only at a beginning stage. Especially, if nanoparticles with a size of 100 nm or less that are contained in nano-products are released unintentionally, they may pose potential risks to the human body through breathing or skin exposure. Therefore, in this work, the possibility of potential exposure of nanoparticles moving from the laboratory to the office was confirmed, and nanoparticle safety guidelines are proposed. A nano-collector was used to detect nanoparticles in the atmosphere, and through use of a scanning mobility particle sizer it was found that nanoparticle concentrations in the laboratory and the office tended to be similar. On the assumption that nanoparticles attached to a lab-coat move out of the laboratory, a lab-coat to which nanocarbon black was attached was shaken and the concentration of the remaining particles on the lab-coat determined. The results confirmed that sufficient amounts of nanoparticles attached to the lab-coat could move from the laboratory to the office along the path of a researcher; thus, safety guidelines for the handling of lab-coat nanoparticles are required.

Crashworthiness analysis on existing RC parapets rehabilitated with UHPCC

  • Qiu, Jinkai;Wu, Xiang-guo;Hu, Qiong
    • Computers and Concrete
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    • v.19 no.1
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    • pp.87-98
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    • 2017
  • In recent year, the coat layer drops and the rebar rust of bridge parapets, which caused the structural performance degradation. In order to achieve the comprehensive rehabilitation, ultra high performance cementitious composites is proposed to existing RC parapet rehabilitation. The influence factors of UHPCC rehabilitation includes two parts, i.e., internal factors related with material, such as UHPCC layer thickness, corrosion ratio of rebars, fiber volume fraction, and external factors related with the load, such as impact speeds, impact angles, vehicle mass. The influence of the factors was analyzed in this paper based on the nonlinear finite element. The analysis results of the maximum dynamic deformation and the peak impact load of parapets revealed the influence of the internal factors and the external factors on anti-collision performance and degree degradation. This research may provide a reference for the comprehensive multifunctional rehabilitation of existing bridge parapets.

Morphological Characteristics and Proximate Compositions of the Recommended Soybean Varieties in Korea (장려품종 콩의 형태 및 성분특성)

  • Kim, Dong-Man;Jin, Jae-Soon;Kim, Kil-Hwan
    • Korean Journal of Food Science and Technology
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    • v.22 no.4
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    • pp.398-404
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    • 1990
  • This study was carried out for investigation of morphological characteristics and for analysis of the relationships between morphological indices and proximate compositions of the recommended soybean varieties in Korea. The weight of 100 seeds and weight percentages of seed coat and germ to the whole soybean of 19 varieties were ranged from 12.28 to 25.54g, from 6.02 to 8.29% and from 1.66 to 3.35%, respectively. The average long and short widths and length were $5.95{\sim}7.59mm,\;5.03{\sim}6.95mm\;and\;6.44{\sim}8.38mm$, respectively. The Hunter b values(yellowness) ranged from 15.9 to 21.7 for seed coat and from 17.7 to 23.1 for cotyledon. From the statistical analysis, a strong positive correlation(r>0.97) existed between total weight and cotyledon weight and long width, and between cotyledon weight and long width. Protein content had low correlations(r<0.48) with total weight, cotyledon weight, length and long width.

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Where to spot: individual identification of leopard cats (Prionailurus bengalensis euptilurus) in South Korea

  • Park, Heebok;Lim, Anya;Choi, Tae-Young;Baek, Seung-Yoon;Song, Eui-Geun;Park, Yung Chul
    • Journal of Ecology and Environment
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    • v.43 no.4
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    • pp.385-389
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    • 2019
  • Knowledge of abundance, or population size, is fundamental in wildlife conservation and management. Camera-trapping, in combination with capture-recapture methods, has been extensively applied to estimate abundance and density of individually identifiable animals due to the advantages of being non-invasive, effective to survey wide-ranging, elusive, or nocturnal species, operating in inhospitable environment, and taking low labor. We assessed the possibility of using coat patterns from images to identify an individual leopard cat (Prionailurus bengalensis), a Class II endangered species in South Korea. We analyzed leopard cat images taken from Digital Single-Lense Relfex camera (high resolution, 18Mpxl) and camera traps (low resolution, 3.1Mpxl) using HotSpotter, an image matching algorithm. HotSpotter accurately top-ranked an image of the same individual leopard cat with the reference leopard cat image 100% by matching facial and ventral parts. This confirms that facial and ventral fur patterns of the Amur leopard cat are good matching points to be used reliably to identify an individual. We anticipate that the study results will be useful to researchers interested in studying behavior or population parameter estimates of Amur leopard cats based on capture-recapture models.

Cytocompatible Coating of Individual Mammalian Cells with Tannic Acid-Zn Complex (타닌산-아연 복합체를 이용한 단일수준에서의 동물세포 코팅)

  • Lee, Juno
    • KSBB Journal
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    • v.32 no.2
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    • pp.160-167
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    • 2017
  • Coating of individual cells with organic or inorganic materials has drawn a great deal of attention, because it provides the cells with physicochemical durability, which would contribute to the development of bioreactors, biosensor, and lab-on-a-chip, as well as to the fundamental studies in single cell-based biology. Although many strategies have been developed for coating of microbial cells, limited methods are available to coat mammalian cells because most mammalian cells do not have a robust membrane or exoskeleton. Instead, they are enclosed in a lipid bilayer, which is fluidic and vulnerable to changes in its environments. It is more difficult to treat mammalian cells in vitro than microbial cells because the surfaces of mammalian cells are not protected or reinforced by a tough coat. In this work, we report a cytocompatible and degradable nanocoat for mammalian cells. Three types of mammalian cells (HeLa cells, NIH 3T3 fibroblasts, and Jurkat T cells) were individually coated within metal-polyphenol. To maintain the viability of the mammalian cells, we performed the whole processes under strictly physiological culture conditions, and carefully selected nontoxic materials.

Systematic application of seed morphology In Korean Orobanchaceae (한국산 열당과 종자 형태의 분류학적 적용)

  • An, Beom-Cheol;Hong, Suk-Pyo
    • Korean Journal of Plant Taxonomy
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    • v.33 no.4
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    • pp.411-420
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    • 2003
  • To examine the seed morphology in Korean Orobanchaceae, seeds of 5 species including 1 variety in five genera and one related taxon, Boschniakia himalaica Hook. f. & Thomson in Hook. f. from the Himalaya (7 taxa in total) were investigated by scanning electron microscopy (SEM). Two different seed types are recognized depending on three characters (e.g, .the seed size, seed coat structure and seed number per fruit). - Type I: size less than 1mm, seed coat structure reticulate and seed number per fruit more than ca. 1,000; Type II: size more than 1 mm, exotesta irregularly crested and seed number per fruit less than 1,000 (Lathraea japonicu Miq.). In Type I, three subtypes of exotesta internal surface pattern can also be classified; (1) Subtype Ia: reticulate - include irregularly striate (Ia') and deeply reticulate (Ia"), (2) Subtype Ib: foveolate, (3) Subtype Ic: smooth. Systematics application of the seed morphological data for the Korean Orobanchaceae is also briefly discussed.

Quantitative In-line NIR measurements of papers

  • Schmidt, Angela;Weiler, Helmut
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1285-1285
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    • 2001
  • For NIR measurements of papers normally diffuse reflectance accessories are used which can provide a large sampling area. The in-line process control FT-NIR spectrometer MATRIX-E enables the contactless measurement of paper samples of low silicone coat weights on label-stocks in a paper converting factory. For this study concentrations of silicone between 0 and 2 g/$m^2$ on various paper substrates were included in a quantitative method. The aim was to achieve an absolute value for the deviation from the target value of 1 g/$m^2$ during continuous movement of the paper with velocities around 400 numinute. Influences from the uncoated paper type due to supplier, color, opacity, area densities, pre-coating as well as different compounds of the agent silicone were investigated and it was found that all these papers can be represented in one PLS-model. Especially the fact that silicone as an element is present in clay coated papers is of no consequence to the measurements with MATRIX-E. Moreover during in-line installations the variation of the moisture contents in the moving paper due to variable machine velocities as well as the reflecting material of the cylinder have to be considered. It is shown that the result of the in-line calibration has the same prediction ability compared to lab scale results(Root Mean Square Error of Cross-Validation RMSECV = 0.034 g/$m^2$).

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