• Culinary science and hospitality research
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• v.13 no.1 s.32
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• pp.55-61
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• 2007

This study was carried out to evaluate the growth characteristics of Lactobacillus acidophilus KCCM 32820 and Propionibacterium freudenreichii KCCM 31227 and the production of propionic and acetic acids in 5% and 10% whey broth by mixed culture of L. acidophilus KCCM 32820 and P. freudenreichii KCCM 31227. Exponential phase of L. acidophilus KCCM 32820 was in the range of $6\sim12$ hrs and P. freudenreichii KCCM 31227 was in the range of $36\sim108$ hrs. In the mixed culture, production of propionic acid was shown to be greater value in the 10% whey broth than in the 5% whey broth and to be greater value in the low temperature for a long time than in sterilization by autoclave. Maximum production of propionic acid was 8.88 mg/mL in the 10% whey broth fermented at 120hrs. Production of acetic acid was revealed to be greater value in the 10% whey broth than in the 5% whey broth. The production quantity ratio of propionic acid to acetic acid was shown between $2:1\sim3:1$ during the fermentation process.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.294-302
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• 2018

This study was conducted to compare anti-inflammatory effect of Robinia pseudoacacia L. using different extraction methods (water extraction, ethanol extraction and high temperature extraction). We investigated anti-inflammatory effect of Robinia pseudoacacia L. extract (RP1, water extract; RP2, ethanol extract; RP3, high temperature extract) on lipopolysaccharide (LPS)-stimulated inflammation using Raw 264.7 cell. Cells were treated with various concentrations (12.5, 25, 50, 100 or $200{\mu}g/m{\ell}$) of water extract, ethanol extract and high temperature extract. Cytotoxicity was not observed on Raw 264.7 cells, LPS-stimulated production of NO (nitric oxide), $PGE_2$ (prostaglandin $E_2$) and cytokines ($TNF-{\alpha}$, IL-6 and $IL-1{\beta}$) was reduced by RP3 treatment more than RP1 and RP2. In conclusion, these results indicated that inflammation on Raw 264.7 cells was improved by RP3. Treatment of RP3 could be used to natural medicine for improving inflammatory response. However, further experiment is required to observe how the high temperature extraction at $500^{\circ}C$ for 48 h influences on alteration of active ingredient in Robinia pseudoacacia L., and conducts the inflammation signal pathway on Raw 264.7 cells.

• Development and Reproduction
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• v.16 no.2
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• pp.137-143
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• 2012

The main objective of this study is to investigate the effects of ovulation induction by treating HCG, LHRHa, GnRHa, ovaprim and pimozidein in long snout bullhead, L. longriostris. All hormons were injected into the muscles of back. Concentration of LHRHa to injection were 20, 50, 100, 150 and 200 ${\mu}g/kg$ and same concentration of LHRHa was injected after 24 hour. The ovulation induction rate was 100% and fertilization and hatching rates were 68.4, 45.2, 58.4 and 33.6% in 50 and 100 ${\mu}g/kg$. The times to ovulation were between 28 and 44 h. HCG was injected in long snout bullhead at 5,000, 10,000, 15,000, 20,000 and 25,000 IU/kg. The ovulation induction rate was 50% in 15,000 and 20,000 IU/kg. Fertlilzation and hatching rates were 55.2, 45.6, 52.8 and 44.8%. Ovulation time was between 72~80 h. HCG concentration of 500, 1,000, 2,000 and 4,000 IU/kg were injected with $50{\mu}g$ of LHRHa. Ovulation and hatching rates in 2,000 IU/kg were 75 and 35%. Ovulation time was 28~48 h. Ovaprim of 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mL/kg were injected to the abdominal cavity. The ovulation induction rate was highest at 2.5 mL/kg to 50% and ovulation time was between 66~86 h. LHRHa concentration of 50, 100, 200, 300 and 400 ${\mu}g/kg$ was injected with pimozide (1,000 ${\mu}g/kg$). Ovulation induction rate was 87% at 100 and 200 IU/kg with pimozide. Ovulation time was between 66~86 h.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.30-41
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• 2011

The influences of the agitation as well as the addition of medium during culture on the production of embryos were invested in isolated microspore culture of hot pepper (Capsicum annuum L.). When the culture medium was added during initial liquid culture step of liquid-double layer culture, the embryo yield and quality greatly increased. The most effective time point for medium addition was 5 days after the culture commenced. On the other hand, the effect of medium addition at later double layer culture step in liquid-double layer culture on the embryo production was less compared to that of medium addition during the initial liquid culture step. Agitating the culture for 1 week during later double layer culture step in liquid-double layer culture effectively increased the production of normal cotyledonary embryos. In the case of liquid culture, agitating the culture for 1 week from 7 days after the culture commenced was also effective for embryo development. However, when the total agitation time was longer (2 to 3 weeks) during liquid-double layer culture or liquid culture, the embryos developed abnormally in both cases. The normal cotyledonary embryos obtained in this study successfully developed to plants when transferred to regeneration media. These regenerated plants were either diploid or haploid, and there was a difference in the number of chloroplasts between guard cells of diploid and haploid. These results can be used as an important data for developing an efficient microspore culture system with high quality embryo production in hot pepper.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.8
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• pp.1278-1284
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• 2003

This study was carried out to investigate the optimum conditions for making “sponge cake” with addition of ingredient, laver powder, and the quality characteristics of a new sponge cake. The moisture contents of sponge cake with 2, 4, 6% laver powder did not change but with 8 and 10%, the moisture contents increased to 28.89% and 30.69%, respectively. While specific gravity increased to 0.674 (control 0.493) when 10% laver powder was added, but volume was decreased. Most abundant mineral was Ca, followed by Na, Fe, Mn, Cu and Zn. The crust color degree of sponge cake with laver powder showed low marks on L, a, b. The crumb color degree showed low marks on L, b while “a” degree redness indicated highest marks with 4% laver powder. When it was seen under the microscope, the air cell numbers were decreased but the cell size became bigger. More free amino acid was contained when the laver powder was added. L-glutamic acid, L-alanine, L-aspartic acid and L-leucine were the major free amino acids. Hardness, springiness, cohesiveness, gumminess, chewiness and resilience degree of sponge cake with addition of laver power were higher than those of control. The sensory evaluation indicated that addition of 2% laver powder enhanced most mouth feeling, appearance, hardness, moistness, flavor and overall acceptability.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.88-92
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• 1994

This study was carried out to investigate the effects of lime(0, 250, 500 and 1,000 kg/lOa) and phosphate (0, 17 and 34 kg/lOa) applications on growth and nitrogen fixation of alfalfa (Medicago sativa L.). The phosphate applications improved(p<0.05) shoot, root and root nodule dry weight of Alfalfa and acetylene reduction activity of alfalfa with increasing levels of phosphate in the soil pH 6.2 at 7 and 12 weeks(ear1y bloom) after sowing, but these were not influnced with lime applications. Total nitrogen content of each part of alfalfa was increased with lime application, but was not significantly different by levels of lime application at 7 weeks after sowing. Application of phosphate did not affect total nitrogen content of each part of alfalfa. Total nitrogen contents of each part of alfalfa were not significantly different between lime and non-lime application at 12 weeks(ear1y bloom) after sowing but were decreased(p<0.05) with phosphate application(p< 0.05). These results suggest that lime(250 kg/lOa) and phosphate(34 kg/lOa) applications are effective for the growth and the nitrogen fixation of alfalfa at the soil pH 6.20.

• Journal of Sensor Science and Technology
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• v.1 no.2
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• pp.125-130
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• 1992

A resistive-type humidity sensors have been fabricated using cross-linked PMMA thin film as sensing material and their humidity characteristics have been investigated. The sensor coated of the cross-linked PMMA with PVA exhibited largely variation of resistance by increase of relative humidity and less than 3% of hysteresis. Furthermore, the fabricated sensor exhibited superior long-term stability. The response time of the PMMA humidity sensor was about 7 min. for adsorption and about 5 min. for desorption respectively.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.79-79
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• 2011

도시지역에서 강우발생 시 우수는 대부분 도로를 통해 이동하고 배수된다. 도로의 배수시설은 도로면의 안전을 확보하기 위한 목적뿐만 아니라 도로 이외의 지역에 흐르는 유출수를 배수시키는 기능도 포함하고 있다. 현재 우리나라의 도로배수는 빗물받이와 횡유입부를 통해 대부분 이루어지고 있다. 이러한 배수시설이 제 기능을 충분히 발휘하지 못하면 노면수가 정체되고, 노면수가 인근 주택가로 유입되어 침수피해를 가중시키는 경우도 있고, 교통의 원활한 흐름을 저해하게 된다. 도로배수시설의 설계 시 설계빈도에 상위하도록 충분한 여유를 두고 설치하더라도 부유잡물 등에 의해 그 효율이 저하될 수도 있다. 또한 배수시설을 통해 배수되는 초기 유출수는 많은 비점오염원을 포함하고 있고, 이러한 오염원이 하천으로 유입되어 하천수 및 저수지를 오염시킬 수도 있다. 본 연구에서는 배수시설 중 빗물받이의 효율개선과 비점오염원을 처리할 수 있는 비노출배수로에 대하여 수리실험을 실시하고 효과를 검토하였다. 이를 위해 폭 2.4 m, 길이 10.0 m의 실험도로를 제작하였고, 유입유량을 증가시켜가며 배수효율을 확인하였다. 또한 흙탕물을 유입시켜 비노출 배수로를 통해 배수시킨 후 유입수와 유출수의 부유물질, BOD, $COD_{Mn}$, TN, TP의 량을 측정하고 변화를 확인하였다. 이때 비점오염원처리를 위한 여과재료로는 제오라이트를 이용하였다. 실험결과 실험도로의 측면에 비노출 배수로를 설치하였을 경우 도로위에 수심이 거의 발생하지 않으면서 배출할 수 있는 최대 유량은 2.29 l/s였고, 서울시를 기준으로 설계빈도 10년에 대하여 본 연구의 실험도로 규격에서의 유출량이 1.09 l/s임을 고려할 때 10년 빈도 강우강도 발생 시 비노출 배수로가 충분한 여유를 가지고 배수 시킬 수 있을 것으로 판단된다. 본 실험도로의 규격에서 2.29 l/s의 유출량이 발생하기 위한 강우강도는 서울시를 기준으로 100년 빈도에 상위하는 강우강도로 비노출 배수로에 막힘이 없는 경우 100년 빈도의 강우시에도 노면수 배출이 가능하였다. 쓰레기와 모래와 같은 부유잡물이 배수로를 막고 있다 하더라고 배수효율 저하는 크게 발생하지 않았다. 또한 BOD와 $COD_{Mn}$의 농도는 유입수와 비교하여 30%이상의 제거 효율을 보였다. 특히 부유물질의 경우 제거효율이 50 %인 것으로 검토되어 본 연구의 비노출 배수로가 초기우수의 오염원제거에 효과가 있을 것으로 판단된다.

• Journal of Wetlands Research
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• v.11 no.1
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• pp.91-97
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• 2009

The fowls manure is using as fertilizers for farmland because of enough nutrients. However, excess nutrients can be washed off during a storm and affected on nearby waterbodies. In this paper, the runoff characteristics from farmland were studied to determine the washoff mass. A lab-scale reactor was designed to estimate the surface runoff and infiltration rates according to the rainfall intensity. Surface runoff water did not occur at 10mm/hr rainfall intensity, but some runoff occurred at 20mm/hr rainfall intensity. At 32.4mm/hr rainfall intensity, it shows the highest pollutant concentrations such as 686mg/L for $BOD_5$ and at 630mg/L $COD_{Mn}$. The pollutant as based on fowls manure compost was highly washed-off by subsurface water at 32.4mm/hr rainfall intensity, however the concentration was largely decreased at 43.2mm/hr rainfall intensity. The summary of the results is that the highest wash-off concentrations value shows at 32.4mm/hr rainfall intensity for $BOD_5$ and at 67.1mm/hr for T-N and T-P.

• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.347-351
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• 1998

Plant regeneration via organogenesis from leaf and stipule explants of micropropagated shoots of strawberry (Fragaria $\times$ ananassa cv. Suhong) was achieved. Leaf and stipule explants were detached from shoot-tip cultured shoots and cultured on MS medium with various combinations of BA and NAA under light or dark condition. Shoot regeneration from leaf explant was observed after 3 weeks in culture and was good at the high ratio of BA and NAA among various combination treatments. The highest shoot regeneration frequency from leaf explants was obtained with 1.0 mg/L BA and 0.1 mg/L NAA, in which 31.1% shoot regeneration frequency(1.7 shoots per leaf explant) was yielded. In case of stipule explants, shoot regeneration was largely affected by plant growth regulators during incubation under dark condition for initial 4 weeks but not under continuous light condition. The combination treatment with 2.0 mg/L BA and 0.1 mg/L NAA showed the most excellent shoot regeneration from stipule explants, where 44.4% regeneration frequency(4.0 shoots per explants) obtained. Regenerated shoots were rooted on MS medium with 0.1 mg/L NAA after shoot elongation, and the plantlets regenerated were transferred to soil mixtures with vermiculite and perlite for acclimation.