• Korean Journal of Veterinary Research
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• v.32 no.4
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• pp.497-510
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• 1992

This study was attempted to comparative investigate the types and regional distribution of the endocrine cells in several vertebrates immunohistochemically using seven antisera. From carp pancreas could be observed 4 types which are insulin-, glucagon-, som- and BPP-immunoreactive cells. Insulin-immunoreactive cells were mainly distributed at the periphery and a few cells occupied the central region of the islets. Glucagon-immunoreactive cells were distributed at the periphery of the islets, and som - and BPP-immunoreactive cells were located at the central region. From frog pancreas could be observed 4 types which are insulin-, glucagon-, som- and BPP-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the islets. Som-immunoreactive cells were distributed at the periphery of the islets, and glucagon- and BPP-immunoreactive cells were found as single cell or as small groups located between the pancreatic acini. From snake pancreas could be observed 3 types which are insulin-, glucagon- and som -immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the small islets, and they also were scattered at the periphery of the large islets. Glucagon-immunoreactive cells were distributed at the periphery of the islets, whereas som-immunoreactive cells were occupied the central region. From Ogolgae pancreas could be observed 4 types which are insulin-, glucagon-, som-and BPP-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the small islets, but at the periphery of the large one. Glucagon- immunoreactive cells were distributed at the periphery of the small islets and in the large islets showed scattering entired. Som-immunoreactive cells were distributed at the periphery of the small islets and in the large islets were located at the central region. A small numbers of BPP-immunoreactive cells were located at the periphery of the small islets and the exocrine regions. From the pancreas of the Korean native goat could be observed 6 types which are insulin-, glucagon-, som-, BPP-, 5-HT- and porcine-CG-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the islets. Som-immunoreactive cells were located at the periphery of the islets, but a tew were scattered at the central region of islets and in the epithelium of the secretory duct. Glucagon-, BPP-, 5-HT- and porcine CG-immunoreactive cells were distributed at the periphery of the islets. These findings indicated that the regional distribution patterns and cell types of pancreatic endocrine cells in vertebrates varies considerably among phylogenetically different vertebrates.

• Korean Journal of Veterinary Research
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• v.14 no.2
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• pp.135-150
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• 1974

한국재내산양(韓國在來山羊) 11마리의 전지근(前肢筋)을 절개하여 관찰하였던 바 다음과 같은 결과를 얻었다. 1. 한국재내산양(韓國在來山羊)의 전지(前肢)에서는 다음과 같은 근(筋)들을 관찰할 수 있었다. 승모근(僧帽筋) M. trapezus, 릉형근(菱形筋) M. rhomboideus, 완두근(腕頭筋) M. brachiocephalicus, 쇄골하근(鎖骨下筋) M. subclavius, 견갑횡구근(肩甲橫究筋) M. omotransv-ersarius, 란배근(瀾背筋) M. latissimus dorsi, 천흉근(淺胸筋) M. pectoralis guperficialis, 탐흉근(探胸筋) M. pectorlis profundus, 복거근(腹鋸筋) M. serratus ventralis, 삼각근(三角筋) M. deltoideus, 극하근(棘下筋) M. infraspinatus, 극상근(棘上筋) M. supraspinatus, 견갑하근(肩甲下筋) M. subscapularis, 대원근(大圓筋) M. teres major, 소원근(小圓筋) M. theres minor, 전완근막장근(前腕筋膜張筋) M. tensor fascia antebrachii, 삼두완근(三頭腕筋) M. triceps brachii, 주근 M. anconeus, 이두완근(二頭腕筋) M. biceps brachii, 상완근(上腕筋) M. brachialis, 조훼완근(鳥喙腕筋) M. coracobrachialis, 요완신근(橈腕伸筋) M. extensor carpi radialis, 고유제삼지신근(固有第三指伸筋) M. extensor digiti tertii proprius, 총지신근(總指伸筋) M. extensor digitorum cemmunis 고유제사지신근(固有第四指伸筋) M. extensor digiti quartii proprius, 척완신근(尺腕伸筋) M. extensor carpi ulnaris, 장모지외전근(長母指外轉筋) M. abductor pollicis longus, 척완굴근(尺腕屈筋) M. flexor carpi ulnaris, 요완굴근(橈腕屈筋) M. flexor carpi radialis, 원회내근(圓回內筋) M. pronator teres, 천지굴근(淺指屈筋) M. flexor digitorum suprficialis, 탐지굴근(探指屈筋) M. flexor digitorum profundus, 골간근(骨間筋) M. interosseus medius. 2. 천흉근(淺胸筋)과 심흉근(深胸筋)은 각각 전부(前部)와 후부(後部)로 명확히 분리(分離)되어있으며 특히 심흉근(深胸筋)의 전부(前部)와 후부(後部)는 서로 떨어져서 기시(起始)를 하고있어 그 사이에는 흉골(胸骨)이 노출되어 있었다. 3. 쇄골하근(鎖骨下筋)은 전예(全例)에서 관찰할 수 있었다. 4. 조탁흉근(鳥啄胸筋)은 소나 양에 비하여 매우 발달하였으며 특히 3예(例)에서는 더욱 발달하여 3개의 부분(部分)으로 되어있어 상완골 내측면 거의 전체를 덮고 있었다. 5. 주근, 소원근(小圓筋) 등 소동물(小動物)에서는 작은 근(筋)들이 매우 발달하였으나 장모지외전근(長母指外轉筋)은 엷고 작았다. 6. 반추류(反芻類)에서 가끔 볼 수 있는 M. extensor pollicis는 관찰할 수 없었다.

• Korean Journal of Agricultural Science
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• v.32 no.2
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• pp.197-203
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• 2005

This study was conducted to investigate the effects of the addition of activated charcoal (AC) and oak charcoal on the ruminal fermentation characteristics, nutrient utilization in Korean native goats. Compared to reference diet, digestibility of dry matter, and crude protein in AC and charcoal diets tended to incraese. However, any tendency in ruminal degradation of crude fat was not observed. Ruminal degradation of NDF in AC diets tended to be more increased than that in non-AC diet. Although it was not significant, ADF tended to be increased in AC and charcoal diets. AC and charcoal did not affect the ruminal pH and ammonia-N. Concentration of total VFA and butyrate tended to be increased by adding AC and charcoal(p<0.05). Although it was not significant, acetate/propionate ratio tended to decreased in AC and charcoal diets. Although there appeared some beneficial effects in adding AC and charcoal to ruminant diets in this study, more works could be needed with AC before we can make clear conclusion on use of AC and charcoal in the ruminant diets.

• Journal of Animal Science and Technology
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• v.48 no.3
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• pp.415-424
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• 2006

The purpose of this study was to investigate the fungal growth and enzyme production under different carbohydrate substrate conditions. The anaerobic fungus Neocallimastix sp. NLRI-3 isolated from the rumen of Korean native goat was incubated with different carbohydrate media containing 0.2% of glucose, starch, rice straw, filter paper, carboxymethyl cellulose(CMC), Sigmacell cellulose, xylan or xylose, respectively. The culture head gas production was the highest in the culture of filter paper medium, and the lowest in the culture of CMC medium at 96h incubation (P<0.05). The fungal zoospore production reached peak at 72h incubation, and its number was the highest in rice straw medium among the treatments (P<0.05). At 96h incubation, carboxymethyl cellulase(CMCase) activity was the highest in the culture of filter paper medium and the lowest in the culture of starch medium (P<0.05). While xylanase activity was the highest in the culture of rice straw medium and the lowest in the culture of xylose medium(P<0.05) at 72h incubation. There were no differences in culture supernatant protein expression among the treatments. However, the patterns of enzyme expression were different among the treatments with zymogram analysis. Six CMCases and 4 xylanase were detected from the results of zymogram analysis. Therefore the present study indicating that the fungal enzyme expression could be stimulated with insoluble substrates in the culture medium.

• Journal of Chest Surgery
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• v.37 no.4
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• pp.297-306
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• 2004

Current artificial heart valves have several disadvantages, such as thromboembolism, limited durability, infection, and inability to grow. The solution to these problems would be to develop a tissue-engineered heart valves containing autologous cells. The aim of this study was to optimize the protocol to obtain a porcine acellular matrix and seed goat autologous endothelial cells on it, and to evaluate the biological responses of xenograft and xeno-autograft heart valves in goats. Material and Method: Fresh porcine pulmonic valves were treated with one method among 3 representative decellularization protocols (Triton-X, freeze-thawing, and NaCl-SDS). Goat venous endothelial cells were isolated and seeded onto the acellularized xenograft leaflets. Microscopic examinations were done to select the most effective method of decellularizing xenogeneic cells and seeding autologous endothelial cells. Two pulmonic valve leaflets of. 6 goats were replaced by acellularized porcine leaflets with or without seeding autologous endothelial cells while on cardiopulmonary bypass. Goats were sacrificed electively at 6 hours, 1 day, 1 week, 1 month, 3 months, and 6. months after operation. Morphologic examinations were done to see the biological responses of replaced valve leaflets. Result: The microscopic examinations showed that porcine cells were almost completely removed in the leaflets treated with NaCl-SDS. The seeded endothelial cells were more evenly preserved in NaCl-SDS treatment. All 6 goats survived the operation without complications. The xeno- autografts and xenografts showed the appearance, the remodeling process, and the cellular functions of myofibroblasts, 1 day, 1 month, and 3 months after operation, respectively. They were compatible with the native pulmonary leaflet (control group) except for the increased cellularity at 6 months. The xenografts revealed the new endothelial cell lining at that time. Conclusion: Treatment with NaCl-SDS was most effective in obtaining decellularized xenografts and facilitate seeding autologous endothelial cells. The xenografts and xeno-autografts were repopulated with myofibroblasts and endothelial cells in situ serially. Both of grafts served as a matrix for a tissue engineered heart valve and developed into autologous tissue for 6 months.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.11
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• pp.1562-1570
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• 2014

The effects of Lactobacillus mucosae (L. mucosae), a potential direct fed microbial previously isolated from the rumen of Korean native goat, on the rumen fermentation profile of brewers grain were evaluated. Fermentation was conducted in serum bottles each containing 1% dry matter (DM) of the test substrate and either no L. mucosae (control), 1% 24 h broth culture of L. mucosae (T1), or 1% inoculation with the cell-free culture supernatant (T2). Each serum bottle was filled anaerobically with 100 mL of buffered rumen fluid and sealed prior to incubation for 0, 6, 12, 24, and 48 h from which fermentation parameters were monitored and the microbial diversity was evaluated. The results revealed that T1 had higher total gas production (65.00 mL) than the control (61.33 mL) and T2 (62.00 mL) (p<0.05) at 48 h. Consequently, T1 had significantly lower pH values (p<0.05) than the other groups at 48 h. Ammonia nitrogen ($NH_3$-N), individual and total volatile fatty acids (VFA) concentration and acetate:propionate ratio were higher in T1 and T2 than the control, but T1 and T2 were comparable for these parameters. Total methane ($CH_4$) production and carbon dioxide ($CO_2$) were highest in T1. The percent DM and organic matter digestibilities were comparable between all groups at all times of incubation. The total bacterial population was significantly higher in T1 (p<0.05) at 24 h, but then decreased to levels comparable to the control and T2 at 48 h. The denaturing gradient gel electrophoresis profile of the total bacterial 16s rRNA showed higher similarity between T1 and T2 at 24 h and between the control and T1 at 48 h. Overall, these results suggest that addition of L. mucosae and cell-free supernatant during the in vitro fermentation of dried brewers grain increases the VFA production, but has no effect on digestibility. The addition of L. mucosae can also increase the total bacterial population, but has no significant effect on the total microbial diversity. However, inoculation of the bacterium may increase $CH_4$ and $CO_2$ in vitro.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.2
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• pp.198-205
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• 2011

This study was conducted to investigate the effect of feed types on Ochratoxin A (OTA) degradation by Korean native goats. Rumen fluid from canulated goats fed whole roughage or 50% roughage served as a source of micro-organisms. Experiments were undertaken i) to investigate OTA degradation ability in a $2{\times}4$ factorial arrangement with different feed types (100% roughage vs. 50% roughage) and rumen fluid fractions (whole rumen fluid, cells, autoclaved rumen fluid and supernatant) supplemented with OTA ii) to evaluate OTA degradation by the rumen fluid of goats fed two different diets at different time points (0, 3, 6, 9 and 12 h) of feeding iii) to isolate potential rumen microorganisms and iv) to identify elements responsible for OTA degradation. Rumen fluid from goats fed 100% roughage had higher (p<0.05) OTA degradability than 50% roughage diets. OTA degradation based on rumen fluid collection times showed that rumen fluid at 0 h showed significantly higher (p<0.05) degradability. Carboxypeptidase A (CPA) enzyme has been reported to be responsible for OTA degradation. Thus, using real time PCR, primers designed to target the CPA gene from Bacillus licheniformis could be amplified using genomic DNA from rumen fluid of goats and sequenced, thus enabling evaluation of the Bacillus population under different feeding condition and times. Our findings showed that the Bacillus population was significantly higher (p<0.05) before feeding (0 h) in animals which were fed a whole roughage diet, giving indirect evidence of OTA degradation being influenced by Bacillus sps. Thus, it can be concluded that OTA degradability is influenced by feed, feeding time and Bacillus licheniformis population.

• Korean Journal of Agricultural Science
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• v.12 no.2
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• pp.260-271
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• 1985

A study was carried out to improve the feeding practice of Korean native goats. Ten goats were used in this study to obtain basic information needed for the determination of energy and nitrogen requirements for maintenance and growth. The results obtained were as follows. 1. The daily gain of male and female goats were $80{\pm}13.9g(\bar{x}{\pm}S.E.)$ and $41{\pm}8.9g$, respectively. 2. The amounts of orchardgrass hay and formula feed consumption by goats were 0.60-0.75% and 1.80-2.80% of their body weights, respectively, when fed ad libitum. The daily consumption of orchardgrass hay by goats when fed alone was 1.98-3.16% of their body weights. 3. The amounts of metaholizable energy needed for daily maintenance were 61.6, 65.0 and $72.3Kcal/BW_{kg}{^.75}$ at the live weights of 30, 21 and 12kg, respectively. 4. The daily nitrogen requirements for maintenance were 7.07, 4.67 and $3.04g/BW_{kg}{^.75}$ at the live weights of 30, 21 and kg, respectively. 5. Daily energy retention was 138 kcal/head and nitrogen retention rate was 13.3-22.5%.

• Journal of Embryo Transfer
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• v.19 no.2
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• pp.81-87
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• 2004

This study was designed to determine whether repeated superovulation is beneficial for recovery and quality of oocytes in Korean native goats. Seventy-six mature goats, maintained in a pen under natural day length and fed hay ad libitum, were pretreated with progestagen impregnated CIDR for 10 days and then the goats were divided into two groups. One group of the goats received a single intramuscular injection of 1,000 IU PMSG on Day 8 of CIDR insertion. The other group of the goats received twice daily intramuscular injections of a total of 70 mg FSH for 3 days from Day 8 of CIDR. All the gonadotropin treated goats were injected with 10 mg $PGF2{\alpha}on$ Day 8 and 400 IU hCG in the afternoon on Day 10. For oocyte recovery, donor goats were fasted 24 h before operation. Anesthesia was induced by intravenous injection of 2% xylazine(0.2 mg/kg body weight) and ketamin(11 mg/kg body weight). In vivo oocytes were recovered by follicle aspiration or oviduct flushing at 35 to 40 hours after hCG injection through mid-ventral incision. The mean number of CL and oocytes recovered and recovery rate of oocytes by oviduct flushing were greater(P<0.05) in the first treatment than those in the second treatment. Contrary to our assumption, PMSG treatment significantly (P<0.05) increased the number of CL formed and recovery rate of oocytes compared to FSH. However, the same effect was not observed in recovery of follicular oocytes. There was no significant difference in oocyte quality between FSH and PMSG or first and second treatments. The present results indicate that repeated superovulation and repeated use of donor animals may be inefficient for obtaining oocytes in good qualities.

• Korean Journal of Agricultural Science
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• v.10 no.2
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• pp.212-220
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• 1983

In order to improve the quality of sweet potato silage by adding some additives. sweet potatos (80%) were ensiled with one (20%) of the followings ; wheat bran, layer waste, swine waste or cow waste. The chemical composition, pH and acid contents of silages were determined. In addition, the silage intakes by Korean native goats were measured to estimate the palatability. The results were summarized as follows. 1. The pH of silages were decreased from the criginal 6.95 to 4.00 for WBAS (wheat bran added silage), to 4.50 for LWAS (layer waste added silage), to 4.40 for SWAS (swine waste added silage) and to 4. 10 for CWAS (cow waste added silage) after 40 days ensiling. 2. The contents of total acid and latic acid were 3.50% and 3. 32% for WBAS, 9.55% and 9.23% for LWAS, 8.51% and 8.50% for SWAS and 0.93% and 0.90% for CWAS, respectively. Therefore, good results for acid content were abtained from WBAS, LWAS and SWAS. 3. The bacterial counts were $2.6{\times}10^7/g$ for WBAS, $2.8{\times}10^7/g$ for LWAS, $2.6{\times}10^7/g$ for SWAS and $1.9{\times}10^7/g$ for CWAS. The number of lactic acid bacteria were $4.0{\times}10^7/g$ for WBAS, $5.5{\times}10^7/g$ for LWAS, 4.6{\times}10^7/g$ for SWAS, and $4.2{\times}10^7/g$ for CWAS. 4. The content of crude protein was highest in LWAS, that of crude fat was highest in SWAS and that of crude fiber was highest in CWAS. The contents of crude protein and crude fat in the silages were slightly increased while the moisture contents decreased as the fermentation was progressed. 5. The silage intakes by Korean native goat were slightly lower for animal-wasie-added silages than wheat-bran-added silage. Among the animal-added silage, the intakes of LWAS and SWAS were slightly higher than CWAS.