• Title/Summary/Keyword: kimchi fermentation

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Isolation and characterization of bacteriophage infecting Lactobacillus plantarum KCCM 12116

  • Oh, Jiyoung;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.53 no.3
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    • pp.348-355
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    • 2021
  • Bacteriophages (phages) are known determinants of kimchi microbial ecology. Lactobacillus plantarum is related to kimchi over-acidification during the late stages of kimchi fermentation. A phage infecting Lac. plantarum was isolated from kimchi and characterized. The phage population for kimchi in a market was 2.3 log particles/mL, which corresponded to 32% of the bacterial population on a log scale. The isolated phage was designated as ΦLP12116. ΦLP12116 which belonged to the Siphoviridae family and has a very narrow host range, infecting only Lac. plantarum. The phage was stable at a lactic acid concentration of 1.0% and pH 4.0 at 4℃, indicating that it could survive in kimchi. In the kimchi extract broth treated by the phage, the growth of Lac. plantarum KCCM 12116 was inhibited by 2.2 log CFU/mL compared to the growth in non-phage-treated broth. Therefore, this study suggests that the growth of Lac. plantarum, which is known as an acid-producing strain during late fermentation in kimchi, may be controlled using the phage.

Effect of Low Temperature on the Qualities of Long-term Fermented Kimchi (Korean Pickled Cabbage) (저온이 묵은지의 발효에 미치는 영향)

  • Ji, Seol-Hee;Han, Woo-Cheul;Lee, Jae-Cheol;Cheong, Chul;Kang, Soon-Ah;Lee, Je-Hyuk;Jang, Ki-Hyo
    • Food Science and Preservation
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    • v.16 no.6
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    • pp.804-809
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    • 2009
  • Recently, many Koreans have started to consume aged kimchi fermented long-term at low temperatures. In the present study, the effect of low temperature ($5^{\circ}C$) on pH, titratable acidity (TA), organic acid level, viable microbial cell count, amino acid concentration, and polygalacturonase activity (PG) during long-term fermentation (46 weeks) of kimchi, were evaluated. After 10 weeks of fermentation, kimchi had a pH of 4.1 and a TA of 1.0%, respectively after 46 weeks fermentation, these values were 3.9 and 1.3%, respectively. Lactic acid, the ratio of lactic acid to acetic acid, and the ratio of Lactobacillus species/Leuconostoc species in kimchi increased as fermentation progressed from 10 weeks to 46 weeks. However, total viable cell counts of aerobic bacteria, yeasts, Lactobacillus species, and Leuconostoc species, free amino acid levels, and PG decreased as the fermentation period was extended from 10 weeks to 46 weeks.

Chemical Characteristics of Taurine Added Kimchi during Fermentation at Low Temperature (타우린 첨가 김치의 저온 저장 중 화학적 특성)

  • Yim, Seoung-Been;Kim, Mi-Sook;Kim, Eun-Kyung;Ko, Jae-Youn;Jeong, Yoon-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.12
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    • pp.1814-1818
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    • 2010
  • The present study was carried out to elucidate the changes in the chemical characteristics of taurine added Kimchi during fermentation for 46 days at $6^{\circ}C$. Chinese cabbage was brined in a 10% salt solution for the control Kimchi and in a 10% salt solution containing 5% taurine for taurine added Kimchi (Taurine I, II, and III). One and three percent (w/w, based on Chinese cabbage) of taurine were added to make Taurine II and Taurine III, respectively. Reducing sugar and vitamin C contents for all the samples decreased after the 46-day fermentation. Taurine was not detected in the control, and the taurine contents, from the largest to the smallest, during the fermentation period were Taurine III, Taurine II and Taurine I. Amino nitrogen contents in all the samples studied proportionally increased during the 46-day fermentation. It is suggested that taurine does not affect the chemical characteristics of Kimchi during fermentation at low temperature ($6^{\circ}C$).

Evaluation of Physiochemical Properties and Fermentation Qualities of Kimchi Supplemented with Yacon (야콘 첨가 김치의 이화학적 특성과 발효 품질 평가)

  • Lee, Dong-Ho;Ji, Seol-Hee;Han, Woo-Cheul;Lee, Jae-Cheol;Kang, Soon-Ah;Jang, Ki-Hyo
    • Journal of the East Asian Society of Dietary Life
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    • v.22 no.3
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    • pp.408-413
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    • 2012
  • This study aimed to evaluate the physiochemical properties and fermentation qualities of kimchi supplemented with yacon (Smallanthus sonchifolius) during fermentation. Kimchi supplemented with 0%, 0.1%, 0.5%, 1%, and 5% yacon were investigated. As fermentation proceeded, pH decreased from 5.5~5.7 on the first day to 4.6~4.8 on day 21, whilst total titratable acidity (TTA), lactate levels, and number of microbes increased. The kimchi with 5% yacon showed a higher production of lactate concentration at 7 days fermentation than other groups, which is consistent with the observed high numbers of viable Leuconostoc sp. cells at 7 days fermentation. These observations demonstrate that 5% yacon stimulates the growth of kimchi microbes, particularly of Leuconostoc sp..

Preparation of Kimchi and Salting (김치담금과 소금절임)

  • 김순동
    • Food Science and Preservation
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    • v.4 no.2
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    • pp.215-225
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    • 1997
  • The review was conducted to organize the desirable salting process from the literatures. In this study the principle of salting and effects of physicochemical changes in salting and salting factors such as cultivars of baechu(Chinese cabbage), concentration of salt, salting temperature, pH condition for salting and quality of kimchi were studied. The method of salting standard, treatment techniques in salting, and selection and mixture ratio of sub-ingredients were also reviewed. In future studies greater attention should be paid to salting and fermentation of kimchi.

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Effect of Ecklonia cava on the Quality Kimchi during Fermentation (감태 첨가가 배추김치의 숙성 중 품질에 미치는 영향)

  • Lee, Hyun-Ah;Song, Yeong-Ok;Jang, Mi-Soon;Han, Ji-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.1
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    • pp.83-88
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    • 2013
  • To improve kimchi qualities, Ecklonia (E.) cava of 5%, 15% or 25% were added and the qualities investigated by measuring changes in physicochemical, microbiological, and sensory characteristics during fermentation at $5^{\circ}C$. The pH of kimchi with E. cava added decreased and acidity increased. The number of Leuconostoc sp. in kimchi reached its maximum at the optimum fermenting stage, the $16^{th}$ day in control kimchi and the $20^{th}$ day in the kimchi added E. cava. The optimum fermenting stage of the kimchi was retarded due to adding the E. cava. The reducing sugar contents was highest in kimchi with 25% E. cava during the early stage of fermentation. According to Hunter's color values, the lightness of the kimchi with E. cava added decreased during fermentation, while the redness increased as fermentation proceeded. The sensory scores for overall acceptance, taste, texture and appearance were highest in kimchi with 15% E. cava.

Effect of the Beet Addition on the Quality of American Preferred Kimchi during Fermentation (비트 첨가가 미국인 선호 김치의 숙성 중 품질에 미치는 영향)

  • Yang, Yu-Jin;Han, Ji-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.4
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    • pp.538-543
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    • 2005
  • To improve the Quality of American preferred kimchi (APK), the APK added different ratios of beets were prepared and the Qualities of those kimchi were investigated by measuring the changes of physicochemical, microbiological and sensory characteristics during fermentation at $5^{\circ}C$. The pH of APK added beet (APKB) decreased as the amount of beet increased while total acidity gradually increased. The reducing sugar content was the highest in APKB added $3\%$ beet at early stage of fermentation. The number of Lactobacillus sp. and Leuconostoc sp. were the highest in $3\%$ beet added group during fermentation. In a texture experiment, the hardness of Korean standard kimchi used as control group showed rapidly decrease while the hardness of APKB were decreased slowly as fermentation proceeded. In Hunter's color values of APKB, lightness and redness increased as the amount of beet increased while yellowness decreased. Sensory scores of overall acceptance, taste, texture and appearance evaluated by Americans as sensory panels were the highest in APKB added $1\%$ beet. Americans also preferred fresh kimchi to optimum ripened kimchi. Therefore it was suggested that the American preferred kimchi added $1\%$ beet was the best group among American preferred kimchi groups.

The Change of Residual Chlorpyrifos during Fermentation of Kimchi (배추김치 숙성중 Chlorpyrifos 잔류량 변화)

  • Yun, Shuk-Ja
    • Korean Journal of Food Science and Technology
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    • v.21 no.4
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    • pp.590-594
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    • 1989
  • To determine the change of residual chlorpyrifos during Kimchi fermentation, the Kimchi was prepared and fermented at $4^{\circ}C$ for 4 weeks according to the conventional method. As a model experiment, chinese cabbages which were soaked in the chlorpyrifos solution were used for Kimchi preparation. It was found that the concentration of residual chlorpyrifos which was 0.161 ppm in raw cabbages decreased to 0.0938 ppm by 4 times of washing and further decreased to 0.0099ppm during fermentation of Kimchi for 4 weeks. In the model system, the residual chlorpyrifos decreased by the first order reaction as the fermentation of Kimchi proceeded . It's half life is approximately 1.8 weeks.

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Effect of Addition Methods of Green Tea on Fermentation Characteristics of Kimchi (녹차의 첨가방법이 김치의 발효특성에 미치는 영향)

  • 김경희
    • Food Science and Preservation
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    • v.9 no.4
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    • pp.406-410
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    • 2002
  • This study was conducted to investigate the effect of addition methods of green tea on the fermentation characteristics of kimchi. Kimchis were prepared to 3 methods such as Chinese cabbage soaked in brine containing 1% of green tea extracts(Ⅰ), salted the cabbage soaked in water containing 1% of green tea extracts(II), salted the cabbage mixed with seasonings containing 1% of green tea extracts(Ⅲ), and fermented at l0$\^{C}$. pH of Ⅰ∼Ⅲ kimchis were higher than that of control kimchi during fermentation and its acidity showed the same tendency. Total microbe(5.40∼8.38 log cfu/mL) and the number of lactic acid bacteria(4.99∼8.07 log cfu/mL) in Ⅰ∼Ⅲ kimchis were lower than those of control kimchi(6.16∼9.29 and 4.59∼8.42 log cfu/mL). Lightness of kimchi tissue showed the order of Ⅰ<II<control<Ⅲ. Sour taste and fishy taste of Ⅰ∼Ⅲ kimchis were lower than that of control kimchi. Overall quality was best in II estimated by sensory evaluation and fermentation characteristics.

Effect of Preparation Methods on Yulmoo Kimchi Fermentation (열무 물김치의 담금방법이 발효숙성에 미치는 영향)

  • 장명숙;피재은
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.6
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    • pp.990-997
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    • 1995
  • Effect of preparation method on Yulmoo kimchi(watery kimchi prepared with Yulmoo, leaf radish) fermentation was investigated by measuring physicochemical, microbiological and sensory properties for 20 days. Yulmoo kimchi was fermented at 4$^{\circ}C$ after keeping at room temperature(27$\pm$0.5$^{\circ}C$) for 8 hours. Four types(A, B, C, D) of Yulmoo kimchi were prepared. Sample A was made without wheat flour paste and red pepper powder and sample B was made with wheat flour paste. Sample C was made with red pepper powder, and sample D was made with wheat flour paste and red pepper powder. pH was slowly lowered and stabled after 10 days in all samples. Total acid content increased in all samples. Reducing sugar content initially increased and decreased thereafter. Especially, reducing sugar content of sample B increased in the early stage of fermentation and maintained initial reducing sugar content after 20 days. Total vitamin C content reduced during fermentation in all samples. Especially initial total vitamin C content of sample D increased more than those of other samples relatiely. Lightness and yellowness showed no difference, but on the other hand redness increased gradually in all samples. The number of lactic acid bactgeria reached maximum value in 10~13 days with the total cell numbers and gradually decreased thereafter in all samples. The number of lactic acid bacteria and total cell number of sample D was much more than those of any other samples. As a result of the sensory evaluation, Yulmoo kimchi showed significant difference in all characteristics. Sample D showed the highest scores in all characteristics before 10 days of fermentation. However, after 10 days sample B showed the highest scores.

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