• 식물조직배양학회지
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• 제25권3호
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• pp.141-146
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• 1998

[$^3$H] 5-epi-aristolochene (5-EAS)를 담배 현탁배양세포에 투여하여 elicitor에 의해 유도된 세포가 생합성하여 배지로 방출하는 [$^3$H]-capsidiol의 량을 측정함으로써 5-EAS hydroxylase의 활성을 검정하였고, 이 반응의 전 단계에 관여하는 효소인 sesquiterpene cyclase의 발현특성과 비교하였다. 5-EAS hydroxylase는 정상세포에는 전혀 활성을 보이지 않으나 elicitor로써 cellulase를 처리한 세포는 9시간 후부터 유도를 시작하여 18시간 후에 최대 활성을 보였는데 동일한 세포내에서 유도되는 cyclase와 유사한 패턴을 보였다. Cyt P450계 효소의 특이적 억제제로 알려진 ancymidol과 ketoconazole에 의해 5-EAS hydroxylase의 활성은 강한 억제를 보인 반면 cyclase의 활성은 억제를 보이지 않아 5-EAS hydroxylase가 P450계 효소임이 시사되었다.

• Journal of Microbiology and Biotechnology
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• 제33권2호
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• pp.180-187
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• 2023

The skin is a dynamic ecosystem on which diverse microbes reside. The interkingdom interaction between microbial species in the skin microbiota is thought to influence the health and disease of the skin although the roles of the intra- and interkingdom interactions remain to be elucidated. In this context, the interactions between Malassezia and Staphylococcus, the most dominant microorganisms in the skin microbiota, have gained attention. This study investigated how the interaction between Malassezia and Staphylococcus affected the antifungal susceptibility of the fungus to the azole antifungal drug ketoconazole. The susceptibility was significantly decreased when Malassezia was co-cultured with Staphylococcus. We found that acidification of the environment by organic acids produced by Staphylococcus influenced the decrease of the ketoconazole susceptibility of M. restricta in the co-culturing condition. Furthermore, our data demonstrated that the significant increased ergosterol content and cell membrane and wall thickness of the M. restricta cells grown in the acidic environment may be the main cause of the altered azole susceptibility of the fungus. Overall, our study suggests that the interaction between Malassezia and Staphylococcus influences the antifungal susceptibility of the fungus and that pH has a critical role in the polymicrobial interaction in the skin environment.

• 한국환경성돌연변이발암원학회지
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• 제26권2호
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• pp.53-58
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• 2006

Normally, environmental toxicants are classified as endocrine disruptors if they interfere with regulation of cellular function by endogeneous steroids through inhibition of receptor binding and/or transcriptional activation. So, many studies have been performed about agonist/antagonist of hormone receptor to study mechanisms of endocrine disruptors. If toxicants affect steroid biosynthesis and/or degradation and alter hormone homeostasis, these also are classified as endocrine disruptors. But there are not many studies of the mechanisms of endocrine disruptors on the basis of alteration of steroid biosynthesis and/or degradation. Isolation and culture of Leydig cells from testis is one of methods for the steroidogenesis screening assays to evaluate a substance for altering steroidogenesis. Leydig cells were harvested using the method described by Klinefelter with modifications. Leydig cells were purified by perfusion of testis and incubation ($34^{\circ}C$, 80cycles/minute, 20 minutes) with collagenase (0.25 mg/kg), centrifugal elutriation, percoll gradient centrifugation and BSA multidensity gradient centrifugation. To confirm if this method is one of appropriate tools to evaluate a substance for altering steroidogenesis, ketoconazole, positive control was administered to purified Leydig cells. Ketoconazole ($10^{-8}M$ and above) significantly reduced testosterone production in purified Leydig cells. From above results, we suggest that this method for steroidogenesis screening assay appears to be a appropriate tool to detect suspected compounds for altering steroidogenesis.

• 한국임상수의학회지
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• 제14권1호
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• pp.108-111
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• 1997

지방친화성 진균인 M. pachydermatis가 두 마리의 수캐에서 피부병의 원인균으로 증명되었다. 두 마리의 환축에서 흥반, 인설, 탈모 등이 여러 부위에서 발생하였으며, 광역항생물질과 코르티코스테로이드의 45일간 투여에 대해 아무런 임상적 반응을 나타내지 않았다. 발아를 나타내는 난원형 내지 원주형의 효모균이 KOH 및 PHOL 방법에 의해 환축의 피부병변으로부터 다수 증명되었고, 이들은 형태학적으로 M. pachydermatis와 일치하였다. 두 환축의 피부 병변부위 시료를 chloramphenicol을 첨가한 %37{\diamond}C$의 Saubouraud dextrose agar에 배양하면 이 진균이 반복하여 배양되었다. ketoconazole의 경구투여와 sulphur lime의 국소적용에는 치료반응을 잘 나타내었다.

• 한국환경생물학회:학술대회논문집
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• 한국환경생물학회 2005년도 추계학술대회 초록집
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• pp.87-87
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• 2005

• 한국임상수의학회지
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• 제15권1호
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• pp.184-187
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• 1998

In a harbor seal in captivity from the Yellow sea of Korea, alopecia began on flippers and tail, and spread to ventral part of abdomen and dorsal part of body in order. Multiples coalesced, and well-circumscribed lesions such as patches and maculas appeared on foreflipper, hind flipper, perianus, ventral part of tail, lips, and some parts of trunk were appeared in. Phialophora richardsiae, Gemella morbillorum, and coagulate-negative etaphyococci were isolated from Besions and hairs. After treatment with internal and external ketoconazole, povidone iodine, cephalexinl Pink Skin~l and multivitamins hair grew up and lesions were disappeared completely 8 months after occurrence.

• 대한한방내과학회지
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• 제29권4호
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• pp.846-855
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• 2008

Objects : The aim of this study was to investigate the influence of five herbal medicines on cytochrome P450 3A4 drug-metabolizing enzymes in human liver microsomes. Methods : To use human liver microsomes, an extract of five herbal medicines, which are Artemisia princeps Pampan, Sophora jeponica Linne, Panax notoginseng F. H. Chen, Lithospermum Erythrorhizon Sieb., and Cirsium maackii Maxim, which together are called Jihyulyak(止血藥, drugs for arresting bleeding, hemostatics), was co-incubated and measured for relative enzyme activity in incubation condition compared to ketoconazole, a representative inhibitor of CYP 3A4. Results : We showed that all five of the traditional herbal medicines had no inhibition effect of CYP 3A4 at 10, 20, 30, 40, and $50{\mu}g/ml$ doses in human liver microsomes, although Sophora japonica Linne(SJL) showed a little inhibition at about 81% inhibition rate of control. However, this result is not enough to prove that SJL has a CYP 3A4 inhibition effect. Moreover, we can't make sure that those rates had significant induction effect on CYP 3A4. Conclusions : The result of this study could support that those herbal medicines are safer than chemical drugs, even if this is the basic step to prove that result.

• 대한수의학회지
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• 제40권3호
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• pp.627-633
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• 2000

9.3kg의 6년령인 수컷 바셋하운드가 1년 이상 진행된 양측성 전두부위로부터 심한 점액화농성 분비물의 병력을 나타내었다. 내원당시 환축의 신체상태와 식욕은 불량하였다. 전두골위 피부에 형성된 누공으로부터 농성 삼출물이 양측성으로 분비되었다. 삼출물 시료를 배양하여 진균을 분리하였다. 방사선 검사에서는 전두동의 경계가 불분명하고 두꺼운 점액성막으로 덮여 있었으며 전두동의 혼탁도가 미만성으로 증가되어 있었다. 최종적으로 만성 진균성 전두동염으로 진단하였다. 전두동의 외과적 제거후 국소와 전신성 항진균요법으로 Chlorhexidine과 Ketoconazole을 적용하였다. 환축은 점진적으로 건강과 식욕을 회복하였다.

• 대한한의학회지
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• 제29권4호
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• pp.104-113
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• 2008

Objectives: The aim of this study was to investigate the influence of five herbal medicines on cytochrome P450 (CYP) 3A4 drug-metabolizing enzymes in human liver microsomes. Methods: By using of human liver microsomes, we extracted Cnidium officinale Makino, Rhus verniciflua Stokes, Prunus persica Batsch, Corydalis remota Fisch, Carthamus tinctorius Linne, which are called Hwalhyulgeoouhyak(活血祛瘀藥). Then they were incubated and measured for relative enzyme activity under incubation conditions compared to ketoconazole, which is known as a representative inhibitor of CYP 3A4. Results: We showed that all of five traditional herbal medicines had no inhibition effect of CYP 3A4 at 10, 20, 30, 40, and 50${\mu}g/m{\ell}$ doses in human liver microsomes, although Rhus verniciflua Stokes (RVS) showed a little inhibition as about 95% enzyme activity of control. However, this result was not enough to prove that RVS has a CYP 3A4 inhibition effect. Moreover, we can't confirm that those rates have significant induction effect on CYP 3A4. Conclusions: The result of this study could support that those herbal medicines are more reliable than chemical drugs, even if this is a basic step to prove that result.

• 한국임상수의학회지
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• 제20권2호
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• pp.242-244
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• 2003

3연령 잡종편이 소양감, 탈모의 증상으로 내원하였다. 신체검사에서 전신적으로 발적, 구진, 탈모, 비듬 그리고 가피의 병변이 관찰되었다. 전반적인 피부검사결과 개선충, Staphylococcus spp과 Malassezia spp의 혼합 감염으로 진단되었다. Selamectin 6 mg/kg을 2주간격으로 2회 경부피부에 적용하였다. 그리고 한 달간 구균과 Malassezia 치료를 위해 amoxicillin trihydrate/clavulanate pattassium과 ketoconazole 10 mg/kg을 경구투여하고 5% chlorhexidine 샴푸를 주2회 실시하였다. 첫 치료 후 7,14,21 그리고 28일에 피부를 재검사 하였을 때 개선충은 관찰되지 않았으며 피부 증상은 현저하게 개선되었다. 피모는 거의 정상으로 회복되었으며 Staphylococcus와 Malassezia spp는 거의 관찰되지 않았다. 그러므로 이 치료절차에 따른 selamectin 적용은 개선충이 감염된 개에서 매우 효과적이라고 사료된다.