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Antimicrobial Activities of Organic Extracts from Fruit of Thuja orientalis L. (측백나무 열매 추출물의 항균활성)

  • Youm, Tae-Hyun;Lim, Heung-Bin
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.5
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    • pp.315-322
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    • 2010
  • This study was carried out to investigate the antimicrobial activities of organic extracts obtained from the fruit of Thuja orientalis L. The native fruits in Korea were collected and extracted by 80% ethanol, and the extract was sequentially fractionated with n-hexane, chloroform, ethylacetate, and butanol. The fraction yields of n-hexane, chloroform, ethylacetate, butanol and water of ethanol extract were 10.15%, 10.05%, 1.45%, 45.35% and 27.55%, respectively. n-Hexane-soluble fraction showed the highest antibacterial activity against gram positive bacteria, while the chloroform, ethylacetate, butanol and aqueous fractions did not show any antibacterial activity. Minimum inhibitory concentration (MICs) on Staphyloycoccus aureus, Bacillus subtilis and Sateptococcus pneumoniae, n-hexane-soluble fraction were $100\;{\mu}g$, $500\;{\mu}g$ and $50\;{\mu}g$/disc, respectively. The antibacterial activity was not destroyed by heating at 80, 100, $120^{\circ}C$ for 30 min and was not affected by pH. In the inhibitory test against the Staphylococcus aureus, Bacillus subtilis and Sateptococcus pneumoniae, n-hexane-soluble fraction showed potent growth inhibition at the concentration of 0.1 and $0.5\;{\mu}g/mL$ for 12~24 hours and n-hexane-soluble fraction did not show any mutagenic activity.

Relationships of Midrib Ratio to Some Agronomic Characters in Burley Tobacco(N.tabacum L.) (버어리종 잎담배의 주맥비율과 형질 상호간의 관련성)

  • Jo Chun-Joon;Choi Sang-Ju;Jung Suk-Hun
    • Journal of the Korean Society of Tobacco Science
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    • v.26 no.2 s.52
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    • pp.73-78
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    • 2004
  • This study was conducted to find out the relationship among agronomic characteristics including midrib ratio in Burley tobacco(N.tabacum L.) Four cultrivars, Burley 21, KB108, KB111 and KB301, were grown at Suwon Experimental Station in 2001. Several agronomic characteristics of these cultivars were measured for the analysis of correlations among agronomic characteristics. Midrib ratio of KB301 was somewhat higher than those of the other cultivars, but there is no significant differences among cultivars at $5\%$ probability level. Among four stalk positions, midrib ratio of cutters was the highest. Midrib ratio was correlated positively with length of leaf at $1\%$ level and negatively with weight of lamina at $5\%$ level, respectively.

Simplified approach for symbol error rate analysis of SC-FDMA scheme over Rayleigh fading channel

  • Trivedi, Vinay Kumar;Sinha, Madhusudan Kumar;Kumar, Preetam
    • ETRI Journal
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    • v.40 no.4
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    • pp.537-545
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    • 2018
  • In this paper, we present a comprehensive analytical study of the symbol error rate (SER) of single-carrier frequency-division multiple access (SC-FDMA) with zero-forcing frequency domain equalization (ZF-FDE) over a Rayleigh fading channel. SC-FDMA is considered as a potential waveform candidate for fifth-generation (5G) radio access networks (RANs). First, the $N_C$ fold convolution of the noise distribution of an orthogonal frequency-division multiplexing (OFDM) system is computed for each value of the signal-to-noise ratio (SNR) in order to determine the noise distribution of the SC-FDMA system. $N_C$ is the number of subcarriers assigned to a user or the size of the discrete Fourier transform (DFT) precoding. Here, we present a simple alternative method of calculating the SER by simplifying the $N_C$ fold convolution using time and amplitude scaling properties. The effects of the $N_C$ fold convolution and SNR over the computation of the SER of the SC-FDMA system has been separated out. As a result, the proposed approach only requires the computation of the $N_C$ fold convolution once, and it is used for different values of SNR to calculate the SER of SC-FDMA systems.

Antimutagenic Effects of Linoleic Acid

  • Lim, Sun-Young;Rhee, Sook-Hee;Park, Kun-Young
    • Preventive Nutrition and Food Science
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    • v.2 no.1
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    • pp.29-34
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    • 1997
  • In order to determine the effectiveness of linoleic acid(LA) to inhibit carcinogens/mutagens-induced mu-tagenesis, Ames test using Salmonella typhimurium TA100 and the SOS chromotest using E. Coli PQ37, were carried out. The inhibitory effect of LA(1%) on the Ames mutagenicity test were 98%, 78%and 69% mediated by aflatoxin B₁(AFB₁), N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) and 4-nitroquinoline-1-oxide(4-NQO), respectively. LA exhibited a strong antimutagenic activity aganist indirect mutagen, AFB₁whereas exhibited the same concentration of LA showed weaker inhibitory effects on direct mutagens of MNNG and 4-NQO than that AFB₁. LA also reduced the SOS responses induced by MNNG and 4-NQO significantly. This result showed a possibility that LA can be a protective agent in early step of cancinogenesis.

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Effect of the Gardenia jsaminodes and Glycine max on Hepatotoxicity of D-Galactosamine in Rats (D-Galactosamine으로 유발된 흰쥐의 간손상에 대한 치자와 두시 추출액이 미치는 영향)

  • Kim, Jeong-Sang;Jeong, Jong-Kil
    • 대한상한금궤의학회지
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    • v.4 no.1
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    • pp.29-36
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    • 2012
  • Aim : To investigate the hepatotective effect of Gardenia jsaminodes and Glycine max aqueous extract against D-galactosamine (d-GalN, 300mg/kg body weight) was administered to the male Sprague Dawley (SD) rats. Materials and Methods : The study was carried out on male SD rats (age matched, weight $250{\pm}10g$). Experimental groups divided four: Normal group (Nor) was administered saline, Control (Con) group was administered saline after d-GalN treatment. Experimental group (Exp) was administered Gardenia jsaminodes (200 mg/kg; Ga group), Glycine max (700 mg/kg; Gl group), and Gardenia jsaminodes+Glycine max (200 mg/kg+700 mg/kg, GG group) during 14 days(n=5). Results : d-GalN administration induced hepatotoxicity in rats which was manifested by increased levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase but decreased total cholesterol and triglyceride. Treatment with Gardenia jsaminodes extract significantly protected the liver in d-GalN administered rats. Conclusion : Gardenia jsaminodes aqueous extract and Gardenia jsaminodes+Glycine max extract possesses hepatoprotective potential, thus validating its use in alleviating toxic effects of d-GalN.

Nanoscale Longitudinal Normal Strain Behavior of ${Si_3}{N_4}$-to-ANSI 304L Brazed Joints under Pure Bending Condition

  • Seo, D.W.;Lim, J.K.
    • International Journal of Korean Welding Society
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    • v.4 no.1
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    • pp.46-52
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    • 2004
  • To combine the mechanical advantages of ceramics with those of metals, one often uses both materials within one composite component. But, as known, they have different material properties and fracture behaviors. In this study, a four-point bending test is carried out on $Si_3N_4$ joined to ANSI 304L stainless steel with a Ti-Ag-Cu filler and a Cu interlayer at room temperature to evaluate their longitudinal strain behaviors. And, to detect localized strain, a couple of strain gages are pasted near the joint interfaces of the ceramic and metal sides. The normal strain rates are varied from $3.33{\times}10^5$ to $3.33{\times}10^{-1}s^{-1}$ Within this range, the experimental results showed that the four-point bending strength and the deflection of the interlayer increased with increasing the strain rate.

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Effect of Forms and Levels of Nitrogen Fertilizer on Plant Growth and Essential Oil Content of Agastache rugosa

  • Ohk, Hyun-Choong;Song, Ji-Sook;Chae, Young-Am
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.2
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    • pp.128-133
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    • 2000
  • This study was carried out to investigate the effect of forms and levels of nitrogen fertilizer on plant growth and essential oil production of Agastache rugosa. Calcium nitrate had more influenced on length and width of leaves and lateral branch length than did urea. When nitrogen fertilizer level was increased from 12 kgN/I0a to 24kgN/I0a, plant growth was stimulated and dry matter of leaf and inflorescence were increased. Top dry matter of plant with calcium nitrate treatment (38.4 g) was heavier than that of urea treatment (32.8 g). Interactions among accession and nitrogen form and nitrogen rate were not significantly different for top dry matter. The forms and rate of nitrogen fertilizer did not affect estragole content. The estragole contents was higher in leaf (91.8%) than that of inflorescence (81.3%). While the essential oil content was not affected by different nitrogen forms, nitrogen level affected the essential oil contents positively by increasing dry matter. Essential oil yield was not affected by accession or nitrogen form, but by nitrogen rate. With increasing N application from 12kgN/I0a to 24 kgN/I0a, essential oil yield was increased by 95.8 %.

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Suggestion and Design of GaN on Diamond Structure for an Ideal Heat Dissipation Effect and Evaluation of Heat Transfer Simulation as Different Adhesion Layer (이상적인 열방산 효과를 위한 GaN on Diamond 구조의 제안과 접합매개층 종류에 따른 열전달 시뮬레이션 비교)

  • Kim, Jong Cheol;Kim, Chan Il;Yang, Seung Han
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.30 no.5
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    • pp.270-275
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    • 2017
  • Current progress in the development of semiconductor technology in applications involving high electron mobility transistors (HEMT) and power devices is hindered by the lack of adequate ways todissipate heat generated during device operation. Concurrently, electronic devices that use gallium nitride (GaN) substrates do not perform well, because of the poor heat dissipation of the substrate. Suggested alternatives for overcoming these limitations include integration of high thermal conductivity material like diamond near the active device areas. This study will address a critical development in the art of GaN on diamond (GOD) structure by designing for ideal heat dissipation, in order to create apathway with the least thermal resistance and to improve the overall ease of integrating diamond heat spreaders into future electronic devices. This research has been carried out by means of heat transfer simulation, which has been successfully demonstrated by a finite-element method.

Study of plasma β-carotene concentration in dairy cows (젖소의 혈장 베타카로틴 농도 조사)

  • Nam, Hyang-mi;Moon, Jin-san;Joo, Yi-seok;Oh, Tae-ho;Park, Yong-ho;Han, Hong-ryul
    • Korean Journal of Veterinary Research
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    • v.39 no.5
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    • pp.1021-1027
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    • 1999
  • This study was carried out to determine the effects of ${\beta}-carotene$ on the control of mastitis in dairy cows during the dry period. The relationship between the levels of plasma ${\beta}-carotene$ and the status of udder health in Holstein dairy cows were investigated. Blood samples were collected from 117 cows to compare the levels of plasma ${\beta}-carotene$ in lactating cows. The levels of plasma ${\beta}-carotene$ were $1.82{\mu}g/ml$ in healthy cows(n = 65) and $1.12{\mu}g/ml$ in mastitic cows(n = 52), respectively(p < 0.01). In the experiment to compare the level of plasma ${\beta}-carotene$ in the cows at different stages of lactation, the plasma ${\beta}-carotene$ levels were $1.73{\mu}g/ml$ in lactating cows(n = 22), $1.29{\mu}g/ml$ in nonlactating cows(n = 35) and $0.43{\mu}g/ml$ in cows after calving(n = 16)(p < 0.05).

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In Vitro Glycosylation of Peptide (RKDVY) and RNase A by PNGase F

  • Park, Su-Jin;Lee, Ji-Youn;Park, Tai-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.13 no.2
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    • pp.191-195
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    • 2003
  • The in vitro glycosylation of pentapeptide (Arg-Lys-Asp-Val-Tyr; RKDVY) and RNase A was carried out using PNGase F (peptide-N-glycosidase F), and the results were analyzed using MALDI-TOF-MS. Aminated N,N-diretyl chitobiose was used as the sugar in the glycosylation reaction, and the amination yield of N,N'-diacetyl chitobiose was about $60\%$. To reduce the water activity and shift the reaction equilibrium to a reverse reaction, 1,4-dioxane or ethylene glycol was used as the organic solvent in the enzymatic glycosylation. A certain extent of nonenzymatic glycosylaton, known as the Maillard reaction, was also observed, which occurs on an arginine or lysine residue when the length of tie sugar residue is one or two. However, the extent of glycosylation was much higher in the enzymatic reaction, indicating that PNGase F can be effectively used to produce glycopeptides and glycoproteins in vitro.