• Korean Journal of Veterinary Research
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• v.32 no.3
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• pp.295-308
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• 1992

In order to study on the Sertoli cell, we attempt have been made to measure the average number of each germ cells per Sertoli cell on the 12 stages of cycle in matured korean Jindo dog. The fine structure of Sertoli cell junctional specialization was studied with electron microscope. The results were summarized as follows; 1. The average number of various germ cells associated with Sertoli cell was 9.77 to 13. 80 through stages of cycle and the total average number was 11.62. 2. Sertoli-Sertoli cell junctional specialization was present in seminiferous epilthelium, and Sertoli-spermatid cell junctional specialization rose from stage 8 spermatid, persisted to step 13 spermatid and then disappeared. The structure of Sedoli-spermatid cell juncticnal specialization was not similar to that of Sertoli cxlls. 3. Just after spermiation, free-surface of Sertoli-spermatid cell junctional specialization was replaced by Sertoli cell cytoplasm with tubulobulbar complex at the neiglaboring region observed. 4. The Sertoli cell process was located within the cytoplasm of late stage spermatids. Some membranes of residual body and spermatid cytoplasm partly disappeared, resulting in opening of the cytoplasm of spermatid into that Sertoli cell. This fact suggested that spermatid cytoplasm was partly eliminated.

• Korean Journal of Veterinary Research
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• v.32 no.3
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• pp.281-293
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• 1992

Classification of the cycle of seminiferous epithelia into 12 stages by the morphological changes in acrosomal system and evaluation of the relative frequency of stages and the cell association were histologically performed in the mature Korean native Jin-do dogs. The results were summarized as follows; 1. The minimum number of type A spermatogonia averaged 1.01 at stages I, while maximum number averaged 2.47 at stages XII. Some type A spermatogonia divided at stage XII to produce the type intermediate(IN) spermatogonia at the following stage I. The type IN spermatogonia divided at stage IV to produce the type B spermatogonia at stage V. 2. The type B spermatogonia divided at stage VI to produce the preleptotene primary spermatocytes at stage VII. The secondary spermatocytes observed at stage XII. The secondary spermatocytes observed at stage XII divided to give rise to the round spermatids at the following stage I. The numbers of the first spermatocytes and spermatids were almost constant, respectively, through all the cycles of seminiferous epithelium. 3. The acrosomal vesicle was invaginated to occupy one third to half of spermatid nucleus at the cap phase, which was different from that of rodent and ruminant spermatid nuclei. 4. The relative frequencies of stages I to XII of seminiferous epithelia cycle were 10.34, 4.84, 5.03, 8.22, 10.86, 6.63, 6.42, 18.88, 10.17, 6.18, 7.62% and 4.81%, respectively.