The purpose of this study was to investigate the morphology and intercellular junctions of the odontoblast of dental pulp in the rat incisor by means of the freeze fracture electron microscopy. Twenty male Sprague-Dawley rats weighing $150{\sim}200g$ were used. After being anesthetized by an intraperitoneal injection of 0.5 ml sodium pentobarbital per kg in body weight(60 mg/ml) the animals were perfused with 2.5% glutaraldehyde-2% paraformaldehyde fixative in 0.1 M cacodylate buffer, pH 7.2 through the ascending aorta for one hour. The incisors were carefully extracted from the jaws and demineralized by suspending them in 0.1 M EDTA in 3% glutaraldehyde (pH 7.2) for two weeks. After demineralization, the specimens were obtained from the portion divided into five equal parts. For freeze-fracture replication, demineralized tissues were infiltrated for several hours with 10%, 25% glycerol in 0.1M cacodylate buffer as a cryoprotectant and then frozen in liquid Freon 22 and stored in liquid nitrogen. Fracturing and replication were done in Balzers BAF 400D high-vacuum freeze-fracture apparatus at $-120^{\circ}C$ under routine $5X10^{-7}$ Torr vacuum. The tissue was immediately replicated with platinum unidirectionally at $45^{\circ}$ angle and reinforced with carbon at $90^{\circ}$ angle unidirectionally or by using a rotary stage. The replication process was monitored by a quartz-crystal device. The replicas were immersed in 100% methanol overnight. The tissue was then digested from the replica by clorox (laundry bleach), placed into 5% EDTA, and washed repeatedly with distilled water. The replicas were picked up on 0.3% formvar-coated 75 mesh grids and examined in the JEOL 100B electron microscope. The results were as follows; 1. Both in thin sections and freeze-fracture replicas, three types of intercellular junctions were recognizable in the plasma membrane of odontoblast: gap junction, tight junction and desmosome-like junction. 2. The nuclear pores were evenly distributed over the nuclear envelope. The pore complex formed a ring about 70 nm in diameter. 3. Gap junctions were found between odontoblasts as well as odontoblasts and neighbouring pulp cells (fibroblast, subodontoblastic cell process, nerve-like fibre). Gap junctions, which were round, ellipsoid and pear-shaped and 600 nm in diameter, were observed in the odontoblast. 4. Numerous round and ellipsoid gap junctions could be frequently seen on the plasma membranes in cell body and apical part of the odontoblasts. On the P face, the junctions were recognized as a cluster of closely packed particles, measuring about 9 nm in diameter, and on the E face, the junctions were recognized as a shallow grooves.
Journal of the Korea Academia-Industrial cooperation Society
/
v.15
no.4
/
pp.2150-2159
/
2014
This study was designed to investigate the status of orthodontic treatment and facial plastic surgery among 389 patients who visited dental clinics located in Seoul and Gyeonggi Province. After the collection of data, an analysis was carried out using a spss statistical program. The analysis results are as follows. the level of satisfaction with orthodontic treatment from patients with experience of the orthodontic treatment turned out to be high(p<0.01). Women considered 3.01 to 4 million won to be reasonable cost for orthodontic treatment (p<0.05), which is higher cost recognized by men, and patients with experience of orthodontic treatment recognized 19 to 24 months as the proper orthodontic treatment period(p<0.01). In addition, women showed higher figures than men in their awareness on the need for orthodontic treatment checkups(p<0.001), and facial plastic surgery experience (p<0.01). With respect to the part of the face on which they hoped to have plastic surgery, eye plastic surgery was higher in women (p<0.01), and nose surgery turned out to be higher in patients with experience of orthodontic treatment(p<0.05). Meanwhile, women considered the eye as the most important part among other facial areas (p<0.001), and they recognized that dentist should perform plastic surgery on both jaws among facial areas(p<0.01). In conclusion, the dental medical staff should provide consultations and operate treatment systems in consideration of gender differences and needs of dental patients. In the case of orthodontic treatment for the improvement of facial appearance, especially double jaw surgery which is closely related to the oral function, professional dental medical staff needs to perform the operation, considering the special oral environment. In addition, it is determined that provision of relevant medical information and promotion of dental service is required to provide patients with correct knowledge about orthodontic treatment and facial plastic surgery.
Class III malocclusion patients can be approached with many different types of treatment methods, and thus, each patient's problems must be accurately evaluated to allow selection of the best possible treatment method. Cephalometric analysis is an essential part of diagnosis and treatment planning of orthodontic patients, and it would certainly be helpful if reliable cephalometric guidelines could be set. The author divided 482 Class III malocclusion patients(253 males and 229 females) into fourgroups according to different types of treatment methods they have received to correct imbalance between upper and lower jaws: 1) orthopedic appliance (face mask & RPE), 2) camouflage treatment with fixed appliance, 3) surgical-orthodontic treatment, 4) cross-bite correction with removable plates/ functional appliance. Cephalometric values at the time of first clinical examination were compare among the four groups. Cephalometric analysis indicates the following results: 1)the amounts of antero-posterior and vertical skeletal discrepancies and dental compensation were greatest in surgery group 2) SNB, Wits, distance from Nasion Perpendicular Plane to point a facial angle, facial convexity, and APDI were greater in orthopedic appliance group than fixed appliance(camouflage) group, but there was no statistical difference 3) removable plates/ functional appliance group showed least amounts of skeletal discrepancies and dental compensation with statistical significance.
Journal of the korean academy of Pediatric Dentistry
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v.43
no.4
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pp.452-460
/
2016
Burkitt lymphoma (BL) is an aggressive form of non-Hodgkin's B-cell lymphoma found primarily in the pediatric population. In the oral cavity, this tumor can grow rapidly and often brings about facial swelling or development of an exophytic mass involving the jaws. A 5-year-old boy was referred for swelling and pain in the left mandibular area. The patient showed diffuse swelling on the left side of the mandible and firm-moderate tenderness upon palpation. An intraoral examination showed moderate mobility and sensitivity to percussion on the left primary first and second molars, without severe caries. A radiographic examination revealed complete loss of the lamina dura on the left primary second molar and permanent first molar. There was a radiolucent osteolytic lesion and destruction of the cortical bone of the left mandibular body. Based on the clinical, radiographic, and immunohistochemical findings, the patient was diagnosed with BL, and was referred to a pediatrician for systemic evaluation and intensive chemotherapy. Even before the completion of chemotherapy, the swelling resolved and the displaced teeth were relocated to a normal position. This patient showed a good prognosis due to prompt diagnosis and intensive chemotherapy. Early diagnosis and referral for treatment can prevent the development of BL.
Cleft lip and palate is the most frequent congenital facial deformity of the orofacial area. Successful management of patients with cleft lip / palate requires a multidiciplinary approach from birth to adult stage. Coordinated treatment by the cleft palate team is an essential requirement to obtain optimum treatment results. One of the negative effect of the early surgical interventions of lip and palate is a significant incidence of maxillary growth restriction that produces secondary deformities of the jaws and malocclusion that includes congenital missing of lateral incisor, malformed teeth, rotation or ectopic position of upper anterior teeth, and it has been thought due to the resistance of palatal scar tissue. In Orthodontic treatment for cleft lip / palate patients, expansion of upper dental arch or palatal suture is often needed to correct posterior and/or anterior cross bite and align upper teeth. Various appliances such as hyrax, quad-helix, fan-type expansion screw and jointed-fan type expander can be used for palatal expansion. In the orthodontic treatment of the cleft lip / palate patient, we must consider patient age and severity of palatal constriction for proper appliance selection, and must pay special attention to maintain the treatment results.
Park, Sung-Youn;Nam, Dong-Woo;Kim, Hyun-Jung;Kim, Young-Jin;Nam, Soon-Hyeun
Journal of the korean academy of Pediatric Dentistry
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v.30
no.3
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pp.489-494
/
2003
Odontomas are the common type of odontogenic tumors and generally they are asymptomatic, depending upon size, location and their limited growth potential. they are rarely diagnosed before the second decade of life, and the frequently lead to impaction or delayed eruption of permanent teeth. Odontomas are classified of compound as compound or complex by morphology. Complex odontomas are unorganized masses of odontogenic tissues, morphologically not resembling the teeth, account for approximately 25 percent of all odontomas, 22 percent of odontogenic tumor of the jaws, and have a predilection for the posterior mandible in males. The etiology of odontomas is unknown, although local trauma, infection, and genetic factors have been suggested. Usually, treatment of odontoma is conservative sugical removal and their is little probability of recurrence. This paper describes two cases of complex odontomas diagnosed in children due to impaction of maxillary first molar in all cases, the surgical excision of the lesions was performed. Follow-up after 2 years, showed spontaneous eruption of the first permanent molar to the occlusal plane.
This study was aimed at how posteroanterior cephalometric measurements affect the perception of the patient about his/her facial asymmetry. One hundred orthodontic patients, over the age of 12, who required a posteroanterior cep-halogram in the Department of Orthodontics of Chonnam National University Hospital were used as the subjects. They were asked if they thought their faces were asymmetrical. Their responses were classified into 5 groups based on the level of asymmetry as follows : Definitely No, Probably No, Don't Know, Probably Yes, and Definitely Yes. Nine linear and low angular measurements from each posteroanterior cephalometric radiographs were analysed on the standard of the line between crista galli and anterior nasal spine to show the extent of asymmetry. Through this comparative study, the following results were obtained. 1. As the deviation of menton and the midline discrepancy of the upper and lower jaws were greater, the perception of patients about their facial asymmetry was higher. 2. All the measurements from the group 'Don't Know' showed no statistical difference from those of the groups 'Definitely No' or 'Probably No.' 3. All the measurements from the group 'Probably Yes' showed no statistical difference from those of the group 'Definitely yes.' .
Objective: The purpose of this study was to investigate the horizontal skeletal pattern and dental arch differences between Class III malocclusion patients and normal occlusion patients. Methods: Twenty skeletal Class III malocclusion patients and ten normal occlusion patients were selected and 3D facial CT were taken to analyze the horizontal skeletal differences between the two groups. Results: In the horizontal comparison of the maxilla, skeletal width and perimeter were significantly smaller in skeletal Class III patients on ANS and A point reference planes. The difference between maxillary width of ANS and A point reference planes showed that there was greater constriction of the first and second premolar in skeletal Class III patients. In the horizontal comparison of the mandible, the widths of the canine and premolar area were significantly larger in skeletal Class III patients on B point reference plane. The differences between width of the upper and lower jaws (comparison of A and B reference planes) were significantly large in the canine and premolar area. Conclusions: From this study, the characteristics of Class III malocclusion patients were shown through horizontal constriction of the maxilla. But to make clear further detailed characteristics of Class III malocclusion patients, additional studies are necessary.
Journal of the korean academy of Pediatric Dentistry
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v.34
no.3
/
pp.532-542
/
2007
The purpose of study was to review the transition of dentition according to the evolution of man to know the background of the dental problems like hypodontia and malocclusion. Man is Kingdom Animalia, Phylum Chordata, Class Mammalia, Order Primates, Suborder Haplorrhini, Superfamily Hominoidea, Family Hominidae, Genus Homo, Species Sapiens by taxonomy. The first hominid was Australopithecus which appeared c. 4 millions of years ago and showed bipedalism and distinct dentition. Homos began with H. habilis who appeared c. 2.5 millions of years ago and made stone tools, and then H. erectus and H. neanderthalensis appeared and disappeared until H. sapiens came. The dental formula of primitive mammalians which was I3 C1 P4 M3 changed to I2 C1 P4 M3 of primitive primates, to I2 C1 P3 M3 of Haplorrhini, and to I2 C1 P2 M3 of hominoids. That of H. sapiens is changing to I2 C1 P2 M2.The box type dentition of hominoids changed to the omega type dentition of Australopithecus, and to the parabolic type of H. sapiens. The size of teeth decreased continually, especially the canine and sexual dimorphism. The dentition moved backward and downward to the cranial crown according to the increase of the brain and decrease of the jaws. It was suggested that the change of diet to the starchy foods, food processing, and the development of cooking reduced the necessity of mastication and caused the change of dentition. The future of H. sapiens who is quite a new species in the earth histroy and is now causing the mass extinction of other species is hard to see. It seems that hypodontia and malocclusion are related to the dentition change according to the evolution of man and is likely to increase.
Journal of Dental Rehabilitation and Applied Science
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v.17
no.4
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pp.245-256
/
2001
The purpose of this study is to analyze the mechanical stress and displacement on the jaws during the simulated bilateral clenching task on the three-dimensional finite element model of the dentated skull with unilateral molar loss. For this study, the computed tomography(G.E.8800 Quick, USA) was used to scan the total length of human skull in the frontal plane at 2.0mm intervals. The fully assembled finite element model consists of the articular disc, maxilla, mandible, teeth, periodontal ligament and cranium. The FE model was used to simulate the bilateral clenching in intercuspal position. The loading condition was the force of the masseter muscle exerted on the mandible as reported by Korioth et al. degrees of freedom of the zygomatic region where the masseter muscle is attached were fixed as restraints. In order to reflect the actual action of the muscles force, the displacement of the region was attached where the muscle is connected to the temporal bone and restraint conditions were given values identical to values at the attachment region of the masticatory muscle but with the opposite direction of the reaction from when the muscle force is acted on the mandible. Although the mandible generally has higher displacement and von Mises stress than the maxilla, its mandibular corpus on the molar-loss side has a higher stress and displacement than the molar-presence side. Because the displacement and von Mises Stress was the highest on the lateral surface of mandibular corpus with molar loss, the stress level of the condyle on the molar-loss side is greater than that of the molar-presence side, which in turn caused the symphysis of the mandible to bend. In conclusion, the unilateral posterior bite collapse with molar loss under para-functional activities such as bruxism and clenching can affect the stress concentration on the condyle and mandibular corpus. It is therefore necessary to consider the biomechanical function of dento-skeleton under masticatory force while designing the occlusal scheme of restoration on alveolar bone with the posterior collapse.
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