• Journal of Life Science
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• v.24 no.2
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• pp.137-147
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• 2014

Cortex ulmi pumilae, the cortex of Ulmus davidiana var. japonica, has been used in traditional folk medicine for its anti-inflammatory effect. Although its various bioactivities such as anti-inflammatory, anti-microbial, and anti-cancer, have been reported, the anti-adipogenic activity of cortex ulmi pumilae remains unclarified. In the present study, we investigated the effect of cortex ulmi pumilae extract on adipocyte differentiation in 3T3-L1 preadipocytes. Treatment with cortex ulmi pumilae extract significantly reduced the formation of lipid droplets and triglyceride content in a dose-dependent manner; this is associated with an inhibition of the adipogenic transcription factors, CCAAT/enhancer binding protein ${\alpha}$ ($C/EBP{\alpha}$), $C/EBP{\beta}$, and peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$). In addition, cortex ulmi pumilae extract treatment during the early stage of adipogenesis showed more efficient anti-adipogenic activity than treatment during other stages of adipogenesis. Cortex ulmi pumilae extract also inhibited cell proliferation and induced G1 arrest of 3T3-L1 cells in the early stage of adipogenesis. This was associated with upregulated expression of Cdk inhibitor p21 and downregulated expression of cyclin E and phospho-Rb, indicating that cortex ulmi pumilae extract blocks mitotic clonal expansion by cell cycle regulation. Taken together, these results suggest that cortex ulmi pumilae extract possesses anti-adipogenic activity through the inhibition of adipocyte differentiation by blocking mitotic clonal expansion.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.3
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• pp.167-173
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• 2002

This experiment was conducted to figure out the change of soil physical properties, rice growth and yield with the years of continuous cultivation in direct dry-seeding and no-tillage machine transplanting. Experiments were conducted at NHAES(National Honam Agricultural Experiment Station, RDA, Iksan, Cheon Buk Province, South Korea) with a rice variety "Dongjinbyeo" from 1995 to 2000. In no-tillage machine transplanting cultivation, organic matter in soil was higher than that on direct dry-seeding and was significantly high in topsoil. Problematic weed species were E. crus-galli B., A. keisak H., and L. japonica M. Plant height and tiller number m-2 were higher in common-tillage during the total growth duration. The highest weedy rice occurrence of 27.5% was observed in live years' continuous direct dry-seeding and followed by 6.2%, in four years', and 3.7%, in three years'. The highest yield reduction of 38% was observed in five years' continuous direct dry-seeding. The reduction may resulted from the competition between weedy rice and cultivated rice.

• Korean Journal of Food Science and Technology
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• v.37 no.2
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• pp.233-240
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• 2005

To discover new functional materials using edible plants, antioxidant activities of methanol extracts from various parts of seven wild vegetables were investigated in vitro. Total polyphenol contents, determined by Folin-Denis method, varied from 16.74 to $130.22{\mu}g/mg$. Radical-scavenging activities of methanol extracts were examined using ${\alpha},\;{\alpha}-diphenyl-{\beta}-pirrylhydrazyl$ (DPPH) radicals and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assay. Inhibition effects on peroxidation of linoleic acid determined by ferric thiocyanate (FTC) method and on oxidative degradation of 2-deoxy-D-ribose in Fenton-type reaction system were dose-dependent. Athyrium acutipinulum Kodama (leaf and rood), Achyranthes japonica (Miq.) Nakai (seed), and Solidago virga-aurea var. gigantea Nakai (root) showed relatively high antioxidant activities in various systems.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.370-377
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• 2014

Research was initiated to evaluate cool-season grass (CSG) overseeding effects on coverage and species transition in Korean lawngrass (Zoysia japonica Steud.) and to determine CSGs and their seeding rate for a practical overseeding. Treatments were comprised of Kentucky bluegrass (KB, Poa pratensis L.), perennial ryegrass (PR, Lolium perenne L.), tall fescue (TF, Festuca arundinacea Schreb.) and their mixtures. Significant differences were observed in turfgrass coverage and species transition. Since overseeding, the coverage was highest in PR, followed by TF and lowest in KB. The highest coverage in polystand was associated with Mixture IV (TF 50 + PR 50) at a seeding rate of $150gm^{-2}$ which had highest PR and TF, but without KB. Regarding turfgrass transition, zoysiagrass recovery continued to come back up to 80% from late March. But it reduced after early October. Zoysiagrass in monostand was greatest in KB and lowest in TF, but variable in polystand. For the fast and great coverage in winter, it would be the best to apply with PR at $100gm^{-2}$ and equal combination of PR and TF by 1/2 in the mixture at $150gm^{-2}$. For the smooth spring transition to zoysiagrass, however, KB application at $50gm^{-2}$ is recommended.

• Korean journal of applied entomology
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• v.48 no.3
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• pp.285-294
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• 2009

A study was conducted to find out plant flora, the occurrence of insect pests and natural enemies in the fallow paddy fields of Jeonnam and Jeonbuk provinces from 2004 to 2005. They were identified as 124 plant species in 32 families, 58 insect pest species in 30 families and 11 natural enemy species in 9 families. Plant species belonging to Poaceae and Asteraceae were founded as 28 and 18 species, respectively, and dominated significantly among plants growing in the fallow paddy fields. In insect pests, Conocephalus chinensis, Nysius plebejus, Cletus punctiger, Eysarcoris aeneus, Nephotettix cincticeps, Laodelphax striatellus and Tipula sp. occurred significantly in the fallow paddy fields. Among them, occurrences of N. cincticeps and L. striatellus were low from May to June, and that of L. striatellus was high in August and September. In insect natural enemies, Coenagrion sp., Harmonia axyridis, Propylea japonica and Eristalis tenax occurred significantly in the fallow paddy fields.

• Journal of Applied Biological Chemistry
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• v.53 no.2
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• pp.91-98
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• 2010

This study was aimed to investigate the contents of flavonoids and the biological activity of fermented beverage of medical plants, DeulBit (DB). 50 g of Cassia semen (Cassia tora L.), 50 g of Omija (Schisandra chinensis Baillon.), 50 g of Gugija (Lycium chinense Mill), 50g of Menthae herba, 75 g of Chrysanthemum indicum Linne, 25 g of Dioscorea batatas, 5 g of Lindera obtusiloba Blume, 150 g of Polygonatum odoratum, 25 g of Glycyrrhiza uralensis, 25 g of Acanthopanacis cortex, 100 g of green tea (Camellia sinensis), and 100 g of Laminaria japonica was fermented with sucrose ($50.0{\sim}60.0^{\circ}Brix$.) and 0.5% of deep sea water in 10 L of distilled water for six months at room temperature. Total flavonoids contents of DB was calculated to $3.4{\pm}0.5\;{\mu}g/g$ and antioxidative activity of DB was measured by using DPPH radical scavenging and SOD-like activity. DPPH radical scavenging and SOD-like activity of DB was 96% and 29% at 100% of DB, respectively. In addition, DB indicated about 88% and 66% of the xanthine oxidase and angiotensin converting enzyme inhibitory activities at 1% and 10% of DB, respectively and showed fibrinolytic activity. Nitric oxide (NO) synthesis was increased to 15 times by addition of DB. In addition, NO productions of the macrophages RAW264.7 cells stimulated with lipopolysaccharide (LPS) were reduced to 40.4% by addition of DB. These results suggested that DB is significant role for antioxidative and fibrinolytic activity, and have the strong xanthine oxidase and angiotensin converting enzyme inhibitory activities.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.3
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• pp.205-211
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• 2005

This study was carried out to construct cold-tolerance characteristics. The RILs were developed from progenies of a cross between cold-susceptible Tongil-type rice variety, Milyang 23 and cold-tolerant Japonica rice variety, Stejaree 45 by single seed descent methods. The 175 RILs $(F_8)$ were evaluated for cold tolerance traits by field screening under cold-water irrigation. Frequency distribution of RILs in leaf discoloration, heading delay, culm length reduction and number of spikelets reduction displayed nearly normal distributions with transgressive segregations to either side of parents, while the spikelet fertility reduction and panicle exsertion at low-temperature showed the more or less skewed continuous distribution toward the susceptible parent. Higher heritabilities over $60\%$ were observed in leaf discoloration, spikelet fertility reduction, panicle exsertion, while relatively lower heritabilities less than $40\%$ were observed in culm length reduction, number of spikelets reduction and grain yield reduction. Some cold-tolerance RILs were selected effectively by cold water irrigation, which are expected to be good materials in breeding program for cold tolerance.

• Journal of Applied Biological Chemistry
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• v.61 no.1
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• pp.9-15
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• 2018

We analyzed the antioxidant and anti-wrinkle activities of berberine, isolated from dried rhizome of Coptis japonica Makino, to determine its cosmetic potential. We performed the 3-[4,5-dimethylthiazol]-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay to evaluate the toxicity of the berberine. We also measured the ROS and hyaluronic acid production, and expression of MMP-2, MMP-9, TIMP-1, TIMP-2, and tumor necrosis factor-alpha ($TNF-{\alpha}$) to evaluate the antioxidant and anti-wrinkle activities of berberine, respectively. The cytotoxicity of ultraviolet light, in presence of berberine, was measured by the MTT assay using CCD-986sk fibroblasts, and no cytotoxicity was observed at concentrations less than $25{\mu}g/mL$. We also found that berberine decreased ROS production in a concentration-dependent manner and promoted the synthesis of hyaluronic acid. Further, berberine reduced the protein levels and mRNA expression of MMP-2 and MMP-9, which are associated with wrinkle formation, and increased the expression of TIMP-1 and TIMP-2. In addition, the inhibitory effect of berberine on $TNF-{\alpha}$, known as pro-inflammatory cytokine, was inhibited by $TNF-{\alpha}$ gene in a concentration-dependent manner. These results suggest that berberine holds cosmetic value owing to its antioxidant activity, by inhibiting ROS production and anti-wrinkle activity by reducing MMP-2 and MMP-9 and increasing TIMP-1 and TIMP-2 expression.

• Korean Journal of Weed Science
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• v.5 no.2
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• pp.211-218
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• 1985

To establish the weed control in transplanted bed of Pinus koraiensis and Larix leptolepis, alachlor, simazine, terbutryn, pendimethalin, oxyfluorfen and amitrol/2,4-D/methabenthiazuron (ustinex) were used by soil treatment and foliage application at 7days after transplantion of seedling. From the of soil treatment, grasses such as Dimeria ornithopoda, Alopecurus aequalis, Agropyron tsukushiense and Setaria verticillata were effectively controVed by 85 to 90% at the respective recommended rate of oxyfluorfen, alachlor and terbutryn. At the application rate of recommentation simazine, oxyfluorfen, pendimethalin and terbutryn respectively controlled 70 to 80% of broad-leaved weeds such as Erigeron annuus, Portulaca olearcea, Cerastium arvense, Capsella bursa-pastoris, Commelina commuis, Chenopodium hybridum and Stellaria alsine. Ustinex and oxyfluorfen were very effective for the control of perennials such as Artemisia princeps and Calystegia japonica. Initial sympton of phytotoxicity and decrease of growth in P. koreaiensis and L. leptolepis were not found by soil treatment and those in P. koraiensis was not shown by foliage application of all tested herbicides. But L. leptolepis foliage-applied with ustinex, oxyfluorfen, terbutryn and pendimethalin was great in early phytotoxicity and severe in growth inhibition.

• Korean Journal of Agricultural Science
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• v.6 no.2
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• pp.134-147
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• 1979

Mt. Kyeryong(828 meters in height) is located at $36^{\circ}18^{\prime}{\sim}36^{\circ}23^{\prime}$ N.L. and $127^{\circ}10^{\prime}{\sim}127^{\circ}17^{\prime}$ E.L., and also occupies three districts: Gongju-Gun, Nonsan-Gun, Daedeok-Gun. Authors surveyed the flora of the areas ten times from May to October, 1979. Variability of floras is rather high and dominant species are such as Pinus densiflora, Quercus mongolica, Quercus serrata, Quercus variabilis, Lindera erythrocarpa, Carpinus cordata, Acer pseudo-sieboldianum var. koreanum, Strax japonica, Sapium japonicum, Euonymus oxyphyllus, Sorbus alnifolia, Stephanandra incisa, Weigela subsessilis, Rhododendron mutcronulatum, Lespedeza bicolor, Sasa borealis, Celastrus orbiculatus, Arundinella hirta, Miscanthus sinensis var. purpurascans, and so on. There are 105 families, 355 genera, 679 species. According to the use of floras, they can be classified as follows; 69 familia and 287 species for edible source, 83 familia and 227 species of medicinal, 60 familia and 253 species for forage, 44 familia and 132 species for ornamental and 12 familia and 39 species for timber source.