• The Plant Pathology Journal
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• v.16 no.6
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• pp.297-305
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• 2000

Weeds are widely grown in the field and are infected by many viruses. A survey was conducted to identify viruses infecting weeds in Korea. Virus-infected weed samples including Rorippa indica (L.) Hiern, R. islandica (Oed.) Bord, Crepidiastrum denticulatum (Houtt.) Pak & Kawanno, Achyranthes japonica (Miq.) Nakai, and Chrysanthemum boreale (Makino) Makino were collected in Kyonggi Province. These weeds were grown in the greenhouse and were isolated on 10 test plants. Several virus isolates were isolated fron infected tissues and were further studied by host range assay, serological test, electron microscopy (EM), reverse transcription-polymerase chain reaction (RT-PCR) and sequencing. Each isolated virus strain was mechanically transmitted to weeds and various hosts including Nicotiana spp., Brassica spp., Vigna unguiculata, Capsicum annuum, and Cucumis sativus and showed systemic mosaic, vein clearing, necrosis, mottle, malformation, chlorosis, and/or death of host plants in some cases. Each virus was then purified using infected leaves and observed by EM. From these results three viruses were isolated and identified as Turnip mosaic virus (TuMV), Broad bean wilt virus (BBWV), and Cucumber mosaic virus (CMV). RT-PCR using virus-specific oligonucleotide primers and the cloning were conducted to determine the nucleotide sequences of coat proteins of the three viruses their amino acid sequence were deduced. The amino acid sequence homologies were about 92.7 to 99.7%, 96.2 to 97.7%, and 93.9 to 98.6% to other reported TuMV, BBWV, and CMV strains, respectively. These results suggest that many weeds may serve as primary inoculum source of diseases caused by TuMV, BBWV, CMV and that the management of these viral diseases can be achieved through weed control.

• Journal of Korean Society on Water Environment
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• v.21 no.2
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• pp.169-175
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• 2005

This study was carried out to investigate the effects of irrigation water pollution on the yield and grain quality of rice. It acquires fundamental data to set up water quality standards for irrigation and produce agricultural safety products. The correlations of BOD, T-N, T-P, pH, ECw of the irrigation water with yield, grain appearance quality, and the protein content were evaluated. The field and pot experiments were conducted by using Japonica of Oryza sativa L. during 2 years. BOD concentration in irrigation water effects strongly on grain appearance quality and yield of rice. T-N in irrigation water has strong effect on the yield, appearance, and quality of rice. T-P concentration in irrigation water have not any correlation with yield and quality of rice. pH showed strongly negative correlation with maturity ratio(MTR), 1000 grain weight(TGW), and yield of rice(YLD) as r=-0.803~-0.828(p<0.001) and have no effect on the appearance quality of rice. $EC_w$ indicating salt content showed strongly negative correlation with MTR, TGW, number of grains per panicles(NGP), and number of panicles per unit area(NPM) as r=-0.759~-0.798, and with YLD as -0.753.

• Applied Biological Chemistry
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• v.33 no.2
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• pp.120-124
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• 1990

The occurrence of falcarinol(n-heptadeca-1, 9-dien-4,6-diyn-3-ol)in the umbelliferous plants such as Ladebouriella seseloides WOLFF., Cnidium officinale MAKINO, Foeniculum vulgare GAERTNER, Torilis japonica(HOUTT.) DC. Bupleurum falcatum L.,Angelica gigas NAKAI, Oenanthe javanica (BL.) DC. and Daucus carota var. sativa DC. was investigated using gas chromatography/mass spectrometry(GC/MS). Falcarinol was found in the root of Ledebouriella seseloides, the stem and the root of Oenanthe javanica, and the root of Daucus carota var. sativa in which its contents were $1,055\;{\mu}g/g$, $289\;{\mu}g/g$, $179\;{\mu}g/g$, and $212\;{\mu}g/g$, respectively, while it was not found in the other plants.

• Journal of the East Asian Society of Dietary Life
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• v.15 no.4
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• pp.419-430
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• 2005

In this study, to prove the effect of soybeans's electron donating ability and its addition on eel gouem, storage and sensory characteristics were examined. During the storage period, pH values of all gouem samples were decreased with initial increase except the control. BSB $3\%$ sample had the lowest pH value and YSB $10\%$ sample was the highest 6.71 in pH value at first storage day. The electron donating ability of soybeans were increased with the amount of addition. The electron donating ability of SBSB 20g sample was the highest, which was 2 ${\~}$ 3 times higher than that of YSB sample. L values of all the gouem samples had a tendency to decrease according to the storage period and amount of addition. All samples' a values were increased throughout the storage period, while b values decreased except the control and GNG sample. TBA and VBN values of all the samples were up and down throughout the storage period SBSB $10\%$ sample had the lowest in TBA value. The electron donating ability of all soybean samples and TBA value of eel gouem samples showed a negative correlation. Total plate count of bacteria was increased rapidly for initial storage period, but all samples showed a tendency of slow growth after the 8th day of storage. During all storage days, SBSB, BSB samples had a similar tendency of low total plate count growth. GNG $10\%$ sample showed the lowest in total plate count of bacteria at sixteenth day of storage. Sensory scores of all samples decreased with storage. For all the items of the sensory evaluation, SBSB $5\%$ sample got the highest score among all eel gouem samples. GNGSB sample showed the lowest in TBA value and total plate count of bacteria. And in the sensory evaluation, GNGSB sample got the highest scores for several items.

• Korean Journal of Environment and Ecology
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• v.29 no.3
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• pp.401-409
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• 2015

The fish community and introduced fish were surveyed at eight stations of Lake Soyang from March to October, 2013. A total of 41 species of twelve families were collected from the survey stations and among them, two species: Hemibarbus mylodon and Siniperca scherzeri (albino type) were identified as natural monument. There were fifteen Korean endemic species (36.6 %) including Acheilognathus yamatsutae. When compared with previous data (2003), A. yamatsutae, Pseudorasbora parva, Sarcocheilichthys nigripinnis morii, Sarcocheilichthys variegatus wakiyae, Orthrias nudus, Leiocassisuss ussuriensis, Oncorhynchus mykiss, and Micropterus salmoides appeared new in the lake. Dominant species were Hypomesus olidus (St. 1, 3, 6, 7, 8), Lepomis macrochirus (St. 2, 4) and Zacco platypus (St. 5). Of the 12 introduced species fishes in Lake Soyang, Anguilla japonica, Hemiculter eigenmanni, H. nipponensis, Rhinogobius giurinus and Tridentiger brevispinis were introduced and released from other native river water zones in Korea but Cyprinus carpio (Israeli type), Carassius cuvieri, O. mykiss, Ictalurus puntatus, L. macrochirus, and M. salmoides originated from foreign countries.

• Restorative Dentistry and Endodontics
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• v.22 no.2
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• pp.710-719
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• 1997

Aucubin, an iridoid glucoside, which is isolated from Aucuba japonica, has some biological effects. This study was to investigate the effect of aucubin on the remainig pulp tissues after pulpotomy. Mongrel dog's coronal pulps were mechanically exposed with a sterile round bur and excised with sterile sharp excarvator. After bleeding was controlled, in control group, $Ca(OH)_2$ powder was applied on the remaining pulps and the cavities were sealed with Z.O.E. cement. In experimental group 1, mixed powder with $Ca(OH)_2$ and aucubin(l : 1 by weight) was applied on the pulpotomized pulp surfaces. After the cavities were covered with sterile aluminum foil, they were sealed with Z.O.E. cement. In experimental group 2, only aucubin powder was applied on the remaining pulps and then they were treated the same as experimental group 1. In the all groups, the pulps were histopathologically observed by light microscope at the time intervals of 1, 2 and 4 weeks after experiment. The results were as follows : 1. In control and experimental groups, mild vascular congestion and bleeding were found in most of the specimens. Less inflammatory infiltration was observed in experimental groups than in control group. 2. Dentin bridge formation was found after 1 week at both control and experimental group 1. Dentin birdge had discontinuous osteodentin like appearance or contained some dentin chips. In experimental group 2, dentin bridge was not seen. 3. The coagulation necrosis layer on the remaining pulp tissues was seen in all groups. In experimental group 2, the thickest layer was observed. And in control group, coagulation necrosis layer was similar as in experimental group 1.

• Journal of the Korea Organic Resources Recycling Association
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• v.10 no.2
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• pp.140-146
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• 2002

본 시험은 채종유박과 미강유박을 주원료로 하고 질소, 인산, 칼리의 함량이 7.23%이고 중금속이 거의 없이 개발된 유기질비료(크럼블-80)를 공시비료로 기비와 추비시비 방식의 포장시험을 통하여 토양과 한국잔디의 생육에 미치는 영향과 시비효율을 구명하여 올바른 시비방법 및 시비량을 제시하고자 2000년 4월부터 12월까지 경기도 용인시 소재 태광컨트리클럽 증식포(기비시험)와 경기도 파주시 소재 서서울컨트리클럽 10홀 티(추비시험)에서 실시하였다. 기비시험 결과, 엽록소 측정결과의 처리효과는 무처리구가 100일 때 관행구 117%, 관행구+크럼블-80 $150g/m^2$ 123%, 크럼블-80 $150g/m^2$ 115%, 크럼블-80 $300g/m^2$ 119%, 크럼블-80 $75g/m^2$ 102%로 효과가 있는 것으로 나타났다. 유기질비료인 크럼블-80은 시비량에 따라 엽록소 함량이 약간 증가되었으나 NPK와 혼합처리구에서 가장 높은 것은 공시비료의 단독처리보다는 복합비료와 혼용하는 것이 더 효과적인 것으로 나타났다. 추비시험 결과, 잔디생육기간 중 잔디건물중을 조사한 결과는 크럼블-80의 시용량이 많을수록 잔디건물중도 증가하였으며, 처리구에 따라 무처리구보다 5~45(%) 정도의 증수효과가 있는 것으로 나타났다. 그러나 엽록소함량은 처리구가 무처리구보다는 높았으나 골프코스에 필요한 녹색유지에는 부족하므로 화학비료와의 혼용시비가 필요하다고 판단된다. 본 시험결과로 볼 때 시험에 사용된 공시비료는 골프코스 잔디관리에 적합한 비료로 판단된다. 시험결과를 토대로 한 권장시비량은 기비시비의 경우 잔디식재 전에 공시비료 $300g/m^2$를 표토 10cm와 잘 혼합하여 시비하되, 추비시비는 공시비료와 화학비료와의 혼용시비가 필요하다고 판단된다.

• Journal of Microbiology and Biotechnology
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• v.22 no.6
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• pp.838-848
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• 2012

Orally administered herbal glycosides are metabolized to their hydrophobic compounds by intestinal microflora in the intestine of animals and human, not liver enzymes, and absorbed from the intestine to the blood. Of these metabolites, some, such as quercetin and kaempherol, are mutagenic. The fecal bacterial enzyme fraction (fecalase) of human or animals has been used for measuring the mutagenicity of dietary glycosides. However, the fecalase activity between individuals is significantly different and its preparation is laborious and odious. Therefore, we developed a fecal microbial enzyme mix (FM) usable in the Ames test to remediate the fluctuated reaction system activating natural glycosides to mutagens. We selected, cultured, and mixed 4 bacteria highly producing glycosidase activities based on a cell-free extract of feces (fecalase) from 100 healthy Korean volunteers. When the mutagenicities of rutin and methanol extract of the flos of Sophora japonica L. (SFME), of which the major constituent is rutin, towards Salmonella typhimurium strains TA 98, 100, 102, 1,535, and 1,537 were tested using FM and/or S9 mix, these agents were potently mutagenic. These mutagenicities using FM were not significantly different compared with those using Korean fecalase. SFME and rutin were potently mutagenic in the test when these were treated with fecalase or FM in the presence of S9 mix, followed by those treated with S9 mix alone and those with fecalase or FM. Freeze-dried FM was more stable in storage than fecalase. Based on these findings, FM could be usable instead of human fecalase in the Ames test.

• Journal of Korean Society of Forest Science
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• v.86 no.4
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• pp.422-429
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• 1997

This study was conducted to maintain the productivity of forest production and byproducts production of natural forest. Distribution of wild edible herb species and correlations between wild edible herb species and forest-type, and site factors were surveyed on national forest in Changjeon-ni, Pyungchang-gun, Kang-won-do. Ligularia fischeri was distributed mainly on cutting sites or meadow or Quercus mongolica forest, Pimpinella brachycarpa did on broadleaved mixed forest, and Synurus deltoides did cutting sites or meadow. On conifer plantations major wild edible herb species were not distributed. Pimpinella brachycarpa and Ligularia fischera were distributed mainly on the site of wet soil-moisture condition, Codonopsis lanceolata, Saussurea grandifolia and Synurus deltoides were distributed randomly. The distribution of Ligularia fischeri and Pimpinella brachycarpa were significantly different between slope directions, but those of Codonopsis lanceolata, Saussurea grandifolia and Synurus deltoides were not significant. Ligularia fischeri and Pimpinella brachycarpa were distributed more frequently in north directed slope than the other. Highly positive correlations were proved Ligularia fischeri and Pimpinella brachycarpa, Aconitum jaluense and Veratrum patulum; Pimpinella brachycarpa and Smilacina japonica, Aconitum jaluense and Veratrum patulum.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.5
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• pp.741-746
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• 1996

Antibacterial activities of the bamboo(Pseudosasa japonin Makino) leaves extract on lactic acid bacteria related to Dongchimi fermentation were investigated. The bamboo leaves were extracted with 70% ethanol, fractionated it with 4 solvents which have a different polarity. Total viable count was very similar to those obtained by lactic acid bacteria during fermentation of Dongchimi. Its maximum counts were (equation omitted) 10$^{7}$ CFU/m1 at 8 days of fermention. Lactic acid bacteria isolated during Dongchimi fermentation were identified as Leuconostoc species(BK-2, BK-07, BK-22), Lactobaillus species (BK-05, BK-08) and Streptococcus species(BK-15) 70% ethanol extract of the bamboo leaves showed antibacterial activity against strain BK-02, BK-07, BK-lS and BK-22. Especially, strain BK-22 was more effective to the extracted substance. Among the each fractions, 70% ethanol and diethyl ether had the strongest antibacterial activity against strain BK-22 and BK-07, respectively. The two fractions also showed similar activities against all of the test strains.