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Purification and Characterization of Antioxidative Peptides from Enzymatic Hydrolysate of Cod Teiset Protein (대구고니 단백질의 효소적 가수분해물로부터 항산화성 펩타이드의 분리${\cdot}$정제 및 특성)

  • KIM Se-Kwon;CHOI Yong-Ri;PARK Pyo-Jam;CHOI Jeoung-Ho;MOON Sung-Hoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.3
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    • pp.198-204
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    • 2000
  • In order to utilize by-products which would normally be discarded in marine processing plants, cod teiset protein was hydrolyzed and antioxidative actiTity of the hydrolysate was investigated. AntioxidatiTe peptide was isolated using ultrafiltration membrane, ion-exchange chromatography on a SP-Sephadex C-25 column, gel filtration on a Sephadex G-15 column, high performance liquid chromatography on an ODS column, and capillary electrophoresis chromatography. Antioxidative activities of the cod teiset hydrolysate were compared with ${\alpha}-tocopherol$, one of the commercial antioxidant. The hydrolysate passed through a membrane with molecular weight cut-off (MWCO) 1 kDa was shown the strongest antioxidative activity, and the activity was higher $10{\%}$ as compared with ${\alpha}-tocopherol$. In addition, the peptide isolated by ion-exchange chromatography, gel filtration, and HPLC, respectively, was higher $53{\%}$ as compared with ${\alpha}-tocopherol$, and the amino acid sequence was Ser-Asn-Pro-Glu-Trp-Ser-Trp-Asn.

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A Study of Costumes of the 18th Century, Appearing in Genre Paintings from the King Young-Cho Period to the King Jung-Cho Period: Focused upon the Works of Focused upon the Works of Mung-Hyun Oho, Yong Yun, In-Sang Lee, Hee-Eon Kang (영ㆍ정조 시대의 속화에 나타난 18C 복식에 관한 연구 -오명현, 윤용, 이인상, 강희언, 작품을 중심으로-)

  • 최은주
    • The Research Journal of the Costume Culture
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    • v.12 no.5
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    • pp.859-879
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    • 2004
  • As a result of research, the characteristics of the general costumes from the king Young-Cho period to the king Jung-Cho period in Genre Paintings of Mung-Hyun Oho, Yong Yun, In-Sang Lee, Hee-Eon Kang follows. First, the typical man wore his hair in a topknot(sangtu), and put on 'Bung-gu-ji', 'Lip', or a scarf on his head. The length of the 'Jeogori'(Korean traditional jacket) was long enough to cover the waist. Dress for work had side slits, and had half length sleeve Jeogori, and short pants looked like 'Jam-bang-i'. They went barefoot and wore 'Jipsin'(Korean traditional straw shoes). Dress for outdoor was 'Po' that knot at front of chest by band. 'Baji'(slacks) were with knot below knee, worn 'Hang-jun'(ankle band) and the width of slacks was suitable. They were 'Beoseon'(Korean traditional socks) and shoes. Second those in the upperc1ass and those in the military put on 'Mang-geon', 'Gat', 'Sa-bang-gan', 'Tang-geon', 'Bok-geon' on their head on a topknot. Most of them wore 'So-chang-i', 'Jung-chi-mag' or 'Do-po'. The length of Jeogori covered the waist or the hip and were tied with 'Go-rum'(ribbon). Baji was tied with Hang-jun and 'Dae-nim'. The waist of the slacks were tied with a dark colored waist-band and folded down their waist of slacks. They wore white color Beoseon and 'Hye' or dark color leather shoes. They wore 'Sup'(assistant of arm) for bow. It showed the lifestyle of the 18C with fan, 'Be-ru', 'Mug', 'Yun-jug', teacup, pot, etc. Third, child's hair was short or knotted to the back of the head. The length of Jeogori reached waist line, Git of Jeogori was 'Dunggurai-Kit'(shape of round) and other style Jeogori, which reached the hip line, had side-slit. Baji was tied with Dae-nim, and the width of the slacks is suitable. They hang 'Yum-nang'(Pocket). Final, most women worked outdoors wearing their hair in a high twisted style, or covered it with scarf. They wore Jeogori and 'Chima'(Korean traditional skirts), Bagi. They folded up the sleeves of the Jeogori. And they folded the 'Jambang-i-styled' pants to just above the knees, fastening at the waist. When they wore skirts, they also wore underpants under the skirt that went down to the knees. Most of them went barefoot and wore straw shoes, Jipsin. Through genre paintings, we can understand the ways and forms of our ancestor's clothing. And with our understanding, interest, and passion, we can be familiar with Hanbok in our daily life by succeeding and creating its peculiar style. And then we can promote the globalization of Hanbok.

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A Study on the Performance Improvement of Bar Code Reader for the Automatic Processing of the Mail Items (우편물 자동처리를 위한 바코드 판독기 성능개선에 관한 연구)

  • Park, Moon-Sung;Nam, Yun-Seok;Kim, Hey-Kyu
    • Proceedings of the Korea Information Processing Society Conference
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    • 2001.04b
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    • pp.731-734
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    • 2001
  • 우편물을 집배원이 배달하는 순서로 자동구분 처리하기 위한 요소기술 줌에서 4-state 바코드 시스템이 개발되고 있으며 우편번호, 배달순서코드, 고객정보 등이 적용될 예정이다. 기존의 고객 바코드 판독 시스템은 우편물상의 바코드 심볼로지가 존재하는 판독대상 영역의 기울기가 ${\pm}4.47^{\circ}$ 이하이고, 심볼의 훼손과 잡영이 없을 경우에 $79{\sim}100msec(35,000{\sim}45,000$통/시간)의 속도로 자동 구분 정보가 판독된다. 본 논문에서는 판독범위 및 판독성능을 개선을 위하여 CCD(Charge Coupled Device) 센서로부터 획득된 이미지상에서 존재하는 심볼로지 정보의 고속판독 방법을 제시한 것이다. 이 판독방법은 그레이(gray) 이미지 바탕면의 경계값(threshold) 기울기 분포를 기준으로 2개의 경계값을 설정하여 판독대상 정보를 획득하였다. 또한, 4-state 바코드 심볼로지의 존재 가능성 영역만을 탐색하고, 판독대상 영역에서 트래커(tracker)를 탐색하여 심볼로지의 기울기값, 심볼로지 경계값, 심볼위치 좌표값을 생성한 후 심볼값이 판독한 것이다. 판독시험 결과는 판독대상 영역의 심볼로지가 ${\pm}45^{\circ}$ 기울어지고, 잡영이 존재할 경우에도 $30{\sim}60msec(58,000{\sim}l16,000$통/시간) 이내에 판독되었다. 우편물 자동구분용 바코드 판독기로써 적용될 경우에 판독속도가 평균 57.25% 이상 개선되고, 판독범위의 확장으로 0.2%의 기계적인 오류(이송과정예서의 Jam 발생 비율)를 제외할 경우에 거의 99.8% 우편물을 판독하여 자동구분 처리할 수 있게 될 것으로 기대된다.onebook 엑세스 모들(Server Phonebook Access Module)로 구성되어 있다.외 보다 높았다(I/O ratio 2.5). BTEX의 상대적 함량도 실내가 실외보다 높아 실내에도 발생원이 있음을 암시하고 있다. 자료 분석결과 유치원 실내의 벤젠은 실외로부터 유입되고 있었고, 톨루엔, 에틸벤젠, 크실렌은 실외뿐 아니라 실내에서도 발생하고 있었다. 정량한 8개 화합물 각각과 총 휘발성 유기화합물의 스피어만 상관계수는 벤젠을 제외하고는 모두 유의하였다. 이중 톨루엔과 크실렌은 총 휘발성 유기화합물과 좋은 상관성 (톨루엔 0.76, 크실렌, 0.87)을 나타내었다. 이 연구는 톨루엔과 크실렌이 총 휘발성 유기화합물의 좋은 지표를 사용될 있고, 톨루엔, 에틸벤젠, 크실렌 등 많은 휘발성 유기화합물의 발생원은 실외뿐 아니라 실내에도 있음을 나타내고 있다.>10)의 $[^{18}F]F_2$를 얻었다. 결론: $^{18}O(p,n)^{18}F$ 핵반응을 이용하여 친전자성 방사성동위원소 $[^{18}F]F_2$를 생산하였다. 표적 챔버는 알루미늄으로 제작하였으며 본 연구에서 연구된 $[^{18}F]F_2$가스는 친핵성 치환반응으로 방사성동위원소를 도입하기 어려운 다양한 방사성의 약품개발에 유용하게 이용될 수 있을 것이다.었으나 움직임 보정 후 영상을 이용하여 비교한 경우, 결합능 변화가 선조체 영역에서 국한되어 나타나며 그 유의성이 움직임 보정 전에 비하여 낮음을 알 수 있었다. 결론: 뇌활성화 과제 수행시에 동반되는 피험자의 머리 움직임에 의하여 도파민 유리가 과대평가되었으며 이는 이 연구에서 제안한 영상정합을 이용한 움직임 보정기법에 의해서 개선되었다. 답이 없는 문제, 문제 만

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Studies on the Rooting Ability of Cutting in Elder Berry(Sambucus canadiensis) (황금(黃金)포도나무(Elder berry)의 삽목시험(揷木試驗))

  • Park, Kyo Soo
    • Journal of Korean Society of Forest Science
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    • v.40 no.1
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    • pp.43-50
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    • 1978
  • The elder berry was known to the rich in natural food colour resources and used in as for making wine, confectionary, perfumes, natural food colour, making elder berry juice, jelly, jam and medicinal properties or oils. In the present study, wish was to find the effect of various factors on the success of the vegetative propagation of elder berry by means cutting in the exposed field and green house was carried out and those obtained results can be summarized as follows. 1. Cuttings with dormant cutting stocs in the polyethylen house with heating and water mist spray resulting 100 percent survival. And temperature and the relative humidity in which fraim during the cutting season were around $20{\sim}25^{\circ}C$ and 70-90% respectively and in case as more significant other of the 1% Level. 2. With five varieties tested, resulting 93.8 percent survival, The F. value is not significant. 3. With four organs of cutting stock tested resulting 57.5 percent survival on the cuttings with two knodes of dormant cutting stock served as better cutting stock than others. The F. value is more significantly 1% Level. 4. Dormand bud served as possible cutting stock was found to be 17.66 survival percentage. 5. Both earlier and later stage of germinated Leaves with soft wood cutting stock poor cuttings, and the degree of development of 15th June cutting stock was optimum stage on the principal factor governing the success of cutting in the soft wood cutting showing 54% survival. The F. value is more 1% Level significant.

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Calcium Absorption Acceleration Effect on Phosphorylated and Non-phosphorylated Peptides from Hoki (Johnius Belengeri) Frame (민태 Frame 단백질 유래의 인산화 및 비인산화 펩타이드가 체내 칼슘 흡수에 미치는 영향)

  • KIM Se-Kwon;JEON You-Jin;BYUN Hee-Guk;PARK Pyo-Jam;KIM Gyu-Hyung;CHOI Yong-Ri;LEE Yeon-Sook
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.6
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    • pp.713-717
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    • 1999
  • In order to utilize protein hydrolysate produced from hoki (Johnius Belengeri) frame among many different fish processing wastes, hoki frame peptide (PHFP) and phosphorylated hoki frame peptide (PHFP) were prepared, and their calcium absorption accelerating effects were investigated in comparison to control and casein phosphopeptide (CPP). In in vitro experiment, HFP and PHFP inhibited calcium phosphate formation as high as 1.5-fold and 2.5-fold, respectively, comparing to control, In addition, the inhibition rate of calcium phosphate precipitation as increasing concentrations of HFP and PHFP was risen and was similar to that of CPP at 2.0 mg/ml of PHFP concentration, In in vivo experiment using the rats, the groups fed HFP and PHFP indicated significantly increased calcium content in the femur. In particular, the calcium content in the small intestine of the rat fed PHFP was higher than that of control group by approximately $60\%$.

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Seroreactivity to Q Fever Among Slaughterhouse Workers in South Korea

  • Chu, Hyuk;Yoo, Seok-Ju;Hwang, Kyu-Jam;Lim, Hyun-Sul;Lee, Kwan;Park, Mi-Yeoun
    • Journal of Preventive Medicine and Public Health
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    • v.50 no.3
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    • pp.195-200
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    • 2017
  • Objectives: Q fever is a zoonotic disease that occurs worldwide; however, little is known about its prevalence in South Korea. We attempted to determine the prevalence of Q fever seroreactivity among Korean slaughterhouse workers and the risk factors for seroreactivity according to the type of work. Methods: The study was conducted among 1503 workers at a total of 73 slaughterhouses and 62 residual-product disposal plants. During the study period, sites were visited and surveys were administered to employees involved in slaughterhouse work, and serological tests were performed on blood samples by indirect immunofluorescence assays. Serological samples were grouped by job classification into those of slaughter workers, residual-product handlers, inspectors and inspection assistants, and grading testers and testing assistants. Employee risk factors were analyzed according to the type of work. Results: Out of 1481 study subjects who provided a blood sample, 151 (10.2%) showed reactive antibodies. When these results were analyzed in accordance with the type of work, the result of slaughter workers (11.3%) was similar to the result of residual-product handlers (11.4%), and the result of inspectors and assistants (5.3%) was similar to the result of grading testers and assistants (5.4%). Among those who answered in the affirmative to the survey question, "Has there been frequent contact between cattle blood and your mouth while working?" the proportions were 13.4 and 4.6%, respectively, and this was identified as a risk factor that significantly varied between job categories among slaughterhouse workers. Conclusions: This study found a Q fever seroreactivity rate of 10.2% for slaughterhouse workers, who are known to be a high-risk population. Contact with cattle blood around the mouth while working was the differential risk factor between job categories among slaughterhouse workers.

Pulsed Field Gel Electrophoresis Profile of Erythromycin-Clindamycin Resistant Streptococcus pyogenes Isolated in Korea (국내분리 Erythromycin-Clindamycin 내성 Streptococcus pyogenes에 대한 Pulsed Field Gel Electrophoresis 양상 분석)

  • Lee, Young-Hee;Hwang, Kyu-Jam;Lee, Kwang-Jun;Park, Kang-Soo;Bae, Song-Mee;Sung, Hwa-Young;Kim, Ki-Sang;Lee, Chong-Sam
    • The Journal of the Korean Society for Microbiology
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    • v.35 no.2
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    • pp.171-180
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    • 2000
  • Ninety two strains of Streptococcus pyogenes were isolated from patients with pharyngitis, scarlet fever, skin infection, and invasive streptococcal infections in Seoul, Korea from January to December, 1998. All isolates were epidemiologically characterized by T protein serotype, and serum opacity factor (OF) detection to phenotypes. To analyze the genetic relationship, fifty two isolates including 32 erythromycin-clindamycin (Em-Cm) resistant strains, 20 antimicrobial susceptible strains were attempted to the pulsed-field gel electrophoresis (PFGE). T protein serotype showed 16 kinds in distribution including T12 and T4. Among the total isolates, 40 strains (43.5%) belonged to the T12 serotype and twenty strains (21.7%) to T4 serotype. On the other hand, when infection aspect of S. pyogenes isolates were analysed by T serotype distribution, T12 type was predominant for pharyngitidis which contributed to 21 strains (53%) and for skin infection isolates which contributed to 11 strains (28%), respectively. In case of T4 type, it was the most predominant pharyngitidis isolates which contributed to 8 strains (40%). In T serotype distribution of Em-Cm resistant strains, 27 strains (84%) of the thirty two showed T12 serotype. In minimum inhibitory concentration (MIC) values of Em-Cm resistance isolates, thirty two isolates showed resistant to erythromycin 27 strains (84%), had high MIC of >$128\;{\mu}g/ml$. And also to clindamycin, twenty two strains (69%) had high MIC of >$128\;{\mu}g/ml$. When OF detection of Em-Cm resistance of S. pyogenes isolates were analyzed by T serotype distribution, T12 serotype isolates revealed that all of the isolates except one strain were OF negative. In PFGE profile analysis to Em-Cm resistance isolates, of the twenty seven, Em-Cm resistance of T12 serotype isolates, 26 strains showed identical PFGE profile and all of these isolates revealed that OF negative. Eighty four percent of Em-Cm resistance S. pyogenes isolates had identical phenotype and PFGE profile. These results strongly suggested that the Em-Cm resistant S. pyogenes isolates from Seoul area showed close genetic correlation and PFGE could be available tool for molecular epidemiology.

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Seasonal Variation of Larval Fish Community in Jinhae Bay, Korea (진해만 자어 군집의 계절 변동)

  • Moon, Seong Yong;Lee, Jeong-Hoon;Choi, Jung-Hwa;Ji, Hwan Sung;Yoo, Joon-Taek;Kim, Jung-Nyun;Im, Yang Jae
    • Korean Journal of Environmental Biology
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    • v.36 no.2
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    • pp.140-149
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    • 2018
  • Seasonal variations in the community structure of larval fish assemblage in Jinhae Bay were investigated in February, May, August and November of 2015, and in the same months in 2016. During the study period, a total of 28 larvae species belonging to 24 families were collected. The dominant species were Clupea pallasii, Liparis tanakae, Callionymidae sp., Sillago japonica, Ernogrammus hexagrammus and Engraulis japonica. These six species accounted for 77.6% of the total number of larvae during the survey period. The larvae of C. pallasii and L. tanakae were exclusively caught in February 2015 and 2016, while those of Apogon lineatus and Sillago japonica were exclusively caught in August 2015 and 2016. Overall, species diversity of the fish larvae was highest in February and August, although it was relatively low in May. The results of the non-metric multidimensional scaling (nMDS) analysis using the number of individual larval fish showed that they are divided into four seasonal groups. Our results showed that the community of larval fish in Jinhae Bay was affected by seasonal changes in temperature and the emergence of spawning fish species. Additionally, we suggest that the high abundance of larval fish came from around Jam-do and the northern part of Chilcheon-do; these locations are the main spawning grounds of Jinhae Bay.

Antimicrobial Activity of Pectin hydrolysate and its Preservative Effect (펙틴분해물의 항균특성과 식품보존효과)

  • 박미연;최승태;장동석
    • Journal of Food Hygiene and Safety
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    • v.13 no.2
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    • pp.99-105
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    • 1998
  • Pectin hydrolysate prepared from citrus pectin by enzymatic hydrolysis has antimicrobial activity. The antimicrobial activity was increased by its hydrolyzing rate and it was rapidly increased after 70% hydrolysis of the pectin. The antibacterial activity of pectin hydrolysate against Escherichia coli ATCC 11229 was the strongest at pH 4.9~5.5, but it diminished slightly at neutral pH values. The antibacterial activity of pectin hydrolysate was stronger than those of against molds and yeasts. The growth of bacteria submitted to this test except lactics was completely inhibited for 48 hrs at $35^{\circ}C$ by adding 2.0~3.0% pectin hydrolysate. While the growth of Lactococcus lactis ATCC 19435 and Lactobacillus bulgaricus and Penicillium funiculosum ATCC 11797 were reached about 60~70% compared with those of the controls in the same condition. But there was no significant effect on the growth of the yeasts. The antibacterial effect of pectin hydrolysate was significantly stimulated by addition of glycine, ethanol, sodium ascorbate, sodium chloride and sodium acetate. The shelf life of Kimchi containing 1.0% pectin hydrolysate was prolonged above 15 days at $4^{\circ}C$ than that of its control. In case of whitish bean jam viable cell counts were inhibited about 2 log cycles by 10 days at $25^{\circ}C$. According to these results, author can sincerely suggest that pectin hydrolysate will be used as a natural food preservative for inhibition of common bacterial growth without inhibition of lactics and yeasts.

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GIS and Statistical Techniques used in Korea Urban Expansion Trend Analysis (GIS와 통계기법을 이용한 대한민국 도시확장 패턴분석)

  • Son, Jung-Woo;Jeon, Sung-Woo;Choi, Chul-Uong
    • Journal of Korean Society for Geospatial Information Science
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    • v.17 no.4
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    • pp.13-22
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    • 2009
  • Urban expansion has caused environmental problems, traffic jam and real estate. Trend analysis of Urban expansion is needed for analysis and prediction of the existing problem-solving, urban planning and land use. In this study, We constructed database based on MOE(Minister of environment)'s landcover(1980's, 2000's), 1: 25,000 digital topographical map using of DEM and data of the National Statistical Office for urban and build up expand analysis of South Korea. As a result, The rate of increase in population of Gyeonggi-do, Incheon and Ulsan are high but Jeollanam-do is low. Area of development zone was 2.15 fold increase in comparison with before it. Slope aspect is south or east and urban expansion was increase in district such as Chungcheongnam-do, Gyeonggi-do, Jeollanam-do. Existing road of accessibility was high than development zone. Metropolitan city developre it. In conclusion, we found that South Korea urban expansion was developed from metropolitan city. In natural topographical conditions, the development was progress advantageous zone to disadvantageous zone. Also, we found that population was rapidly increase with new development as the center zone in urban expansion zone.

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