• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.5
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• pp.237-251
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• 2002

The recent progress on metabolic systems engineering was reviewed based on our recent research results in terms of (1) metabolic signal flow diagram approach, (2) metabolic flux analysis (MFA) in particular with intracellular isotopomer distribution using NMR and/or GC-MS, (3) synthesis and optimization of metabolic flux distribution (MFD), (4) modification of MFD by gene manipulation and by controlling culture environment, (5) metabolic control analysis (MCA), (6) design of metabolic regulation structure, and (7) identification of unknown pathways with isotope tracing by NMR. The main characteristics of metabolic engineering is to treat metabolism as a network or entirety instead of individual reactions. The applications were made for poly-3-hydroxybutyrate (PHB) production using Ralstonia eutropha and recombinant Escherichia coli, lactate production by recombinant Saccharomyces cerevisiae, pyruvate production by vitamin auxotrophic yeast Toluropsis glabrata, lysine production using Corynebacterium glutamicum, and energetic analysis of photosynthesic microorganisms such as Cyanobateria. The characteristics of each approach were reviewed with their applications. The approach based on isotope labeling experiments gives reliable and quantitative results for metabolic flux analysis. It should be recognized that the next stage should be toward the investigation of metabolic flux analysis with gene and protein expressions to uncover the metabolic regulation in relation to genetic modification and/ or the change in the culture condition.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.2
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• pp.75.2-75.2
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• 2014

CS molecule as an important tracer for studying inward motions in dense cores is known to be adsorbed onto dusts in cold (T~10K) dense cores, resulting in its significant depletion in the central region of the cores which may hamper a proper study of kinematics stage of star formation. In this study we choose five 'evolved' dense starless cores, L1544, L1552, L1689B, L694-2 and L1197, to investigate how depletion of CS molecule is significant and how the molecule differentiates depending on the evolutional status of the dense cores, by using a rare isotopomer C34S. We performed mapping observations in C34S (J=2-1) and N2H+ (J=1-0) with Nobeyama 45 m telescope, and compared $850{\mu}m$ continuum data as a reference of the density distribution of the dense cores. Our data confirm the claim that CS molecule generally depletes out in the central region in dense starless cores, while N2H+ keeps abundant as they get evolved. All of integrated intensity maps show 'semi-ring-like' depletion holes in CS, and all of abundance radial profiles show decrease toward center. The CS depletion and molecular chemical differentiation seems to depend on the evolutional status in dense cores. The evolved cores shows low abundance at both central and outer regions, implying that in the case of highly evolved cores CS freeze-out occurs over the most area of the cores.