• Parasites, Hosts and Diseases
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• v.51 no.5
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• pp.573-577
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• 2013

A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.

• Transactions of the Korean Society of Mechanical Engineers
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• v.16 no.4
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• pp.765-774
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• 1992

The effect of the interactive flow on convective heat transfer from two vertical isothermal parallel plates have been studied numerically by the finite difference method. The Reynolds number, Grashof number, the relative length, L$_{2}$/L$_{1}$, and the dimensionless plate spacing, b/L$_{1}$ are varied as parameters. In case of outside mean Nusselt number, left outside mean Nusselt numbers show same values as L$_{2}$/L$_{1}$ and b/L$_{1}$ increase, but right outside mean Nusselt numbers decrease as L$_{2}$/L$_{1}$ increases. The inside mean Nusselt numbers are constant at narrow spacings and increase at wide spacings as Grashof numbers increase. The optimun plate spacing on left inside mean Nusselt numbers is b/L$_{1}$=0.4 at Re=100 and b/L$_{1}$=0.3 at Re=200. For the right inside mean Nusselt number, the optimum plate spacings move to the narrow spacing as Reynolds numbers increase and L$_{2}$/L$_{1}$ decrease.

• The Plant Pathology Journal
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• v.35 no.6
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• pp.635-643
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• 2019

To detect Xanthomonas arboricola pv. pruni, a loopmediated isothermal amplification (LAMP) detection method were developed. The LAMP assay was designed to test crude plant tissue without pre-extraction, or heating incubation, and without advanced analysis equipment. The LAMP primers were designed by targeting an ABC transporter ATP-binding protein, this primer set was tested using the genomic DNA of Xanthomonas and non-Xanthomonas strains, and a ladder product was generated from the genomic DNA of X. arboricola pv. pruni strain but not from 12 other Xanthomonas species strains and 6 strains of other genera. The LAMP conditions were checked with the healthy leaves of 31 peach varieties, and no reaction was detected using either the peach leaves or the peach DNA as a template. Furthermore, the high diagnostic accuracy of the LAMP method was confirmed with 13 X. arboricola pv. pruni strains isolated from various regions in Korea, with all samples exhibiting a positive reaction in LAMP assays. In particular, the LAMP method successfully detected the pathogen in diseased peach leaves and fruit in the field, and the LAMP conditions were proven to be a reliable diagnostic method for the specific detection and identification of X. arboricola pv. pruni in peach orchards.

• Korean Journal of Agricultural Science
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• v.47 no.1
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• pp.173-182
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• 2020

Human Astrovirus (HuAstV), known as a waterborne virus, is a group IV positive-sense single-stranded RNA that belongs to Astroviridae. The first outbreak of HuAstV was reported in England in 1975. HuAstV can exist not only among clinical patients but also in various water environments, such as water for agriculture and vegetables. For diagnosis of HuAstV from water samples, a polymerase chain reaction (PCR) system has been developed. However, the PCR-based diagnostic method has problems in field application, such as reaction time, sensitivity and specificity. For this reason, in this study we developed the loop-mediated isothermal amplification assay (LAMP) system, aimed specifically at HuAstV. Three prepared LAMP primer sets were tested by specificity, non-specificity and sensitivity; one LAMP primer set was selected with optimum reaction temperature. The developed LAMP primer set reaction conditions were confirmed at 62℃, and detection sensitivity was 1 fg/μL. In addition, restriction enzyme HaeIII (GG/CC) was introduced to confirm that the LAMP reaction was positive. As a result, selected LAMP primer set was 100 - 1000 times more specific, rapid, and sensitive than conventional-nested PCR methods. For verification of the developed LAMP assay, twenty samples of cDNA from groundwater samples were tested. We expect that the developed LAMP assay will be used to diagnose HuAstV from various samples.

• Transactions of Materials Processing
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• v.14 no.2 s.74
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• pp.126-132
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• 2005

The isothermal forging design of a Ti-6Al-4V wing shape was performed by 3D FE simulation. The design focuses on near-net shape forming by the single stage. The process variables such as the die design, pre-form shape and size, ram speed and forging temperature were investigated. The main design priorities were to minimize forging loads and to distribute strain uniformly in a given forging condition. The FE simulation results for the final process design were compared with the isothermal forging tests. The instability of deformation was evaluated using a processing map based on the dynamic materials model(DMM), including flow stability criteria. Finally, a modified process design for producing a uniform Ti-6Al-4V wing product without forming defects was suggested.

• Journal of Welding and Joining
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• v.24 no.4
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• pp.39-49
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• 2006

The austenite grain growth model in low alloyed steel HAZ without precipitates was proposed by analyzing isothermal grain growth behavior. Steels used in this study were designed to investigate the effect of alloying elements. Meanwhile, a systematic procedure was proposed to prevent inappropriate neglect of initial grain size (D0) and misreading both time exponent and activation energy for isothermal grain growth. It was found that the time exponent was almost constant, irrespectively of temperature and alloying elements, and activation energy increased with the addition of alloying elements. From quantification of the effect of alloying elements on the activation energy, an isothermal grain growth model was presented. Finally, combining with the additivity rule, the austenite grain size in the CGHAZ was predicted.

• Carbon letters
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• v.9 no.4
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• pp.308-315
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• 2008

In the present study, fast isothermal stabilization processes for rayon precursor fabrics were performed at $350^{\circ}C$ and $400^{\circ}C$ within 3 minutes and the chemical and physical characteristics of the stabilized fabrics were investigated. In addition, rayon precursor fabrics were pre-treated with three different phosphorous-based flame retardants and then stabilized. The effect of flame retardants on the chemical composition, thermal shrinkage, weight change, thermal stability and XRD results was examined, comparing with those of the precursor fabrics. The result showed that the stabilization of rayon fabrics was most effective as the stabilization temperature was $350^{\circ}C$, the stabilization time was 3 min, and the pre-treatment with phosphoric acid of 1 vol%. The carbon contents of stabilized rayon fabrics were increased with increasing stabilization temperature and time, whereas the oxygen contents were decreased. Also, it is likely that the pre-treatment with phosphoric acid plays a role in retarding the change of chemical structure of rayon fabric. The XRD result was quite consistent with the result showing the effect of phosphoric acid on the chemical composition, thermal shrinkage and weight reduction of rayon fabric.

• Computers and Concrete
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• v.12 no.1
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• pp.53-64
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• 2013

The curing temperature is known to influence the rate of mechanical properties development of early age concrete. In realistic sites the temperature of concrete is not isothermal $20^{\circ}C$, so the paper measured adiabatic temperature increases of four different concretes to understand heat emission during hydration at early age. The temperature-matching curing schedule in accordance with adiabatic temperature increase is adopted to simulate the situation in real massive concrete. The specimens under temperature-matching curing are subjected to realistic temperature for first few days as well as adiabatic condition. The mechanical properties including compressive strength, splitting strength and modulus of elasticity of concretes cured under both temperature-matching curing and isothermal $20^{\circ}C$ curing are investigated. The results denote that comparing temperature-matching curing with isothermal $20^{\circ}C$ curing, the early age concretes mechanical properties are obviously improved, but the later mechanical properties of concretes with pure Portland and containing silica fume are decreased a little and still increased for concretes containing fly ash and slag. On this basement using an equivalent age approach evaluates mechanical properties of early age concrete in real structures, the model parameters are defined by the compressive strength test, and can predict the compressive strength, splitting strength and elasticity modulus through measuring or calculating by finite element method the concreted temperature at early age, and the method is valid, which is applied in a concrete wall for evaluation of crack risking.

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.2
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• pp.467-478
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• 1994

This paper deals with dynamic behaviors of a free-piston Vuilleumier heat pump system, which are characterized by stroke of each diplacer/stroke ratio, operating frequency and phase angle. Based on the Isothermal Model, basic equations of motion are derived and linearized. In particular, dependence of damping coefficients of the dynamic parameters are taken into account in the formulation, which does not bring additional difficulties in the analysis. In order to investigate effects of design conditions on the dynamic parameters are taken into account in the formulation, which does not bring additional difficulties in the analysis. In order to investigate effects of design conditions on the dynamic characteristics, calculations are performed for the prototype made by Schulz and Thomas and results are qualitatively compared with their data obtained from the analysis as well as the experiment. It appears that they made a mistake in evaluating the hysteresis loss of the gas spring in their analysis. And, the present results show a better agreement with their experimental data than those by their own analysis. Although there are some unresolved aspects such as frequency variations with respect to the mean pressure and the hot space temperature, it is expected that the present analysis may be an effective tool for prediction of dynamics of a free- pistion VM machine at the preliminary design stage.

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.10
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• pp.2586-2594
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• 1995

Two phase flow phenomena are observed in many industrial facilities and make much importance of optimum design for nuclear power plant and various heat exchangers. This experimental study has been investigated the classification of the flow pattern, the local void distribution and convective heat transfer in swirl and non-swirl two phase flow under the isothermal and nonisothermal conditions. The convective heat transfer coefficients in the single phase water flow were measured and compared with the calculated results from the Sieder-Tate correlation. These coefficients were used for comparisons with the two-phase heat transfer coefficients in the flow orientations. The experimental results indicate, that the void probe signal and probability density function of void distribution can used into classify the flow patterns, no significant difference in voidage distribution was observed between isothermal and non-isothermal condition in non-swirl flow, the values of two phase heat transfer coefficients increase when superficial air velocities increase, and the enhancement of the values is observed to be most pronounced at the highest superficial water velocity in non-swirl flow. Also two phase heat transfer coefficients in swirl flow are increased when the twist ratios are decreased.