• 한국식품영양학회지
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• 제6권4호
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• pp.268-275
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• 1993

The whole cells of Actinoplanes missouriensis KCTC 1780 which produce glucose isomerase was immobillized by flocculation method for the effective production of high fructose syrup using packed-bed bioreactor system. Among the flocculation methods used In this study, the glucose Isomerase activity of flocculated cells using 5% polyethylenimmine and 0.2% glutaraldehyde was the highest as 46.3 unit, and the flocculant was 10.3g(wet weight) per 100m1 of broth, and the residual activity was 92.5%. In the batch operation of glucose isomerization using the flocculated cells, the optimum pH, temperature and isomerization ratio were 7.0, 75$^{\circ}C$ and 31%, respectively. The optimum concentration of Mg2+ which was activator on the glucose isomerization of flocculated cells was 0.1M, and glucose isomerase activity was increased by about 40% compared to none of Mg2+. In the packed-bed bioreactor system with 1.2 hour of residence time at 7$0^{\circ}C$, the reaction stability maintained until 96 hour without toss of activity, and the equilibrium was kept up to 120 hours of the operation.

• Mass Spectrometry Letters
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• 제15권2호
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• pp.107-119
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• 2024

We aimed to develop a suitable quantification method for detecting asparagine deamidation and aspartic acid isomerization in peptide mapping using LC-MS. Our assessment of its validity and suitability involved comparing its quantitative findings with those obtained from cation-exchange chromatography and capillary electrophoresis methods. By subjecting trastuzumab to rigorous conditions to induce these modifications, we validated the efficacy of this new analytical method in peptide mapping via LC-MS, evaluating both qualitative and quantitative aspects of asparagine deamidation and aspartic acid isomerization. Our investigation underscored the significance of enzyme selection and the presence of miss-cleaved or non-specific peptides in achieving accurate quantitative results. The experimental results demonstrated a strong correlation with results from cation-exchange chromatography and capillary electrophoresis analyses, confirming the reliability of the LC-MS based peptide mapping approach.

• Journal of Photoscience
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• 제9권2호
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• pp.1-4
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• 2002

Hula-twist is a volume-conserving photoisomerization reaction mechanism postulated in 1985 to account for the rapid photoisomerization of the retinyl chromophore in rhodopsin. The requisite stereochemical consequence of simultaneous isomerization of a double bond and an adjacent single bond has recently been demonstrated in isomerization of pre-vitamin D in an organic glass and by many other examples of organic systems already reported in the literature This paper reports the consequence in applying the mechanism to the primary photochemical process of several photosensitive biopigments: bilirubin, photoactive yellow protein, bacteriorhodopsin and rhodopsin. It is shown that the anchored nature of the chromophores must first be taken into consideration.

• 한국인쇄학회지
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• 제16권3호
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• pp.147-156
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• 1998

A Photosensitive Crown Ether Styryl Dye derivative(CESD) was prepared for the application and the structure of it was discussed. Light excitation causes the trans-cis isomerization of CESD yielding a conformation suitable to form a coordination bond between an anion group and a metal cation located in crown ether. Intermolecular complex stabilized the cis isomer that absorbs at a shorter wavelength in the trend-cis isomerization. Application of CESD was suggested.

• Bulletin of the Korean Chemical Society
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• 제29권11호
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• pp.2202-2204
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• 2008

• Bulletin of the Korean Chemical Society
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• 제30권5호
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• pp.1195-1197
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• 2009

• 전기화학회지
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• 제5권4호
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• pp.189-191
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• 2002

Reversible trans-cis isomerization of meta-bipyridylazobenzene coordinated to cobalt was achieved by a combination of photoirradiation with a single UV light source and a Co(II)/Co(III) redox reaction. The trans/cis conversion performance was significantly improved in the meta-form compared with the meta-form ligated to cobalt.

• Bulletin of the Korean Chemical Society
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• 제25권1호
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• pp.27-28
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• 2004

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 1999년도 학술발표회 진행표 및 논문초록
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• pp.38-38
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• 1999

Calculations on conformational free energies of Ac-Pro-NHMe and its $C^{\delta}$-methylated derivatives have been carried out with the higher levels of quantum-chemical methods to figure out the cis-trans isomerization of the imide bond of proline and $C^{\delta}$-methylated prolines in the gas phase and in solution.(omitted)hase and in solution.(omitted)

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2007년도 하계학술대회 논문집 Vol.8
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• pp.76-76
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• 2007

Several kinds of photo-chromic polymers containing push-pull structure were synthesized and investigated on optical patterning by photo-induced surface relief gratings (SRG) technique. The azobenzene segment was introduced as a functional group for a photo-triggered tran-cis isomerization. Consequently, we have fabricated micro-size regular pattern by one-step process without photo-mask.