• 제목/요약/키워드: isolation wall

검색결과 136건 처리시간 0.032초

Trichoderma koningii의 conidiospore로부터의 원형질체 분리에 관하여 (Isolation of protoplast from conidiospore of Trichoderma koningii)

  • 박희문;홍순우;하영칠
    • 미생물학회지
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    • 제21권4호
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    • pp.213-220
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    • 1983
  • Conditions for isolation of protoplasts from conidiospores of Trichoderma koningii ATCC 26113 were tested. Maximum production of conidial protoplasts was obtained by preincubation of conidiospores on liquid minimal medium for 8 1/2 hrs. and by reaction with cell wall lytic enzyme for 3 hrs. Among effective cell wall lytic enzymes (Driselase, p-Glucuronidase, Novozyme and Zymolyase), Driselase was the most effective one on the production of conidial protoplasts. The production of conidial protoplasts was also enhanced by addition of 2-Deoxy-D-Glucose $(25{\mu}g/ml)$ into liquid minimal medium. Over 70% of the initial swollen conidia, preincubated in liquid minimal medium supplemented with 2-Deoxy-D-Glucose $(25{\mu}g/ml)$, were converted to protoplasts by incubation with 2% (w/v) commercial lytic enzyme Driselase at $28^{\circ}C$ for 3 hrs. The reversion frequency of the conidial protoplasts was about 30 times (25-50%) higher than that of mycelial protoplasts (0.6-1.3%).

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고집적 회로를 위한 경사면 SWAMI 기술과 누설전류 분석 (The Technology of Sloped Wall SWAMI for VLSI and Analysis of Leakage Current)

  • 이용재
    • 한국통신학회논문지
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    • 제15권3호
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    • pp.252-259
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    • 1990
  • 本 論文은 기존 LOCOS工程의 張點을 모두 겸비한 側面璧 SWAMI 技術에 대한 새로운 構造를 提示한다. 새로운 SWAMI공정은 순수 窒化膜 壓力과 體積 膨腸에 기인한 壓力을 크게 줄이기 위해서 側面璧 주위에 얇은 질화막과 反應性이온 飾刻으로 기울어진 실리콘 측면벽을 結合시켰따. 製作된 結果에 의하면, 缺陷이 없는 완전히 새부리 모양이 形成되지 않는 局地的 酸化 공정은 기울어진 面의 異方性 산화 隔離에 의해 實現시킬 수 있었다. 추가적인 마스크 段階는 要求되지 않는다. 이 工程에서 PN 다이오드의 漏泄電流는 기존 LOCOS 공정 보다 減少되었다. 한편 가장자리 部位는 漏泄電流 密度에서 평편한 接合 부위 보다 높게 分析되었다.

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기초지반강성을 고려한 벽식구조 아파트의 지진응답에 관한 연구 (Study on Seismic Response of Wall-Slab Apartment Building Sturucture Considering the Stiffnesses of a Foundation-Soil System)

  • 김지원
    • 한국지진공학회:학술대회논문집
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    • 한국지진공학회 2000년도 추계 학술발표회 논문집 Proceedings of EESK Conference-Fall 2000
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    • pp.167-175
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    • 2000
  • Seismic analyses of structures can`t be performed without considering the effect of soil-structure interaction and seismic responses of a structure taking into account the stiffnesses of a foundation-soil system show a significant difference from those with a rigid base. However, current seismic analyses of apartment building structures were carried out assuming a rigid base and ignoring the characteristics of a foundation and the properties of the underlying soil. In this study, seismic analyses of apartment buildings of a particular wall-slab structural type were carried out comparing seismic response spectra of a flexible base with those of a rigid base and UBC-97. Wall-slab type low-rise or mid-height apartment buildings built on the deep soil layer showed a rigid body motion with the reduced seismic responses due to the base isolation effect, indicating that it is too safe but uneconomical to utilize the design spectra of UBC-97 for the seismic analysis of a wall-slab type apartment buildings due to the too conservative design.

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진동차단공의 재료별 방진효과에 관한 해석적 연구 (Analytical Study on the Isolation Effects of Bore Holes by Materials)

  • 이강일
    • 한국지반신소재학회논문집
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    • 제5권2호
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    • pp.23-30
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    • 2006
  • 본 연구는 환경진동으로 유발되는 진동 대책 중 전파 경로상에 방진구나 방진벽 등과 같은 진동차단벽을 설치했을 경우 차단벽의 재료를 방진구, 콘크리트, EPS, 철로 변화시켜 유한요소해석을 수행하여 진동을 가장 효율적으로 저감시킬 수 있는 재료 및 차단벽의 규모(타설심도, 차단벽두께, 진원으로부터의 거리)에 대하여 연구하였다. 본 연구 결과 방진구의 경우는 차단벽의 두께의 영향보다는 차단벽의 타설심도에 영향이 크고, 콘크리트의 경우에는 다른 방진벽의 방진효과에 비해 방진효과가 떨어진다. 또한 EPS의 경우는 타설심도 보다는 차단벽두께의 영향이 진동차단에 더 효과적임을 알 수 있었다.

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공동주택 리모델링 현장의 바닥충격음 저감대책 (Reduction Method for Floor Impact Noise on APT Remodeling)

  • 박철용;홍구표;이중원
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2009년도 춘계학술대회 논문집
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    • pp.524-525
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    • 2009
  • Heavy-weight impact noise(HN) is the most irritating noise in Korean apartment houses. It has been proclaimed standard floor system of 210mm thick slab with isolation material in the wall type structure. But this regulation is applied only new construction field and is not considered remodeling field. In general, the LN can be reduced by using isolation material but HN is known as relating with stiffness, strength & boundary condition of slab. Therefore it is difficulty in improving the HN on remodeling field. In this study, We conducted the reinforcement of concrete slab using C.F.S.(carbon fiber sheet), steel plate and steel beam after on-dol with isolation material on the remodeling field. As the test results, It appeared using C.F.S was no improved but using steel plate & steel beam were a little improved on HN.

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Shaking table experiment on a steel storage tank with multiple friction pendulum bearings

  • Zhang, Ruifu;Weng, Dagen;Ge, Qingzi
    • Structural Engineering and Mechanics
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    • 제52권5호
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    • pp.875-887
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    • 2014
  • The aim of the shaking table experiment is to verify the isolation effect of a storage liquid tank with multiple friction pendulum bearings. A 1:20 scale model of a real storage liquid tank that is widely used in the petroleum industry was examined by the shaking table test to compare its anchored base and isolated base. The seismic response of the tank was assessed by employing the time history input. The base acceleration, wave height and tank wall stress were used to evaluate the isolation effect. Finally, the influences of the bearing performance that characterizes the isolated tank, such as the friction force and residual displacement, were discussed.

Seismic Isolation Systems Incorporating with RC Core Walls and Precast Concrete Perimeter Frames -Shimizu Corporation Tokyo Headquarter-

  • Shimazaki, Dai;Nakagawa, Kentaro
    • 국제초고층학회논문집
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    • 제4권3호
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    • pp.181-189
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    • 2015
  • Shimizu Corporation Tokyo Headquarters, one of the city's leading office buildings, features many pioneering technologies that contribute to a sustainable society through environmental stewardship and a sophisticated disaster management facility. In terms of structural engineering, a seismic isolation system incorporating reinforced concrete core walls and precast concrete perimeter frames create a robust structure in the event of a large earthquake. In addition to the seismic resistance of the structure, several pioneering construction methods and materials are adopted. This office building can serve as a basis for new design and construction approaches and methodologies to ensure safe and economical structures.

Smart passive control of buildings with higher redundancy and robustness using base-isolation and inter-connection

  • Murase, Mitsuru;Tsuji, Masaaki;Takewaki, Izuru
    • Earthquakes and Structures
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    • 제4권6호
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    • pp.649-670
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    • 2013
  • It is known that a base-isolated building exhibits a large response to a long-duration, long-period wave and an inter-connected system without base-isolation shows a large response to a pulse-type wave. To compensate for each deficiency, a new hybrid passive control system is investigated in which a base-isolated building is connected to another building (free wall) with oil dampers. It is demonstrated that the present hybrid passive control system is effective both for pulse-type ground motions and long-duration and long-period ground motions and has high redundancy and robustness for a broad range of disturbances.

Response of base-isolated liquid storage tanks to near-fault motions

  • Jadhav, M.B.;Jangid, R.S.
    • Structural Engineering and Mechanics
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    • 제23권6호
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    • pp.615-634
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    • 2006
  • Seismic response of the liquid storage tanks isolated by the elastomeric bearings and sliding systems is investigated under near-fault earthquake motions. The fault normal and parallel components of near-fault motion are applied in two horizontal directions of the tank. The continuous liquid mass of the tank is modeled as lumped masses known as sloshing mass, impulsive mass and rigid mass. The corresponding stiffness associated with these lumped masses has been worked out depending upon the properties of the tank wall and liquid mass. It is observed that the resultant response of the isolated tank is mainly governed by fault normal component with minor contribution from the fault parallel component. Further, a parametric study is also carried out to study the effects of important system parameters on the effectiveness of seismic isolation for liquid storage tanks. The various important parameters considered are: aspect ratio of tank, the period of isolation and the damping of isolation bearings. There exists an optimum value of isolation damping for which the base shear in the tank attains the minimum value under near-fault motion. The increase of damping beyond the optimum value will reduce the bearing and sloshing displacements but increases the base shear. A comparative performance of five isolation systems for liquid storage tanks is also studied under normal component of near-fault motion and found that the EDF type isolation system may be a better choice for design of isolated tank in near-fault locations. Finally, it is also observed that the satisfactory response can be obtained by analysing the base-isolated tanks under simple cycloidal pulse instead of complete acceleration history.

.betha.-1, 3-glucanase 생성균의 분리 및 효소 생성 조건 (Isolation of .betha.-1, 3-glucanase producing strain and cultural conditions of its enzyme production)

  • 정기택;방광웅;송형익;김재근;유대식
    • 미생물학회지
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    • 제24권3호
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    • pp.295-301
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    • 1986
  • The bacteria, which were capable of producing ${\beta}-1$, 3-glucanase inducibly by utilizing cell wall of Aspergillus fumigatus as a sole carbon source, were isolated from soil in the campus of Kyungpook National University. Among them, the strain which produced the enzyme excellently was selected and identified to be Pseudomonas stutzeri KF 13 by morphological, cultural and physiological examination. The optimal conditions for the enzyme production from Pseudomonas stutzeri KF 13 were investigated. the enzyme production was reached maximum state shen the broth cultured for 72hr at $30^{\circ}C$. And the enzyme showed the highest activity in the medium containing 3.5% cell wall as an inducer, 15% yeast autolysate as a nitrogen source and 0.05% $MnSO_4$ at pH 7.5.

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