• 제목/요약/키워드: isolation techniques

검색결과 232건 처리시간 0.028초

MIMO RF 중계기를 위한 적응 신호처리 기반의 간섭 제거 (Interference Cancellation Based on Adaptive Signal Processing for MIMO RF Repeaters)

  • 이규범;최지훈
    • 한국통신학회논문지
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    • 제35권9C호
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    • pp.735-742
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    • 2010
  • 본 논문에서는 다수의 송신 안테나와 수신 안테나를 갖는 RF 중계기를 위한 적응 간섭제거 알고리즘을 제안한다. 중계기에서 다수의 안테나를 사용하는 경우 송신 안테나와 수신 안테나간의 불완전한 격리로 인해 간섭이 발생하고, 궤환 간섭 경로는 MIMO (multi-input multi-output) 채널로 모델링된다. 이런 궤환 간섭을 제거하기 위해 적응 신호처리 기법에 기반하여 LMS (least mean square) 간섭제거 알고리즘과 RLS (recursive least squares) 간섭제거 알고리즘을 유도한다. 모의 실험을 통해 제안된 간섭 제거 알고리즘의 수렴 특성 및 정상 상태에서의 간섭 제거 성능을 비교한다.

A Concurrency Control Scheme over T-tree in Main Memory Databases with Multiversion

  • Ying Xia;Cho, Sook-Kyoung;Oh, Young-Hwan;Kim, June;Bae, Hae-Young
    • 한국정보과학회:학술대회논문집
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    • 한국정보과학회 2000년도 가을 학술발표논문집 Vol.27 No.2 (1)
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    • pp.210-212
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    • 2000
  • In this paper, we present a comcurrency control scheme over the index structure in main memory databases using multiversion mechanism, and implement it on T-tree. As a well-known idea for comcurrency control, multiversion allows multiple transactions to reat and write different versions of the same data item, each transaction sees a consistent set of versions for all the data items it accesses[1]. Logical versioning and physical versioning techniques are used to keep versions of data item and versions of index node respectively. The main features of this multiversion indexing approach are (1)update operations and rotations on T-tree can take place concurrently, (2)the number of locking and latching requirement is sharply reduced because read-only transactions do not obtain any locks or latches and update transactions obtain latches only when actually performing the update, (3)it reduces storage overhead for tracking version and reclaims storage in time, and (4)it provides complete isolation of read-only transactions from update transactions, so the read-only transactions can get response information without any block.

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Cytotoxic Components in an Extract from the Leaves and Stems of Stauntonia hexaphylla

  • Zhao, Jing;Yim, Soon-Ho;Um, Jung-In;Park, Si-Hwan;Oh, Eun-Sang;Jung, Da-Woon;Williams, Darren R.;Lee, Ik-Soo
    • Natural Product Sciences
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    • 제20권2호
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    • pp.130-134
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    • 2014
  • An investigation was carried out to identify novel anti-cancer compounds from Korean indigenous plant extracts. Bioassay-guided fractionation and chemical investigation of the EtOAc extract from the leaves and stems of Stauntonia hexaphylla resulted in the isolation of two active compounds, hederagenin 3-O-${\alpha}$-L-arabinoside (1) and quercetin (2). The structures of these compounds were elucidated by spectroscopic methods, including UV, IR, MS, NMR techniques and compared with previous spectroscopic data. The cytotoxic effects of fractions and compounds on HCT116 human colon cancer cells were evaluated using the MTT assay. Quercetin showed a stronger anti-cancer effect when compared to hederagenin 3-O-${\alpha}$-L-arabinoside.

Anti-oxidative Activities of 3'-Hydroxygenkwanin from the Flower Buds of Daphne genkwa in Caenorhabditis elegans

  • Park, Sung-Hoon;Cui, Xun;Ahn, Dalrae;Lee, Eun Byeol;Cha, Dong Seok;Jeon, Hoon;Zee, Ok Pyo;Kim, Youn-Chul;Kim, Dae Keun
    • Natural Product Sciences
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    • 제20권2호
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    • pp.80-85
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    • 2014
  • In the course of screening for antioxidant compounds from natural plants in Korea by measuring the radical scavenging effect, a methanol extract of the flower buds of Daphne genkwa S. et Z. (Thymelaeaceae) was found to show a potent antioxidant activity. Subsequent activity-guided fractionation of methanol extract of D. genkwa led to the isolation of four compounds from the ethyl acetate soluble fraction. The chemical structures were elucidated as genkwanin (1), 3'-hydroxygenkwanin (2), apigenin (3), and tiliroside (4) by spectroscopic techniques. Among them, compound 2 showed the significant anti-oxidative effect on DPPH. And compound 2 showed the significant riboflavin-and xanthine-originated superoxide quenching activities. To verify the antioxidant enzymatic activities of compound 2, the SOD enzymatic activity was measured spectrophtometrically using prepared Caenorhabditis elegans homogenates. The results showed that compound 2 was able to elevate SOD activity of C. elegans in a dose dependent manner. Moreover, compound 2 decreased the intracellular ROS accumulation of worms.

Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus

  • Yoon, Seung-Jae;Seo, Kyoung-Won;Song, Kun-Ho
    • 대한수의학회지
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    • 제58권1호
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    • pp.27-31
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    • 2018
  • Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between $1.97{\times}10^4/mL$ and $9.85{\times}10^3/mL$ for samples with a 2-fold serial dilution from $1.25{\times}10^6\;TCID_{50}$ ($TCID_{50}$, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.

Meroparamycin Production by Newly Isolated Streptomyces sp. Strain MAR01: Taxonomy, Fermentation, Purification and Structural Elucidation

  • El-Naggar Moustafa Y.;El-Assar Samy A.;Abdul-Gawad Sahar M.
    • Journal of Microbiology
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    • 제44권4호
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    • pp.432-438
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    • 2006
  • Twelve actinomycete strains were isolated from Egyptian soil. The isolated actinomycete strains were then screened with regard to their potential to generate antibiotics. The most potent of the producer strains was selected and identified. The cultural and physiological characteristics of the strain identified. the strain as a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene (1.5kb) of the most potent strain evidenced a 99% similarity with Streptomyces spp. and S. aureofaciens 16S rRNA genes, and the isolated strain was ultimately identified as Streptomyces sp. MAR01. The extraction of the fermentation broth of this strain resulted in the isolation of one major compound, which was active in vitro against gram-positive, gram-negative representatives and Candida albicans. The chemical structure of this bioactive compound was elucidated based on the spectroscopic data obtained from the application of MS, IR, UV, $^1H$ NMR, $^{13}C$ NMR, and elemental analysis techniques. Via comparison to the reference data in the relevant literature and in the database search, this antibiotic, which had a molecular formula of $C_{19}H_{29}NO_2$ and a molecular weight of 303.44, was determined to differ from those produced by this genus as well as the available known antibiotics. Therefore, this antibiotic was designated Meroparamycin.

염기성 세균 배양을 위한 Steel Wool법과 Gaspak법의 비교 (Comparative Studies of Steel Wool Method and Gaspak Method for the Culture of Anaerobic Bacteria)

  • 정윤섭
    • 미생물학회지
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    • 제12권3호
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    • pp.131-137
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    • 1974
  • It is a well-known fact that an isolation of non-sporeforming anaerobes, considered normal flora in man ordinarily but causes serious infections sometimes, is a dificult procedure because of their great oxygen sensitivity. Among the many techniques employed in clinical laboratories, despite of its high expenses, the GasPak method has been most widely used because of its relative simplicity. On the other hand, the steel wool method has gained a good reputation recently. This technique makes it possible to treat individual plate so that any single specimen can be promptly cultured anaerobically. The procedure is quite simple and the expenses are negligible. In the present study it is to compare these two methods as to their efficiency of anaerobic cultivation using 13 VPI strains of non-sporeforming amaerobic bacteria. Among the 13 species the following 11, Bacteroides fragilis ss. fragilis, B. fragilis ss. thetaiotaomicron, propionibacterium acnes, Eubacterium limosum, E. lentum, peptococcus asaccharolyticus, Pc. prevotii, Pc. magnus, peptostreptococcus anaerobius, Ps. intermedius nad Veillonella parvula, grew well with the steel wool method whose colony numbers reaching 57 to 119% of those with GasPak method. The remaining two species, Fusobacterium nucleatum and F.necrophorum, did not grow well with the steel wool method showing the colony numbers were only 0.4% of those with GasPak method in the case of Fusobacterium nucleatum. In the case of Fusobacterium necrophorum, very few colonies developed even with a heavy inoculation. As to the size of colonies, there were no significant difference between these two methods.

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Bioprocess Strategies and Recovery Processes in Gibberellic Acid Fermentation

  • Shukla, Ruchi;Srivastava, Ashok K.;Chand, Subhash
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제8권5호
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    • pp.269-278
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    • 2003
  • Gibberellic acid (GA$_3$) is a commercially important plant growth hormone, which is gaining much more attention all over the world due to its effective use in agriculture and brewing industry. Industrially it is produced by submerged fermentation technique using Ascomycetous fungus Gibberella fujikuroi. Solid state and immobilized cell fermentation techniques had also been developed as an alternative to obtain higher yield of GA$_3$. This review summarizes the problems of GA$_3$ fermentation such as production of co-secondary metabolites along with GA$_3$, substrate inhibition and degradation of GA$_3$ to biologically inert compound gibberellenic acid, which limits the yield of GA$_3$ in the fermentation medium. These problems can be overcome by various bioprocessing strategies e.g. two - stage and fed batch cultivation processes. Further research on bioreactor operation strategies such as continuous and / or extractive fermentation with or without cell recycle / retention system need to be investigated for improvement in yield and productivity. Down stream processing for GA$_3$ isolation is also a challenge and procedures available for the same have been critically evaluated.

An Investigation of the deformation of underground excavations in slat and potash mines

  • 권상기
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 1998년도 터널.암반역학위원회 박사학위 논문집
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    • pp.83-114
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    • 1998
  • The most widely accepted method for understanding the deformation mechanism of rock is from the use of computer simulation. However, if the changes in rock properties after excavation are significant this will prevent the computer simulation kent predicting the deformation with acceptable accuracy. If the deformations are, however, carefully measured in situ, the resulting data can be more useful far predicting the deformational behavior of underground openings, since the effect of the parameters which influence the deformational behavior are included in the measurement. In this study, extensive data analyses were carried out using the deformation measurements from the Waste Isolation Pilot Plant (WIPP), which is a permanent nuclear waste repository The results from computer simulations were compared with field measurements to evaluate the assumptions used in the computer simulations, For better description of the deformational behavior around underground excavations, several techniques were developed, namely: (a) the calculation of the zero strain boundary; (b) the evaluation of the influence of adjacent excavations on the deformational behavior of pre-excavated openings; (c) the description of the deformational behavior using in situ measurements; (d) the calculation of the shear stress distribution; and (e) the application of a Neural Network for the prediction of opening deformation.

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Isolation and Characterization of a New Alkaloid from the Seed of Prunus persica L. and Its Anti-inflammatory Activity

  • Rho, Jung-Rae;Jun, Chang-Soo;Ha, Young-Ae;Yoo, Myung-Ja;Cui, Ming-Xun;Baek, Hwa-Seung;Lim, Jin-A;Lee, Young-Haeng;Chai, Kyu-Yun
    • Bulletin of the Korean Chemical Society
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    • 제28권8호
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    • pp.1289-1293
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    • 2007
  • Persicaside has been isolated as a new alkaloid natural compound from a methanol (EtOA)-soluble extract of Prunus persica seed. It was purified by a combination of chromatographic techniques and recrystallization. The structure of Persicaside was determined by extensive NMR experiments and mass ppectroscopic data. It inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) production via suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2 expression in rat osteoblast sarcoma cells (ROS 17/2.8) in concentration-dependent manner whereas it spares the COX-1 enzyme activity.