• 제목/요약/키워드: isolation and structure determination

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Isolation and Structure Determination of a Proteasome Inhibitory Metabolite from a Culture of Scytonema hofmanni

  • Shim, Sang-Hee;Chlipala, George;Orjala, Jimmy
    • Journal of Microbiology and Biotechnology
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    • 제18권10호
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    • pp.1655-1658
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    • 2008

  • Cyanobacteria, blue-green algae, are a rich source of bioactive secondary metabolites with many potential applications. The ubiquitin-proteasome proteolytic system plays an important role in selective protein degradation and regulates cellular events including apoptosis. Cancer cells are more sensitive to the proapoptotic effects of proteasome inhibition than normal cells. Thus, proteasome inhibitors can be potential anticancer agents. Cyanobacteria have been shown to be a rich source of highly effective inhibitors of proteases. A proteasome inhibitor was screened from an extract of the culture of Scytonema hofmanni on the basis of its inhibitory activity, which led to the isolation of nostodione A with an $IC_{50}$ value of 50${\mu}M$. Its structure was determined by spectroscopic methods such as $^{1}H$-NMR and ESI-MS spectral analyses.

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Streptomyces melanosporofaciens가 생산하는 새로운 항생물질 II. 물질의 항균활성과 황성물질의 분리.정제 및 구조결종 (New Antibiotics Produced by Streptomyces melanosporofaciens II. Antimicrobial Activities and Isolation, Purification, and Structure Determination of the Active Compound)

  • 김시관;김상석;김근수;정영륜;김창한
    • 한국미생물·생명공학회지
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    • 제19권3호
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    • pp.235-241
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    • 1991

  • Streptomyces melanosporofaciens로 동정된 strain 88-GT-161 균주는, 그람양성세균 및 식물병원성 곰팡이에 각각 항균활성을 나타내는 phthalate 유도체 및 염기성 마크로라이드의 새로운 항생물질을 생산하는 것으로 밝혀졌다. 이들 활성물질들의 분리. 정제과정에서 in vitro 및 in vivo(포트 시험) 항균활성을 조사하였으며, phtahalate 유도체 항생물질은 IR, NMR, 질량분석 스펙트럼 조사를 통하여 bis(2-ethylexyl) phthalate(dioctyl phthalate)로 동정하였다. Dioctyl phthalate가 Streptomyces sp.에 의하여 생산되며 생합성된 이 화합물이 항균활성을 가진다는 사실이 보고된 것은 이것이 처음이다.

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Investigation on seismic isolation retrofit of a historical masonry structure

  • Artar, Musa;Coban, Keziban;Yurdakul, Muhammet;Can, Omer;Yilmaz, Fatih;Yildiz, Mehmet B.
    • Earthquakes and Structures
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    • 제16권4호
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    • pp.501-512
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    • 2019

  • In this study, seismic vulnerability assessment and seismic isolation retrofit of Bayburt Yakutiye Mosque is investigated. Bayburt Yakutiye Mosque was built in the early 19th century at about 30-meter distance to Coruh river in the center of Bayburt in Turkey. The walls of historical masonry structure were built with regional white and yellow stones and the domes of the mosque was built with masonry bricks. This study is completed in four basic phases. In first phase, experimental determination of the regional white stone used in the historical structure are investigated to determine mechanical properties as modulus of elasticity, poison ratio and compression strengths etc. The required information of the other materials such as masonry brick and the regional yellow stone are obtained from literature studies. In the second phase, three dimensional finite element model (FEM) of the historical masonry structure is prepared with 4738 shell elements and 24789 solid elements in SAP2000 software. In third phase, the vulnerability assessment of the historical mosque is researched under seismic loading such as Erzincan (13 March 1992), Kocaeli (17 August 1999) and Van (23 November 2011) earthquakes. In this phase, the locations where damage can occur are determined. In the final phase, rubber base isolators for seismic isolation retrofit is used in the macro model of historical masonry mosque to prevent the damage risk. The results of all analyses are comparatively evaluated in details and presented in tables and graphs. The results show that the application of rubber base isolators can prevent to occur the destructive effect of earthquakes.

  • https://doi.org/10.12989/eas.2019.16.4.501 인용 KSCI