• Korean Journal of Veterinary Research
• /
• v.34 no.3
• /
• pp.517-527
• /
• 1994

An attempt was made to isolate a causative viral agents from the fecal specimens of the diseased dogs with the gastroenteritis symptoms. Two coronavirus-like agents were isolated by serial dilution end point method and plaque assay. The isolates were characterized in terms of cytopathology, antigenicity, replication, physicochemical and morphological properties. The results obtained through the experiment were as follows; 1. Among 7 fecal specimens collected from the dogs with enteric disease, 2(28.6%)coronavirus-like agents showing typical cytopathic effects of canine coronavirus were isolated, and designated as CCV D1 and CCV D2, respectively. 2. By the cross-neutralization test and indirect immunofluoresence antibody test, the isolates were antigenically indentified as the standard CCV. The viruses were replicated only in the cytoplasm of A-72 cells. 3. The isolates showed no haemagglutinating activity against the erythrocytes from 11 kinds of animals. 4. The electron microscopic observation for the isolates showed spherical and pleomorphic features, covered with club-shaped projections on the surface. The size of particles was ranged from 70 to 150nm. 5. In one-step growth curve for the isolates in A-72 cells, maximum titers of intracellular vius was $10^{4.6}$ $TCID_{50}/0.1ml$ at 46 hrs postinoculation(pi) of CCV Dl and $10^{4.4}$ $TCID_{50}/0.1ml$ at 34 hrs pi of CCV D2. The maximum titers of extracellular virus was $10^{5.5}$ $TCID_{50}/0.1ml$ at 58 hrs pi of CCV D1 and $10^{5.8}$ $TCID_{50}/0.1ml$ at 46 hrs pi of CCV D2. 6. In physicochemical property test, the isolates were very sensitive to choroform and were found to be RNA virus. The viruses was stable at pH 3.0 for 1 hr and at $22{^{\circ}C}$ for 5 hrs. However, infectivity titers reduced remarkably by treatment with $56{^{\circ}C}$ for 10min.

• Journal of the Korean Ceramic Society
• /
• v.30 no.6
• /
• pp.449-456
• /
• 1993

Sintering behaviour of zirconia powders prepared by different processing treatment was discussed. About >99% densities of theoretical were obtaiend on sintering at 140$0^{\circ}C$ for 2h in case of 300MPa uniaxially cold-pressed compact. But the lower densities were obtained on sintering above this temperature due to abnormal grain growth enabling the tetragonal to monoclinic phase transformation during cooling resulted in microcracks. All kinds of different dried powders exhibited nearly the same shrinkage behaviour with end-point shrinkage between 19 and 20%, and had maximum shrinkage rate (0.99~1.27%/min) around 120$0^{\circ}C$. During whole sintering process densification was mainly governed by grain growth and rearrangement of agglomerates. Heterogeneous abnormal grain growth and abrupt decrease in shrinkage were observed when continuous interagglomerate pore collapsed into isolated pores.

• Journal of Power Electronics
• /
• v.9 no.2
• /
• pp.320-333
• /
• 2009

A Photovoltaic (PV) system's power output varies with the change of climate. Frequency deviations, tie line voltage swings are caused by the varying PV power when large PV power from several PV systems is fed in the utility. In this paper, to overcome these problems, a simple coordinated control method for smoothing the variations of combined PV power from multiple PV systems is proposed. Here, output power command is formed in two steps: central and local. Fuzzy control is used to produce the central smoothing output power command considering insolation, variance of insolation and absolute average of frequency deviation. In local step, a simple coordination is kept between the central power command and the local power commands by producing a common tuning factor. Power converters are used to achieve the same output power as local command power employing PI control law for each of the PV generation systems. The proposed method is compared with the method where conventional Maximum Power Point Tracking (MPPT) control is used for each of the PV systems. Simulation results show that the proposed method is effective for smoothing the output power variations and feasible to reduce the frequency deviations of the power utility.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.11
• /
• pp.1597-1602
• /
• 2010

Chitinase is one of the most important mycolytic enzymes with industrial significance. This enzyme is produced by a number of organisms including bacteria. In this study, we describe the optimization of media components with increased production of chitinase for the selected bacteria, Stenotrophomonas maltophilia, isolated from soil. Different components of the defined media responsible for influencing chitinase secretion by the bacterial isolate were screened using Plackett-Burman experimental design and were further optimized by Box-Behnken factorial design of response surface methodology in liquid culture. Maximum chitinase production was predicted in medium containing 4.94 g/l chitin, 5.56 g/l maltose, 0.62 g/l yeast extract, 1.33 g/l $KH_2PO_4$, and 0.65 g/l $MgSO_4{\cdot}7H_2O$ using response surface plots and the point prediction tool of the DESIGN EXPERT 7.1.6 (Stat-Ease, USA) software.

• Journal of Electrical Engineering and Technology
• /
• v.9 no.5
• /
• pp.1643-1653
• /
• 2014

This paper presents the design and implementation of a wind-solar hybrid power system for LED street lighting and an isolated power system. The proposed system consists of photovoltaic modules, a wind generator, a storage system (battery), LED lighting, and the controller, which can manage the power and system operation. This controller has the functions of maximum power point tracking (MPPT) for the wind and solar power, effective charging/discharging for the storage system, LED dimming control for saving energy, and remote data logging for monitoring the performance and maintenance. The proposed system was analyzed in regard to the operation status of the hybrid input power and the battery voltage using a PSIM simulation. In addition, the characteristics of the proposed system's output were analyzed through experimental verification. A prototype was also developed which uses 300[W] of wind power, 200[W] of solar power, 60[W] LED lighting, and a 24[V]/80[Ah] battery. The control system principles and design scheme of the hardware and software are presented.

• Applied Biological Chemistry
• /
• v.41 no.3
• /
• pp.213-218
• /
• 1998

A bacterium with potent activity of pigment production and protease was isolated and identified as being Bacillus sp. CS-17. Cell growth, protease activity and pigment production of the strain reached to its maximum point after 24 hrs, 48 hrs, 72 hrs, respectively. The best pigment producing ability of Bacillus sp. CS-17 was shown on basal medium for pigment production added 1.0% soybean. The high effcient conditions for pigment production was obtained at culture of pH 8.5, $37^{\circ}C$ and 72 hours. Among the tested 5 gram positive strains and 6 gram negative strains, weak antibacterial activity of pigment concentrated extracts was appeared against growth of B. subtilis, P. aeruginosa, S. typhimurium, E. aerogenes, B. cereus, A. hydrophila.

• Journal of the Korean Institute of Telematics and Electronics C
• /
• v.36C no.6
• /
• pp.44-51
• /
• 1999

The HMM-Net is a neural network architecture that implements the computation of output probabilities of a hidden Markov model (HMM). The architecture is developed for the purpose of combining the discriminant power of neural networks with the time-domain modeling capability of HMMs. Criteria of maximum likehood(ML) and minimization of mean squared error(MMSE) are used for learning HMM-Net classifiers. The criterion MMSE is better than ML when initial learning condition is well established. However Ml is more useful one when the condition is incomplete[3]. Therefore we propose an efficient learning method of HMM-Net classifiers using a hybrid criterion(ML/MMSE). In the method, we begin a learning with ML in order to get a stable start-point. After then, we continue the learning with MMSE to search an optimal or near-optimal solution. Experimental results for the isolated numeric digits from /0/ to /9/, a training and testing time-series pattern set, show that the performance of the proposed method is better than the others in the respects of learning and recognition rates.

• Microbiology and Biotechnology Letters
• /
• v.20 no.2
• /
• pp.156-163
• /
• 1992

Bacillus sp. was isolated from soil for its strong activity of cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19). The enzyme was purified by gel filtration and anion exchange column chromatography using FPLC. The purified enzyme exhibited its maximum CGTase activity in the pH range of 6~8 and the temperature range of 50~$70^{\circ}C$. The molecular weight was estimated as 114,000 by SDS-PAGE. The isoelectric point of the enzyme was 4.3. The CGTase of Bacillus sp. E l produced $\beta$-cyclodextrin mainly and did not produce a-cyclodextrin. The product ratio of $\beta$-cyclodextrin to $\gamma$-cyclodextrin was 7:l.

• Applied Chemistry for Engineering
• /
• v.10 no.3
• /
• pp.394-399
• /
• 1999

As the prosthetic application of natural mineral substituted for chemical reagent, composites and a glass-ceramics containing hydro-xyapatite isolated from tuna bone were prepared by solid state reaction. On x-ray examinations, the major phases of composites were identified as pseudowollastonite(${\alpha}-CaSiO_3$) and ${\beta}$-tricalcium phosphate($\beta$-TCP) and the phase of a glass-ceramics was observed as $\beta$-TCP and fluoroapatite caused by $CaF_2$ respectively. SEM images depict that the microstructures of grain at the composites were a function of temperature. The measured strength of a glass-ceramics prepared at $900^{\circ}C$ for 4 hr in air was 90 MPa as a 4-point bending method and this value was similar to the cortical bone, as 50~150 MPa but it was lower than its maximum strength.

• The Korean Journal of Malacology
• /
• v.32 no.4
• /
• pp.241-247
• /
• 2016

Macrophage Migration Inhibitory Factor (MIF) are well-defined role as unique cytokine and critical mediator in acute and chronic inflammatory diseases, autoimmune diseases. In this study, we isolated and characterized a full-length of MIF cDNA from the abalone (Haliotis discus hannai). The full-length cDNA of abMIF was of 1264 bp, consisting of a 5'-terminal UTR of 143 bp, an open reading frame of 360 bp and a 3-terminal UTR of 761 bp. The abalone MIF cDNA encodes a 119-amino acid polypeptide with a calculated molecular mass of 13.4 kDa and isoelectric point of 9.07. Multiple alignments and phylogenetic analysis with the deduced abalone MIF protein and showed strong homology with disk abalone (Haliotis discusdiscus). The deduced amino acid sequence of abMIF exhibited homology with other reported MIFs, such as 80%, with that of other disk abalone H. discus discus MIF gene. Quantitative real-time PCR (qRT-PCR) analysis indicated that abMIF was highly expression observed in hapatopacreas, intestine, foot, and gonad of normal conditioned abalone. Even though AbMIF mRNA level in hemocytes was low under the normal condition, it was sharply up-regulated and reached the maximum at 6 h post-infection with Vibrio parahaemolyticus, and then decreased at 24 h post-infection. This result indicates that abMIF plays an important role in responding in the innate immune system.