• Journal of Applied Biological Chemistry
• /
• v.53 no.1
• /
• pp.60-64
• /
• 2010

None of genes from Lespedeza bicolor involved in isoflavonoid biosynthesis have been biochemically characterized. An isoflavone synthase from Lespedeza bicolor was cloned. To verify its catalytic activity, a fusion protein of LbIFS with P450 reductase from rice was made. Using this construct, production of isoflavone from flavanone was confirmed.

• Proceedings of the PSK Conference
• /
• 2002.10a
• /
• pp.354.3-355
• /
• 2002

Interleukin (IL)-5 appears to be one of the main proinflammatory mediators among a growing number of cytokines and chemokines that induce eosinophilic inflammation. Sophoricoside and their analogs isolated from Sophora japonica show relatively potent inhibitory activity of interleukin (IL)-5 as a small molecule. To identify structural requirements of this isoflavonone for its inhibitory activity against IL -5. isoflavonones. isoflavanones, and their glycosides were prepared and tested their inhibitory activity against IL-5. (omitted)

• Proceedings of the PSK Conference
• /
• 2003.04a
• /
• pp.233.1-233.1
• /
• 2003

Interleukin (IL)-5 appears to be one of the main proinflammatory mediators among a growing number of cytokines and chemokines that induce eosinophilic inflammation. Sophoricoside and their analogs isolated from Sophora japonica show relatively potent inhibitory activity of interleukin (IL)-5 as a small molecule. Initial attempt to identify the structural requirement of this isoflavonone led to find new chalcones to exhibit the inhibitory activity of IL -5. (omitted)

• Journal of Microbiology and Biotechnology
• /
• v.19 no.12
• /
• pp.1612-1616
• /
• 2009

Isoflavonoids are a class of phytoestrogens. Isoflavonone synthase (IFS) is responsible for the conversion of naringenin to genistein. IFS is a cytochrome P450 (CYP), and requires cytochrome P450 reductase (CPR) for its activity. Additionally, the majority of cytochrome P450s harbor a membrane binding domain, making them difficult to express in Escherichia coli. In order to resolve these issues, we constructed an inframe fusion of the IFS from red clover (RCIFS) and CPR from rice (RCPR) after removing the membrane binding domain from RCIFS and RCPR. The resultant fusion gene, RCIFS-RCPR, was expressed in E. coli. The conversion of naringenin into genistein was confirmed using this E. coli transformant. Following the optimization of the medium and cell density for biotransformation, $60\;{\mu}M$ of genistein could be generated from $80\;{\mu}M$ of naringenin. This fusion protein approach may be applicable to the expression of other P450s in E. coli.