• Title/Summary/Keyword: invertebrates

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Fabrication of Planar-type Semiconductor Microelectrode Array and Its Application to Peripheral Nerve of Invertebrates (평판형 반도체 미세전극 어레이 제작 및 이를 이용한 무척추 동물의 말초신경다발 신호기록)

  • Hwang, E.J.;Yoon, T.H.;Kim, S.J.
    • Proceedings of the KOSOMBE Conference
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    • v.1998 no.11
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    • pp.145-146
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    • 1998
  • A planar-type silicon microelectrode array has been fabricated and used successfully in obtaining simultaneous multichannel recordings from peripheral nerve of invertebrates. This paper demonstrates that planar-type semiconductor electrode arrays are useful for studying traveling wave properties of action potential.

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Recent Discovery of Bioactive Natural Products from Taiwanese Marine Invertebrates

  • Shen, Ya-Ching
    • Journal of Marine Bioscience and Biotechnology
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    • v.1 no.4
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    • pp.225-231
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    • 2006
  • The secondary metabolites from Taiwanese marine soft corals and sponges have attracted much attention because they possess considerable potential biological activities. To explore the origin of bioactivity, many cytotoxic natural products were isolated and characterized in the past few years. For examples, The lipophilic extracts from marine sponges Petrosia elastica and Ircinia formosana were found active against several human tumor cells. The investigation of the gorgonian Junceela has also resulted in the discovery of a series of new juncenolides. Bioassay-directed fractionation of Clavularia viridis yielded seven new prostanoids. These compounds have been tested and evaluated as potential antitumor agents. The soft corals of the genus Cespitularia produced novel secondary metabolites with diverse chemical structures and interesting biological activities. Four new norditerpenoids, designated cespitulactones and cespihypotins were isolated from Cespitularia hypotentaculata. Cespitulactones are novel structures having a bond cleavage between C-10 and C-11. In addition, three novel diterpenes were isolated from C. taeniata and designated cespitulactams A, B and C having a phenylethyl amino side chain.

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Fundamental Morphological Study of 16 Genera of Chironomid Larvae in Korea

  • Lee, Dong Ju;Park, Jae-won;Kwak, Ihn-Sil
    • Korean Journal of Ecology and Environment
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    • v.54 no.2
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    • pp.125-129
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    • 2021
  • Chironomids are a large group of invertebrates that live in various aquatic habitats. The distribution range of these invertebrates has become varied due to anthropogenic impacts; as such, their distribution can be used as an indicator of environmental health. Adult chironomids are well known in South Korea; however, the larvae have rarely been studied due to difficulties associated with morphological classification. To address this lack of information, we collected larvae from four important rivers in South Korea and summarized their taxonomic morphological characteristics. The antennae, mandible, and mentum were used for larval taxonomic characterization. In this study, we describe the basic morphological features and key pictorial features of 20 species of chironomids, representing 16 genera.

Activated Phenoloxidase Interacts with A Novel Glycine-rich Protein on the Yeast Two-hybrid System

  • Lee, Sun-Woo;Lee, Hyun-Seong;Kim, Eun-Jun;Yoo, Mi-Ae;Lee, Bok-Luel
    • BMB Reports
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    • v.34 no.1
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    • pp.15-20
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    • 2001
  • One of the innate immune reactions in invertebrates is the pro-phenoloxidase (pro-PO) activation system that is involved in the generation of superoxide, melanin synthesis, and the subsequent sequestration of foreign matter entering the hemocoel of the invertebrates. However, the molecular mechanism of this biological reaction is still obscure. To expand our understanding of the biological roles of the pro-PO activation system in invertebrates, we performed a yeast two-hybrid screening by using three regions of pro-PO as bait and a yeast two-hybrid cDNA library from Tenebrio molitor larvae as prey We isolated a novel partial cDNA clone that encodes a glycine-rich protein that interacted with the active phenoloxidase (termed phenoloxidase interacting protein, POIP). POIP consists of two domains: One is an N-terminal unique domain and the other is a C-terminal glycine-rich domain. The C-terminal glycine-rich domain showed sequential homology with those of insect antifungal proteins. Also, the yeast two-hybrid screen in a reverse orientation (using POIP as bait) yielded PO, suggesting that the PO-POIP interaction is specific. By using a 315 bP PCR fragment of the N-terminal unique region of POIP, we cloned the full-length cDNA of POIP from the Tenebruo cDNA library constructed by using E. coli injected larvae. The interaction analysis between PO, and a truncated fragment lacking the N-terminal unique region of POIP, indicated that the N-terminal unique region is necessary for interaction between PO and POIP. The expression level of the POIP mRNA is increased by bacterial injection into T. molitor larvae. This suggests that POIP might be engaged in the humoral defense reaction.

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Biodiversity of Benthic Macroinvertebrate on Organic Rice Paddy Field (유기농업 논에서 저서성대형무척추동물의 다양성)

  • Kim, Jong-Sun;Kim, Do-Ik;Kim, Seon-Gon;Kang, Beom-Ryong;Ko, Suk-Ju;Lim, Gyeong-Ho;Kim, Hong-Jae
    • Korean Journal of Organic Agriculture
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    • v.17 no.2
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    • pp.193-209
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    • 2009
  • Total collected benthic macro invertebrates in rice paddy fields were identified 3 phylum, 5 class, 13 order, 25 family, 32 genera and 36 species, those in organic rice field were 12 order, 22 family and 28 species, and in conventional rice field were 10 order, 19 family and 25 species. The almost collected species inhabit lentic zone but Semisulcospira libertina, Corbicula fluminea, and Tipula sp. were habitat on lotic zone. The number of benthic macro invertebrates species was higher in organic paddy field than in conventional field. Eleven species were collected only on organic but eight species only on conventional field. The number of individuals were 870 on organic rice field which higher 3.6-fold on conventional field. The diversity indices of organic rice field were 1.57-2.45 and 0.71-2.61 in the first and second survey, respectively. The higher diversity indices, the lower dominance indices.

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