• Title/Summary/Keyword: intra-band

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Identification of Different Species and Dultivars of Brassica by SDS-PAGE, Isozyme and Molecular Marker

  • Mukhlesur Rahman Md.;Hirata Yutaka
    • Journal of Plant Biotechnology
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    • v.7 no.1
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    • pp.27-35
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    • 2005
  • Eighty-five different cultivars of Brassica rapa, B. juncea, B. nap us, B. carinata, B. oleracea and hexaploid Brassica collected from Bangladesh, Japan, China and Denmark were analyzed by SDS-PAGE for seed and leaf protein variations, using esterase, acid phosphatase and peroxidase isozyme analysis. Ten polymorphic bands were identified from seed protein however no identifiable polymorphic band was found in the leaf protein. Polymorphic markers clearly distinguished the different Brassica species as well as yellow sarson (YS) and brown seeded (BS) cultivars of B. rapa. The $F_1$ cross between YS and brown seeded cultivars showed the existance of all poly-morphic bands of the respective parents. The Bangla-deshi and Japanese cultivars of B. rapa differed in the amount of seed protein. In the case of isozyme analysis, esterase showed the highest number of polymorphic bands (13) followed by acid phosphatase (9) and peroxidase (5). These polymorphic markers were very effec-tive for classification of all the species studied in this experiment. In parentage tests using isozymes, the hybridity of intra-and-interspecific crosses of almost all the seedlings could be identified from their respective cross combinations. Esterase polymorphism showed a clear differentiation between YS and BS types of B. rapa. In addition, two esterase polymorphic markers were iden ified to differentiate some cultivars of B. juncea. Segregation patterns in these two esterase bands showed a simple Mendelian monohybrid ratio of 3:1 in $F_2$, 1:1 in test cross and 1:0 in back cross progenies. No polymorphic band was identified to distinguish different cultivars of the same species by acid phosphatase or peroxidase. Polymerase Chain Reaction (PCR) was carried out with seed coat color specific marker of B. juncea. The yellow seeded cultivars produced a strong band at 0.5 kb and weak band 1.2 kb. In the addition of these two specific bands, Japanese yellow-seeded cultivars expressed two more weak bands at 1.0 kb and 1.1 kb. Where the brown seeded cultivars generated a single strong band at 1.1 kb. In segregating population, the yellow seed coat color marker segregated at a ratio 15 (brown) : 1 (yellow), indicating the digenic inheritance pattern of the trait.

The Meaning of P50 Suppression : Interaction of Gamma and Alpha Waves

  • Lee, Kyungjun;Kang, Ung Gu
    • Korean Journal of Biological Psychiatry
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    • v.21 no.4
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    • pp.168-174
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    • 2014
  • Objectives Sensory gating dysfunctions in patients with schizophrenia and bipolar disorder have been investigated through two similar methods ; P50 suppression and prepulse inhibition paradigms. However, recent studies have demonstrated that the two measures are not correlated but rather constitute as distinct neural processes. Recent studies adopting spectral frequency analysis suggest that P50 suppression reflects the interaction between gamma and other frequency bands. The aim of the present study is to investigate which frequency component shows more significant interaction with gamma band. Methods A total of 108 mood disorder patients and 36 normal subjects were included in the study. The P50 responses to conditioning and test stimuli with an intra-pair interval of 500 msec were measured in the study population. According to P50 ratio (amplitude to the test stimulus/amplitude to the conditioning stimulus), the subjects with P50 ratio less than 0.2 were defined as suppressed group (SG) ; non-suppressed group (NSG) consisted of P50 ratio more than 0.8. Thirty-five and 25 subjects were included in SG and NSG, respectively. Point-to-point correlation coefficients (PPCCs) of both groups were calculated between two time-windows : the first window (S1) was defined as the time-window of one hundred millisecond after the conditioning auditory stimulus and the second window (S2) was defined as the time-window of 100 msec after the test auditory stimulus. Spectral frequency analysis was performed to investigate which frequency band results in the difference of PPCC between SG and NSG. Results Significant reduction of PPCC between S1 and S2 was observed in the SG (Pearson's r = 0.24), compared to PPCC of the NSG (r = 0.58, p < 0.05). In spectral frequency analysis, gamma band showed "phase-reset" and similar responses after the two auditory stimuli in suppressed and non-suppressed group. However in the case of alpha band, comparison showed significantly low PPCC in SG (r = -0.14) compared to NSG (r = 0.36, p < 0.05). This may be reflecting "phase-out" of alpha band against gamma band at approximately 50 msecs after the test stimulus in the SG. Conclusions Our study suggests that normal P50 suppression is caused by phase-out of alpha band against gamma band after the second auditory stimulus. Thus it is demonstrated that normal sensory gating process is constituted with attenuated alpha power, superimposed on consistent gamma response. Implications of preserved gamma and decreased alpha band in sensory gating function are discussed.

Isolation and Characterization of Mitochondrial DNA from Arehicityars and Metacitrus (Archicitrus와 Metacitrus로부터 Mitochondrial DNA의 분리 및 특성)

  • 이숙영;박민희
    • The Korean Journal of Food And Nutrition
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    • v.8 no.4
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    • pp.307-317
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    • 1995
  • The purity of mtDNAs isolated from Archicitrus and Metacitrus leaves was higher in percoll density gradient centrifugation than differential and sucrose density gradient centrifugation. The most clear mtDNAs were obtained from mitochondria included in the Interface band of between 21% and 45% under isomotic, low viscosity conditions in the three step discontinuous percoll density gradient centrifugation. DNase treatment to the crude mitochondrlal suspension still more increased purity of mtDNA by the effective removal of the nuclear and chloroplast DNA and mtDNAs were appeared as a single band at middle position of tube by EtBr /cscl density gradient centrifugation. Agarose gel electrophoresis of mtDNAs resolved a single, broad band containing high molecular weight DNAs In all preparation. Yield of mtDNAs was about 110 and 2 ug Per 2009 in mature and immature leaves respectively. The mtDNA fragment patterns showed by EcoR I treatment were indistinguishable with respect to nom bet and position of bands in Archicitrus and Metacitrus. In the pattern of Hind E restriction, the Metacitrus displayed the unique band between 5.0 and 4.0kb, in addition to four fragments about 5.0, 2.4, 2.15, and 2.0kb, respectively, different from Archicitrus. Also the pattern of total mtDNAs fragment by the treatment of Pst I showed that the distinguishable fragment pat tern was not appeared in Archicitrus(C. iyo Tanaka), but about 6.0, 5.5, 5.0 and 2.Bkb fragments were appeared only in Metacitrus(C. junos Sieb). Therefore it was indicated that two species in intra-subgenus were identical each other, whereas considerable difference was revealed for inter-subgenus.

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Enhanced Spectral Hole Substitution for Improving Speech Quality in Low Bit-Rate Audio Coding

  • Lee, Chang-Heon;Kang, Hong-Goo
    • The Journal of the Acoustical Society of Korea
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    • v.29 no.3E
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    • pp.131-139
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    • 2010
  • This paper proposes a novel spectral hole substitution technique for low bit-rate audio coding. The spectral holes frequently occurring in relatively weak energy bands due to zero bit quantization result in severe quality degradation, especially for harmonic signals such as speech vowels. The enhanced aacPlus (EAAC) audio codec artificially adjusts the minimum signal-to-mask ratio (SMR) to reduce the number of spectral holes, but it still produces noisy sound. The proposed method selectively predicts the spectral shapes of hole bands using either intra-band correlation, i.e. harmonically related coefficients nearby or inter-band correlation, i.e. previous frames. For the bands that have low prediction gain, only the energy term is quantized and spectral shapes are replaced by pseudo random values in the decoding stage. To minimize perceptual distortion caused by spectral mismatching, the criterion of the just noticeable level difference (JNLD) and spectral similarity between original and predicted shapes are adopted for quantizing the energy term. Simulation results show that the proposed method implemented into the EAAC baseline coder significantly improves speech quality at low bit-rates while keeping equivalent quality for mixed and music contents.

Parallel Processing of k-Means Clustering Algorithm for Unsupervised Classification of Large Satellite Images: A Hybrid Method Using Multicores and a PC-Cluster (대용량 위성영상의 무감독 분류를 위한 k-Means Clustering 알고리즘의 병렬처리: 다중코어와 PC-Cluster를 이용한 Hybrid 방식)

  • Han, Soohee;Song, Jeong Heon
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.37 no.6
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    • pp.445-452
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    • 2019
  • In this study, parallel processing codes of k-means clustering algorithm were developed and implemented in a PC-cluster for unsupervised classification of large satellite images. We implemented intra-node code using multicores of CPU (Central Processing Unit) based on OpenMP (Open Multi-Processing), inter-nodes code using a PC-cluster based on message passing interface, and hybrid code using both. The PC-cluster consists of one master node and eight slave nodes, and each node is equipped with eight multicores. Two operating systems, Microsoft Windows and Canonical Ubuntu, were installed in the PC-cluster in turn and tested to compare parallel processing performance. Two multispectral satellite images were tested, which are a medium-capacity LANDSAT 8 OLI (Operational Land Imager) image and a high-capacity Sentinel 2A image. To evaluate the performance of parallel processing, speedup and efficiency were measured. Overall, the speedup was over N / 2 and the efficiency was over 0.5. From the comparison of the two operating systems, the Ubuntu system showed two to three times faster performance. To confirm that the results of the sequential and parallel processing coincide with the other, the center value of each band and the number of classified pixels were compared, and result images were examined by pixel to pixel comparison. It was found that care should be taken to avoid false sharing of OpenMP in intra-node implementation. To process large satellite images in a PC-cluster, code and hardware should be designed to reduce performance degradation caused by file I / O. Also, it was found that performance can differ depending on the operating system installed in a PC-cluster.

Effects of Incompatibility on Protoplast Fusion between intra-and inter Species in Basidiomycete, Pleurotus spp. (느타리버섯의 불화합성(不和合性)이 종내(種內) 및 종간(種間) 원형질체(原形質體) 융합(融合)에 미치는 영향(影響))

  • Go, Seung-Joo;You, Chang-Hyun;Shin, Gwan-Chull
    • The Korean Journal of Mycology
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    • v.17 no.3
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    • pp.137-144
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    • 1989
  • Effects of incompatibility existing between intra-and interspecies in Pleurotus spp. on protoplast fusion, clamp formation of their fusants and fruitbody production were investigated. Protoplast fusion between intra-and interspecies of the fungus was achieved by Poly ethylene glycol treatment. The fusion frequency between intraspecies was a little higher than that of interspecies. Fusion frequency between interspecies was not correlated with their similarities based on isozyme patterns. In case of protoplast fusion between intra-and interspecies, the fusants from the compatible isolates produced normal fruit bodies, while those from the incompatible isolates did not produce clamp connections and fruit bodies except those of a few isolates presumed mutants.

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Feasibility and reliability of various morphologic features on magnetic resonance imaging for iliotibial band friction syndrome

  • Jin Kyem Kim;Taeho Kim;Hong Seon Lee;Dong Kyu Kim
    • The Korean Journal of Pain
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    • v.36 no.2
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    • pp.208-215
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    • 2023
  • Background: To evaluate the feasibility, inter-reader reliability, and intra-reader reliability for various morphological features reported to be related to iliotibial band friction syndrome (ITBFS) on knee magnetic resonance imaging (MRI). Methods: A total of 145 patients with a clinical diagnosis and knee MRI findings consistent with ITBFS were included in the "study group" and 232 patients without knee pathology on both physical examination and MRI were included in the "control group". Various morphologic features on knee MRI were assessed including the patella shape, patella height, lateral epicondyle anterior-posterior (AP) width, lateral epicondyle height, ITB diameter (ITB-d), and ITB area (ITB-a). Results: Patients in the study group had significantly higher lateral epicondyle height (13.9 mm vs. 12.92 mm, P = 0.003), ITB-d (2.9 mm vs. 2.0 mm, P = 0.022), and ITB-a (38.5 mm2 vs. 23.8 mm2, P < 0.001) than the control group. ITB-a showed higher area under the curve index (0.849 with 74.1% sensitivity and 72.4% specificity at a 30.3 mm2 cutoff) than ITB-d (0.710 with 70.8% sensitivity and 61.2% specificity at 2.4 mm cutoff) and lateral epicondyle height (0.776 with 72.4% sensitivity and 67.8% specificity at 13.4 mm cutoff). However, only the inter-reader agreement for ITB-a (intraclass correlation coefficient = 0.65) was moderate, while the agreements for other morphologic features were good or excellent. Conclusions: Lateral epicondyle height seems to be a reliable and feasible morphologic feature for diagnosis of ITBFS.

OUTAGE PROBABILITY ANALYSIS OF OC-CDMA SYSTEM WITH IMPERFECT POWER CONTROL UNDER DIFFERENT VELOCITY USERS

  • Panya, Muangruen;Junnapiya, Somyot;Techotchawan, Amnouy;Kidakorn, Pongjai;Omsin, Somchai
    • 제어로봇시스템학회:학술대회논문집
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    • 2004.08a
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    • pp.480-483
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    • 2004
  • This paper, we present the evaluation of outage probability on the SNR under the overlapped carrier allocation scheme in the reverse link with imperfect power control of a cellular CDMA system, which is base on using Gaussian approximation. In numerical results, the band limited pulse generated by square-root of raised consin pulse shaping filters (SRRC) of the transmitted signal is investigated and compared in conventional FD/CDMA and OC-CDMA of the outage probability. It will be also show that the outage probability can be improved by overlapping of carriers. We use error statistics to model the intra-cell interference and evaluate the impact of different mobiles velocity and number of resolvable paths on the system performance.

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Self-Organized Resource Allocation for Femtocell Network to Mitigate Downlink Interference

  • Sable, Smita;Bae, Jinsoo;Lee, Kyung-Geun
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.40 no.12
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    • pp.2410-2418
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    • 2015
  • In this paper, we consider the femto users and their mutual interference as graph elements, nodes and weighted edges, respectively. The total bandwidth is divided into a number of resource blocks (RBs) and these are assigned to the femto user equipment (FUEs) using a graph coloring algorithm. In addition, resources blocks are assigned to the femto users to avoid inter-cell interference. The proposed scheme is compared with the traditional scheduling schemes in terms of throughput and fairness and performance improvement is achieved by exploiting the graph coloring scheme.

Probing Tidal Field Strength of Virgo Cluster Galaxies

  • Yoon, Hye-In;Chung, Ae-Ree
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.2
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    • pp.74.2-74.2
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    • 2011
  • We probe the tidal perturbation parameter f of Virgo cluster galaxies. The goal is to measure the strength of tidal fields around individual galaxies to get better understanding gravitational processes that can affect galaxy evolution in the cluster environment. The f-value is defined as a logarithmic ratio between the net internal gravitational force within a galaxy and the external tidal force exerted by a neighboring galaxy. Hence, it provides one way to quantify the tidal field strength of galaxies, in particular, due to galaxy neighbors. In this study, we determine f-values of the VIVA galaxies, samples of the VLA Imaging study of Virgo galaxies in Atomic gas, using the Extended Virgo Cluster Catalog (EVCC) which is the most complete Virgo cluster catalog to date. With diagnostics based on the HI gas and R-band morphologies, we discuss the impact of the tidal fields on the evolution of the VIVA sample. Also, we compare the tidal field strength to the intra cluster medium (ICM) pressure for each sample galaxy to pin down environmental processes at work.

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