• BMB Reports
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• v.40 no.4
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• pp.577-587
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• 2007

Single-copy chloroplast loci are used widely to infer phylogenetic relationship at different taxonomic levels among various groups of plants. To test the utility of chloroplast loci and to provide additional data applicable to hybrid evolution in Musa, we sequenced two introns, rpl16 and ndhA, and two intergenic spacers, psaA-ycf3 and petA-psbJ-psbL-psbF and combined these data. Using these four regions, Musa acuminata Cola(A)- and M. balbisiana Colla (B)-containing genomes were clearly distinguished. Some triploid interspecific hybrids contain A-type chloroplasts (the AAB/ABB) while others contain B-type chloroplasts (the BBA/BBB). The chloroplasts of all cultivars in 'Namwa' (BBA) group came from the same wild maternal origin, but the specific parents are still unrevealed. Though, average sequence divergences in each region were little (less than 2%), we propose that petA-psbJ intergenic spacer could be developed for diversity assessment within each genome. This segment contains three single nucleotide polymorphisms (SNPs) and two indels which could distinguish diversity within A genome whereas this same region also contains one SNP and an indel which could categorize B genome. However, an inverted repeat region which could form hairpin structure was detected in this spacer and thus was omitted from the analyses due to their incongruence to other regions. Until thoroughly identified in other members of Musaceae and Zingiberales clade, utility of this inverted repeat as phylogenetic marker in these taxa are cautioned.

• Journal of Plant Biology
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• v.32 no.3
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• pp.181-188
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• 1989

C-banded karyotypes in A. anisopodium, A. sacculiferum, A. deltoide-fistulosum and A. splendens were investigated. The chromosome compositions were diploid of 2n=16 in A anisopodium and A. splendens, and teiraploid of 2n=32 in a. sacculiferum and A. deltoide-fistulosum. The bands were distributed in telomeric parts of the chromosomes dominantly in addition to interstitial regions sporadically, resulting in the specific C-banded karyo types according to the species. No centromeric band was observed in these species except only one chromosome in A. deltoidefistulosum. The interspecific relationship based on the C-band distribution will be discussed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.1
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• pp.66-69
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• 2000

The common harpacticoida copepod species Tigriopus japnnicus was often obseued in the mass culture of marine rotifer tanks of sea farming stations. In this study, we investigated the co-existing effect of T. iaponicus to the rotifer Brachionus rotundiformis population growth. The culture conditions as temperature, salinity, culture volume, photo period, culture period and observation interval were 25{\circ}C, 22ppt, 40ml, 12L:12D, 16 deys$ and every two days during the experimental period, respectively. Nannochloropsis oculata was used as the food for the two testing organisms. After counting the growth of rotifer and copepod, they were transferred to new culture tank with N. oculata at the density of $7{times}10^5\;cells/ml$. The population growth of B. rotundiformis was suppressed by T. japonicus when these two organisms were mix-cultured comparing to single culture of B. rotundiformis. But population growth of T, japomcus was not affected by the presence of B. rotundiformis. The interspecific relationships of prey-predation and food competition were not observed in the combination culture of rotifer B. rotundiformis and copepod T japonicus.

• Journal of Plant Biology
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• v.33 no.1
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• pp.1-24
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• 1990

This study was attempted to clarify the taxonomic problems that have been raised up to now in the study o section Telephium, genus, Sedum: the delimitation of taxa belong to Telephium group, their relationship, and nomenclatural confusion and the rank of Telephium group. Specimens were collected at 19 sites in Korea. The habitat, distribution, external morphological characters of habit, leaf shape, phyllotaxy, inflorescence, color of floral organs, floral formula, carpel, capsule, and micro-characters of pollen, seed coat, stigma and anther with SEM were examined. Through this study, the following results were obtained. The delimitation of taxa that belong to section Telephium in Korea and their relationship Nomenclatural errors were corrected. In S. rotundifolium Lee, species epithet“rotundifolium”is later homonym of s. rothundifolium Lamarck, so corrected to S. duckbongii Chung and Kim by article 64 of ICBN. Phyllotaxy was elucidated to meaningless, character in sect. Telephium. S. taquetii was a separate species from S. viridescens and not of its synonym. S. alboroseum Baker in Korea was S. erythrostictum sensu Master not of S. erythrostictum Miquel. By this study , taxa of sect. Telephium in Korea were arranged to 7 species. Considering interspecific relationship of sect. Telephium, S. duckbongii and S. alboroseum are considered to be more advanced forms and S. viridescens, S. taquetii, and S. viviparum to be more primitive forms among 7 species.

• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.30-34
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• 2005

Polygonatum species are a useful medical crop in Korea but basic study in the species was not well conducted. The study was carried out to analyse genetic diversity and intraspecific relationship of 47 Polygonatum species collected from Gyeongnam province. Their analysis was done through principle component analysis and average linkage cluster analysis with their twelve morphological traits. The result of principle component analysis showed the Prin 1, Prin 2 and Prin 3 represented 79% of total variation. By the 0.7 average distance of the cluster analysis and the calculated Euclidian distance, the 47 collected species were grouped into five groups. Group I included 22 collected species representing P. ordoratum var. pluriflorum, group II did 5 ones representing P. involucratum, group III was divided into two subclasses, 2 species including P. inflatum and 7 species including P. thunbergii, group IV also consisted of 2 subclasses, a species similar to P. thunbergii and P.involucratum, respectively, and finally group V included 8 species representing P.lasianthum var. coreanum. meaning that the useful germplasms can be collected from relatively small area.

• The Korean Journal of Ecology
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• v.15 no.2
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• pp.103-115
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• 1992

This dissertation, which has been prepared in the region of cheju(33o31'N, 126o32'E) from may,1987 to appril, 1989, is to elucidate the dominant characteristics of early stages in secondary succession, by examining the life history of erigeron annuus for the seeding depth of a seed was dependent upon the degree of shading. That is, on the area of ocm,the more tense the shading was, the more germination percentage was increased, while, in the case of 1.5cm, it was somewhat increased, when shading bwcame ess and less tense. The rosette of e. annuus, through its relative light intensity was decreased by shading, up to 5% of natural light, showed some strong shade tolerance, which had no difficulty in matter production. The species diversity of mixed-species community was the highest in the middle of April, the lowest in the last of may. The dominance index was the highest in the late may, the lowest in the middle of April. Toward the end of may, the occupation rate of dominance species was the highest. There was a neutral relationship between intraspecific and interspecific, owing to the differentiation of its niche, temporally and spatially. Productive structure of the community revealed a narrow leaf type which was concentratively distributed in the mid part of community height. The relative light intensity of community ground surface was 6.1%, the leaf inclination 60o,the extinction coefficient(k) 0.4, biomass of community 1,045.6 g.d.w./m2,T/Rratio 9.3, C/Frate 7.0 and sumgermanition, shade toarance and community characteristics on erigeron annuus l.in chejumed leaf area index 3.88. through the various life cycles o e.annuus, we can say that it decreases mortality of seeding caused by some stress and disturbance, for germination lasts for a long time at any opportunity available, and it promotes population growth. The strong shade tolerance of a rosette and the variableness of a life from differentiate the niche between intraspecific and interspecific within the community, and avoid the direct competition between them, thus poromoting community growth.

• Korean Journal of Ichthyology
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• v.12 no.4
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• pp.223-229
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• 2000

Phylogenetic relationships between 8 species Korean loaches (Cobitidae) were investigated by comparing mitochondrial cytochrome b gene sequences. However our results are in accordance with finding observed using other morphological studies, new interesting interspecific variation in Korean loaches were found. Orthrias and Lefua appeared to be paraphyletic in Cobitidae observed. Their sequence divergence value was agreed with interfamilic sequence divergences between Cobitidae and Cyprinidae ranged from 0.184 to 0.272. Otherwise, the present results support that two species of Iksookimia and Cobitis melanoleuca were early diverged respectively. And another remarkable result was sequence divergence between Misgurnus anguillicaudatus from China and M. anguillicaudatus from Yongdok, Korea. That was 0.099, which was interspecific value. Also the phylogenetic location of some Iksookimia species was suggested as the cobitid intergeneric hybrid origin.

• Journal of Forest and Environmental Science
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• v.39 no.1
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• pp.27-39
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• 2023

Twenty-five Eucalyptus clones (14 E. camaldulensis - EC and 11 interspecific eucalypt hybrid clones - EH) grown in three contrasting sites were evaluated for the growth and few wood traits at 4 years of age. The stability, genotype-site interaction and suitability of these clones for pulp and solid wood industry sectors were studied. Growth of eucalypt clones was significantly higher at site 1 with higher rainfall, but wood density did not differ significantly from lower rainfall sites. Kraft pulp yield (KPY) decreased from sites 1 to 3 based on moisture availability, but not between two groups of clones. Volumetric shrinkage (VS) was significantly higher in EC clones at site 3 with lowest rainfall, but there was no specific trend at other two sites with maximum (site 1) and intermediate (site 2) rainfall. The mechanical traits modulus of rupture (MOR) and modulus of elasticity (MOE) were at par in sites 1 and 2, but significantly lower at the driest site 3. The growth rate had a significant positive correlation with KPY, MOR and MOE and a negative correlation with VS, but no significant impact on wood density in both groups of clones. Genotype×environment interaction (G×E) was evident in most traits due to the difference in response of clones to moisture availability. Since wood density was negatively correlated to KPY, it has to be kept at an optimum level for the profitability of pulp industry. There was no significant difference between EC and EH clones for most traits except VS at site 3. Stability of clones varied across sites in different traits, and hence clones may be selected for deployment at each site by screening for growth, followed by wood density, considering the relationship of growth and density with other traits required by pulp and solid wood industry sectors.

• The Korean Journal of Mycology
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• v.26 no.2 s.85
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• pp.163-172
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• 1998

Thirty six strains of Pleurotus spp., from world-wide nations, were examined for interspecific isozyme variation. A comparison of isozymes in mycelial extracts of the fungal genus Pleurotus was made by polyacrylamide gel isoelectric focusing. A total of one hundred and sixty six bands was resolved from six isozymes. A cluster analysis was done based on the zymograms for esterase, glucosephosphate isomerase, leucine aminopeptidase, malate dehydrogenase, peroxidase, and phosphoglucomutase. From the isozyme analysis, esterase showed higher degree of variability, while it was observed less variability for the enzymes such as glucosephosphate isomerase, malate dehydrogenase, and phosphoglucomutase. The species P. ostreatus, whose taxon is controversial, was discriminated from P. pulmonarius, while P. florida was classified as a distinct taxon. The clustering of P. sapidus and P. spodoleucus strains appeared to be more difficult. It was found that some strains were included to another cluster based on electrophoretic banding patterns. These results show that this lack of congruence among data sets may help explain the taxonomic difficulty within the genus Pleurotus. A dendrogram of genetic similarities was presented, and applications of isozyme data to the systematics of these commercially important fungi was discussed.

• The Korean Journal of Ecology
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• v.21 no.3
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• pp.301-307
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• 1998

Intraspecific and interspecific isozyme variations and their relationship of 16 local populations in 3 species of Phyllostachys, that is, P. bambusoides, P. nigra var. henonis and P. pubescens were investigated by multi-variate analysis. Leaf isozymes of Phyllostachys such as 6-PGD (6-phosphogluconate dehydrogenase), MDH (malate dehydrogenase), PGI (phosphoglucoisomerase), PRX (peroxidase), PGM (phosphoglutamase), IDH (isocitrate dehydrogenase) showed electrophoretic variations in the number of zymotypes (7, 6, 6, 9, 3 and 5, respectively). In the cluster analysis on the isozymic characteristics, 16 populations were classified into 3 species at the euclid genetic distance of 2.041. P. nigra var. henonis and P. bambusoides were clustered first at 2.813 and then P. pubescens at 3.001. So far, 3 local types of intraspecific ariation were found in P. nigra var. henonis and P. bambusoides.