• The Plant Pathology Journal
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• v.29 no.1
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• pp.17-30
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• 2013

Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. Latrunculin B (LatB) treat-ments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed: Talin fluorescence in these plants colocalized with the ER vesicles. TGB3 also associated with the Golgi apparatus and overlapped with cortical vesicles appearing at the cell periphery. Brefeldin A treatments disrupted Golgi and also altered vesicles at the CW, but failed to interfere with TGB CW localization. Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.60-60
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• 2018

This study was carried out to develop the seed propagation method of Dicenta spectabilis (L.) Lem. which is an ornamental plant native to Korea. In the previous studies, it was found that the seeds of D. spectabilis were morphophysiologically dormant (MPD), and high and low temperature of stratification were continuously required for the embryo growth and germination of the seeds. Especially, it was most effective to store for 1 month at $20^{\circ}C$ and then to transfer to $4^{\circ}C$. The treatment of $GA_3$ was carried out to promote embryo development and germination. The seeds were submerged in 100, 200 or $500mg{\cdot}L^{-1}$ $GA_3$ for 72 hours and then stored at various conditions as follow. The temperature conditions disposed of this experiment were 1 month at 10, 15, 20, and $25^{\circ}C$ or 2, 4, 8, and 12 months at $4^{\circ}C$, respectively. As a result, the length of embryo and germination rate of the seeds were the best when stored at $4^{\circ}C$ for 8 months after $500mg{\cdot}L^{-1}$ $GA_3$ treatment. Besides, when the seeds stored at $4^{\circ}C$, significant differences in embryo length and germination rate were shown with $GA_3$ concentration and storage period. It was also proved that high-concentration of $GA_3$ could replace the high temperature and could promote germination. Consequentially, the D. spectabilis seeds were classified into intermediate simple levels among MPD types.

• Proceedings of the Botanical Society of Korea Conference
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• 1987.07a
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• pp.191-211
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• 1987

Common usage of the concept of juvenility implies that there is one physiological phase, the juvenile phase, which manifests itself in the various morphological and physiological phenomena observed in juvenile higher plants. The juvenile phase is often defined as that time from seed germination until the plant attains the ability to flower regulating such behaviour. This definition precludes plants from flowering in the juvenile phase. It is of major interest, therefore, to identify the physiological controls(Bluehreife) regulating such behavior. The length of the juvenile period in higher plants ranges from one year to over 60 years in different species. The long juvenile period of seedling is the main cause of the long duration of the breeding process. I determined the length of the juvenile period in various plants and its control of phase changes in natural system in relation to factors such as plant size and age, shoot morphology, apex size, root system and phytohormonal and nutritional status is reviewed. From the own experimental and observational evidence available it appears that both hormonal and nutritional factors can be involved in control of juvenility but that a specific juvenile or flowering hormone is not involved. Grafting, ringing, scoring, root pruning and fertilization have been used to accelerate flowering, but in most cases these cultured treatments are only successful on plants that were passed the juvenile phase. It is suggested that there are intrinsic difference between the meristematic cells of the apieces of juvenile and adult shoot, which are thus determined with respect to there development potentialities. The problems associated with the maintenance of the determined state through mitosis are discussed. The properties of transitional forms of Ribes nigrum L. intermediate between the juvenile and adult phase, are descrived and there implications discussed. Analogies are drawn between juvenile phenomena in woody perennials and in herbaceous species.

• Korean Journal of Breeding Science
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• v.41 no.2
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• pp.101-105
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• 2009

Rice (Oryza sativa L.), the world's most important crop, is usually classified into ssp. indica and japonica based on morpho-physiological traits. In the previous study, we have developed subspecies-specific STS markers (SS markers) to readily discriminate between indica and japonica in O. sativa. In this study, we employed SS markers to investigate the genomic inclination of worldwide collections of O. sativa. A total of 320 varieties were divided into two groups with 63 SS markers. Namely, they formed two distinctive groups, indica and japonica, as expected by their geographic origin. The population structure analysis revealed that the variability of genetic background was greater in indica than in japonica. Some of them, however, exhibited intermediate genomic inclination between indica and japonica. These results are in general agreement with the previous studies, suggesting that SS markers are powerful tools for both determination of subspecies genome and assessment of genetic diversity in rice.

• Korean Journal of Plant Taxonomy
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• v.31 no.3
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• pp.253-265
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• 2001

Cymopterus melanotilingia (Apiaceae) was reviewed by external morphology and cross-section of leaf-margin and mericarp, and the taxonomic position of species and genus was reexamined. Based on these results, the description and adistribution map of C. melanotiligia were obtained. The leaf-margin forms a collenchymatous ridge by projecting toward the lower part. One mericarp has 2-lateral ribs and 2-intermediate ribs and the other mericarp has 2-lateral ribs and 1-dorsal rib, suggesting each of them is asymmetric. These characters show that this species does not belong to the genus Ostericum s.s., but should be treated as the genus Cymopterus.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.124-124
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• 2003

Citrus canker is very important disease in international trade of citrus. The disease was usually take place from late of June, and severe middle of July to middle of August, though disease occurrence was affected by environmental conditions. In pathogenicity test, three varieties, orange, lemon and kiyomi among 7 varieties, were succeptible, two varieties, satsuma mandarin and iwasachi, intermediate resistant. On the other hand, shiranuhi and yuzu were resistant relatively. The pathogen, Xanthomonas axonopodis pv. citri, grew well in PD broth adjusted to pH 7.0 at 26$^{\circ}C$. It's growth was best in medium containing group of monosaccharide as a carbon source and group of ammonium as a nitrogen source. Tow isolates were resistant to streptomycin among 11 isolates isolated from diseased leaves in field in Jeju-Do. The streptomycin sensitives isolate was controlled by in greenhouse test. On the other hand, the resistant and sensitive isolates were controlled by treatment with copper sulfate, the control value is 88.7％ and 90.6％, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.4
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• pp.593-601
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• 2012

Heavy metals and metalloids removal can be considered as one of the most important world challenges because of their toxicity and direct impact on human health. Many processes have been introduced but biological processes of remediation seem to offer the most suitable solution in terms of efficiency and low cost. Actinobacteria constitute one of the major microbial populations in soil, and this can be attributed to their adaptive morphological structure as well as their exceptional metabolic power. Among microbes, actinobacteria are morphologic intermediate between fungi and bacteria. Studies on microbial diversities in metal contaminated lands have shown that actinobacteria may constitute a dominantly active microbiota in addition to ${\alpha}$ Proteobacteria. Furthermore, isolation studies have shown metal removal mechanisms which are reminiscent of notable multiresistant strains, such as Cupriavidus metallidurans. Apart from members of genus Streptomyces, which produce more than 90% of commercialized antibiotics, and the nitrogen fixing Frankia, little attention has been given to other members of this phylum. This is because of difficult culture condition requirements and maintenance. In this review, we focused on specific isolation of actinobacteria and their potential applications in metal bioremediation and plant growth promotion.

• Plant Journal
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• v.13 no.1
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• pp.30-36
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• 2017

During the start-up of 500 MW class coal-fired power plant, abnormal shaft vibration was occurred on bearings installed on both side of high and intermediate pressure steam turbine. Shaft vibration was analyzed to investigate the reason and find the resolution, based on well-known theory in this study. Typical vibration characteristics which occur when rotating parts contact with stationary parts were observed at the analysis of frequency, amplitude and phase angle. The reason of abnormal vibration was assumed to be rub and internal parts wear was observed during repair period. As a result of applying low speed turning and balancing for resolution of abnormal vibration, balancing was more effective for rub removal. So balancing could be excellent resolution in the case of abnormal vibration which is similar to this study.

• Journal of Plant Biology
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• v.31 no.3
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• pp.169-185
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• 1988

Vessel elements in lateral root, tap root, transition region, stem and mid vein of 1-year old, 3-year old and 5-year old ginseng (Panax ginseng C.A. Meyer) are studied with light microscope to clarify the distribution and differentiation of several kinds of vessel elements. Vessel elements are classified into five types such as ring vessel, spiral vessel, scalariform vessel, reticulate vessel and pitted vessel according to the secondary thickenings of cell wall. All of the five types are not observed in each organ, but diverse kinds of vessels are present in stem and mid vein compared with the underground organs such as tap root and lateral root. The length of vessel elements is longest (680$\mu$m) in stem and shortest (143$\mu$m) in tap root. The diameter of vessel elements is 19.0$\mu$m in tap root and the angle of perforation plate comes under 22$^{\circ}$-60$^{\circ}$. The degree of differentiation of vessel elements according to the length, diameter and angle of perforation plate of vessel elements is highest in tap root regardless of the age of ginseng. Three types of perforation plate such as scalariform, intermediate type of simple and scalariform, and simple perforation plate are observed. The vascular tracheids are characteristically observed in mid vein of 1-year old ginseng, and in transition region of 3 and 5-year old ginseng.

• Journal of Plant Biology
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• v.8 no.1_2
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• pp.11-18
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• 1965

Since Taquet's first discovery of wild P. yedoensis at Mt. Halla, Korea, in 1908, its morphological chracteristics and question of origin of cultivated yedoensis have given controversies to the botanists. Takenaka, through his experiments on the hybridity of cultivated P. yedoensis, recently holds the opinion that P. yedoensis might have originated in Izu peninsula, Japan. The author presents the summarized report on the wild P. yedoensis and its putative parents based on his 2 years' investigated carried out at Mt. Halla during his breeding experiment of genus Prunus. The species of cherry tree used in the present investigation were identified by Prof. Mankyu Park and Mr. Jonghyu Pu, Korean taxonomists. 1) Wild cherry trees which grow wild in Mt. Halla and whose blooming season is April are mostly P. subhirtella var. pendula form. ascendens and P. donarium P. yedoensis is rare in number, around 10 individuals, having been found in a half century. 2) Individuals of wild yedoensis are variable in some of their morphological characteristics. This is, also, true in other species of Prunus. 3) Wild yedoensis whose vigorous growth, sterility, and rarity in number suggest hybrid origin, has intermediate characteristics between the P. subhirtella and P. donarium. 4) Due to the abnormal weather of the island and various environmental factors such as havbitats, some of the early-blooming subhirtella and late-blooming donarium would bloom at the same time, giving these two species the changes to cross. 5) Wild yedoensis is slightly different in some of its quantitative characters from cultivated species.