• Food Science and Biotechnology
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• 제14권6호
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• pp.847-853
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• 2005

A total of 60 growth curves were generated with combinations of temperature, pH, and potassium lactate (PL) (60% (v/v) commercial solution) to determine the lag time (LT) and specific growth rate (SGR) of L. monocytogenes in broth. LT and SGR were significantly (P<0.05) affected by temperature, pH, concentration of PL, or the combined interaction of these factors. LT was extended and SGR was reduced significantly (P<0.05) by increased concentration of PL at lower temperature and pH. Listericidal effect was observed in the broth containing 2, 3, and 4% PL at pH 5.0 and $4^{\circ}C$. The antimicrobial activity of PL against L. monocytogenes increased when the pH of the medium was decreased at all temperatures tested. The results suggest that PL has antimicrobial properties to suppress the growth of L. monocytogenes. Potassium lactate has many potential applications as an antimicrobial additive in variety of refrigerated ready-to-eat foods.

• Bulletin of the Korean Chemical Society
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• 제35권1호
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• pp.197-203
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• 2014

A rapid, accurate and precise method for the determination of 22 amino acids in Isatidis Radix by Hydrophilic Interaction Ultra-High-Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry (HILIC-UPLC-MS/MS) was established. Chromatographic separation was carried out on a Acquity UPLC BEH Amide column ($2.1mm{\times}100mm$, $1.7{\mu}m$) with gradient elution of acetonitrile (containing 0.05% formic acid and 2 mM ammonium formate) and water (containing 0.15% formic acid and 10 mM ammonium formate) at a flow rate of 0.4 mL/min; Waters Xevo$^{TM}$ TQ worked in multiple reaction monitoring mode. All components were separated in 17 min. All calibration curves were linear ($R^2$ > 0.991) over the tested ranges. The limits of detection (LOD) and limits of quantitation (LOQ) for these compounds were 0.21-79.55 and 0.72-294.23 ng/mL, respectively. The average recoveries were in the range of 93.75-104.16% with RSD value less than 6.56%. Therefore, this method could be an alternative assay for the determination of 22 amino acids in Isatidis Radix due to its rapidness, sensitivity, less sample and solvent consumption.

• Bulletin of the Korean Chemical Society
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• 제27권12호
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• pp.2040-2044
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• 2006

Dielectric studies of methyl acrylate with 1-propanol, 1-butanol, 1-heptanol and 1-octanol binary mixtures have been carried out over the frequency range from 10 MHz to 10 GHz at temperatures of 283, 293, 303 and 313 K using Time Domain Reflectometry (TDR) for various concentrations. The Kirkwood correlation factor and excess inverse relaxation time were determined and discussed to yield information on the molecular structure and dynamics of the mixture. The values of the static dielectric constant, relaxation time and the Kirkwood correlation factor decrease with increased concentration of methyl acrylate in alcohol. The Bruggman plot shows a non-linearity of the curves for all the systems studied indicates the heterointeraction which may be due to hydrogen bonding of the OH group of alcohol with C=O of the methyl acrylate. The excess inverse relaxation time values are negative for all the systems at all the temperatures indicates that the solute-solvent interaction hinders the rotation of the dipoles of the system.

• Bulletin of the Korean Chemical Society
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• 제30권5호
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• pp.1021-1025
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• 2009

A novel supramolecular network containing binuclear copper unit $[Cu(phen)_{2}({\mu}-ID\;A)Cu(phen){\cdot}(NO_{3})](NO_{3}){\cdot}4(H_{2}O)$ (1) was synthesized through the self-assembly of iminodiacetic acid ($H_2IDA$) and 1,10-phenanthroline (phen) in the condition of pH = 6. It has been characterized by the infrared (IR) spectroscopy, elemental analysis, single crystal X-ray diffraction, and thermogravimetric analysis (TGA). 1 shows a 2-D supramolecular structure assembled through strong and unique $\pi-\pi$ packing interactions. Density functional theory (DFT) calculations show that theoretical optimized structures can well reproduce the experimental structure. The TGA and powder X-ray diffraction (PXRD) curves indicate that the complex 1 can maintain the structural integrity even at the loss of free water molecules. The magnetic property is also reported in this paper.

• International Journal of Fluid Machinery and Systems
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• 제5권4호
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• pp.161-167
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• 2012

Small-sized axial fans are used as air coolers for electric equipment. There is a strong demand for higher power of fans according to the increase of quantity of heat from electric devices. Therefore, higher rotational speed design is conducted, although, it causes the deterioration of the efficiency and the increase of noise. Then, the adoption of contra-rotating rotors for small-sized fans is proposed for the improvement of the performance. In the case of contra-rotating rotors, blade row distance between the front and the rear rotors influences on the performance and the noise. Therefore, it is important to clarify the optimum blade row distance between front and rear rotors. The performance curves of the contra-rotating small-sized axial fan under the condition of different blade row distances are shown and the blade row interaction between the front and the rear rotors are discussed by the numerical results. Furthermore, the optimum blade row distance of the contra-rotating small-sized axial fan is considered.

• BMB Reports
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• 제28권6호
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• pp.471-476
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• 1995

Acetolactate synthase (ALS) catalyzes the first common step in the biosynthesis of valine, leucine, and isoleucine. ALS is the target enzyme for several classes of structually diverse herbicides. We have synthesized 4,6-dimethoxypyrimidine derivatives as ALS inhibitors, and their inhibitory activities on barley ALS were determined. $IC_{50}$ values for the derivatives are 0.2~200 ${\mu}m$. K11570, the most potent ALS inhibitor with $IC_{50}$ of 0.2 ${\mu}m$, showed mixed-type inhibition with respect to substrate pyruvate, and the progress curves for ALS inhibition by K11570 indicated that the amount of inhibition increased with time. Inhibition-competition experiments were carried out and indicated that three different classes of inhibitors, K11570, a sulfonylurea Ally, and leucine, bind to ALS in a mutually exclusive manner. Chemical modification of tryptophanyl and tyrosyl residues of ALS decreased the sensitivity of ALS to K11570, while cysteine modification did not affect the sensitivity. These results suggest that tryptophanyl and tyrosynyl residues are probably located at or near the inhibitor binding site.

• Computers and Concrete
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• 제15권3호
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• pp.411-436
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• 2015

In this work, a comprehensive approach to model the structural behaviour of Reinforced Concrete (RC) beams subjected to reinforcement corrosion is proposed. The coupled environmental - mechanical damage model developed by some of the authors is enhanced for considering the main effects of corrosion on concrete, on composite interaction between reinforcement bars and concrete and on steel reinforcement. This approach is adopted for reproducing a set of experimental tests on RC beams with different corrosion degrees. After the simulation of the sound beams, the main parameters involved in the relationships characterizing the effects of corrosion are calibrated and tested, referring to one degraded beam. Then, in order to validate the proposed approach and to assess its ability to predict the structural response of deteriorated elements, several corroded beams are analyzed. The numerical results show a good agreement with the experimental ones: in particular, the proposed model properly predicts the structural response in terms of both failure mode and load-deflection curves, with increasing corrosion level.

• Structural Engineering and Mechanics
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• 제62권4호
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• pp.381-389
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• 2017

In the present study, the advanced procedure has been proposed to estimate higher accuracy of embedment of pipes that are installed on soft clay seabed. Numerical simulation by OrcaFlex simulation code was performed to investigate dynamic seabed embedment, and two steps, i.e., static and dynamic analysis, were adopted. In total, four empirical curves were developed to estimate the seabed embedment including dynamic phenomena, i.e., behaviour of vessel, environmental condition, and behaviour of nonlinear soil. The obtained results were compared with existing methods (named general method) such as design code or guideline to examine the difference of seabed embedment for existing and advance methods. Once this process was carried out for each case, a diagram for estimating seabed embedment was established. The applicability of the proposed method was verified through applied examples with field survey data. This method will be very useful in predicting seabed embedment on soft clay, and the structural behaviours of installed subsea pipelines can be changed by the obtained seabed embedment in association with on-bottom stability, free span, and many others.

• Archives of Pharmacal Research
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• 제2권1호
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• pp.49-64
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• 1979

In an atempt to elucidate further physicochemical properties of furosemide-PVP coprecipitates, extensive investigations such as TLC, UV,IR, NMR, X-ray diffraction, TGA and DTA studies were carried out for the furosemide test systems. X-ray diffraction studies revealed that the pure furosemide and the furosemide contained within a physical mixture were crystalline in nature. However, there was no crystallinity evident in the 1:5 furosemide-PVP 40,000 coprecipitate system, even after standing for two years. The various ratio furosemide-PVP 40,000 coprecipitate systems revealed that the coprecipitate containing a greater amount of PVP 40,000 than that of furosemide showed a crystalline state of furosemide and that the minimum amounts of PVP to make amorphous form of furosemide was 1:1 ratio of furosemide to PVP. From the furosemide-PVP coprecipitate systems with PVP of different molecular weights of 10,000, 40,000 and 360,000, all the 1:1 ratio coprecipitates did not exhibit any crystallinity of furosemide, whereas all the 2:1 ratio coprecipitates showed a presence of crystalline furosemide. All the coprecipitated preparations with PEG 4,000 and with PEG 6,000 showed the diffraction peaks indicating the presence of crystalline furosemide. The comparison of infrared spectra of the physical mixture and the coprecipitate showed an interaction such as association between the functional groups of furosemide and PVP in the molecular level, whereas the studies by TLC, UV and NMR showed its dissociation in methanol solution. The weight losses in TGA curves showed all the same patterns. However, a little different transition form in DTA thermograms was shown between the physical mixture and the coprecipitate, indicating the different thermal property.

• 콘크리트학회논문집
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• 제11권3호
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• pp.89-100
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• 1999

The purpose of this study is to develop an analytic model of the concrete column strengthened with laminated CFS, and to provide a basic guideline for the strengthening design by CFS considering orthotropic properties of laminate. In this study, an analytical stress-strain model of laminated CFS is presented based on Tsai-Hill failure criterion. This model has been implemented in an algorithm which can evaluate the confinement effect of CFS. Through this algorithm, the stress-strain relationship of confined concrete is obtained and compared with experimental results of other studies. Using the constitutive relationships, section analyses of concrete column strengthened with CFS are done, and load-moment and load-curvature interaction curves are obtained. In addition, the strengthening effects of CFS according to various laminated angles are analyzed. Analytical results show that the strengthening effects of the strengthened concrete columns are significantly different in compression, flexure, and ductility according to the laminated ways. In compressive direction of principal stress shows the superiority, where an in flexural strengthening effects, [0/90]s does. In the aspect of ductility, [90]s shows the best effect.