• Title/Summary/Keyword: integrative korean medicine

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Current Status of Occupational Therapy in Korean University Hospital NICUs (국내 대학병원 신생아중환자실 작업치료의 현황)

  • Kim, Sunmi;Park, Seohee;An, Sunjoung
    • Journal of The Korean Society of Integrative Medicine
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    • v.2 no.4
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    • pp.19-27
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    • 2014

  • Purpose : The purpose of this study was to provide some basic information about current status of occupational therapy in Korean University Hospital NICUs. Method : This study was conducted by mail and by analyzing 61 survey questionnaires sent out pediatric occupational therapist in 39 university hospitals. Result : 23 people of the current pediatric occupational therapists worked in NICU under many constraints. Some reasons for not providing occupational therapy services in the NICU were lack of pediatric occupational therapists(55.3%), funding difficulties(42.1%) and problem in the hospital system(42.1%). Conclusion : The results of this study can be used as a basis to increase the provision of occupational therapy in the NICU. Further research is needed to improve the role of occupational therapy in the NICU.

  • https://doi.org/10.15268/ksim.2014.2.4.019 인용 PDF KSCI

Effects of Tumor Microenvironmental Factors on DNA Methylation and Radiation Sensitivity in A549 Human Lung Adenocarcinoma

  • Oh, Jung-Min;Kim, Young-Eun;Hong, Beom-Ju;Bok, Seoyeon;Jeon, Seong-Uk;Lee, Chan-Ju;Park, Dong-Young;Kim, Il Han;Kim, Hak Jae;Ahn, G-One
    • Journal of Radiation Protection and Research
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    • v.43 no.2
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    • pp.66-74
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    • 2018

  • Background: Tumor response to anticancer therapies can much be influenced by microenvironmental factors. In this study, we determined the effect of these microenvironmental factors on DNA methylation using A549 human lung adenocarcinoma cell line. Materials and Methods: We subjected A549 cells to various conditions mimicking tumor microenvironment including hypoxia, acidosis (sodium lactate), oxidative stress ($H_2O_2$), bystander effect (supernatant from doxorubicin (Dox)-treated or irradiated cells), and immune cell infiltration (supernatant from THP-1 or Jurkat T cells). Genomic DNA was isolated from these cells and analyzed for DNA methylation. Clonogenic cell survival, gene expression, and metabolism were analyzed in cells treated with some of these conditions. Results and Discussion: We found that DNA methylation level was significantly decreased in A549 cells treated with conditioned media from Dox-treated cells or Jurkat T cells, or sodium lactate, indicating an active transcription. To determine whether the decreased DNA methylation affects radiation sensitivity, we exposed cells to these conditions followed by 6 Gy irradiation and found that cell survival was significantly increased by sodium lactate while it was decreased by conditioned media from Dox-treated cells. We further observed that cells treated with conditioned media from Dox-treated cells exhibited significant changes in expression of genes including BAX and FAS (involved in apoptosis), NADPH dehydrogenase (mitochondria), EGFR (cellular survival) and RAD51 (DNA damage repair) while sodium lactate increased cellular metabolism rather than changing the gene expression. Conclusion: Our results suggest that various tumor microenvironmental factors can differentially influence DNA methylation and hence radiosensitivity and gene expression in A549 cancer cells.

  • https://doi.org/10.14407/jrpr.2018.43.2.66 인용 PDF KSCI

High-performance liquid chromatography analysis of phytosterols in Panax ginseng root grown under different conditions

  • Lee, Dong Gu;Lee, Jaemin;Kim, Kyung-Tack;Lee, Sang-Won;Kim, Young-Ock;Cho, Ik-Hyun;Kim, Hak-Jae;Park, Chun-Gun;Lee, Sanghyun
    • Journal of Ginseng Research
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    • v.42 no.1
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    • pp.16-20
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    • 2018

  • Background: The Panax ginseng plant is used as an herbal medicine. Phytosterols of P. ginseng have inhibitory effects on inflammation-related factors in HepG2 cells. Methods: Phytosterols (e.g., stigmasterol and ${\beta}$-sitosterol) in the roots of P. ginseng grown under various conditions were analyzed using high-performance liquid chromatography. The P. ginseng roots analyzed in this study were collected from three cultivation areas in Korea (i.e., Geumsan, Yeongju, and Jinan) and differed by cultivation year (i.e., 4 years, 5 years, and 6 years) and production process (i.e., straight ginseng, red ginseng, and white ginseng). Results: The concentrations of stigmasterol and ${\beta}$-sitosterol in P. ginseng roots were 2.22-23.04 mg/g and 7.35-59.09 mg/g, respectively. The highest concentrations of stigmasterol and ${\beta}$-sitosterol were in the roots of 6-year-old P. ginseng cultivated in Jinan (82.14 mg/g and 53.23 mg/g, respectively). Conclusion: Six-year-old white ginseng and white ginseng cultivated in Jinan containing stigmasterol and b-sitosterol are potentially a new source of income in agriculture.

  • https://doi.org/10.1016/j.jgr.2016.10.004 인용 PDF KSCI