• Journal of Microbiology and Biotechnology
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• v.9 no.4
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• pp.488-497
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• 1999

Probing Streptomyces albus ATCC 21838 chromosomal DNA with a proline tRNA sequence resulted in an isolation of a putative integrating element in the 6.4-kb EcoRI fragment. It was found that Streptomyces lividans TK-24 transformed with a cloned DNA fragment on a multicopy plasmid, produced a higher level of spore pigment and mycelial red pigment on a regeneration agar. Furthermore, the transformant S. lividans TK-24 produced a markedly increased level of undecylprodigiosin in a broth culture. A nucleotide sequence analysis of the cloned region revealed several open reading frames homologous to the integrases of integrating plasmids or temperate bacteriophages, signal-transducing regulatory proteins with a conserved ATP-binding domain, oxidoreductases ($\beta$-ketoacyl reductase), and an AraC-like transcriptional regulator. To examine the effect on antibiotic production, each coding region was overexpressed separately from the other genes in the region in S. lividans TK-24 with; pJHS3044 for the expression of the signal-transducing regulatory protein homologue, pJHS3045 for the homologue of oxidoreductase, and pJHS3051 for the homologue of the AraC-like transcriptional regulator. Phenotypic studies of S. lividans TK-24 strains harboring plasmids for the overexpression of individual genes suggested the following effects of the genes on antibiotic production: The oxidoreductase homologue stimulated the production of actinorhodin and undecylprodigiosin, which was influenced by the culture conditions; the homologue of the AraC-like transcriptional regulator was the most effective factor in antibiotic production within all the culture conditions tested; the signal-transducing regulatory protein homologue repressed the effect due to the homologue of the AraC-like transcriptional regulator, however, the antibiotic production was derepressed upon entering the stationary phase.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.8
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• pp.2540-2545
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• 1996

It is well known that in typical displacement-based curved beam elements, the stiffness matrix is overestimated and as a result displacement predictions show gross error for the thin beam case. In this paper, a stain based curved beam element with 2 nodes is formulated based on shallow beam geometry. At the element level, the curvature and membrane strain fields are approximated independently and the displacement fields are obtained by integrating the strain fields. Three test problems are given to demonstrate the numerical performance of the element. Analysis results obtained reveal that the element is free for locking and very effectively applicable to deeply as well as shallowly curved beams.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.31 no.1
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• pp.9-16
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• 2018

When designing a reinforced concrete member using nonlinear finite element analysis results, the bending moment at the critical section should be calculated. In this paper, a bending moment calculation method using the results of reinforced concrete finite element analysis(FEA) using continuum elements is presented and the optimum element size according to the order of the displacement function of the finite element is proposed. The bending moments calculated by integrating the stresses from the FEA are compared with the bending moments calculated using the static equilibrium conditions. In the method of integrating the stress, both the stress due to the reinforcing bar and the stress of the concrete are considered. In addition, various factors affecting the accuracy of the stresses calculated by the FEA were analyzed and the influence of the displacement function and the element size was verified. If the purpose of the analysis is to roughly observe the behavior of the members, it is appropriate to use the first order displacement function and the element size should be about 25% of the section height of the analytical model. When the bending moment of a member with high accuracy is required, it is suggested that the secondary displacement function be used and the element size be 12.5%.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.26 no.10
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• pp.766-770
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• 2013

This paper tried to develop a BLDC electric motor securing the 800 W-level watertight structure for driving the outboard motor. For this purpose, this paper developed a high-efficient controller-integrating BLDC electric motor system for underwater propulsion and designed and developed a triple watertight structure. Besides, this study developed a outboard motor integrating motor, propeller and controller based on the production of a controller for BLDC motor which can the speed control by selecting low-voltage, high-current power element. The characteristics of developed outboard motor were 24 V input voltage, over 800 W motor output, and max. 3,000 rpm motor, and 84.9% motor efficiency, and the developed outboard motor could secure the watertight structure in 5 m in water depth.

• Structural Engineering and Mechanics
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• v.28 no.5
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• pp.587-601
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• 2008

In this study, a numerical procedure based on the finite element method for materially and geometrically nonlinear analysis of reinforced and prestressed concrete slender columns with arbitrary section subjected to combined biaxial bending and axial load is developed. In order to overcome the low computer efficiency of the conventional section integration method in which the reinforced concrete section is divided into a large number of small areas, an efficient section integration method is used to determine the section tangent stiffness. In this method, the arbitrary shaped cross section is divided into several concrete trapezoids according to boundary vertices, and the contribution of each trapezoid to section stiffness is determined by integrating directly the trapezoid. The space frame flexural theory is utilized to derive the element tangent stiffness matrix. The nonlinear full-range member response is traced by an updated normal plane arc-length solution method. The analytical results agree well with the experimental ones.

• Transactions of Materials Processing
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• v.9 no.5
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• pp.478-485
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• 2000

This study aims to Improve the productivity in forming of flanged thrust engine bearings from two kinds of laminated sheet materials by integrating the forming processes or by reducing the number of the subsequent sizing and machining processes or by modifying the forming tools used. For steel-Al rolled blank, a design scheme for the one-step forming operation and the geometry of the tool set required is suggested and is verified its usefulness by the finite element simulation. And for steel-Cu sintered blank, the results of experiment and finite element analysis show that it is possible to improve the dimensional accuracy of formed products and to reduce the number of sizing processes just by modifying the shape and dimensions of initial blanks and flange forming dies, and by controlling the spring force.

• Proceedings of the KSME Conference
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• 2002.08a
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• pp.209-212
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• 2002

Since Berkhoff proposed the mild-slope equation in 1972, it has widely been used for calculation of shallow water wave transformation. Recently, it was extended to give an extended mild-slope equation, which includes the bottom slope squared term and bottom curvature term so as to be capable of modeling wave transformation on rapidly varying topography. These equations were derived by integrating the Laplace equation vertically. In the present study, we develop a finite element model to solve the Laplace equation directly while keeping the same computational efficiency as the mild-slope equation. This model assumes the vertical variation of wave potential as a cosine hyperbolic function as done in the derivation of the mild-slope equation, and the Galerkin method is used to discretize . The computational domain was discretized with proper finite elements, while the radiation condition at infinity was treated by introducing the concept of an infinite element. The upper boundary condition can be either free surface or a solid structure. The applicability of the developed model was verified through example analyses of two-dimensional wave reflection and transmission. .

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2003.10b
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• pp.22-25
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• 2003

The forming limits are studied for cold upsetting of high strength aluminium alloy in the present paper. Different geometry ratio and frictional conditions are investigated in the forgeability test to evaluate the forming limits and also to obtain the various strain paths. The critical fracture value can be obtained by integrating along the strain path till free surface crack initiation. To predict the damage evolution of cold upsetting, the computer-aided evaluation of forming limits is obtained by using the finite-element software DEFORM-3D and the modified Cockcroft-Latham criterion. The predicted theoretical limit strains agree quite well with the experimental results.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2006.04a
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• pp.914-921
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• 2006

To describe the effect of the numerous and various oriented microcracks on the compressive and tensile concrete behaviors, the directional nonlocality is defined. The plasticity model using multiple failure criteria is developed for RC planar members in tension-compression. The crack damages are defined in the pre-determined reference orientations, and then the total crack damage is calculated by integrating multi-oriented crack damages. To describe the effect of directional nonlocality on the anisotropic tensile damage, based on the existing test results, the nonlocal damage factor is defined in each reference orientation. The reduced compressive strength in the cracked concrete is defined by the multi-oriented crack damages defined as excluding the tensile normal plastic strain from the compressive equivalent plastic strain. The proposed model is implemented to finite element analysis, and it is verified by comparisons with various existing panel test results.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1996.10a
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• pp.14-19
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• 1996

This study presents development of finite element simulation program and analysis of hot extrusion through square dies with mandrel. The design of extrusion dies is still an art rather than science. Die design for a new extrusion is developed from through in-plant trials. In the present paper, three-dimensional steady-state finite element simulation program is developed. The developed system is effectively used to simulate extrusion of clamp shaped hollow section. The objective of the present paper is to study the possibility of integrating this method into the design of dies for extrusion of complex section. To obtain sound product, the distributions and effects of temperature, effective strain rate, mean strain rate and mean stress are studied.