• Korean journal of applied entomology
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• v.34 no.4
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• pp.321-327
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• 1995

This study was conducted to determine the winter ecology of the small brown planthopper (SBPH), laodelphax striatellus Fallen, in Milyang, east Kyungsangnamdo province. The age distribution in the overwintering SBPH population varied according to collection dates. In early December, the population distribution was 60% 4th instar, 30% 3rd instar, 6% 5th instar, 3.4% 2nd instar with very few adults and 1st instar. In early March 5th instars had the highest propotion (47-50%) with 4th instar 44-46%. In early April the adult population was 75-81% of the population. The averaged nymphal instar converted the age distribution of he overwintering SBPH in to the into the numerical values tended to increase continuously. However, there were some differences in the averaged nymphal instar of overwintering SBPH annually and these differences resulted from different age distribution of the overwintering SBPH due to different annual temperature fluctuations during overwitering periods. The weight of the overwintering SBPH increased continuously during overwintering periods. The collection density of the overwintering SBPH population was significantly higher on the levee than in the barley field. Percent nymphal parasitism by haplogonatopus atratus in the overwintering SBPH population averaged about 21% regardless of overwintering years.

• Korean journal of applied entomology
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• v.35 no.2
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• pp.140-145
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• 1996

This study was conducted to investigate the winter ecology of the green leafhopper(GLH), Nephotettix cincticeps Uhler, in Milyang, south-eastern part of Korea from early December to early April during 1990 to 1992. The instar distribution in the overwinteing GLH population collected in December 5th was most of 4th instar 77% to 79% followed by 3rd instar 15% to 17% and 5th instar 3%. These instar distribution rate in the overwintering GLH was changed with collecting dates during overwintering periods of the GLH. Among the GLH population collected, as a result, the distribution rate of the 5th instar was significantly increased in February 20th and was most of 5th instar 70% to 71% in March 19th and was adult 47% to 50% in April 8th. During two overwintering periods of the GLH, the averaged instars converted the instar distribution of the overwintering GLH into the numerical values were 3.91 and 3.86 instar in December 5th, 4.11 and 4.07 instar in February 20th, 4.75 and 4.79 instar in March 19th and 5.42 and 5.45 instar in April 8th respectively. In addition to, the developmental index between pre-collected date and post-collected date of the overwintering GLH was roughly more than 1.0 value. It means that the overwintering GLH was developed without cease though the development was very slow and deponded upon air temperature. The overwintering GLH population were significantly more collected at the levee than at the barley field.

• The Journal of the Acoustical Society of Korea
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• v.16 no.2
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• pp.71-79
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• 1997

In this paper, a codeword-dependent distance normalization(CDDN) and an instar-formed fuzzy smoothing of output distribution are proposed for robust estimation of output probabilities in the FVQ(fuzzy vector quantization)-DHMM(discrete hidden Markov model). The FVQ-DHMM is a variant of DHMM in which the state output probability is estimated by the sum oft he product of the output probability and its weighting factor for each codeword on an input vector. As the performance of the FVQ-DHMM is influenced by weighting factor and output distribution from a state, it is required to get a method to get robust estimation of weighting factors and output distribution for each state. From experimental results, the proposed CDDN method has reduced 24% of error rate over the conventional FVQ-DHMM, and also reduced 79% of error rate when the smoothing of output distribution is also applied to the computation of an output probability. These results indicate that the use of CDDN and the fuzzy smoothing of output distribution to the FVQ-DHMM lead to improved recognition, and therefore it may be used as an alternative to the robust estimation of output probabilities for HMMs.

• Korean Journal of Ecology and Environment
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• v.38 no.spc
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• pp.27-30
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• 2005

Diel vertical migration (DVM) and horizontal distribution of Chaoborus flavicans larvae were studied in Lake Fukami-ike (maximum depth 7.75 m, Central Japan). The larvae showed a clear migration in the 4 ${\sim}$ 7 m layer during the day and throughout the water column at night. It should be noted that, while old instar distributed throughout the column during the night. Young instar was found only in the 1 ${\sim}$ 4 m layer. An examination of horizontal distribution revealed that larval stage was clearly related with the depth. It suggests that Chaoborus appear to exist in the deeper area during the larval stage.

• Korean journal of applied entomology
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• v.32 no.2
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• pp.134-138
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• 1993

Larval densities and spatial distribution patterns of beet armyWorm, Spodoptera exigua, were studied in the welsh onion field located in Asan from June to November, 1991. During the period, there were two denslty-peaks;mid August and mid~late September. The larvae showed clumped distribution patterns. but the patterns changed into random as larval density decreased in Oct.ober. Each larval instar showed clumped pattern expect 6th instar surveyed on September 25, which distributed in a random pattern. The larval distribution pattern were also influenced by the host plants;clumped pattern on such host. as red pepper and welsh onion, versus random pattern on such hosts as chrysanthemum, peanut and soybean.

• Journal of Sericultural and Entomological Science
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• v.38 no.2
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• pp.180-185
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• 1996

Biosynthesis tracing of the silk fibroin in Bombyx mori silkworm was examined in vivo with isotopic [1-13C] Gly. labeling by nuclear magnetic resonance method. The [1-13C] Gly. labeled silk fibroin yielded very sharp 13C NMR signal in the posterior silk gland as well as in aqueous solution and the amound of [1-13C] Gly. labeled signal in the silkworm increased gradually and rapidly to 5-th day of fifth instar. However, the decomposition or decrease of the [1-13C] Gly. labeled signal occured from 5-th to 9-th day of fifth instar unexpectedly. These findings suggest that a relative amount of ${\alpha}$-helical portion or amorphous silk II portion was formed without any further signal from 6-th day of fifth instar to pupation. Through peak separation of orientation spectrum, between the fiber axis and the molecular bond direction, N-H bond in Bombyx mori silk fiber as well as the orientation distribution around the silk fibroin axis were determined and two kinds of peaks were also obtained from this orientation spectrum.

• Korean journal of applied entomology
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• v.48 no.2
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• pp.165-170
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• 2009

This study was conducted to investigate specific stages and distribution of overwintering nymphs of green rice leafhopper, Nephotettix cincticeps by measuring head capsule width. The nymphal head capsule width of the 1st instar to the 5th instar was 0.381, 0.502, 0.673, 0.979 and 1.128 mm, respectively. lts coefficient variation was 5.3, 4.0, 3.0, 4.5 and 5.3%, respectively. Growth ratio of each instar was not significantly different among 2nd to 4th instars as 1.31 to 1.34, but for the 5th instar it decreased as 1.28. The logarithm of the nymphal head capsule width was regressed as a function of the stage number of insects, resulting in LogY = 1.4627 + 0.1192X ($r^2= 0.9993$). Also fitness to the Dyar's law for the nymphal head capsule width of each instar was 98% or over and the Dyar's constant (K) was 1.316. The occurrence of N. cincticeps was maximum at the end of January with 195 individuals/0.25 $m^2$. The most abundant instar in the overwintering N. cincticeps population was 4th instar accounting for over 90%. Population of the 5th instar began to increase from mid-March, and adults began to occur in early April.

• Animal cells and systems
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• v.1 no.1
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• pp.107-113
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• 1997

Antisera against the myotropic neuropeptide leucokinin I, originally isolated from head extracts of the cockroach Leucophaea maderae, have been used to investigate the distribution of the leucokinin I-immunoreactive (LK I-IR) neurons in the brain of the common cutworm, Spodoptera Iitura, during postembryonic development. The LK I-IR neurons are found at the larval stages (excluding first instar larval stage), pupal stages, and adult stage, of which the brains have been examined in this experiment. The number of the LK I-IR neurons in the brain increases from the second instar larva to the fifth instar larva which has about 32, the largest number in all postembryonic stages. Thereafter, the LK I-IR neurons begin to decrease in number. During the pupal stages, smaller number of LK I-IR neurons persist in the brains; 6 or 4. At adult stage the brain contains 8 LK I-IR neurons. The LK I-IR cell bodies are distributed in each dorsal cortex of both cerebral hemispheres in the second instar larva and through all the neuromeres of the brain during later larval stages, despite of being a large number of the LK I-IR cell bodies in dorsolateral neuromeres. At pupal stages, most of the LK I-IR cell bodies are found in the pars intercerebralis. Extremely small number of the LK I-IR cell bodies are localized in the pars lateral is. Adult brain contains the LK I-IR cell bodies in the pars intercerebralis and the middle cortex of the posterior brain. The LK I-IR nerve processes can be easily found in the neuropils of almost all the neuromeres in the brains of third, fourth, fifth and sixth instar larvae. Most of the LK I-IR nerve fibers in those brains are originated from the LK I-IR cell bodies located in the brains. The LK I-IR cell bodies which have very weak reactivities to the antisera do not show projection of the LK I-IR nerve processes in the brains.

• The Korean Journal of Zoology
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• v.25 no.2
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• pp.63-70
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• 1982

The activity, properties, and distribution of midgut protease during metamorphosis in Pieris rapae L. are determined using spectrophotometer, ultracentrifuge and agar gel electrophoresis. Proteolytic activity of midgut reaches the peak just before ecdysis in 5th instar and prepupal stages each but 1 day after ecdysis in pupal stage. Also, optimum pH of midgut protease is pH 8.0 in 5th instar stage, pH 6.5 in prepupal stage, and pH 8.5 immediately before emergence respectively. Protease is found mostly in midght tissue in 5th instar stage but thereafter until just before emergence the enzyme only in lumen contents, suggesting that protease is synthesized in midgut tissue during larval stage and then released into lumen during pupation period.

• The Korean Journal of Zoology
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• v.29 no.1
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• pp.1-12
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• 1986

Electrophoretic, immunological, and column chromatography methods were used to determine the appearance and distribution of storage proteins in various organs during the metamorphosis of Bombyx mori L. Two storage proteins start to appear in haemolymph in early 5th instar stage and show the identical mobility with fat body proteins. These proteins show the high concentration in haemolymph in last instar stage but accumulate in fat body after pupation. Storage protein-2 shows the distinct pattern for general storage proteins in both male and females. This protein is involved with the formation of cuticle protein in late last instar stage and appears to be temperally deposited in midgut during the pupal stage. Also SP-2 shows the identity with vitellogenin electrophoretically and immunologically and especially the positive reaction with antibody against yolk protein during the pupal stage, demonstrating that the storage protein is closely related to the formation of yolk protein.