• Title/Summary/Keyword: insoluble components

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Effect of Seatangle and Seamustard Intakes on Carcinogen Induced DNA Adduct Formation and the Absorption of Calcium and Iron (다시마와 미역의 섭취가 발암물질에 의한 DNA 손상과 칼슘 및 철 흡수에 미치는 영향)

  • 성미경
    • Journal of Nutrition and Health
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    • v.33 no.7
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    • pp.717-724
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    • 2000
  • A number of epidemiological studies has indicated lifestyles including dietary habits are closely related to the development of certain forms of cancer. These findings have led several investigators to identify the ways in which these factors mdulate the risk of cancer. Seaweeds are rich sources of non-digestible polysaccharides which possibly posses physiological functions. In vitro studies showed several components in seaweeds inhibit tumor cell growth and mutagenicity of known food mutagens. On the other hand non-digestible polysaccharides of different food sources negatively affect mineral nutrition by decreasing mineral absorption. The objectives of this study was to investigate the effect of major seaweed intake on azoxymethane(AOM) - induced DNA damage a known cancer initiation step and on apparent absorption of calcium and iron. To accomplish these objectives twenty five ICR mice were divided into five groups and fed one of the following diets for 10 days : control diet d, diet containing 10% water-soluble fraction of seamustard or seatangle diet containing 10% water-insoluble fraction of seamustard or seatangle. AOM was injected 6 hours before sacrifice and N7-methylated guanines from the colonic DNA were quantified using a gas chromatography -mass spectroscopy. Fecal samples were collected on days 4 and 8. Caclium and iron contents of the diets and feces were analyzed using an atomic absorption spectrophotometry to determine the apparent absorption of these minerals. Results are as follows. AOM-induced guanine methylation of colon was decreased in animals fed diets containing water-soluble fractions of seamustard or seatangle compared to those in animals fed control diet although only the seatnagle fed group showed statistically significant effect. Apparent calcium absorption was significantly reduced in animals fed diets containing water-insoluble fractions of seaweeds. Iron absorption was significantly decreased and negatively balanced in animals fed diets containing water-insoluble fractions of both seaweeds, and water-soluble fraction of seatangle. In conclusion, seamustard and seatangle intakes may effectively prevent colon tumorigenesis by reducing a carcinogen-induced DNA damages, and more mechanistic studies on possible role of seaweeds on carcinogenesis are required. Also, adverse effects of seaweed diets cintaming a large amount of polysaccharides on mineral nutrition should be carefully monitored.

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Characteristics of Total Atmospheric Deposition by the Filtration-Sampling Method at Coal-Fired Power Plant Area (여과식 채취방법에 의한 대기오염 총침착물의 특성 -석탄화력발전소 주변지역을 중심으로-)

  • 박정호;조인철;최금찬
    • Journal of Korean Society for Atmospheric Environment
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    • v.18 no.2
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    • pp.161-170
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    • 2002
  • Total(=wet+dry) atmospheric depositions were collected by filtration-sampling method at 17 sampling sites of the coal-fired power plant area from September 1999 to January 2000. The soluble and insoluble fractions of deposition were also measured to investigate a suitability of simplified collection method for a long-term monitoring of total deposition. In the study, the 50% of sampled soluble fractions showed the electric conductivity (E.C.) of below 50 $\mu$S/cm and the 42% of them showed the lower pH than 5.0. The monthly mean fluxes of water soluble ionic components; S $O_4$$^{2-}$, C $l^{[-10]}$ , N $O_3$$^{[-10]}$ , N $a^{+}$, N $H_4$$^{+}$, $K^{+}$, $Mg^{2+}$, $Ca^{2+}$ were 168.4 kg/k $m^2$.month, 100.5 kg/k $m^2$.month, 88.6kg/k $m^2$.month, 31.3kg/k $m^2$.month, 25.6 kg/k $m^2$.month, 13.3 kg/k $m^2$.month, 8.7 kg/k $m^2$.month, 43.1kg/k $m^2$.month, respectively. The mean ionic concentration of all sample(n=79) was 314 $\mu$eq/ι, with contributions of 24.2% and 23.0% by [nss-C $a^{2+}$] and [nss-S $O_4$$^{2-}$]. The ratio of [N $O_3$$^{[-10]}$ ]/[nss-S $O_4$$^{2-}$] and [N $H_4$-C $a^{2+}$] were found to be 0.52 and 0.68, respectively.espectively.

A physiological study on Sporulation of Rhizopus nigricans (Rhizopus nigricans의 포자형성에 관한 생물학적 연구)

  • 윤경하;이영록
    • Korean Journal of Microbiology
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    • v.17 no.2
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    • pp.81-93
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    • 1979
  • The mycelium of Rhizopus nigricans was harvested at intervals during the sporulation periods, fractioned into various cell components and analyzed the con!eiits of various cell materials in order to clarify the optimum conditions of sporulation and some characteristics of the metabolism during tke sporulation periods. The changes in enzyme activities, such as amylase and protease, were also measured during the sporulation period,. 1. Mycelium in distilled water culture, as control, did not sporulate but mycelial mat cultured in Petridish without mutrient spourulated. Optimum temperature range for sporulation was $20{\sim}25^{\circ}C$. 2. During the sporulation and maturation periods, proteins, especially alkali-labile protein were decreased remarkably but free amino acid and ninhydrin reactive substances in acid soluble fraction were increased, compared with control. 3. Acid solable polyphosphate was decreased but acid insoluble polyphosphate was increased, during the sporulation. 4. Carbohydrate and hexosamine in acid soluble fraction were increased, while carbohydrate in alkali insoluble residual fraction was decreased during the sporulation periods. 5. Amounts of UV-absorbing material in deoxyribonucleic acid fraction was increased a little but those in ribonucleic acid fraction was decreased, compared with control. 6. Intracellular amylases and proteases activities insporulating mycelial mat were increased continuously during the sporulation and maturation periods.

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Effect of dextranase and glucose-oxidase on the formation of plaque by Streptococcus mutans (Streptococcus mutans의 Plaque 형성에 미치는 Dextranase와 Glucose-oxidase 의 영향)

  • 김윤석;안재현;정광례;이기붕
    • Korean Journal of Microbiology
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    • v.27 no.4
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    • pp.430-435
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    • 1989
  • Dextranase and glucose-osidase was investigated as an anti-plaque agent and a component of dentifrice. In vitro synthesis of the water-insoluble glucan was decreased with increasing amount of dextranase and glucose-oxidase. Dextranase was effective on the decrease of viable S. mutans, and the formation of plaque decreased. But it is not effective on the degradatio of plaque. As a research for addition of enzyme to the dentifrice components, we formulated the Model Dentifrice for stabilization of enzyme. At the Model Dentifrice, we confirmed the stability of enzyme by evalution of activity for a long time.

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Properties of $\beta$-1,3-glucan Synthase System in Saccharomyces cerevisiae (Saccharomyces cerevisiae의 베타-1,3-글루칸 합성효소 체계의 특성)

  • Park, Hee-Moon;Kim, Jeong-Yoon;Kim, Sung-Uk;Bok, Song-Hae
    • Microbiology and Biotechnology Letters
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    • v.23 no.3
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    • pp.316-321
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    • 1995
  • Some properties of $\beta$-1, 3-glucan synthase system in Saccharamyces cerevisiae were investigated. By extraction with detergent and salt, the membrane preparations could be dissociated into two components, one soluble, the other still membrane bound. Both components, in addition to GTP, were necessary for the activity of $\beta$-1, 3-glucan synthase like other fungi. The protective effect of guanosine nucleotides on the soluble factor pointed to the possibility that this fraction contained a GTP-binding protein. Addition of increasing amounts of soluble factor to a constant amount of insoluble catalytic factor, vice versa, gave rise to a saturation curve. These results, including different types of evidence, indicate that the soluble factor and the catalytic factor form a complex.

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Comparison of Quality Characteristics of Soybean Curd and Whole Soybean Curd (전두부와 일반두부의 품질 특성 비교)

  • Lee, Hyoung-Jae;Shin, Heung-Chule;Lee, You-Seok;Kim, Jin-Young;Moon, Young-Hak;Park, Keun-Hyung;Moon, Jae-Hak
    • Korean Journal of Food Science and Technology
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    • v.41 no.2
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    • pp.117-121
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    • 2009
  • Soybean curd (SC) is prepared through the elimination of water-insoluble components and molding by pressing. However, whole soybean curd (WSC) is made without the elimination of water-insoluble components and pressing. Therefore, it was considered that the components contained in WSC might be different from those in SC and we compared their contents of various components, hardness, and morphological features for this study. Ash, dietary fiber, and niacin contents were significantly higher in WSC than in SC. In addition, the carbohydrate and vitamin $K_1$ contents of WSC tended to be higher than those in SC, whereas the hardness of WSC was lower than that of SC. Moreover, electron microscopic analysis showed that the morphological characteristic of WSC consisted of considerably more fine retiform tissues in comparison to SC. Based on their compared nutrition, functional, and quality characteristics, it was suggested that WSC might be superior to SC.

A Study of the Chemical Components of the Halophyte Suaeda asparagoides $M_{IQ}$ (염생식물 나문재(Suaeda asparagoides $M_{IQ}$)의 이화학적 성분 조성)

  • Lee, Young-Keun;Lee, Yoon-Shin;Jung, Eun-Kyung
    • Journal of the East Asian Society of Dietary Life
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    • v.20 no.3
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    • pp.452-457
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    • 2010
  • To obtain basic information on the utilization of Suaeda (S.) asparagoides $M_{IQ}$ as a raw material in food, it's chemical components of were investigated. S. asparagoides $M_{IQ}$ from the Hwaseong-si in Korea was used after drying and grinding with powder. The total dietary fiber, insoluble dietary fiber and soluble dietary fiber contents of S. asparagoides $M_{IQ}$ were found to be 39.41%, 37.63% and 1.78%, respectively. Macromineral components of S. asparagoides $M_{IQ}$ were Na (7.6%), K (2.4%) and Mg (0.6%). High contents of microminerals such as Cu, Mn and Zn were also found. Lysine and methionine which are essential amino acids were detected with the value of 215 mg/100 g and 23.2 mg/100 g, respectively. S. asparagoides $M_{IQ}$ that was harvested in May was higher in crude protein than that harvested in September but the crude fat content of that which was harvested in September was twice as high as that harvested in May.

Studies on the Softening of Strawberry during Circulation and Storage (1) Changes of Cell Wall Components, Protein and Enzymes during Ripening (딸기의 유통.저장시 연화현상에 관한 연구 (1) 세포벽 성분, 단백질 및 효소의 변화)

  • 이광희;김광수;김미현;신승렬;윤경영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.1
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    • pp.29-34
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    • 1998
  • This study was investigated to know changes of the cell wall components, cell wall degrading enzyme activities and contents of soluble protein of strawberry during ripening and softening. The contents of water soluble substances were slightly increased during ripening, but the contents of alcohol-insoluble substances were not changed. The contents of pectin were not changed at green mature and turning stage, while decreased after mature stage. The contents of alkali-soluble hemicellulose and cellulose were increased during ripening and softening. The contents of water-soluble and saltsoluble protein were not changed, but the content of cell wall protein was slightly decreased during ripening. The content of total protein was increased at turning stage, it is not changed after turning stage. $\beta$-Galactosidase activity was increased during ripening, and pectinmethylesterase activity was decreased at turning. Phenylalanine ammonia-lyase activity was changed up to mature stage, but decreased at overripening stage. Polygalacturonase and cellulase activities were not detected at all of ripening stages.

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Changes of Sugar Components in Cell Wall Polysaccharides from Tomato Fruits during Ripening (토마토 과실의 성숙중 세포벽 구성다당류의 변화)

  • Mun, Gwang-Deok;Cheon, Seong-Ho;Kim, Jong-Guk
    • Food Science and Preservation
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    • v.3 no.2
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    • pp.113-120
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    • 1996
  • This study was conducted to understand the characteristics of fruit softening during ripening which causes deep loses in quality of horticultural products during storage and marketing process after harvest. The changes of cell wall components during ripening was investigated. The climacteric rise was between 42 and 49 days after anthesis and then decreased. Ethylene evolution was similar to respiration. The hardness of fruit decreased markedly at this climacteric period and significances of textural parameters among the ripening periods were recognized but the significance between 50 and 55 days after anthesis was not. Sugar components of cell wall polysaccharides were uronic acid, galactose, glucose, arabinose, xylose, rhamnose, mannose and fucose. The contents of arabinose and mannose in alcohol-insoluble solids fraction increased, but other sugars were not changed. In cell wall fraction, the contents of uronic acid, galactose, glucose and arabinose were comparatively high, but galactose, arabinose and ironic acid were decreased markedly during ripening. ironic acid occupied above 75% of total monosaccharide in pectin fraction and decreased markedly during ripening. In acid-soluble hemicellulose fraction, the contents of uronic acid, glucose, galactose and rhamnose were high and they decreased from 50 days after anthesis. The contents of glucose and xylose were high in a alkali-soluble hemicellulose fraction and they decreased markedly at 55days after anthesis.

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Estimations of the Optical Properties and Direct Radiative Forcing of Aerosol Chemical Components in PM2.5 Measured at Aewol Intensive Air Monitoring Site on Jeju Island (제주 애월 대기오염집중측정소의 PM2.5 에어로졸 화학성분 자료를 이용한 광학특성 및 직접적 복사강제력 추정 연구)

  • Park, Yeon-Hee;Song, Sang-Keun;Kang, Chang-Hee;Song, Jung-Min
    • Journal of Korean Society for Atmospheric Environment
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    • v.33 no.5
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    • pp.458-472
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    • 2017
  • The optical properties and direct aerosol radiative forcing (DARF) of different aerosol components in $PM_{2.5}$ (water-soluble, insoluble, black carbon (BC), and sea-salt) were estimated using the hourly resolution data measured at Aewol intensive air monitoring site on Jeju Island during 2013, based on a modeling approach. In general, the water-soluble component was predominant over all other components with respect to its impact on the optical properties(except for absorbing BC) and DARF. The annual mean aerosol optical depth (AOD) at 500 nm for the water-soluble component was $0.14{\pm}0.14$ ($0.04{\pm}0.01$ for BC). The total DARF at the surface ($DARF_{SFC}$) and top of the atmosphere ($DARF_{TOA}$), and in the atmosphere ($DARF_{ATM}$) for most aerosol components(except for sea-salt) during the daytime were highest in spring and lowest in fall and/or summer. The maximum $DARF_{SFC}$ of most aerosol components occurred around noon (12:00~14:00 LST) during all seasons, while the maximum $DARF_{TOA}$ occurred in the afternoon (13:00~16:00 LST) during most seasons (except for spring). In addition, the estimated $DARF_{SFC}$ and $DARF_{ATM}$ of the water-soluble component were -20 to $-59W/m^2$ and +3.5 to $+14W/m^2$, respectively, while those of BC were -18 to $-29W/m^2$ and +23 to $+37W/m^2$, respectively.