• 가축개량기술정보
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• no.10
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• pp.1-8
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• 1996

• 가축개량기술정보
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• no.13
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• pp.1-12
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• 1996

• 가축개량기술정보
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• no.14
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• pp.1-12
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• 1997

• 가축개량기술정보
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• no.22
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• pp.1-16
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• 2002

• 가축개량기술정보
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• no.16
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• pp.1-12
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• 1998

• 가축개량기술정보
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• no.24
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• pp.1-10
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• 2003

• 가축개량기술정보
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• no.7
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• pp.1-12
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• 1994

• 한국가축인공수정사협회:학술대회논문집
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• v.2004 no.05
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• pp.27-31
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• 2004

• 가축개량기술정보
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• no.23
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• pp.1-10
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• 2002

• Clinical and Experimental Reproductive Medicine
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• v.43 no.3
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• pp.157-163
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• 2016

Objective: The decision to use in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), or split insemination (IVF-ICSI) in the first cycle is based on the number of motile sperm. Hence, total fertilization failure (TFF) often occurs during IVF cycles, despite normozoospermia. To investigate whether the cumulative motile swim-up spermatozoa percentage at 22 hours post-insemination (MSPPI) is an indicator for ICSI, we analyzed TFF, fertilization, blastocyst development, chemical pregnancy, clinical pregnancy, and live birth rates. Methods: This prospective study was performed using data obtained from 260 IVF cycles. At 22 hours after insemination, the remaining swim-up spermatozoa were observed and divided into six groups according to MSPPI (<10%, 10% to <30%, 30% to <50%, 50% to <70%, 70% to <90%, and 90% to 100%). Results: Regardless of the ejaculated motile sperm concentration ($0.6-280{\times}10^6/mL$ motile spermatozoa), the incidence of TFF significantly increased when MSPPI was <10%, and the fertilization rate significantly decreased when MSPPI was <30%. We found that cumulative MSPPI correlated with the cumulative fertilization rate (Spearman correlation, 0.508, p<0.001). Regarding embryo development, we observed no significant differences in the rates of blastocyst development, chemical pregnancy, clinical pregnancy, or live birth among all groups. Conclusion: Our findings suggest that MSPPI is a viable indicator for split IVF-ICSI and ICSI. Taken together, by employing the MSPPI test in advance before IVF, ICSI, or split IVF-ICSI cycles, unnecessary split IVF-ICSI and ICSI may be avoided.