• Journal of Life Science
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• v.21 no.8
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• pp.1168-1175
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• 2011

This study was carried out to offer forensic entomological evidence to estimate the post mortem interval (PMI) based on the arthropod succession in pig carrions and carrion decomposition patterns varying with the exposed extent of the carrions. Four carrions, exposed, wrapped, covered or shaded, were placed in an open grassland near the Nakdong-river in Busan in August, 2010. It was found that the first visiting insect was Chrysomyia megacephala among a total of 20 species of 15 families belonging to 7 orders of Insecta, and that four species were useful indicators of the decomposition stage of the carrion: C. megacephala for I~V stages; a species of Sepsidae, Creophilus maxillosus and a species of Carabidae for III~V stages. It was also found that the more the exposed extent of exposure of carrions, the higher the rate of decomposition of carrions, i. e. the time for decomposition of carrions were 7days for the exposed carrion, 10days for the covered one, 13days for the shaded one, and 15days for the wrapped one. This result seems to be related to the differences in the weight loss of carrions caused by strong rains during the research period and by the accessibility of insects to the carrions; both are strongly affected by the exposed extent of the carrions. Accordingly, it was suggested that the exposed extent of the carrions must be taken into consideration to estimate the PMI.

• Journal of Sericultural and Entomological Science
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• v.41 no.1
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• pp.36-40
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• 1999

The optimal condition for the production of the artificial fruiting bodies of Paecilomyces japonica in bulk was determined from the observation of the conidial, hyphal, and cultural condition and the medium. The colony was grown 32 mm diameter in 14 days on potato dextrose agar(PDA). Conidia was irregularly long oval-shaped and measured 4.07${\times}$1.56 ${\mu}$m in average. The hyaline hyphae formed transparent bundles which branched out. The fruiting body on the insect surface was measured 30 to 50 mm in length and formed up to 55 branches. Potato dextrose(PD) both was the most effective for the growth of Paecilomyces japonica among the liquid type media. About 3.1 mg mrcelia in dry weight were produced in 50 ml/PD broth. The best condition for the production of conidia under which condition 4.3${\times}$10$^8$ conidia/ml were harvested was pH 5.0 in acidity and 20$^{\circ}C$ following preculture at 24$^{\circ}C$ in temperature for 7 days.

• Parasites, Hosts and Diseases
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• v.52 no.1
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• pp.93-97
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• 2014

Subolesin (4D8), the ortholog of insect akirins, is a highly conserved protective antigen and thus has the potential for development of a broad-spectrum vaccine against ticks and mosquitoes. To date, no protective antigens have been characterized nor tested as candidate vaccines against Dermacentor silvarum bites and transmission of associated pathogens. In this study, we cloned the open reading frame (ORF) of D. silvarum 4D8 cDNA (Ds4D8), which consisted of 498 bp encoding 165 amino acid residues. The results of sequence alignments and phylogenetic analysis demonstrated that D. silvarum 4D8 (Ds4D8) is highly conserved showing more than 81% identity of amino acid sequences with those of other hard ticks. Additionally, Ds4D8 containing restriction sites was ligated into the pET-32(a+) expression vector and the recombinant plasmid was transformed into Escherichia coli rosetta. The recombinant Ds4D8 (rDs4D8) was induced by isopropyl ${\beta}$-D-thiogalactopyranoside (IPTG) and purified using Ni affinity chromatography. The SDS-PAGE results showed that the molecular weight of rDs4D8 was 40 kDa, which was consistent with the expected molecular mass considering 22 kDa histidine-tagged thioredoxin (TRX) protein from the expression vector. Western blot results showed that rabbit anti-D. silvarum serum recognized the expressed rDs4D8, suggesting an immune response against rDs4D8. These results provided the basis for developing a candidate vaccine against D. silvarum ticks and transmission of associated pathogens.

• Journal of Life Science
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• v.30 no.4
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• pp.386-393
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• 2020

Insect industry has been focused as production of food, animal feed, pollinator and for environmental remediation. Hermetia illicens, called as black soldier fly (BSF) is famous as nutritive feed. In this study, to evaluate the antithrombotic activities of BSF, the larvae (instar 2~6), pupae, residue after adult emergence [RAAE] and adult of Hermetia illicens [black soldier fly, BSF] were collected and their ethanol extracts were prepared. Growth of BSF larvae was very rapid and the weight of larvae was increased to 25-folds during 10 days cultivation. The ethanol extraction ratios showed from 1.0% (pupae) to 18.5%(adults). The highest total polyphenol, total flavonoid, and total sugar contents were observed in RAEE (17.2 mg/g), pupae (3.4 mg/g), and instar 6 (37.6 mg/g), suggesting that metabolic changes occur during the life cycle of the BSF. Anti-coagulation assay showed that extracts of RAEE, instar 6 and pupa of BSF significantly inhibited thrombin, prothrombin, and blood coagulation factors. Furthermore, the extracts of RAEE, instar 3 and adult of BSF showed a strong platelet aggregation inhibitory activity. Our results suggest that pupae and RAEE of BSF have potential as antithrombotic agents. This is the first study to provide evidence of the antithrombotic activity of the BSF and bioactivity alterations during its life cycle.

• Parasites, Hosts and Diseases
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• v.34 no.1
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• pp.35-48
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• 1996

Non-biting midges fchironomidae, Dipteral are one of the largest insect families, which are distributed worldwidely and are found in nearly all types of inland waters. They are known to be aggressive inhalant allergens which cause allergenic diseases. In this study, the crude antigens of Chironomus SavipLumn adults which are most widely distributed in Korea were extracted. and their allergens were analysed with the sera from experimentally sensitized mice. The mice were immunized with $1{\;}\mu\textrm{g}{\;}or{\;}10{\;}\mu\textrm{g}$ of the crude antigens, respectively, and the specific serum IgE levels were measured by both ELISA and passive cutaneous anaphylaxis (PCA) techniques. The highest levels of both total IgE and chironomid-specific IgE were found in the mouse sera obtained after 9 weeks of the first infection with $1{\;}\mu\textrm{g}$ crude antigen. The crude antigen was separated into 16-18 protein bands on gel by SDS-PAGE. The crude extract was assessed by SDS-PAGE/immunoblot analysis. One IgE-binding band (65 kDa) was detected by developing with colorimetric substrate, and 4 IgE-binding bands (65, 52, 35 and 25 kDa) by developing with CSPD chemiluminescent substrate. The SDS-PAGE gel of the crude extract of chironomid adults was equally cut into 30 pieces and each of them was eluted to isolate proteins by molecular weight, and the allergenicity of each eluate was assessed by applying P-K test on rats. Proteins of 65, 35 and 15 KDa showed the highest P-K titer (${\times}512$) which was 16 times higher than that of the crude extract (${\times32}$). The P-K titer of 52 kDa protein was also 4 times higher ($128{\times}$) than that of the crude extract, whereas the 25 kDa protein poorly responded, which seemed not antigenic. In conclusion, the present result in mice demonstrated that adults of Chironomus fcuiplumus, a predominent species in Korea, cause allergenic diseases and the main allergens are 65, 52, 35 and 15 kDa proteins, of which 65 kDa protein seems to be a main allergen.

• Food Science and Industry
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• v.53 no.1
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• pp.69-83
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• 2020

For the development of the chitosan industry in Korea, the catch of red snow crabs caught on the east coast is rapidly decreasing. Therefore, it is urgent to develop raw materials that can replace the red snow crab as the top priority to solve the supply and demand problems, as well as wastewater treatment costs account for a large proportion of the cost of chitosan. In order to solve the problems, continuous research on biological extraction methods such as enzymatic extraction and microbial fermentation will increase production efficiency and lower unit cost as well as chemical extraction methods. Further efficient manufacturing method can be established. Establishing of novel techniques is indispensable for production of high-purity chitosan and the ability to regulate and separate the molecular weight, as well as joint research with industry, academia and research institute for the research and development of high-functional chitosan derivatives.

• Journal of Animal Science and Technology
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• v.64 no.6
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• pp.1063-1076
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• 2022

Two experiments were conducted to determine the effect of Hermetia illucens larvae (HIL) as protein and protease on growth performance, blood profiles, fecal microflora, and gas emission in growing pig. In experiment 1, the seventy-two crossbred growing pigs ([Landrace × Yorkshire] × Duroc) with an initial body weight (BW) of 27.98 ± 2.95 kg were randomly allotted to one of four dietary treatments (3 pigs per pen and 6 replicates pen per treatments). The experimental design was a 2 × 2 factorial arrangement of treatments evaluating two diets (Poultry offal diets and HIL diets) without or with supplementing protease. The poultry offal in basal diet has been replaced by HIL. In experiment 2, the four crossbred growing pigs ([Landrace × Yorkshire] × Duroc) with an initial BW of 28.2 ± 0.1 kg were individually accepted in stainless steel metabolism cages. The dietary treatments included: 1) PO- (PO-; poultry offal diet), 2) PO+ (PO- + 0.05% protease), 3) HIL- (3% PO of PO- diet was replacement 3% HIL), 4) HIL+ (HIL- + 0.05% protease). In experiment 1, From weeks 0 to 2, average daily gain (ADG) and feed efficiency (G:F) were significantly increased in the PO diet group compared with the HIL group. From weeks 2 to 4, ADG and G:F were higher for protease group than for non-protease group. At weeks 2 and 4, the PO diet group had lower blood urea nitrogen (BUN) levels than HIL diet group. In experiment 2, crude protein (CP) and nitrogen (N) retention were decreased by HIL diet at weeks 2 and 4. The fecal microflora and gas emission were not affected by HIL and protease. The HIL diet showed lower CP digestibility than PO diet and total essential amino acids digestibility tended to higher in PO diet than HIL diet. In summary, the present study revealed that replacement of the PO protein with the HIL protein and the additive of protease in growing pig diets during the overall experimental period had no negative effect.

• Journal of Sericultural and Entomological Science
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• v.13 no.2
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• pp.123-133
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• 1971

The author is the first man who tried to use an anesthetic on insect specially for silkworm in orde to evaluate the silkworm health and silk yielding ability and the obtained results are as followings. 1. The necessary ether vapor induction for narcosis on silkworms is varied by the glowth of the silkworm which the larger worm is, the longer induction is required. For instance, it was 2∼3 minutes for the worms of third day fifth instar silkworm in case use of ether anesthetic. 2. The longer anesthetic induction for silkworms, the longer recovery needs from anesthesia. In case five minutes ether vapor induction, silkworms recovered narcosis during in 5∼130 minutes which had varied very much by the health variation. 3. The ether induction caused silkworm to vomit digestive juice from a few per cent of the worms, but the chloroform induction showed majority of the worms to vomit the digestive juice out of mouth. So, the ether was found as better anesthetic for silkworms. 4. When ether induction last more than 20∼30 minutes, the recovered silkworms can eat mulberry, but when it gets more than three hours they can not eat mulberry. And when it last more than ten minutes, the silkworm may eat mulberry leaf, but they can not spinn cocoon properly. 5. In case five minutes ether induction for silkworms on third day fifth instar, the stronger variety is, the rallier recovered from narcosis. 6. The recovering duration from narcosis varies regarding with each worm health which shows Poisson′s distribution even in a same variety silkworm. 7. The female worms recover from narcosis earlier than male worms which means the female worm is stronger than male one. 8. The later recovered silkworm from narcosis spinned more rich cocoon silk and ended with smaller pupae weight. Such a tendency showed until at some recovery duration, then the silk yield droped down on the worms recovered in more longer duration. The author (Choe) had named such a relation curve as "Silk Yield Curve against Silkworm Health." 9. The silk yield or cocoon layer ratio had varied from 13 to 27% even in a same worm varity cocoon which showed serious variation and call attention carefulness for the duplication work of a variety silkworm eggs. 10. Not always the rich silk yielding worm is the best worm during the silkworm selection and it should be considered with the silkworm health evaluation. 11. At present situation, only specific breeding expert is allowed to join in the selection service because of need many years experience by use of visual observation, but the ether anesthesia method may help such an evaluation with more accuracy and easy way even for the people in fresh on the field. 12. The effect of the narcosis on the silkworm for the next generation or hybrid worm will be reported in next publication.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.4
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• pp.435-441
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• 2017

The present study was carried out to evaluate the applicability of Tenebrio molitor larvae (mealworm) as a health functional food material in order to contribute to the development of the domestic insect industry and health functional food industry. Protein hydrolysates were prepared from mealworm powder by enzymatic hydrolysis using five different proteases (alcalase, bromelain, flavourzyme, neutrase, and papain), and the hydrolysates were then tested for their antioxidant activities. Based on available amino group contents and sodium dodecyl sulphate-polyacrylamide gel electrophoresis analyses, mealworms treated with alcalase ($4,781.39{\mu}g/mL$), flavourzyme ($5,429.35{\mu}g/mL$), or neutrase ($3,155.55{\mu}g/mL$) for 24 h showed high degree of hydrolysis (HD) value, whereas HD values of bromelain ($1,800{\mu}g/mL$) and papain-treated ($1,782.61{\mu}g/mL$) mealworms were much lower. Protein hydrolysates showing high HD values were further separated into > 3 kDa and ${\leq}3kDa$ fractions by a centrifugal filter system and then lyophilized, and the production yields of the low molecular weight protein hydrolysates (${\leq}3kDa$) by alcalase, flavourzyme, and neutrase were 42.05%, 26.27%, and 30.01%, respectively. According to the RC_{50} values of the protein hydrolysates (${\leq}3kDa$) obtained from three different antioxidant analyses, all three hydrolysates showed similar antioxidant activities. Thus, alcalase hydrolysates showing the highest production yield of low molecular weight protein hydrolysates were further tested for their inhibitory effects on peroxidation of linoleic acid by measuring thiobarbituric acid values, and the results show that peroxidation of untreated linoleic acid increased dramatically during 6 days of incubation. However, pretreatment with the hydrolysates ($100{\sim}800{\mu}g/mL$) significantly inhibited linoleic acid peroxidation in a dose-dependent manner over 6 days.

• Korean journal of applied entomology
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• v.12 no.3
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• pp.115-120
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• 1973

An experiment was carried out to investigate possible use of several systemic insecticides as seed treatment to control some insect pests of rice, barley and soybean. The insecticides were impregnated with active carbon dust and $2\%$ water solution of methyl cellulose as skicker. The seeds were dressed with the insecticides: Fussol (Monofluoroacetamide), Dimethoate (0, 0-dimethyl-S-(N-methyl carbamoylmethyl) phosphorodithioate), Metasystox (0, 0-dimethyl 0-2 ethylthioethyl phosphorodithioate), Dimecron (0, 0-dimethyl (diethylamide-1-chlorocrotonyl) Phosphate). The seeds were treated at the rate of $4\%$ by grain weight. Effects of seed treatments with systemic insecticides on the seed germination and insecticidal effectiveness were tested. Fer rice, no seed germination was observed in Fussol treatment. Dimethoate, Metasystox and Dimecron showed a slight reduction in germination when seeds were sown 1 day after treatment, but they showed gradual reduction in germination when seeds were sown 20 and 45 days after treatment. Especially Diriethoate treatment showed significant reduction in germination after 20 and 45 days of storage. All the insecticides reduced seedling stands of barley somewhat. No plant emergence was observed in Fussol treatments after 25 and :to days of storage. In case of the 40 days of storge in barley seed treated with Dimethoate, Hetasystox and Dimecron there was no gignificant reduction in plant emergence except Fussol treatment. Dimethoate was highly toxic to the soybean seed viability. However, there was no significant reduction in the soybean germination after 1 day of storage. in Fussol, Metasystox and Dimecron treatments After 25 days of storag in Fussol and Dimethoate treatments, no plant emergence was observed, and there was $13.3\%$ emergence in Metasystox treatment, and $93.0\%$ emergence in Dimecron treatment. Dimethoate treatment in rice seed(variety Suwon # 82) showed $90\%$ mortality at the twentieth day and $33.3\%$ at the thirtieth day after sowing against the green rice leafhopper, Nephotettix cincticeps But no visible effectiveness was observed in Metasystox and Dimecron treatments. Dimethoate treatment in barley seed resulted in low population density of aphids. Rhopalosiphum spp. Final observation on the number of eggs per plant was made at the fiftieth day after sowing; 2 in Dimethoate, 4 in Fussol, 6 in Metasystox, 7 in Dimecron, and 13 in check plots. All the insecticides tested resulted in good insecticidal effectiveness against the aphids, Aulocorthum solani on soybean plant at the ninth day infestation after sowing. Dimethote, Metasystox, and Dimecron treated soybean plants was still effective in controlling the aphids to the eighteenth day infestation.