• Title/Summary/Keyword: inner region

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Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China

  • Zhao, Ling;Zhang, Liang;Shi, Xiaona;Duan, Xujie;Li, Huiping;Liu, Shuying
    • Journal of Veterinary Science
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    • v.22 no.6
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    • pp.66.1-66.9
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    • 2021
  • Background: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. Objectives: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. Methods: Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis. Results: A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%-86.8% and 67.7%-75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5). Conclusions: The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.

The Diameter Expansion of 6H-SiC Single Crystals by the Modification of Inner Guide Tube (새로운 가이드 튜브를 통한 6H-SiC 단결정의 직경 확장에 관한 연구)

  • Son, Chang-Hyun;Choi, Jung-Woo;Lee, Gi-Sub;Hwang, Hyun-Hee;Choi, Jong-Mun;Ku, Kap-Ryeol;Lee, Won-Jae;Shin, Byoung-Chul
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.21 no.9
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    • pp.795-800
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    • 2008
  • A sublimation method using the SiC seed crystal and SiC powder as the source material is commonly adopted to grow SiC bulk single crystal. However, it has proved to be difficult to achieve the high quality crystal and the process reliability because SiC single crystal should be grown at very high temperature in closed system. The present research was focused to improve SiC crystal quality grown by PVT method through using the new inner guide tube. The new inner guide tube was designed to prevent the enlargement of polycrystalline region into single crystalline region and to enlarge the diameter of SiC single crystal. The 6H-SiC crystals were grown by conventional PVT process. The seed adhered on seed holder and the high purity SiC source materials are placed on opposite side in sealed graphite crucible surrounded by graphite insulation. The SiC bulk growth was conducted around 2300 $^{\circ}C$ of growth temperature and 50 mbar in an argon atmosphere of growth pressure. The axial thermal gradient across the SiC crystal during the growth was estimated in the range of 15${\sim}$20 $^{\circ}C$/cm.

Marginal and internal fit according to the shape of the abutment of a zirconia core manufactured by computer-aided design/computer-aided manufacturing (CAD/CAM으로 제작된 지르코니아 코어의 지대치 형태에 따른 변연 및 내면 적합도에 관한 연구)

  • Kim, Ji-Su;Ryu, Jae-Kyung
    • Journal of Korean Dental Hygiene Science
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    • v.5 no.1
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    • pp.13-19
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    • 2022
  • Background: In this study, zirconia copings were fabricated by setting clinically acceptable inner values for prostheses using computer-aided design/computer-aided manufacturing (CAD/CAM). The processed copings were evaluated for the marginal and internal fit of each abutment shape with a CAD program using the silicone replica technique. Methods A total of 20 copings was produced by selecting models commonly used in clinical practice. After injecting the sample, the minimum thickness, internal adhesion interval, and distance to the margin line were set to 0.5, 0.05, and 1.00 mm using a dental CAD program, respectively. It was measured using a 2D section function in a three-way program of the silicon replication technology. Although the positions and number of measurements of the anterior and posterior regions differed, nine parts of each pre-tube were designated and measured by referring to a previous study to compare the two samples. Results As a result, the average margin of the mesial, distal, and buccal (labial) surfaces was 59.90 ㎛ in the anterior region and 60.40 ㎛ in the posterior region. The mean axial wall margin was 67.25 ㎛ in the anterior region and 69.25 ㎛ in the posterior region. In occlusion, the anterior teeth (77.70 ㎛), posterior teeth (77.60 ㎛), and both anterior and posterior regions were within the clinically acceptable range. Conclusion The edge and inner fit of zirconia coping manufactured using the CAD/CAM system showed clinically applicable results. To reduce errors and increase accuracy, materials and machine errors that affect the manufacture of prosthetics should be investigated. Based on our results, the completeness of prosthetics could increase if the inner value and characteristics of the material are adjusted when applied in clinical practice.

Application to the Flow in HDD Using PIV Measurement (HDD내 유동장에 대한 PIV 계측 기술의 적용)

  • 공대위;주원구;도덕희
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2004.05a
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    • pp.742-747
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    • 2004
  • Hard disk drives (HDD) in computer are used extensively as data storage capacity. PIV measurement system was used fur 2-dimensional visualization of the unsteady flow between co-rotating disks in air both with a shroud and both with a actual-like case. Geometric parameters are gap height (H) between disks in the case of shroud. The lobe- structured boundary between inner region and outer region was visualized, and the number of dominant vertices was determined clearly. It is found from flow visualization that the number of vortex cells can be correlated with Reynolds number based on H which is defined as R $e_{H}$ =ΩRH/v ranging from 7.96$\times$10$^2$ to 1.43$\times$10$^4$, and decreases as the disk speed increases. In the case of a actual-like case, the boundary between inner region and outer region appears cleary when head position located at outer diameter with no damper. It is detected with a case of head position middle diameter that the tip wake is generated behind HGA using PIV measurement and calculation.n.

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X-ray Image Processing for the Korea Red Ginseng Inner Hole Detection (II) - Results of inner hole detection - (홍삼 내공검출을 위한 X-선 영상처리기술 (II) - 내공검출결과 -)

  • 손재룡;최규홍;이강진;최동수;김기영
    • Journal of Biosystems Engineering
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    • v.28 no.1
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    • pp.45-52
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    • 2003
  • Red ginsengs are inspected manually by examining those in the dark room with back light illumination. Manual inspection is often influenced by physical condition of inspectors. Sometimes. the best grade, heaven. has some inner holes though it was inspected by a specialist. In order to resolve this problem, this study was performed to develop image processing algorithm to detect the inner holes in the x-ray image of ginseng. Because of little gray value difference between background and ginseng in the image. simple thresholding method was not appropriate. Modified watershed algorithm was used to differentiate the inner holes from background and normal ginseng body. Inner hole edge region detected by watershed algorithm consists of many number of blobs including normal portions. With line profile analysis with scanning one line at a time beginning the starting point. it shelved two peaks both ends representing extracting each blobs. in which setting threshold value as of lower peak value enabled us to obtain inner hole image. Once this procedure has to be done till the finishing point it is completing inner hole detection for one blob. Thus. conducting ail blobs by this procedure is completing inner detection of one whole ginseng. Detection results of the inner holes fer various size of red ginsengs were good even though there was small detection variation. 6.2%. according to position of x-rat tube.

Simulation of Mixing Transport on Inner Reservoir and Influence Impacts on Outer Region for the Saemankeum Effluents Caused by Gate Operation (새만금호 수문 개방에 따른 내측의 혼합수송 및 외해역의 방류영향모의)

  • Suh Seung-Won;Cho Wan-Hei;Yoo Gyeong-Seon
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.18 no.1
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    • pp.43-52
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    • 2006
  • Numerical model tests are done in order to evaluate impact zone of low salinity water on outer region of the developing Saemankeum reservoir. Also saline mixing processes are investigated f3r the inner reservoir with consideration of Mankyoung and Donjin riverine flood discharges when sea water is passing freely through gate. In these analyses 2-d ADCIRC, 3-d TIDED3D and CE-QUAL-ICM models are used. Through models tests, it is found that inner reservoir mixing process caused by inflow of outer sea water occurs gradually. It takes at least one month for complete mixing on Mankyoung part and 6 month on Dongjin part of the reservoir. When Sinsi or Garyeok gates are opened to control inner reservoir level, discharging velocities decrease exponentially from the gates, but show very strong currents of 0.5m/sec to the 10Km region apart. These results imply that hydrodynamic circulation and ecosystem of frontal region of the Saemankeum dike might be affected in amount by gate operations, since low saline inner waters are discharged periodically at ebb tide according to tidal level.

Fine Structural Study on the Salivary Glands in the Pine Moth, Dendrolimus spectabilis Butler at the Last Larval Period (솔나방(Dendrolimus spectabilis Butler) 종령유충(終齡幼蟲)의 타액선(唾液腺)에 관한 미세구조적(微細構造的) 연구(硏究))

  • Baek, Seung-Bum;Moon, Myung-Jin;Kim, Woo-Kap
    • Applied Microscopy
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    • v.18 no.2
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    • pp.153-166
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    • 1988
  • The fine structure of the salivary glands of the pine moth, Dendrolimus spectabilis Butler, at the last larval period is observed using light and electron microscopes. The moths have single paired tubular salivary glands which openings are connected to the oral cavity through the upper jaw. By the external morphology and its functions, the glands are subdivided into three regions which are anterior reabsorptive region, middle storage region and posterior secretory region. Along the inner canal of the salivary gland two columns of the large glandular cells are connected each other and oriented to ring-like forms. By this cellular orientation, the glands have long and large tubular structure. From anterior to posterior region large nuclei of the glands are ramified like twigs of the tree, and in the cytoplasm of the cell numerous mitochondria and vacuoles are seen. Moreover, basal plasma membranes of the gland cells are heavily infolded. The anterior region of the glands keeps several characteristics related to the reabsorption of the material from the inner cavity to the glandular cells whereas, main salivary material is synthesized and secreted through the long and convoluted posterior region. The apical plasma membranes of the cells are the most heavily invaginated at the posterior regoin, but trachea and tracheoles are distributed only at the middle and posterior regions. In the cytoplasm of the middle region Golgi complexes appeared at the vicinity of the vesicles, and at the posterior region of the salivary glands multivesicular bodies are also observed.

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Diagnosis of the Transitional Disk Structure of AA Ori by Modeling of Multi-Wavelength Observations

  • Kim, Kyoung Hee;Kim, Hyosun;Lee, Chang Won;Lyo, Aran
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.42.2-42.2
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    • 2020
  • We report on multi-wavelength observations of AA Ori, a Young Stellar Object in Orion-A star-forming region. AA Ori is known to have a pre-transitional disk based on infrared observations including Spitzer/IRS data. We construct its broadband spectral energy distribution (SED) by not only taking data in the optical and IR region but also including Herschel/PACS, JCMT/SCUBA, and SMA observational data. We use the Monte Carlo radiative transfer code (RADMC-3D) to reconstruct the SED with a viscous accretion disk model initialized by a radially continuous disk and finally having an inner and outer dusty disk separated by a dust-depleted radial gap. By comparing the model SEDs with different configurations of disk parameters, we discuss the limits to find a single solution of model parameters to fit the data. We suggest that some models with a modified inner disk surface density gradient and some degree of dust depletion in the inner disk can explain the AA Ori's SED, from which we infer that the inner disk of AA Ori has evolved. We present that model configurations of a pre-transitional disk with a large gap extended to 60-80 AU in a settled dusty disk of a few hundred AU size with a high inclination angle (~60°) also create model SEDs close to the observed one. To distinguish whether the disk has a just-opened narrow gap or a large gap, with an altered surface density of the inner disk extended to 10 AU, we suggest a further investigation of AA Ori with high angular resolution observations.

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Association of CYP2E1 and NAT2 Polymorphisms with Lung Cancer Susceptibility among Mongolian and Han Populations in the Inner Mongolian Region

  • Zhang, Jing-Wen;Yu, Wan-Jia;Sheng, Xiao-Min;Chang, Fu-Hou;Bai, Tu-Ya;Lv, Xiao-Li;Wang, Guang;Liu, Su-Zhen
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.21
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    • pp.9203-9210
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    • 2014
  • Purpose: To explore associations of CYP2E1 and NAT2 polymorphisms with lung cancer susceptibility among Mongolian and Han populations in the Inner Mongolian region. Materials and Methods: CYP2E1 and NAT2 polymorphisms were detected by PCR-RFLP in 930 lung cancer patients and 1000 controls. Results: (1) Disequilibrium of the distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations (p=0.031). (2) Lung cancer risk was higher in individuals with c1, D allele of CYP2E1 RsaI/PstI, DraI polymorphisms and slow acetylation of NAT2 (c1 compared with c2, OR=1.382, 95%CI: 1.178-1.587, p=0.003; D compared with C, OR=1.241, 95%CI: 1.053-1.419, P<0.001; slow acetylation compared with rapid acetylation, OR=1.359, 95%CI:1.042-1.768, p=0.056) (3) Compared with c2/c2 and rapid acetylation, c1/c1 together with slow acetylation synergetically increased risk of lung cancer 2.83 fold. (4) Smokers with CYP2E1 c1/c1, DD, and NAT2 slow acetylation have 2.365, 1.916, 1.841 fold lung cancer risk than others with c2/c2, CC and NAT2 rapid acetylation, respectively. (5) Han smokers with NAT2 slow acetylation have 1.974 fold lung cancer risk than others with rapid acetylation. Conclusions: Disequilibrium distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations. Besides, Han smokers with NAT2 slow acetylation may have higher lung cancer risk compared with rapid acetylation couterparts. CYP2E1 c1/c1, DD and NAT2 slow acetylation, especially combined with smoking, contributes to the development of lung cancer. CYP2E1 c1/c1 or DD genotype and NAT2 slow acetylation have strong synergistic action in increasing lung cancer risk.

Inner disk properties of a Class I young stellar object revealed by IGRINS

  • Lyo, A-Ran;Kim, Jongsoo;Byun, Do-Young;Kang, Jihyun
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.2
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    • pp.35.4-36
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    • 2015
  • Gaseous inner disks are the main controller of the final structure of planetary systems as well as the building place of planets, especially of terrestrial planets. However, the inner disk of <5AU is still difficult to be spatially resolved even at the closest star forming regions. Resolving velocity structure in the disk with high resolution infrared spectroscopic study is the best approach to study the inner disk at this moment. Here, we present the IGRINS (Immersion GRating INfrared Spectrometer) result of the Class I young stellar object, ESO Ha 279a, in the Serpens molecular cloud region. IGRINS has a resolving power of R=40,000, corresponding to the velocity resolution of 7 km/s at K-band, which is perfect to study the hot inner disk structure. We report that NaI and CO overtone emission lines are indeed good tracers of the rotating inner warm disk tracing from ~0.04 to ~7AU of this source. We also report the disk properties using other emission lines.

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