• Bulletin of the Korean Chemical Society
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• 제35권10호
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• pp.2995-3000
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• 2014

A sensitive concentration method utilising modified gas-purge microsyringe extraction (GP-MSE) was developed. Concentration (reduction in volume) to a microlitre volume was achieved. PAHs were utilised as semivolatile analytes to optimise the various parameters that affect the concentration efficiency. The injection rate and temperature were the key factors that affected the concentration efficiency. An efficient concentration (75.0-96.1%) of PAHs was obtained under the optimised conditions. The method exhibited good reproducibility (RSD values that ranged from 1.5 to 9.0%). The GP-MSE concentration method enhances the volume reduction (concentration factor), leading to a low method detection limit ($0.5-15ngL^{-1}$). Furthermore, this method offers the advantage of small-volume sampling, enabling even the detection of diurnal hourly changes in the concentration of PAHs in ambient air. Utilising this method in combination with GC-MS, the diurnal hourly flux of PAHs from the gas phase of ambient air was measured. Indeed, the proposed technique is a simple, fast, low-cost and environmentally friendly.

• Archives of Pharmacal Research
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• 제29권6호
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• pp.514-519
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• 2006

A simple HPLC method using UV detection was developed and validated for the determination of levodropropizine (LDP) In dog plasma. The sample was prepared for injection using a liquid-liquid extraction method with 1-phenypiperazine as the internal standard. The mobile phase was methanol - diethylamine solution (0.05 M) (20:80, v/v, pH adjusted to 3.0 with $H_3PO_4$) with a detection wavelength of 240 nm. The limit of quantitation (LOQ) of LDP in a biological matrix was determined to be 25.25 ng/mL. The calibration curve was linear across the concentration range of 25.25 to 2020 ng/mL. The intra-day and inter-day precision values (CV%) were within 7% and accuracy (R.E. %) was within 6% of the nominal values for medium (252.5 ng/mL) and high (2020 ng/mL) LDP concentrations. For the LDP concentration at the LOQ, the intra-day and inter-day precision and accuracy were within 20% and 10%, respectively. The average absolute recovery for LDP was 70.28%. This method was successfully used to analyze plasma samples in a steady-state bioavailability study of a newly developed sustained-release LDP tablets (SR) using immediate-release tablets (IR) as the reference. The relative bioavailability of the SR was determined to be $106.3\;{\pm}\;12.8%$ (n=6). The $C_{max}$ of the SR was significantly lower (p<0.05), and the $t_{max}$ was significantly longer than that of the IR (p<0.05). The results of ANOVA and two one-sided tests indicated that the SR exhibited acceptable sustained release properties and was bioequivalent to the IR.

• International Journal of Automotive Technology
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• 제6권2호
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• pp.107-117
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• 2005

A distributed hardware-in-the-loop simulation (HILS) platform is developed for designing an automotive engine control system. The HILS equipment consists of a widely used PC and commercial-off-the-shelf (COTS) I/O boards instead of a powerful computing system and custom-made I/O boards. The distributed structure of the HILS system supplements the lack of computing power. These features make the HILS equipment more cost-effective and flexible. The HILS uses an automatic code generation extension, REAL-TIME WORKSHOP$^{ (RTW$^{) of MATLAB$^{ tool-chain and RT-LAB$^{, which enables distributed simulation as well as the detection and generation of digital event between simulation time steps. The mean value engine model, which is used in control design phase, is imported into this HILS. The engine model is supplemented with some I/O subsystems and I/O boards to interface actual input and output signals in real-time. The I/O subsystems are designed to imitate real sensor signals with high fidelity as well as to convert the raw data of the I/O boards to the appropriate forms for proper interfaces. A lot of attention is paid to the generation of a precise crank/ earn signal which has the problem of quantization in a conventional fixed time step simulation. The detection of injection! command signal which occurs between simulation time steps are also successfully compensated. In order to prove the feasibility of the proposed environment, a simple PI controller for an air-to-fuel ratio (AFR) control is used. The proposed HILS environment and I/O systems are shown to be an efficient tool to develop various control functions and to validate the software and hardware of the engine control system.

• 대한수의학회지
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• 제35권3호
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• pp.471-480
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• 1995

The purpose of this experiment was to develop a simple and reliable HPLC method for the detection of ciprofloxacin in chicken serum and to provide a basic data on pharmacokinetic parameters after oral and intramuscular administration. The results obtained were as follows: 1. 0.2% meta-phosphoric acid: acetonitrile(7:3, v/v) solution had a high and regular recovery rates and was selected as an extraction solution. 2. The recovery rates of ciprofloxacin were 83-97% with the selected solution in chicken serum and the detection limit was 50ng/ml in serum. 3. Ka(abosorption rate constant) were 3.652 1/h in fasted group and 0.880 1/h in non-fasted group, and Ke (elimination rate constant) were 0.061 1/h and 0.133 1/h, respectively. 4. The highest concentration in serum after intramuscular injection was 840ng/ml within 15-30min and 160-324ng/ml in 1.1-3.2 hours after oral administration. 5. The time course of blood concentration fits well into a 2 compartment model. 6. On oral administration of ciprofloxacin with feed, ciprofloxacin was absorbed more slowly and the amount of absorbed was smaller than that of in fasted chickens. 7. Blood concentration of ciprofloxacin increased in a dose-dependent manner after intramusclular and oral administraiton.

• 정보처리학회논문지:컴퓨터 및 통신 시스템
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• 제5권9호
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• pp.275-284
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• 2016

원자력분야에서 사용되는 안전관련 소프트웨어는 계획단계부터 설치단계까지의 전 생명주기 공정을 통해 개발과 확인검증, 안전성 분석, 그리고 품질보증 활동을 수행해 소프트웨어의 안전성을 보장하고 있다. 그러나 이러한 개발과 검증공정을 통한 평가는 시간과 비용을 많이 필요로 한다. 또한, 소프트웨어의 품질을 향상시키기 위해 다양한 활동을 수행했다고 주장하지만, 어느 정도의 품질이 향상되었는지 확인하기에는 한계가 있다. 이러한 한계를 극복하기 위해서 정량적인 평가를 수행할 수 있는 소프트웨어 신뢰도 계산 방법을 제안한다. 특히, 소프트웨어가 사용하는 메모리 공간에 고장을 주입하여 소프트웨어의 고장을 모사하고, 주입된 고장에 가중치를 부여하여 고장 민감도에 차이를 두고, 감지능력을 평가하여 소프트웨어 고장율을 계산한다. 이러한 고장율을 활용하여 소프트웨어 신뢰도 계산을 수행하면 정량적인 평가결과를 획득할 수 있게 된다.

• 한국정보통신학회논문지
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• 제24권8호
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• pp.1077-1085
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• 2020

부설된 기뢰를 소해하기 위해 기뢰매설 예상구역에 대한 탐색을 수행한다. 이 때 기뢰탐색은 기뢰의 위험성, 아군의 안정성 등을 고려하여 자율무인잠수정을 이용한다. 매설된 기뢰를 식별하기 위한 소나시스템은 측면주사소나, 합성개구소나 등을 탑재한다. 본 논문은 측면주사소나 특성에 따른 기뢰탐색효과도 분석에 대해 기술한다. 각 측면주사소나의 특성을 바탕으로 음향조사역 및 인식확률을 모델링 하였고, AUV의 주행패턴에 따라 분석을 수행하였다. AUV의 주행패턴은 측면주사소나 음영구역의 유무에 따라 3가지 탐색패턴을 정의하였다. 분석결과는 각 탐색패턴 마다 탐지시간, 탐지확률을 도출하고 최종적으로 측면주사소나 음영구역의 유무에 따른 탐색 향상도를 도출하였다.

• 정보보호학회논문지
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• 제22권2호
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• pp.283-293
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• 2012

최신의 변종 악성코드는 백신에 의해 쉽게 탐지 되지 않아 장시간에 걸쳐 개인정보와 같은 다양한 데이터를 유출시키기도 한다. 일반적으로 인젝션, 취약한 인증과 세션관리, 크로스 사이트 스크립팅(XSS), 안전하지 않은 직접 객체 참조 등의 웹 취약점을 활용한 거점 좀비 PC를 이용하여 네트워크 연계를 통하여 정보 유출형 악성코드가 빈번히 설치되고 있다. 악성코드가 실행되면 임의의 서비스와 프로세스로 등록되고, 등록된 악성코드는 이를 기반으로 공격자가 정보를 수집하는 사이트로 주기적으로 정보를 유출한다. 본 논문에서는 2011년 1월부터 빈번하게 발생한 악성코드 중에서 유용한 사례로 웜 형태의 신종 악성코드인 소위 "winweng"의 체계적인 분석을 통해서 감염경로 및 정보유출의 과정과 방법에 대하여 분석하고 이에 대한 대응 방안을 연구하였다.

• 한국산업융합학회 논문집
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• 제25권3호
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• pp.443-449
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• 2022

In this study, in order to improve the disadvantages of the environmental error of the infusion set that performs infusion therapy in the existing clinical practice and to maximize the user's convenience by miniaturizing the existing infusion pump system, the structure of the muscle pump of the human vein was imitated. As a double check valve method, a method for preventing the backflow of fluid and discharging a constant fluid in one direction by external pressure was proposed. The proposed bio-mimic muscle pump uses a check valve that controls the flow of fluid in one direction and a silicone tube with elasticity, and a chamber is constructed. A peristaltic pump for applying intermittent pressure to the tube chamber was constructed using a multi-cam structure roller. In order to verify the performance of the proposed pump, optimization was performed while changing the number of multi-cam rollers and adjusting the speed of the roller driving motor, and the reproducibility of the instantaneous discharge amount and the continuous discharge amount of the pump was compared and tested. The performance of the muscle pump proposed in this study was verified through experiments that it can inject up to 1L of fluid within 12 hours, and that it is possible to inject the fluid with an accuracy of ±0.1ml. Real-time monitoring of the fluid injection volume through the bio-mimic muscle pump proposed in this study not only increases the convenience of the administrator, but also provides a precise fluid administration environment to more patients at a low cost, and additionally applies bubble detection and occlusion detection technology If so, it is believed that a safer medical environment can be provided to patients.

• Animal Bioscience
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• 제36권4호
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• pp.591-600
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• 2023

Objective: We aimed to evaluate the efficacy of a modified Double-Ovsynch protocol vs artificial insemination following estrus detection (AIED) for the enhancement of reproductive performance in Hanwoo cattle. Methods: Four hundred twelve Hanwoo cows were allocated to two treatment groups. The first group of cows were administered gonadotropin releasing hormone (GnRH) on Day 36 (±0.6), prostaglandin F_2α (PGF_2α) on Day 46 (8 to 12 days later), and GnRH on Day 49, which was followed by Ovsynch, consisting of an injection of GnRH on Day 56, PGF_2α on Day 63, and GnRH 56 h and timed AI (TAI) 16 h later (modified Double-Ovsynch group, n = 203). The second group of cows underwent AIED (AIED group, n = 209) and were designated as controls. Results: The pregnancy per AI 60 days after the first AI was higher in the modified Double-Ovsynch (68.5%) than in the AIED (56.5%) group, resulting in a higher probability of pregnancy per AI (odds ratio: 1.68, p<0.05). Moreover, cows in the modified Double-Ovsynch group were more likely (hazard ratio: 1.28, p<0.05) to be pregnant by 150 days after calving than cows in the AIED group, and this difference was associated with a lower mean number of AIs per conception (1.27 vs 1.39, p<0.05) and a shorter median interval between calving and pregnancy (72 vs 78 days, p<0.1). Conclusion: The modified Double-Ovsynch protocol, adjusted according to the herd visit schedule, can be readily used to increase the pregnancy per AI following the first AI and to shorten the interval between calving and pregnancy in beef herds.

• Journal of Life Science
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• 제10권1호
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• pp.24-27
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• 2000

For the rapid diagnosis of vibriosis in penaeid shirmp, the indirect fluorescent antibody technique(IFAT) was established to detect Vibrio alginolyticus. The titers of the antisera used for this experiment were above 1280. Vibrio alginolyticus possesses the specific antigen, and also have antigens shared with other strains. When an V. alginolyticus-infected adult shirmp was tested by IFAT, V. alginolyticus was detected mainly in the muscle tissues near the injection point and the haemolymph but only few in other tissues. This result indicates that the pathogen bacteria could be detected by IFAT. Thus, it is suggested IFAT is more convenient and sensitive method than conventional plate method for the diagnosis of induced Vibrio infection in the penaeid shrimps.