• Natural Product Sciences
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• v.2 no.2
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• pp.137-142
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• 1996

This paper gives a description about the germination inhibition of American ginseng (Panax quinquefolium L.) seed coat. The existence of seed coat is one of the inhibitory factors which inhibit the embryo growth, particularly during the morphological after-ripening stage. The seed coat can obstruct the water absorption at the beginning of seed stratification, but it can not threaten seed germination. The inhibition of seed coat is not caused by the mechanical fetter neither. However, before splitting the seed coat, the inhibition of seed coat comes from both air-tight character and inhibitors, and after splitting the seed coat, the inhibition may come mainly from the inhibitors.

• The Korean Journal of Ecology
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• v.20 no.4
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• pp.233-238
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• 1997

To evaluate allelopathic effect of volatile chemicals and phytohormones, seed germination and seedling growth test of Lactuca sativa have performed in laboratory experiments. Among used chemicals terpienen-4-ol was the most inhibitory to seed germination of lettuce. ABA and GA inhibited seed germination at $5{\times}10^{-6}M$ concentration but promoted germination at $2.5{\times}10^{-5}M$ and $5{\times}10^{-5}M$. ABA and GA alleviated volatile chemical-induced inhibition of seed germination and seedling elongation of lettuce.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.2
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• pp.134-138
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• 2000

Most parts of alfalfa plant have been reported to contain autotoxic substances that inhibit seed germination and early seedling growth, however, the chemical(s) is not still studied much. Effect of seed leachates of 'Vernal' alfalfa (Medicago sativa L.) was evaluated for inhibition of alfalfa germination and root growth through bioassay. Alfalfa seeds were extracted in 1 L deionized water for 1 h after soaking and the leachates caused to reduce root length of alfalfa significantly as the soaking time increased. Crude seeds at 4 g L$L^{-1}$ exudated autotoxins that reduce significantly root length by 34 % compared to the control, when the seeds soaked in deionized water for 24 h. However, the extracts did not affect final germination as well as speed of germination. Extracts from ground seeds significantly reduced speed of germination (GT 50) and root length. The results indicate that release of autotoxic substances from seeds during seed imbibition was increased with increase of soaking time and seed amount, and that autotoxicity was more occurred in ground seeds than in crude seeds.

• Journal of Microbiology and Biotechnology
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• v.17 no.4
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• pp.586-593
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• 2007

Seed coating by a phenazine-producing bacterium, Pseudomonas chlororaphis O6, induced dose-dependent inhibition of germination in wheat and barley seeds, but did not inhibit germination of rice or cucumber seeds. In wheat seedlings grown from inoculated seeds, phenazine production levels near the seed were higher than in the roots. Deletion of the gacS gene reduced transcription from the genes required for phenazine synthesis, the regulatory phzI gene and the biosynthetic phzA gene. The inhibition of seed germination and the induction of systemic disease resistance against a bacterial soft-rot pathogen, Erwinia carotovora subsp. carotovora, were impaired in the gacS and phzA mutants of P chlororaphis O6. Culture filtrates of the gacS and phzA mutants of P. chlororaphis O6 did not inhibit seed germination of wheat, whereas that of the wild-type was inhibitory. Our results showed that the production of phenazines by P. chlororaphis O6 was correlated with reduced germination of barley and wheat seeds, and the level of systemic resistance in tobacco against E. carotovora.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.51-59
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• 2008

Lettuce, a typical light-induced seed germination type, exhibits different germination responses according to cultivars, light quality, and plant growth regulator (PGR) treatments. Germination rates in most tested cultivars were over 85% under both white and red light, and were slightly decreased by blue light. Although photo-inhibition in germination was observed from most cultivars by far-red light, 'Cheongguangcheongchima', 'Okdol', and 'Manchudaecheongchima' could be classified as photo-insensitive lettuce cultivars by exhibiting the germination rates as 78,63, and 48% under for-red light, respectively. 6-Benzylamino purine (BAP) and kinetin promoted seed germination and normal seedling production under far-red light, but ethephon did not show any positive effects. Cytokinins such as BAP, kinetin, thidiazuron (TDZ), and zeatin overcame photo-inhibition of seed germination even the concentration of below $50\;mg{\cdot}L^{-1}$. However, auxins such as IBA, 2,4-D, and NAA failed to overcome the far-red light-induced photo-inhibition.

• Journal of Biosystems Engineering
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• v.40 no.3
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• pp.224-231
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• 2015

Purpose: Corn is a major commercial crop targeted for genetic modification owing to its high consumer demand as a foodstuff for humans and livestock, as well as its other industrial applications. However, the safety of genetically modified (GM) crops is controversial. Indeed, several countries have banned the importation of GM seeds that can germinate. Therefore, development of effective, convenient, and nondestructive methods to inhibit seed germination is required. Methods: This study aimed to examine the efficacy of microwave heat treatment for inhibition of germination of corn kernels and for optimization of power and exposure time required for effective aging treatment. Artificial inhibition was induced in corn kernels using microwave heat treatment. Seven power levels were examined (400, 500, 600, 700, 800, 900, and 1000 W) at each of the four exposure times (0.5, 1.0, 1.5, and 2.0 min). Results: Corn kernels could be aged effectively after heating for 0.5~1.0 min at powers greater than 800 W, with increasing efficacy observed at higher powers. Further analysis showed that the most effective inhibition of germination was observed at 1000 W for 40 s. This setting did not cause any physical damage to the corn kernels. Conclusions: Optimal inhibition of corn kernel germination was achieved using higher power for shorter times, which may be useful for industrial corn seed treatment.

• The Plant Pathology Journal
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• v.26 no.3
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• pp.245-252
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• 2010

An endophytic bacterial strain, Arthrobacter humicola YC6002, was isolated from a surface sterilized root of Korean turf grass (Zoysia japonica) collected from Jinju, Korea. This strain showed inhibitory effect on germination and shoot growth of radish. The inhibition of germination and shoot growth of radish seeds varied depending on the age of culture and the temperature at which it was incubated. The culture filtrate of 1/10-strength Tryptic Soy Broth medium, incubated for 48 hours at $30^{\circ}C$, showed the highest inhibitory effect on radish seed germination and shoot growth (92% inhibition as compared to control). The active compound with seed germination and shoot growth inhibition was purified and identified as 3-phenylpropionic acid. The purified compound had 53% and 93% inhibitory effect on seed germination and shoot growth of radish for 500 and 1000 ppm solutions, respectively.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.4 no.1
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• pp.59-69
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• 2000

The present study was undertaken in investigate the response to salinity and effect of plant growth regulators and proline under salinity stress on the germination and seedling growth of Vigna angularis. The protective effect of external Ca2+ on root elongation under saline conditions was also investigated. The seed germination of Vigna angularis decreased with an increase in salinity. The growth regulators GA3 was more effective than kinetin. At a higher salinity, low concentrations of kinetin and high concentrations of GA3 were more effective. The external application of proline and betaine improved germination under saline conditions. At a low salinity proline and betaine alleviated the salinity-induced inhibition of germination, yet at higher NaCl concentrations, proline and betaine were both ineffective. Exposure to salinity during germination was accompanied by an increase in the proline content, thereby suggesting that one compatible solute in the germinating seed would seem to be proline. The inhibition of germination by high NaCl concentrations was relatively more severe in scarified seeds than in intact seeds, indicating that the seed coat acts as a partial barrier to an Na2+ ameliorated the adverse effect of salinity stress.

• Journal of Plant Biology
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• v.19 no.1
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• pp.1-6
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• 1976

This study was carried out to investigate the formation of precipitates between lead ion and the essential anions of plants, the effects of lead concentration on seed germination and plant growth in water and soil culture, and the germinating and growing recovery of inhibited seed germination and plant growth by lead. Four kinds of the seeds (Glycine max M., Triticum vulgare V., Setaria viridis (L) P. De Beauvois, and Digitoria sanguinalis (L) Scopoli var) were germinated and growth in water and soil culture included the different concentrations of lead for five days. The seeds and plants inhibited germination and growth by lead were transferred to lead free Hoagland solution and the growing recovery was observed. The precipitates of lead ion were observed in the solution of both acidity and alkalinity included each anion of $H_2PO_4^-, HPO_4^{2-}, PO_4^{3-}, SO_4^{2-} and MoO_4^{2-}$ in a room temperature, whereas the precipitates between lead ion and other anions were observed largely in the solution of alkalinity, so that it seemed that lead could be remained in the state of non-soluble in plant and soil. The inhibition of germination and growth in the water culture was observed in 100ppm of lead, whereas the inhibition in the case of the soil culture was observed in 10000ppm of lead. The difference of the effected concentration between water and soil culture in germination and the growth was 100 times. When the seed and plant inhibited the growth in 5000ppm or 10000ppm of lead for five days were transferred to lead free Hoagland solution, the recovery of germination and growth was observed in three days. This growing recovery was different according to the kinds of plant and concentrations of lead. It seemed that plant growth could be inhibited by the inhibition of the metabolism concerned with the precipitates between lead iion and other anions.

• Journal of Forest and Environmental Science
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• v.33 no.3
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• pp.226-232
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• 2017

Dhup (Canarium resiniferum) is an economically and aesthetically important rare native tree species of Bangladesh. In natural condition 78.5-98.7% seeds do not germinate due to inhibition by hard seed coat, seed predation and unfavorable environmental conditions. A study was conducted in the Seed Research Laboratory and nursery of Institute of Forestry and Environmental Sciences, Chittagong University, Bangladesh in 2013 to find out appropriate pre-sowing treatments for maximizing germination and initial seedling growth. Eleven pre-sowing treatments were provided in both the seeds sown in polybags and seeds sown in propagator house. Results revealed that, germination started at first (after 20 days of seed sown) in seeds immersed in water at room temperature for 24 hours and germination completed within 38 days. Significantly higher (p<0.05) germination percentage (33%), germination energy (16.7%), plant percent (33%) and germination value (0.4) was found with seeds immersed in water at room temperature for 24 hours. Seedling height measured at three, four and five months after the seed germination in case of seeds treated by immersion in water for 24 hours was also greater than others. Therefore, pre-sowing treatment by immersion in water at room temperature for 24 hours was more effective in germination and production of quality vigor seedlings of Dhup.