• 제목/요약/키워드: inhibiting activity

검색결과 1,310건 처리시간 0.034초

전기분해수와 이산화염소수 처리 복분자(Rubus coreanus Miquel)의 살균효과 (Sterilization effect of electrolyzed water and chlorine dioxide on Rubus coreanus Miquel)

  • 텅후이;김유호;이원영
    • 한국식품저장유통학회지
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    • 제20권4호
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    • pp.459-466
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    • 2013
  • 본 연구에서는 수도수, 전기분해수, 이산화염소수 처리에 의한 복분자 딸기의 저장온도 및 기간에 따른 품질 변화를 분석하였다. 복분자 딸기를 수도수, 전기분해수와 이산화염소수 농도별 10, 50, 100, 200 ppm으로 각각 30초 동안 침지를 한 후, 상온과 냉장 저장하여 시간에 따른 저장성 효과를 비교하였다. 또한, 외관 품질유지와 갈변저해효과를 탐색하였다. 또한, 복분자로부터 Bacillus cereus와 Micrococcus luteus의 위해미생물을 분리 동정하였다. 살균세척수를 처리한 후 저장 온도별 시간에 따른 미생물 총균수를 측정한 결과, 각 처리구의 미생물 살균력은 전기분해수와 이산화염소수가 수도수 처리보다 증가하였으며 이산화염소수는 고농도일수록 현저하게 증가하였다. 상온에서 저장하는 구보다 냉장 저장한 구에서의 미생물 억제효과가 뛰어났다. 복분자 딸기의 표면에 주사전자현미경으로 관찰한 결과, 전기분해수와 이산화염소수 처리구에서는 미생물이 생존하지 않았다. 효소활성을 측정한 결과, 수도수 처리구보다 살균세척수 처리구에서 함량이 낮아졌으며 시간이 지남에 따라 서서히 증가하였다. 총 폴리페놀 함량은 세척 방법 간 유사한 결과를 나타내었다. 저장일수가 증가할수록 감소하였고 전기분해수와 이산화염소수 처리구의 함량이 수도수 처리보다는 낮았다. 따라서, 복분자 딸기의 전기분해수와 이산화염소수 처리는 미생물 생육을 억제하면서 신선도를 유지하고 품질 유지 및 저장성 향상에 기여할 것으로 사료된다.

천연기념물 식물유전자원의 보존방안 고찰 - 나고야의정서 발효 대비 중심으로 - (Consideration of preservation methods for plant genetic resources in natural monument - Focusing on preparation for becoming effective of Nagoya Protocol -)

  • 김정아;김효정;신진호;김대열;조운연
    • 농업과학연구
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    • 제41권3호
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    • pp.193-203
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    • 2014
  • Natural Monument is a designated cultural property as part of the country. According to Article 2 of the Cultural Properties Protection Act, a national, ethnic and global heritage artificially or naturally formed, with a great historical, artistic, scientific and landscape significance is defined as a cultural heritage. Animals, plants, topography, geology, minerals, caves, biological products and special natural phenomena, having a great of historic, scenic and scientific value, are defined as the monument. According to Article 3 of Cultural Properties Protection Act, the conservation, management and utilization of National Heritage should be kept intact in its original form. So, Natural monuments are managing as retained its original form under the Basic Principles of current law. The highest population of coniferous tree in natural monument plant is ginkgo tree including 22 objects, followed by pines, junipers that order. And in case of broadleaf tree, there are zelkova trees, retusa fringe trees, pagoda trees, cork oaks, silver magnolias and etc. There are many of reported efficacy in available natural monument plants. The efficacy of plant species on pharmaceutical like anti-cancer, anti-diabetic, anti-obesity, antioxidant activity, neuroprotective, improves cholesterol, anti-inflammatory, liver protection and anti-bacterial efficacy, on cosmetics and beauty like the inhibiting formation of skin wrinkles, whitening effect, variety of materials and the efficacy of the proposed utilization of its various papers and etc have been widely reported. Before the Nagoya Protocol enters into force, the future role of the National Research Institute for Cultural Properties Administration of Cultural Heritage should be obtain a legal right to manage the social, cultural and national natural monument with emotional value to the plant genetic resource as a natural monument efficient ways to study and preserve traditional knowledge biological resources by securing a claim to the sovereignty of the material will be ready.

무기물성 및 식물성 생리활성 물질이 반추위 미생물의 성장에 미치는 영향 (Effects of Biologically Active Materials Prepared for Several Minerals and Plants on the Growth of Rumen Microbes)

  • 신성환;이신자;옥지운;이상민;임정화;김경훈;문여황;이성실
    • 생명과학회지
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    • 제17권11호
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    • pp.1555-1561
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    • 2007
  • 본 연구는 무기물성 및 식물성 유래 생리활성물질로서 scoria, germanium, charcoal, 생강, stevia 및 CLA(conjugated linoleic acid)가 병원성 미생물 및 반추위 미생물에 대한 작용을 조사하기 위하여 수행되었다. 병원성 균으로서 Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes 및 Staphylococcus aureus에 적용하여 항균활성을 측정하고, in vitro 발효 시 반추위 미생물 성장률, 가스 생성량, 암모니아 농도, CMCase 활성 및 미생물의 수를 측정하였다. 병원성 미생물 배양액에 생강을 0.1% 첨가한 구에서만 항균활성이 나타났으나, paper disc법에 의한 항균활성 시험에서는 stevia 10%첨가구와 CLA 10% 첨가구에서 E. coli에 대해서 항생제 첨가구인 positive control 구와 비슷한 크기의 clear zone을 형성하였다. in vitro 반추위 미생물 발효시험에서는 생강, stevia 및 CLA가 반추위 박테리아와 프로토조아의 증식을 억제하는 것으로 나타났는데, 특히 생강 첨가구의 경우 메탄 생성균의 서식지로 알려져 있는 프로토조아를 크게 억제함으로써 메탄생성 억제제로서 개발 가능성이 있는 것으로 사료된다.

O-GlcNAcylation of NF-κB Promotes Lung Metastasis of Cervical Cancer Cells via Upregulation of CXCR4 Expression

  • Ali, Akhtar;Kim, Sung Hwan;Kim, Min Jun;Choi, Mee Young;Kang, Sang Soo;Cho, Gyeong Jae;Kim, Yoon Sook;Choi, Jun-Young;Choi, Wan Sung
    • Molecules and Cells
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    • 제40권7호
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    • pp.476-484
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    • 2017
  • C-X-C chemokine receptor 4 (CXCR4) stimulates cancer metastasis. NF-${\kappa}B$ regulates CXCR4 expression in cancer cells, and O-GlcNAc modification of NF-${\kappa}B$ promotes its transcriptional activity. Here, we determined whether CXCR4 expression is affected by O-GlcNAcylation of NF-${\kappa}B$ in lung metastasis of cervical cancer. We found elevated levels of O-linked-N-actylglucosamine transferase (OGT) and O-GlcNAcylation in cervical cancer cells compared to those in non-malignant epithelial cells and detected increased expression of NF-${\kappa}B$ p65 (p65) and CXCR4 in cervical cancer cells. Knockdown of OGT inhibited the O-GlcNAcylation of p65 and decreased CXCR4 expression levels in HeLa cells. Thiamet G treatment increased O-GlcNAcylated p65, which subsequently enhanced CXCR4 expression levels. Inhibition of O-GlcNAcylation by 6-Diazo-5-oxo-L-norleucine (DON) treatment decreased p65 activation, eventually inhibiting CXCR4 expression in HeLa cells. Lung tissues from mice engrafted with OGT-knockdown HeLa cells (shOGT) exhibited lower expression of Ki-67 and HPV E6 and E7 oncogenes compared to lung tissues from mice engrafted with control HeLa cells (shCTL). In addition, lung tissues from mice engrafted with shOGT cells exhibited lower p65 and CXCR4 immunoreactivity compared to tissues from mice engrafted with shCTL cells. Taken together, our data suggest that p65 O-GlcNAcylation promotes lung metastasis of cervical cancer cells by activating CXCR4 expression.

복분자(覆盆子)가 노화유발(老化誘發) 흰쥐의 항산화능(抗酸化能)에 미치는 영향(影響) (Effect of Rubi Fructus(Fruit of Rubus Coreanus Miq.) on Antioxidant Capacity in D-galactose Induced Aging Rats)

  • 박성준;이송실;백진웅;이상재;김광호
    • 대한예방한의학회지
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    • 제8권1호
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    • pp.75-87
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    • 2004
  • Objectives: Rubi Fructus(fruit of Rubus coreanus Miq.) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect. The purpose of this study is to investigate the effect of Rubi Fructus(fruit of Rubus coreanus Miq.) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase(GSH-px) in rat erythrocytes and blood plasma. Methods: Sprague-Dawley rats were divided into 3 groups, Normal group(supplied enough water and feeds only, Normal Group), D-galatose administered group(injected D-galatose 50mg/kg, 1time/day for 6 weeks, Control Group) and Rubi Fructus (fruit of Rubus coreanus Miq.) administered group(D-galactose 50mg/kg and Rubi Fructus(fruit of Rubus coreanus Miq.) extracts 85.0mg/200g 1time/day for 6 weeks, BBJ Group). Rats were sacrificed and TBARS, SOD, CAT, GSH-px, Plasma total lipid, Plasma triglyceride and cholesterol were measured in rat erythrocytes and blood plasma. Results : Plasma TBARS concentrations of all experimental group were not significantly different. Red blood cell(RBC) SOD activities of BBJ group was increased, and RBC catalase activities of all experimental group were not significantly different. RBC GSH-px activities of BBJ group was increased. Plasma total lipid concentration of BBJ group were significantly lower than those of control. Plasma triglyceride, total cholesterol and HDL-cholesterol concentrations of all experimental group were not signi ficantly different. Conclusions: According to the above results, it is considered that Rubi Fructus(fruit of Rubus coreanus Miq.) is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

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대맥근(大麥根)의 생장(生長)과 Transaminase의 활성(活性)에 미치는 몇 가지 질소화합물(窒素化合物)의 영향(影響) (Effects of Various Nitrogen Compounds for the Growth of Barley Roots and Transaminase Activity)

  • 김광식
    • 한국토양비료학회지
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    • 제3권1호
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    • pp.43-48
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    • 1970
  • 질소급원(窒素給源)을 달리 한 수경액(水耕液)에 대맥(大麥)을 재배(栽培)하여 근부생장(根部生長)과 근부(根部)의 GOT GPT의 활성(活性)을 측정(測定)하여 근부(根部)의 생장(生長)과 Transaminase의 활성(活性)과의 관계(關係)를 구명(究明)코저 실험(實驗)하였든바 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1. $NO_3-N$를 공급(供給)한 대맥근(大麥根)의 생장(生長)은 $NH_4-N$를 공급(供給)한 것에 비(比)하여 생장(生長)이 양호(良好)하다. 2. $NH_4-N$를 공급(供給)한 대맥근(大麥根)은 $NO_3-N$를 공급(供給)한 근(根)에 비(比)하여 생장(生長)이 불량(不良)할 뿐만 아니라 억제적(抑制的)인 경향(傾向)이 있다. 3. $NH_4-N$를 공급(供給)하여 생장(生長)이 불량(不良)한 것은 GOT GPT 활성도(活性度)가 비교적(比較的) 높고, $NO_3-N$를 공급(供給)한 근(根)의 활성(活性)은 그것에 비(比)하여 높지 않다. 4. 수도근(水稻根)의 생장(生長)을 비교적(比較的) 억제(抑制)한다는 Amino산(酸)을 공급(供給)하여 생장(生長)이 양호(良好)한 대맥근(大麥根)은 GOT GPT 활성(活性)이 약(弱)하고, 생장(生長)이 불량(不良)한 것을 활성도(活性度)가 높다. 5. 대맥근부(大麥根部)의 세포(細胞) 각분획(各分劃)에 있어서 GOT GPT 활성도(活性度)는 대부분(大部分) Supernatant fraction(Microsomal fraction)에서 강(强)하며 Mitocondrial fraction은 극(極)히 미약(微弱)하다.

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과도 운동에 의해 유발되는 피로 및 골격근 산화적 손상에 대한 황기 다당체의 효과 (Effect of Polysaccharides from Astragalus membranaceus on Exercise-Induced Fatigue and Oxidative Damage in Skeletal Muscle in Exhaustive Exercise Animal Models)

  • 고은지;이한나;박현수;김수진;박영철;성은수;유창연;임정대
    • 한국약용작물학회지
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    • 제26권4호
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    • pp.271-280
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    • 2018
  • Background: Astragalus membranaceus is a well known oriental medicinal herb. The polysaccharides of the aboveground parts (AMA) and the radix (AMR) of A. membranaceus are the most important functional constituents. Methods and Results: The aim of this study was to determine the effects of AMA and AMR on the oxidative damage induced in the skeletal muscle of rats subjected to exhaustive exercise. Sprague-Dawley rats were randomly divided into exercise and non-exercise groups; in the groups receiving the test compounds, AMA and AMR were administered orally for 30 days. Skeletal muscle samples were collected from each rat after running to exhaustion on a treadmill to determine the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) and the concentation of malondialdehyde (MDA). The antioxidant enzyme activities of SOD and GSH-Px of skeletal muscle of AMA- and AMR-treated groups were significantly higher than those of the control and commercial sports drink (SPD)-treated groups in exhaustive exercise rats. In addition, MDA concentrations in the skeletal muscle of the AMA- and AMR-treated groups were significantly lower than those of the control and SPD-treated groups. In the present study, the effects of AMA and AMR on exercise endurance capacity were also evaluated in mice subjected to a swimming exercise test. AMA and AMR supplementation prolonged the swimming time of mice and enhanced exercise endurance capacity. AMA and AMR possess the ability to retard and lower the production of blood lactate, and prevent the decrease of serum blood glucose. Conclusions: These results showed that, AMR and AMA exerted beneficial effect in mice, increasing the activity of the antioxidant systems and inhibiting oxidative stress induced by exhaustive exercise. The compounds improved exercise performance and showed anti-fatigue effects against exhaustive exercise.

전립선 암세포에서 delphinidin에 의한 HIF-1α와 STAT3 억제를 통한 혈관내피 성장 인자 발현 저해 효과 (Delphinidin Suppresses Angiogenesis via the Inhibition of HIF-1α and STAT3 Expressions in PC3M Cells)

  • 김문현;김미현;박영자;장영채;박윤엽;송현욱
    • 한국식품과학회지
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    • 제48권1호
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    • pp.66-71
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    • 2016
  • 델피니딘은 양전하를 뛰는 diphenylpropane의 polyphenolic ring 구조를 가진 주요한 안토시아닌 색소 중에 하나이다. 최근 연구에서 델피니딘은 항산화, 항염증 뿐만 아니라 항암 효능을 가진다고 보고되었다. 본 연구에서는 전립샘 암에서 종양의 성장과 신생혈관생성에 관련된 중요한 인자인 VEGF 발현에 대한 델피니딘의 억제 효과를 조사하였다. RT-PCR을 통해 델피니딘을 처리한 PC3M 전립샘 암세포 세포에서 EGF로 유도한 VEGF mRNA 발현 수준이 감소됨을 확인하였다. 또한 델피니딘은 VEGF의 전사인자인 HIF-$1{\alpha}$와 STAT3가 세포 핵으로 전위되는 것을 효과적으로 억제하였다. 한편 luciferase assay을 통해 HRE-promoter 활성을 확인해 본 결과, 델피니딘이 HIF-$1{\alpha}$의 전사 활성을 억제시켜 VEGF 발현을 감소시키는 것을 알 수 있었다. 그리고 델피니딘은 EGFR의 발현에는 영향을 미치지 않고, Akt, p70S6K, 4EBP1의 인산화를 특이적으로 억제하는 것으로 나타났다. 결론적으로 델피니딘이 HIF-$1{\alpha}$와 STAT3, VEGF 발현을 억제를 통하여 암세포 증식억제와 신생혈관형성을 억제하는 역할을 새롭게 확인하였다.

Effects of 17β-Estradiol on Colonic Permeability and Inflammation in an Azoxymethane/Dextran Sulfate Sodium-Induced Colitis Mouse Model

  • Song, Chin-Hee;Kim, Nayoung;Sohn, Sung Hwa;Lee, Sun Min;Nam, Ryoung Hee;Na, Hee Young;Lee, Dong Ho;Surh, Young-Joon
    • Gut and Liver
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    • 제12권6호
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    • pp.682-693
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    • 2018
  • Background/Aims: Intestinal barrier dysfunction is a hallmark of inflammatory bowel diseases (IBDs) such as ulcerative colitis. This dysfunction is caused by increased permeability and the loss of tight junctions in intestinal epithelial cells. The aim of this study was to investigate whether estradiol treatment reduces colonic permeability, tight junction disruption, and inflammation in an azoxymethane (AOM)/dextran sodium sulfate (DSS) colon cancer mouse model. Methods: The effects of $17{\beta}$-estradiol (E2) were evaluated in ICR male mice 4 weeks after AOM/DSS treatment. Histological damage was scored by hematoxylin and eosin staining and the levels of the colonic mucosal cytokine myeloperoxidase (MPO) were assessed by enzyme-linked immunosorbent assay (ELISA). To evaluate the effects of E2 on intestinal permeability, tight junctions, and inflammation, we performed quantitative real-time polymerase chain reaction and Western blot analysis. Furthermore, the expression levels of mucin 2 (MUC2) and mucin 4 (MUC4) were measured as target genes for intestinal permeability, whereas zonula occludens 1 (ZO-1), occludin (OCLN), and claudin 4 (CLDN4) served as target genes for the tight junctions. Results: The colitis-mediated induced damage score and MPO activity were reduced by E2 treatment (p<0.05). In addition, the mRNA expression levels of intestinal barrier-related molecules (i.e., MUC2, ZO-1, OCLN, and CLDN4) were decreased by AOM/DSS-treatment; furthermore, this inhibition was rescued by E2 supplementation. The mRNA and protein expression of inflammation-related genes (i.e., KLF4, NF-${\kappa}B$, iNOS, and COX-2) was increased by AOM/DSS-treatment and ameliorated by E2. Conclusions: E2 acts through the estrogen receptor ${\beta}$ signaling pathway to elicit anti-inflammatory effects on intestinal barrier by inducing the expression of MUC2 and tight junction molecules and inhibiting pro-inflammatory cytokines.

Sodium butyrate reduces high-fat diet-induced non-alcoholic steatohepatitis through upregulation of hepatic GLP-1R expression

  • Zhou, Da;Chen, Yuan-Wen;Zhao, Ze-Hua;Yang, Rui-Xu;Xin, Feng-Zhi;Liu, Xiao-Lin;Pan, Qin;Zhou, Huiping;Fan, Jian-Gao
    • Experimental and Molecular Medicine
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    • 제50권12호
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    • pp.2.1-2.12
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    • 2018
  • Glucagon-like peptide-1 (GLP-1) has a broad spectrum of biological activity by regulating metabolic processes via both the direct activation of the class B family of G protein-coupled receptors and indirect nonreceptor-mediated pathways. GLP-1 receptor (GLP-1R) agonists have significant therapeutic effects on non-alcoholic fatty liver disease (NAFLD) and steatohepatitis (NASH) in animal models. However, clinical studies indicated that GLP-1 treatment had little effect on hepatic steatosis in some NAFLD patients, suggesting that GLP-1 resistance may occur in these patients. It is well-known that the gut metabolite sodium butyrate (NaB) could promote GLP-1 secretion from intestinal L cells. However, it is unclear whether NaB improves hepatic GLP-1 responsiveness in NAFLD. In the current study, we showed that the serum GLP-1 levels of NAFLD patients were similar to those of normal controls, but hepatic GLP-1R expression was significantly downregulated in NAFLD patients. Similarly, in the NAFLD mouse model, mice fed with a high-fat diet showed reduced hepatic GLP-1R expression, which was reversed by NaB treatment and accompanied by markedly alleviated liver steatosis. In addition, NaB treatment also upregulated the hepatic p-AMPK/p-ACC and insulin receptor/insulin receptor substrate-1 expression levels. Furthermore, NaB-enhanced GLP-1R expression in HepG2 cells by inhibiting histone deacetylase-2 independent of GPR43/GPR109a. These results indicate that NaB is able to prevent the progression of NAFL to NASH via promoting hepatic GLP-1R expression. NaB is a GLP-1 sensitizer and represents a potential therapeutic adjuvant to prevent NAFL progression to NASH.