• 한국응용곤충학회지
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• 제38권3호
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• pp.255-260
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• 1999

곤충병원선충(Steinernema carpocapsae Weiser) 기주 증식을 위해 실내 대량사육이 용이한 파밤나방(spodoptera exigua (Hubner))과 담배거세미나방(Sp. litura (Fabricius))유충에서 병원성과 증식선충수를 조사하였다. 반수치사 병원선충수는 두 기주간 또는 발육시기에 따라 차이가 없었으나 기주내에 증식된 선충수는 기주의 크기에 비례하였다. 온도에 따른 선충의 증식은 온도가 증가함에 따라 기주내 최대 감염태 선충수(담배거세미나방 5령충에서 약 500,000마리, 파밤나방 5령충에서 약 100,000 마리)를 보이는 소요기간이 $25^{\circ}C$에는 접종 후 6일이나 $20^{\circ}C$에서는 12일로 길어졌다.

• Pediatric Infection and Vaccine
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• 제23권3호
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• pp.229-235
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• 2016

최근 전 세계적으로 메티실린내성 황색포도알균(methicillin-resistant Staphylococcus aureus [MRSA])에 의한 감염성 심내막염의 빈도가 증가하는 추세이다. 저자들은 12세 여아가 침습적 치과 치료 이후에 발생한 MRSA에 의한 감염성 심내막염 1예를 경험하였기에 보고하는 바이다. 환아가 경부강직을 나타내어 시행한 뇌척수액 검사상 세포 증가증 소견이 보였고 세균성 수막염을 의심하여 항생제 치료를 시작하였으며, 입원 3병일째 혈액 배양검사에서 MRSA가 검출되었다. 심장 초음파상 승모판막의 전엽에 붙어있는 증식조직이 증명되어 항생제 치료 후 호전되어 퇴원하였다. 개정된 심내막염 예방을 위한 가이드라인에서는 오직 고위험군에서만 치과 처치 전에 예방적 항생제 요법을 권장하고 있다. 본 증례는 승모판 탈출증 이외에 고위험군이 없었던 환아에서 잇몸의 출혈을 유발하는 치과 치료를 받은 이후에 발병한 감염성 심내막염의 경우로서, 이후 12개월간 감염성 심내막염의 재발은 없었다.

• The Plant Pathology Journal
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• 제34권4호
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• pp.297-307
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• 2018

The sugar beet cyst nematode, Heterodera schachtii is a well known pathogen on Chinese cabbage in the highland fields of Korea. However, a race of cyst forming nematode with close morphological resemblance to H. trifolii was recently isolated from the same Chinese cabbage fields. Morphological species differentiation between the two cyst nematodes is challenging, with only minor differences between them. Thus, this study described the newly intercepted H. trifolii population, and reviewed morphological and molecular characteristics conceivably essential in differentiating the two nematode species. A comparison of morphometric measurements of both infective juveniles and vulval cones of cysts showed significant differences between the two cyst nematodes. When total RNA and genomic DNA were extracted from a mixed field population, COI genes and ITS regions were clearly amplified with primers of the two Heterodera species, suggesting that Heterodera population collected from the Chinese cabbage field consisted of a mixture of two species. COI and ITS of H. trifolii were predominantly amplified from nucleotides prepared from H. trifolii monoxenic population whereas those of H. schachtii were strongly detected in H. schachtii monoxenic cultures. Thus, this study confirms the coexistence of the two species in some Chinese cabbage fields; and the presence of H. trifolii in Korea is reported here for the first time.

• Journal of Preventive Medicine and Public Health
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• 제48권1호
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• pp.10-17
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• 2015

Objectives: An outbreak of acute febrile illness occurred in the Republic of Korea Air Force boot camp from May to July 2011. An epidemiological investigation of the causative agent, which was of a highly infective nature, was conducted. Methods: Throat swabs were carried out and a multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) assay was performed to identify possible causative factors. Results: The mean age of patients who had febrile illness during the study period was 20.24 years. The multiplex RT-PCR assay identified respiratory syncytial virus (RSV) as the causative agent. The main symptoms were sore throat (76.0%), sputum (72.8%), cough (72.1%), tonsillar hypertrophy (67.9%), and rhinorrhea (55.9%). The mean temperature was $38.75^{\circ}C$ and the attack rate among the recruits was 15.7% (588 out of 3750 recruits), while the mean duration of fever was 2.3 days. The prognosis was generally favorable with supportive care but recurrent fever occurred in 10.1% of the patients within a month. Conclusions: This is the first epidemiological study of an RSV outbreak that developed in a healthy young adult group. In the event of an outbreak of an acute febrile illness of a highly infective nature in facilities used by a young adult group, RSV should be considered among the possible causative agents.

• Journal of Chest Surgery
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• 제29권2호
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• pp.157-163
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• 1996

1986년 1월 부터 1994년 7월까지 원광대학병원 흉부외과학교실에서는 23명의 감염성 심내막염 환자를 수술하였다. 남녀의 비는 13:5 이 었고 평균 43.7세 이었다. 분포는 고유판막 심내막염 21례, 인공판막 심내막염 2례이었고 승모판막을 치환한 경우가 16례로 가장 많았고 대동맥 판막 치환술 11례, 삼천판막 치환술 2례 순이었다. 가장 흔한 감염균주는 연쇄상포도구균 이었으나 배양이 않된 경우는 7례나 되었다. 수술대상은 조절되지 않는 심부전증, 거대식균 그리고 판막부전을 보이는 경우이었고, 술후 사망율은 13.4%로 만기사망은 없었다. 사망의 주 원인은 뇌경색증과 저심박출증이었다. 술전 기능등급이 III-IV 이었던 모든 환자는 사망 3례를 제외한 모든 례에서 I-II 등급으로 호전되었다. 결론적으로 조기 수술적 조작은 지속적이거나 진행성 심부전을 갖고있는 환자에서는 감염의 활동성 내지는 항생제 치료의 유무에 관계없이 생명을구할수 있는 방법이다.

• Journal of Chest Surgery
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• 제46권4호
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• pp.256-264
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• 2013

Background: Surgical treatment of infective endocarditis (IE) remains a challenge, especially in cases of multiple valve surgery. We evaluated the clinical outcomes of native valve IE and compared the outcomes of single valve surgery with those of multiple valve surgery. Materials and Methods: From 1997 to 2011, 90 patients underwent surgery for native valve IE; 67 patients with single valve surgery (single valve group) and 23 patients with multiple valve surgery (multiple valve group). The mean follow-up duration was $73.1{\pm}47.4$ months. Results: The surgical mortality in the total cohort was 4.4%. The overall survival (p=0.913) and valve-related event-free survival (p=0.204) did not differ between the two groups. The independent predictor of postoperative complications was New York Heart Association class (p=0.001). Multiple valve surgery was not a significant predictor of surgical mortality (p=0.225) or late mortality (p=0.936). Uncontrolled infection, urgent or emergency surgery, and postoperative complications were identified as independent predictors of valve-related morbidity, excluding multiple valve surgery (p=0.072). Conclusion: In native valve IE, multiple valve surgery as a factor was not an independent predictor of mortality and morbidity. The number of surgically corrected valves in native IE seems to be unrelated to perioperative and long-term outcomes.

• International Journal of Oral Biology
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• 제30권4호
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• pp.125-130
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• 2005

Thrombin-induced platelet microbicidal proteins (tPMP) are antibacterial proteins released when platelets are stimulated by thrombin. It has been reported that tPMP has antibacterial activity against various bacterial species including causative agents of infective endocarditis. Most of the oral streptococci have resistance to the killing by tPMP and this fact may play an important role as a virulence factor in infective endocarditis. However, the susceptibility and resistance mechanism of oral streptococci for tPMP have not been revealed yet. In this study, the killing mechanism of tPMP for oral streptococci has been investigated. Streptococcus rattus BHT, a susceptible strain, and Streptococcus gordonii DL1, a resistant strain, have been used in this study. tPMP was isolated from platelet after stimulation with thrombin. Cell membrane depolarization was examined with 3,3'-dipropylthiodicarbocyanine iodide ($DiSC_3$), membrane potential-sensitive cyanine dye, by fluorescence spectrophotometry. The permeabilization of cell membrane by tPMP was investigated with propidium iodide (PI) by flow cytometry. tPMP susceptible S. rattus BHT showed the increase of the $DiSC_3$ fluorescence level meaning depolarization of cell membrane and increase of the uptake of PI which means permeabilization of cell membrane. However, tPMP resistant S. gordonii DLI did not show depolarization and permeabilization. These results indicate that the increasing depolarization and permeabilization of oral streptococcal cell membrane are associated with the bactericidal activity of tPMP.

• 한국미생물·생명공학회지
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• 제29권2호
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• pp.104-109
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• 2001

공생 박테리아 Photorhabdus sp. strain TF 균체량이 증가함에 따라서 병원성 선충 Heterorhabditis bacteriophora의 Infective Juveniles의 생산이 증가되었다. 이 공생 박테리아의 성장 최적 조건은 각각 배양 온도 3$0^{\circ}C$ 그리고 초기 배지 pH 5.5부터 7.3 사이에서 얻어졌다. 통기 상태에서 박테리아 균체의 생산과 성장이 촉진됨이 밝혀졌다. Photorhabdus sp. strain TF 박테리아의 성장 중 생산된 색소와 배양액의 pH의 변화는 박테리아의 성장 정도 나타내어 액체배양에서 병원성 선충 Heterorhabditis bacteriophora의 접종시기로 표시로서 이용할 수 있을 것이다.

• Parasites, Hosts and Diseases
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• 제45권2호
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• pp.121-128
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• 2007

Arthrostoma miyazakiense (Nematoda: Ancylostomatidae) is a hookworm species reported from the small intestines of raccoon dogs (Nyctereutes procyonoides) in Japan. Five Korean raccoon dogs (N. procyonoides koreensis) caught from 2002 to 2005 in Jeollanam-do (Province), a southeastern area of South Korea, contained helminth eggs belonging to 4 genera (roundworm, hookworm, whipworm, and Capillaria spp.) and cysts of Giardia sp. in their feces. Necropsy findings of 1 raccoon dog revealed a large number of adult hookworms in the duodenum. These hookworms were identified as Arthrostoma miyazakiense based on the 10 articulated plates observed in the buccal capsule and the presence of right-sided prevulval papillae. Eggs of A. miyazakiense were $60-65{\times}35-40{\mu}m$ (av, $62.5{\times}35{\mu}m$), and were morphologically indistinguishable from those of Ancyiostoma caninum. The eggs were cultured to infective 2nd stage larvae via charcoal culture, and 100 infective larvae were used to experimentally infect each of 3 mixed-bred puppies. All puppies harbored hookworm eggs in their feces on the 12th day after infection. This is the first report thus far concerning A. miyazakiense infections in raccoon dogs in Korea, and the first such report outside of Japan.

• 대한족부족관절학회지
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• 제26권2호
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• pp.66-70
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• 2022

Purpose: A group of patients who were hospitalized for diabetic foot ulcers was classified according to the University of Texas Staging System for Diabetic Foot Ulcers, and we attempted to evaluate whether this staging system could be a criterion for treatment success using vacuum-assisted closure (VAC) technique. Materials and Methods: A total of 32 patients were diagnosed with diabetic foot ulcers according to the University of Texas Staging System for Diabetic Foot Ulcers. Of these, 24 patients who were evaluated as stage B according to the staging system were classified as Group 1, and 8 patients in stage D were classified as Group 2. After applying VAC, the treatment success rate was compared by evaluating the size and severity of ulcers between the two groups. Results: The grade of granulation after VAC was on average 3.75±0.53 in Group 1 and 2.25±0.71 in Group 2. There was better granulation after VAC application in Group 1 (p<0.01). The success rate of the treatment was 22 cases (91.67%) in Group 1 and one case (12.5%) in Group 2. Thus there were statistically significant differences in the success rate of treatment between groups 1 and 2 (Pearson's chisquare test, p=0.01; odd ratio 77.00, 95% confidence interval [CI] 1.26~14.66; relative risk 4.30, 95% CI 1.26~14.66). Conclusion: These results suggest that there was a higher success rate of treatment with VAC in stage B patients. The University of Texas Staging System for Diabetic Foot Ulcers can thus be an index for applying VAC to patients with infective diabetic foot ulcers.