• Journal of the Korean GEO-environmental Society
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• v.15 no.4
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• pp.5-11
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• 2014

Recently, there have been various lifeline installations constructed in the underground space of urban area due to the effective use of land. For newly installed lifelines or the management of the installed lifelines, many construction activities of excavation and backfilling are observed. Around these area, there are possibilities of collapse or excessive settlement due to the leaking of the pipe or unsatisfactory compaction of backfill material. Besides, construction costs can be saved since the on-site soils are used. The application of this liquidity filling material is not only to the lifeline installation but also to underpin the foundation under the vibrating machinery. On the evaluation of the applicability of this method to this circumstance, the strength should be investigated against the static load from the machine load as well as the vibration load from the activation of the machine. In this study, the applicability of the liquidity fill material on the foundation under the vibrating machinery is assessed via uniaxial compression and resonant column tests. The liquidity filling material consisting of the on-site soils with loess and kaolinite are tested to investigate the static and dynamic characteristics. Furthermore, the applicability of the reclaimed ash categorized as an industrial waste is evaluated for the recycle of the waste to the construction materials. The experimental results show that the shear modulus and 7 day uniaxial strength of the liquidity filling material mixed with reclaimed ash show higher than those with the on-site soils. However, the damping ratio does not show any tendency on the mixed materials.

• Korean Journal of Ecology and Environment
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• v.33 no.2 s.90
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• pp.109-118
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• 2000

The dynamics of inorganic nutrients and phytoplankton population were examined at eight stations of Shihwa Reservoir, which situated near the cities newly constructed and the industrial complex of West-sea in Korea, from January to December 1999. Among environmental factors, average concentration of $NH_4$, SRP and SRSi were $522.7\;{\mu}g\;N/l$, $9.8\;{\mu}g\;N/l$ and $0.26\;{\mu}g\;Si/l$, respectively. Water quality was extremely deteriorated by a great amount of pollutants load into inner reservoir after the event of rainfall. Nutrients concentration was suddenly decreased toward the lower part. While $NO_3$ concentration did not much varied among stations, but it was relatively high in winter season. Chlorophyll-a concentration was high at the upper part of the reservoir, with average of $37.2\;{\mu}/l$, and closely related to the fluctuation of $NH_4$, SRP and SRSi concentrations. The phytoplankton development in the water column was dominated by diatom (autumn), prasinoid (winter) and dinoflagellate (summer). Dominant phytoplankton were composed to Skeletonema costatum of diatom, Prorocentrum minimum of dinoflagellate, Chroomonas spp. of cryptomonad, Eutreptiella gymnastica of euglenoid and Pyramimonas spp. of prasinoid. The large bloom of phytoplankton at the upper zone of the Shihwa Reservoir after inflow of a seawater were consistently observed. In consequence, water quality management of the inlet stream was assessed to be very important and urgent.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.89-119
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• 2004

This study was conducted to isolate lactobacilli having probiotic characteristics to be used as health adjuncts with fermented milk products. Acid tolerant strains were selected in Lactobacilli MRS broth adjusted to pH 4.0 from 80 healthy persons (infants, children and adults). And bile tolerant strains were examined in Lactobacilli MRS broth in which 1.0% bile salt was added. By estimation above characteristics, the strains No. 27, which was isolated from adult feces, was selected and identified as Lactobacillus salivarius subsp. salivarius based on carbohydrate fermentation and 16S rDNA sequencing. It was used as a probiotic strain in fermented milk products. The pH of fermented milk decreased from pH 6.7 to 5.0 and titratable acidity increased from 0.3% to 1.0% by L. salivarius subsp. salivarius (isolation strain 20, 35, and 37), when incubated for 36 h at $37^{\circ}C$. The number of viable cell counts of fermented milk was maximized at this incubation condition. The SDS-PAGE evidenced no significant change of casein but distinct changes of whey protein were observed by isolated L. salivarius subsp. salivarius for titratable acidity being incubated by $0.9{\sim}1.0%$ at $37^{\circ}C$. All of the strains produced 83.43 to 131.96 mM of lactic acid and 5.39 to 26.85 mM of isobutyric acid in fermented products. The in vitro culture experiment was performed to evaluate ability to reduce cholesterol levels and antimicrobial activity in the growth medium. The selected L. salivarius subsp. salivarius reduced $23{\sim}38%$ of cholesterol content in lactobacilli MRS broth during bacterial growth for 24 hours at $37^{\circ}C$. All of the isolated L. salivarius subsp. salivarius had an excellent antibacterial activity with $15{\sim}25$ mm of inhibition zone to E. coli KCTC1039, S. enteritidis KCCM3313, S. typhimurium M-15, and S. typhimurium KCCM40253 when its pH had not been adjusted. Also, all of the isolated L. salivarius subsp. salivarius had partial inhibition zone to E. coli KCTC1039, E. coli KCTC0115 and S. enteritidis KCCM3313 when it had been adjusted to pH 5.7. The selected strains were determined to have resistances of twelve antibiotic. Strains 27 and 35 among the L. salivarius subsp. salivarius showed the highest resistance to the antibiotics. Purified ${\alpha}$-galactosidase was obtained by DEAE-Sephadex A-50 ion exchange chromatography, Mono-Q ion exchange chromatography and HPLC column chromatography from L. salivarius subsp. salivarius 27. The specific activity of the purified enzyme was 8,994 units/mg protein, representing an 17.09 folds purification of the original cell crude extract. The molecular weight of enzyme was identified about 53,000 dalton by 12% SDS-PAGE. Optimal temperature and pH for activity of this enzyme were $40^{\circ}C$ and 7.0 respectively. The enzyme was found to be stable between 25 and $50^{\circ}C$. ${\alpha}$-galactosidase activity was lost rapidly below pH 5.0 and above pH 9.0. This enzyme was liberated galactose from melibiose, raffinose, and stachyose, and also the hydrolysis rate of substrate was compound by HPLC. These results indicated that some of the L. salivarius subsp. salivarius (strain 27 and 35) are considered as effective probiotic strains with a potential for industrial applications, but the further study is needed to establish their use as probiotics in vivo.

• Journal of the Korean Chemical Society
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• v.39 no.9
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• pp.715-721
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• 1995

Reaction between trans-$[Co(py)_4/Ci_2]^+(py=pyridine)$ and L-proline and diimine (=2,2'-bipyridine, 1,10-phenanthroline) gives two products, $[Co(L-pro)_2/(bipy)]^+$ and $[Co(L-pro)_2(phen)]^+$ complexes, respectively. On column chromatography, $[Co(L-pro)_2(bipy)]^+$ was obtained only as $Lambda$-trans(N) and $[Co(L-pro)_2(phen)]^+$ was obtained both as ${\Delta}$-trans(N) and $Lambda$-cis(O)cis(N) due to the stereoselectivity of L-prolinato which was stereospecific. Crystal data are as follows: $Lambda$-trans(N)-$[Co(L-pro)_2(bipy)]CIO_4{\cdot}2H_2O$ (1): monoclinic, space group $P2_1(#4)$, a=9.807(3), b=10.421(1), c=12.778(2) ${\AA}$, ${\beta}=109.90(2)^{\circ}$, V=1227.8(5) ${\AA}^3$, Z=2; 1571 data with I > 3.0${\sigma}$(I) were refined to R=0.060, $R_W = 0.067$; ${\Delta}$-trans(N)-$[Co(L-pro)_2(phen)]Cl{\cdot}_3H_2O$(2): monoclinic, space group $P2_1(#4)$, a=9.838(2), b=12.892(2), c=10.747(2)${\AA}$, ${\beta}=113.79(2)^{\circ}$, V=1247.2(4) ${\AA}^3$, Z=2; 2433 data with I > 3.0${\sigma}$(I) were refined to R=0.043, $R_W = 0.050$.

• Journal of agriculture & life science
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• v.50 no.1
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• pp.273-281
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• 2016

In this study, after carrying out a bending test that targeted the frames of plastic film greenhouse, the load-displacement relationship was analyzed to be used as basic data to develop greenhouse construction and maintenance guidelines. As a result, regardless of the shapes of the specimen, the yield and the maximum load increased as the size of the specimen increased. The displacement also showed the same pattern. A steel pipe showed lower yield and maximum load than a square pipe, and the displacement was large. In the steel pipe case, the displacement under the yield and maximum load was in the range of approximately 1.42-4.20mm and 5.80-24.13mm, respectively. In the square pipe case, the displacement under the yield and maximum load was in the range of approximately 1.62-3.00mm and 3.13-8.01mm, respectively. Further, a large difference was observed between the result of this test and the values calculated by a conventionally provided standard. In particular, not much difference was found from the result of this test in the case of a purlin member from the values provided by previous researches. However, a large difference was observed in the column or main rafter members. Furthermore, when a wide-span and venlo type, which is a glasshouse, was used as a target(h/100 and h/80), the displacement under the yield and maximum load was approximately 28.0mm and 35.0mm, respectively, which showed a large difference compared with the Netherlands standard(14.0mm) of a glasshouse. Further, in the main rafter case, a large difference was observed in the displacement limit according to the width(i.e., span) of the greenhouse where members are used. Therefore, because the displacement limit can vary depending on various factors such as type, form, and size of a greenhouse, we determined that studies or tests that consider these factors should be carried out to reflect them in the construction and maintenance of greenhouses.

• Applied Biological Chemistry
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• v.15 no.2
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• pp.93-109
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• 1972

Three lots of Chung-Kook-Jang were prepared by the use of 2 strains of Bacillus subtilis and Bacillus natto. For four samples taken from each lot in 12 hrs interval changes of nitrogenous compounds, insoluble protein, water soluble protein, peptides, free amino acids, amino and ammonia nitrogens during Chung-Kook-Jang fermentation, were studied together with the changes of moisture, pH, proteolytic enzyme activity. In addition the average peptide length of the peptides of a Bacillus subtilis lot was determined by the method of molecular sieving using ion exchange resin. The results were as follows: 1. The contents of moisture and total-nitrogen changed little in all samples throughout the fermentation as it would be expected. 2. In all three experimental lots the pH became higher gradually from the initial value of 6.65 to the final $7.5{\sim}7.85$ during the fermentation. Proteolytic enzyme activities, in accordance with this pH change, steadily increased up to $48{\sim}60$ hrs. of fermentation and then slightly decreased, probably affected by the high pH. The most strong proteolytic activity was observed in the experimental Chung-Kook-Jang fermentation lot using the Bacillus subtilis K-27 isolated by the author. 3. The contents of insoluble protein nitrogen in soybeans increased markedly (5%) by the cooking, after steeping 12 hrs in water. During the Chung-Kook-Jang fermentation, however, it decreased from 1/2 to 1/10 of that of the cooked soybeans. 4. The contents of water soluble protein nitrogen (5%) whereas, greatly decreased to the value of 1.0% by the cooking; but little changed further during the fermentation, 5. The total contents (0.25%) of peptides, amino, and ammonia-nitrogens, PAA-N., increased almost double by the cooking and steadily became higher as the fermentation proceeded, reaching finally up to$4{\sim}7%$ in 72 hrs fermentation. 6. The amounts of free amino acids of soybean generally decreased during the processing of cooking, even some of them like glutamic acid were destroyed completely, However in the subsequent Chung-Kook-Jang fermentation for 72 hrs., they showed from several to a few hundreds folds increases depending upon the kinds of amino acids. Valine which was contained in HCl-hydrolyzed steeped or cooked soybeans in amounts $220{\sim}267mg%$ was not detected at all as the free amino acid in all fermented samples. 7. Average peptide length (APL) of all fractions, eluted and fractionated by using the Dowex-50 ion exchange resin column, and fraction collector showed the highest value for the cooked soybean and then decreased as the fermentation proceeded. The APL value of effluent showed the highest in 12 hrs fermented sample, The value decreased thereafter by fermentation.