• 생명과학회지
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• 제16권1호
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• pp.30-36
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• 2006

호중구의 세포사멸은 자연적으로 일어나지만 여러 외부자극에 의한 신호의 전달에 의하여 증가하거나 지연된다. 본 연구에서는 항암, 항생제로 개발된 프레닐 페놀계인 ascochlorin의 유도체 중에서 백혈구 암의 세포사멸을 유도하는 4-O-methyl-ascochlorin (MAC)이 호중구의 자연 세포사멸 및 지연되는 세포사멸에 어떠한 영향을 미치는가와 그 작용기작을 연구하였다. 호중구의 세포사멸은 사람 말초 혈액으로부터 분리하여 세포 배양 시간에 따라 형태 변화, annexin-V/propidium iodide의 염색, 및 DNA 전기영동 등으로 조사하였다. MAC는 농도 및 시간 의존 형으로 호중구의 세포사멸을 증가시켰다. 그러나 granulocyte macrophage-colony stimulating factor나 lipopolysaccharide 등에 의한 세포사멸의 지연은 MAC에 의하여 부분적으로 억제되었다. MAC에 의한 세포사멸의 유도는 pancaspase, caspase-8 및 caspase-3 억제제인 zVAD-fmk. zIETD-fmk, 및 zDEVD-fmk에 의하여 억제되었으며 procaspase-8과 procaspase-3의 단백질 양도 MAC로 처리한 호중구에서 현저히 감소하였다. 미토콘드리아 막 투과성은 MAC에 의하여 현저히 감소하였으나 zVAD-fmk에 의하여 완전히 봉쇄되지 못하였다. 이들 결과 들은 MAC에 의한 호중구 세포사멸의 증가는 caspase-8 및 caspase-3의 활성을 통하여 일어나지만 미토콘드리아의 막성분에는 영향이 없다는 것을 제시하고 있다.

• 동의생리병리학회지
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• 제25권2호
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• pp.195-201
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• 2011

Nardostachys chinensis;Anti-proliferation;Cell cycle arrest;Differentiation;U937 cells; This study was conducted to determine the analgesic effect of Sokyungwhalhyul-tang(SKWHT) using the model of peripheral neuropathic pain model. A model of neuropathic pain was made by ligating left 5th lumbar spinal nerve of rats. After 1 days, the extract of SKWHT was orally administered daily. Rats were divided into four groups; (1) Control group(n=6), (2) Experimental group I(SKWHT-OA1, 100 mg/kg, n=6), (3) Experimental group II(SKWHT-OA2, 300 mg/kg, n=6), (4) Experimental group III(SKWHT-OA3, 500 mg/kg, n=6). After that, we examined the withdrawl response of neuropathic rats legs by von Frey filament and Hot plate at pre, $1^{th}$, $4^{th}$, $7^{th}$, $14^{th}$, $21^{th}$ days after the induction of neuropathic pain. And also we examined c-fos, GOT, GPT and histological study of Liver at 21th days. von Frey filament and Hot plate were increase in experimental group I, II, III than Con. especially group III was most significantly analgesic effect than the other groups at $14^{th}$, $21^{th}$ days. In c-fos protein expression on spinal cord, group III was most significantly reduction immunoreactivity at $21^{th}$ days and in blood serum GOT & GPT levels and histologic finding of Liver in all experimental groups were no significant difference with Con at $21^{th}$ days. According to the above results, SKWHT(500 mg/kg) may have a significant analgesic effect on the neuropathic pain.

• 국제물리치료학회지
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• 제1권1호
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• pp.10-18
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• 2010

This study was performed to investigate the effects of Needle Electrode Electrical Stimulation(NEES) on ischemia-induced cerebrovascular accidents. After obstruction and reperfusion of arteries in white mice, the amounts of necrosis and inflammation related substances Bax, IL-6, Caspase-3, and COX-2 were measured in neurons of the fore-brain. The following results were obtained. This study used 21 male specific pathogen free(SPF) SD rats, 8 weeks of age and approximately 300g in weight. Each exposed artery was completely occluded with non-absorbent suture thread and kept in that state for 5 minutes. The sutures were then removed to allow reperfusion of blood. Test group is control group(common carotid artery occlusion models), a GI(underwent common carotid artery occlusion), and NEES(underwent NEES after artery occlusion). The GI and NEES groups were given 12, 24, or 48 hours of reperfusion before NEES. NEES device(PG6, ITO, Japan, 9V, current, 2Hz) was used to stimulate the bilateral acupoint ST36 of the SD rats for 30 minutes while they were sedated with 3% isoflurane. An immuno-histochemistry test was done on the forebrains of the GI induced rats. Both Bax and Caspase-3 immuno-reactive cells, related to apoptosis, were greater in the GI than the NEES group. Cox-2 and IL-6 immuno-reactive cells, related to inflammation, were greater in the GI and NEES groups than the control group. We can expect that applying NEES after ischemic CVA is effective for preventing brain cells from being destroyed. And we can conclude NEES should be applyed on early stage of ischemic CVA.

• 대한의생명과학회지
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• 제23권2호
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• pp.49-56
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• 2017

Fibroblast growth factor (FGF)-2 is one of the most effective growth factors to increase the growth rate of mesenchymal stem cells (MSCs). Previously, we reported that low dose of FGF-2 (1 ng/ml) induced proliferation of bone marrow-derived mesenchymal stem cells (BMSCs) through AKT and ERK activation resulting in reduction of autophagy and senescence, but not at a high dose. In this study, we investigated the effects of high dose FGF-2 (10 ng/ml) on proliferation, autophagy and senescence of BMSCs for long term cultures (i.e., 2 months). FGF-2 increased the growth rate of BMSCs in a dose dependent manner for a short term (3 days), while during long term cultures (2 months), population doubling time was increased and accumulated cell number was lower than control in BMSCs when cultured with 10 ng/ml of FGF-2. 10 ng/ml of FGF-2 induced immediate de-phosphorylation of ERK1/2, expression of LC3-II, and increase of senescence associated ${\beta}$-galactosidase (SA-${\beta}$-Gal, senescence marker) expression. In conclusion, we showed that 10 ng/ml of FGF-2 was inadequate for ex vivo expansion of BMSCs because 10 ng/ml of FGF-2 induced growth retardation via ERK1/2 de-phosphorylation and induction of autophagy and senescence in BMSCs.

• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.524-528
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• 2001

The measurement of radiation response using simple and informative techniques would be of great value in studying the genetic risk following occupational, therapeutic, or accidental exposure to radiation. When patients receive radiation therapy, many suffer from side effects. Since each patient receives a different dose due to different physical conditions, it is important to measure the exact dose of radiation received by each patient to lessen the side effects. Even though several biological dosimetric systems have already been developed, there is no ideal system that can satisfy all the criteria for an idean dosimetric system, especially for low-dose radiation as used in radiation therapy. In this study, an SOS Chromotest of E. coli PQ37 was evaluated as a novel dosimeter for low-dose gamma-rays. E. coli PQ37 was originally developed to screen chemical mutagens using the SOS Chromotest-a colorimtric assay, based on the induction of ${\beta}$-galactosidase ue to DNA damage. The survival fraction of E. coli PQ37 decreased dose-dependently with an increasing dose of cobalt-60 gamma-rays. Also, a good linear correlation was found between the biological damage revealed by the ${\beta}$-galactosidase expression and the doses of gamma-rays. The expression of ${\beta}$-galactosidase activity that responded to low-dose radiation under 1 Gy was $Y=0.404+(0.089{\pm}0.3)D+(-0.018{\pm}0.16)D^2$ (Y, absorbance at 420 nm; D, Dose of irradiation) as calculated using Graph Pad In Plot and Excel. When a rabbit was fed with capsules containing an agar block embdded with E. coli PQ37 showed a linear response to the radiation doses. Accordingly, the results confirm that E. coli PQ37 can be used as a sensitive biological dosimeter fro cobalt-60 gamma-rays. To the best of our knowledge, this is the first time that a bacterium has been used as a biological dosimeter, especially for low-dose radiation.

• Tuberculosis and Respiratory Diseases
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• 제41권5호
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• pp.546-551
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• 1994

금치료는 류마티스 관절염에서 고식적인 치료에 반응하지 않는 경우 유용하게 사용되고 있고, 드물게 금제에 의한 과민성 폐렴을 유발하는 것으로 알려져 있다. 저자등은 6년간 혈청반응검사 양성 류마티스 관절염을 앓아 왔던 26세 여자환자에서 Gold sodium thiomalate 300 mg을 사용후 발생원 과민성 폐렴 1예를 림프구 자극 시험으로 확인하여 문헌고찰과 함께 보고한다.

• 한국융합학회논문지
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• 제9권10호
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• pp.141-147
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• 2018

본 연구의 목적은 유도된 발목 근피로 시 테이핑, 스트레칭 그리고 초음파가 융합적 균형능력에 미치는 효과를 알아보는 데 있다. 건강한 성인 남녀 39명이 본 연구에 자발적으로 참여하였다. 발목 근피로 유발 이후 대상자들은 테이핑, 정적 스트레칭 또는 초음파 그룹으로 무작위 선정되었다. 그리고 각 그룹에 해당하는 중재방법이 적용되었다. 균형 능력은 Space Balance 3D를 이용하여 발목 근피로 전과 후 그리고 중재 후에 측정되었다. 균형능력은 그룹 간 유의한 차이는 없었지만(p>.05), 그룹 내 모두에서 유의하게 증가하였다(p<.001). 그러므로 발목 근 피로에 테이핑, 정적 스트레칭 그리고 초음파는 융합적 균형능력에 효과적일 것이다. 향후 연구에서는 다양한 측정변수 측정과 치료적 중재를 적용하여 치료적 효과를 증명할 필요가 있다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제37권5호
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• pp.396-402
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• 2011

Introduction: Epidermal growth factor is a single-chain polypeptide consisting of 53 amino acids with potent mitogenic activity that stimulates the proliferation of a range of normal and neoplastic cells through an interaction with its specific receptor (epidermal growth factor receptor, EGFR). This interaction plays a key role in tumor progression including the induction of tumor cell proliferation. An increased EGFR copy number have been associated with a favorable response to EGFR tyrosine kinase inhibitors therapy. In contrast, K-ras mutations tend to predict a poor response to such therapy. The aim of this study was to determine the correlation between the clinicopathological factors and the up-regulation of EGFR expression and Kras mutations in oral squamous cell carcinoma. Materials and Methods: This study examined the immunohistochemical staining of EGFR, K-ras mutation detection with peptide nucleic acid (PNA)-based real-time polymerase chain reaction (PCR) clamping in 20 specimens from 20 patients with oral squamous cell carcinoma. Results: 1. In the immunohistochemical study of poorly differentiated and invasive oral squamous cell carcinoma, a high level of EGFR staining was observed. The correlation between immunohistochemical EGFR expression and histological differentiation, as well as the tumor size of the specimens was significant (Pearson correlation analysis, significance [r] >0.5, P<0.05). 2. In PNA-based real-time PCR clamping analysis, a K-ras mutation was not detected in all specimens. Conclusion: These findings suggest that the up-regulation of the EGFR may play a role in the progression and invasion of oral squamous cell carcinoma that is, independent of a K-ras mutation.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제34권1호
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• pp.59-70
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• 2008

Purpose: We evaluated the therapeutic effect of AEE788, a dual inhibitor of epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) receptor tyrosine kinases on human salivary adenoid cystic carcinoma (ACC) cells growing in nude mice. Experimental Design: We examined the effects of AEE788 on salivary ACC cell growth and apoptosis. To determine the in vivo effects of AEE788, nude mice with orthotopic parotid tumors were randomized to receive oral AEE788 (50 mg/kg) three times per week, injected paclitaxel ($200{\mu}g$) once per week, AEE788 plus paclitaxel, or placebo. Mechanisms of in vivo AEE788 activity were determined by immunohistochemical analysis. Results: Treatment of salivary ACC cells with AEE788 led to growth inhibition and induction of apoptosis. AEE788 inhibited tumor growth and prevented lung metastasis in nude mice. Furthermore, AEE788 potentiated growth inhibition and apoptosis of ACC tumor cells mediated by paclitaxel. Tumors of mice treated with AEE788 and AEE788 plus paclitaxel exhibited down-regulation of activated EGFR and its downstream mediators (Akt and MAPK), increased tumor and endothelial cell apoptosis, and decreased microvessel den-sity, which correlated with a decrease in the level of MMP-9, MMP-2 and bFGF expression and a decrease in the incidence of vascular metastasis. Conclusions: These data show that tumor-associated endothelial cells are important in the process of tumor-metastasis. And VEGFR can be a molecular target for therapy of metastatic lung lesion of salivary ACC.

• 한국컴퓨터정보학회논문지
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• 제25권8호
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• pp.145-150
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• 2020

전방머리자세는 머리와 목의 잘못된 자세 정렬 중 하나로 비정상적인 관절 위치감각과 고유수용성 감각손상으로 인해 목의 통증과 균형 손상까지 발생시키는 주요 원인으로 알려져 있다. 키네시오 테이핑은 통증관리를 위해 사용되는 임상적인 중재방법으로 통증감소, 혈액순환 촉진, 근육이완을 유도하여 관절 위치 교정 및 근육과 관절에 안정성을 제공하는 효과를 가지고 있다. 이전의 많은 연구에서 키네시오 테이핑을 활용하여 목 통증감소와 목의 정렬에 긍정적인 효과가 있음을 입증하였지만 일부 연구에서는 통증 완화의 효과를 입증하지 못한 부분도 있다. 키네시오 테이핑 적용 후 즉각적인 통증완화 및 정렬 개선에 효과를 보이긴 하지만 적용이후 효과의 지속에 대해서는 증거가 충분하지 않고 임상적으로 더 큰 가치를 보이기 위해서는 키네시오 테이핑의 장점에 대해서 추가적인 연구를 권장하였다. 따라서 본 연구는 앞머리 자세를 가진 사람에서 키네시오 테이핑이 머리 자세의 정렬과 동적 균형 능력에 미치는 영향을 조사하고자 한다.