• Biomolecules & Therapeutics
• /
• 제32권4호
• /
• pp.451-459
• /
• 2024

Apoptosis signal-regulating kinase 1 (ASK1) is an upstream signaling molecule in oxidative stress-induced responses. Because oxidative stress is involved in asthma pathogenesis, ASK1 gene deficiency was investigated in animal models of allergic asthma. However, there is no study to investigate whether ASK1 inhibitors could be applied for asthma to date. Selonsertib, a potent and selective ASK1 inhibitor, was applied to BALB/c mice of an ovalbumin (OVA)-induced allergic asthma model. Selonsertib suppressed antigen-induced degranulation of RBL-2H3 mast cells in a concentration-dependent manner. The administration of selonsertib both before OVA sensitization and OVA challenge significantly reduced airway hyperresponsiveness, and suppressed eosinophil numbers and inflammatory cytokine levels in the bronchoalveolar lavage fluid. Histopathologic examination elucidated less inflammatory responses and reduced mucin-producing cells around the peribronchial regions of the lungs. Selonsertib also suppressed the IgE levels in serum and the protein levels of IL-13 in the bronchoalveolar lavage fluid. These results suggest that selonsertib may ameliorate allergic asthma by suppressing immune responses and be applicable to allergic asthma.

• Journal of electromagnetic engineering and science
• /
• 제4권3호
• /
• pp.119-123
• /
• 2004

This paper presents here the results of the measurements of rain-induced attenuation in the vertically polarized signal propagating at 12.2525 GHz during some rain events, which occurred in the rainy wet season of the year 2001 at Yong-in, Korea(temperate climate). The attenuation measured experimentally was compared with that obtained using the International Telecommunication Union Radio Communication Sector(ITU-R) model, the SAM model and the Global model. In this paper, measured results are in good agreement with the ITU-R prediction.

• BMB Reports
• /
• 제36권5호
• /
• pp.468-474
• /
• 2003

The molecular mechanism of hypoxia-induced apoptosis has not been clearly elucidated. In this study, we investigated the involvement of extracellular signal-regulated protein kinase (ERK 1/2) in hypoxia-induced apoptosis using cobalt chloride in HeLa human cervical cancer cells. The cobalt chloride was used for the induction of hypoxia, and its $IC_{50}$ was $471.4\;{\mu}M$. We demonstrated the DNA fragmentation after incubation with concentrations more than $50\;{\mu}M$ cobalt chloride for 24 h, and also evidenced the morphological changes of the cells undergoing apoptosis with electron microscopy. Next, we examined the signaling pathway of cobalt chloride-induced apoptosis in HeLa cells. ERK1/2 activation occurred 6 and 9 h after treatment with $600\;{\mu}M$ cobalt chloride. Meanwhile, the pretreatment of the MEK 1 inhibitor (PD98059) completely blocked the cobalt chloride-induced ERK 1/2 activation. At the same time, the activated ERK 1/2 translocated into the nucleus and phosphorylated its transcriptional factor, c-Jun. In addition, the pretreatment of PD98059 inhibited the cobalt chloride-induced DNA fragmentation and apoptotic cell death. These results suggest that cobalt chloride is able to induce apoptotic activity in HeLa cells, and its apoptotic mechanism may be associated with signal transduction via ERK 1/2.

• The Korean Journal of Physiology and Pharmacology
• /
• 제24권3호
• /
• pp.203-212
• /
• 2020

Promyelocytic leukemia (PML) gene, through alternative splicing of its C-terminal region, generates several PML isoforms that interact with specific partners and perform distinct functions. The PML protein is a tumor suppressor that plays an important role by interacting with various proteins. Herein, we investigated the effect of the PML isoforms on oncostatin M (OSM)-induced signal transducer and activator of transcription-3 (STAT-3) transcriptional activity. PML influenced OSM-induced STAT-3 activity in a cell type-specific manner, which was dependent on the p53 status of the cells but regardless of PML isoform. Interestingly, overexpression of PML exerted opposite effects on OSM-induced STAT-3 activity in p53 wild-type and mutant cells. Specifically, overexpression of PML in the cell lines bearing wild-type p53 (NIH3T3 and U87-MG cells) decreased OSM-induced STAT-3 transcriptional activity, whereas overexpression of PML increased OSM-induced STAT-3 transcriptional activity in mutant p53-bearing cell lines (HEK293T and U251-MG cells). When wild-type p53 cells were co-transfected with PML-IV and R273H-p53 mutant, OSM-mediated STAT-3 transcriptional activity was significantly enhanced, compared to that of cells which were transfected with PML-IV alone; however, when cells bearing mutant p53 were co-transfected with PML-IV and wild-type p53, OSM-induced STAT-3 transcriptional activity was significantly decreased, compared to that of transfected cells with PML-IV alone. In conclusion, PML acts together with wild-type or mutant p53 and influences OSM-mediated STAT-3 activity in a negative or positive manner, resulting in the aberrant activation of STAT-3 in cancer cells bearing mutant p53 probably might occur through the interaction of mutant p53 with PML.

• 한국동물학회:뉴스레터
• /
• 제16권1호
• /
• pp.56-59
• /
• 1999

• The Korean Journal of Physiology and Pharmacology
• /
• 제19권2호
• /
• pp.89-97
• /
• 2015

The aim of this study was to investigate the involvement of dopaminergic receptors (DR) in behavioral sensitization, as measured by locomotor activity, and the over-expression of cocaine- and amphetamine-regulated transcript (CART) peptides after repeated administration of cocaine in mice. Repeated administrations of cocaine induced behavioral sensitization and CART over-expression in mice. The levels of striatal CART mRNA were significantly increased on the $3^{rd}$ day. CART peptides were over-expressed on the $5^{th}$ day in the striata of behaviorally sensitized mice. A higher proportion of $CART^+$ cells in the cocaine-treated mice were present in the nucleus accumbens (NAc) shell than in the dorsolateral (DL) part of caudate putamen (CP). The concomitant administration of both $D_1R$ and $D_2R$ antagonists, SCH 23390 ($D_1R$ selective) and raclopride ($D_2R$ selective), blocked cocaine induced-behavioral sensitization, CART over-expression, and cyclic adenosine 5'-monophosphate (cAMP)/ protein kinase A (PKA)/phospho-cAMP response element-binding protein (pCREB) signal pathways. SCH 23390 more predominantly inhibited the locomotor activity, CART over-expression, pCREB and PKA activity than raclopride. Cocaine induced-behavioral sensitization was also attenuated in the both $D_1R$ and $D_2R$ knockout (KO) mice, respectively. CART over-expression and activated cAMP/PKA/pCREB signal pathways were inhibited in the $D_1R$-KO mice, but not in the $D_2R$-KO mice. It is suggested that behavioral sensitization, CART over-expression and activated cAMP/PKA/pCREB signal pathways induced by repeated administration of cocaine could be more predominantly mediated by $D_1R$.

• BMB Reports
• /
• 제37권4호
• /
• pp.480-486
• /
• 2004

Brain ischemia brings about hypoxic insults. Hypoxia is one of the major pathological factors inducing neuronal injury and central nervous system infection. We studied the involvement of mitogen-activated protein (MAP) kinase in hypoxia-induced apoptosis using cobalt chloride in C6 glioma cells. In vitro cytotoxicity of cobalt chloride was tested by MTT assay. Its $IC_{50}$ value was $400\;{\mu}M$. The DNA fragment became evident after incubation of the cells with $300\;{\mu}M$ cobalt chloride for 24 h. We also evidenced nuclear cleavage with morphological changes of the cells undergoing apoptosis with electron microscopy. Next, we examined the signal pathway of cobalt chloride-induced apoptosis in C6 cells. The activation of extracellular signal-regulated protein kinase 1/2 (ERK 1/2) started to increase at 1 h and was activated further at 6 h after treatment of 400 M cobalt chloride. In addition, pretreatment of PD98059 inhibited cobalt chloride-induced apoptotic cell morphology in Electron Microscopy. These results suggest that cobalt chloride is able to induce the apoptotic activity in C6 glioma cells, and its apoptotic mechanism may be associated with signal transduction via MAP kinase (ERK 1/2).

• 한국전자파학회논문지
• /
• 제27권3호
• /
• pp.291-298
• /
• 2016

본 논문에서는 전자파적합성 국제 규격인 IEC 61000-4-4에서 규정하고 있는 전기적으로 빠른 과도현상/버스트(EFT/Burst) 신호가 전원선을 따라 시험대상품에 인가될 때, 인가신호에 기인하여 방사되는 불요전자파와 그 신호가 피시험품에 미치는 영향에 대해 연구하였다. 먼저 국제규격에 규정된 시험 배치방법에 따라 시험 샘플을 설치하고, 검증된 EFT/Burst 발생기로 1분 동안 +4 kV의 규정된 신호를 길이 1 m인 전원선을 통해 시험 샘플에 인가하였다. 그 결과, 전원선을 통해 인가되는 전도성 EFT/Burst 내성 신호 외 버스트 발생기 및 전원선에서 직접 공기 중으로 방출되는 방사성 신호(EFT/Burst 노이즈)가 검출되었고, 국제 공인시험 규격으로 이 신호를 측정한 결과, 규제 레벨보다 30 dB 이상 높았다. 또한, EFT/Burst 방사성 신호의 최대 방사 지점과 시험품의 이격 거리에 따른 전계강도를 측정한 결과, 방사내성 국제 규격치보다 최대 23 V/m 이상 강한 전계강도가 검출되었다. 이는 EFT/Burst 방사성 노이즈가 직접적으로 제품에 인가되어 오동작을 유발할 가능성이 대단히 높으므로 대책이 요구됨을 확인하였다.

• Journal of the Optical Society of Korea
• /
• 제9권1호
• /
• pp.13-15
• /
• 2005

It is shown that the SSB modulated signal has smaller chromatic-dispersion induced penalty than the DSB modulated signal only when the accumulated dispersion is large. When the accumulated dispersion is small, the SSB modulated signal has larger dispersion penalty than the DSB modulated signal. Therefore, if one builds a system with very low dispersion penalty, SSB modulation may end up with a larger dispersion penalty.

• 한국연소학회지
• /
• 제12권4호
• /
• pp.1-7
• /
• 2007

Carbon monoxide (CO) is not only an important intermediate species in chemical reaction mechanisms of hydrocarbon fuel combustion, but also a crucial pollutant species emitted from automotive engines. To better understand the physical processes impacting CO emissions, the development of laser-based measurement techniques that can visualize in-cylinder CO distributions is desirable. Among these techniques, Laser-Induced Fluorescence (LIF) is a sensitive and species-selective detection technique capable of good spatial resolution. However, some technical matters such as deep UV excitation, severe pressure dependency of the LIF signal, and potential interference from other species have been major challenges for CO LIF application. This study is focused on investigating the feasibility of CO two-photon LIF in a direct-injection diesel engine operating at typical pressure and temperature conditions with commercial grade diesel fuel. Spectroscopic analysis shows that the CO fluorescence signal can be separated from $C_2$ Swan band or broadband fluorescence from PAHs when the signal is collected near 483 nm. The signal-to-noise ratio of CO LIF deteriorate rapidly as pressure is increased, following $P^{-1.49}$ which matches the theoretical signal pressure dependency.