• 한국토양비료학회지
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• 제20권3호
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• pp.275-283
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• 1987

전보(前報)에 이어 토양(土壤)의 특성(特性)이 상이(相異)한 대두재배지(大豆栽培地)로 부터 토착대두근류균(土着大豆根瘤菌)을 분리(分離)하여 유리(遊離) 상태(狀態)에서 질소환원작용(窒素還元作用)과 균체단백질(菌體蛋白質)의 전기영동(電氣泳動) pattern 상의 차이(差異)를 1차원(次元) 및 2차원(次元) 전기영동상(電氣泳動像)으로 분석(分析)한 결과(結果)는 다음과 같다. 1. 토착대두류균(土着大豆瘤菌)의 생육형(生育型) 및 이화적(異化的) 질산환원작용(窒酸還元作用)을 조사(調査)한 결과(結果) 4개군(個群)으로 구분(區分)되었다. 즉(卽) 총(總) 87개(個) 검정균주(檢定菌株)중 fast-grower는 38.7%, slow-grower는 61.3%로서 fast grower 보다 많았다. 2. 이들 각(各) 생육형간(生育型間)에는 탈질형(脫窒型)과 비탈질형(非脫窒型)(질산호흡형(窒酸呼吸型))으로 구분(區分)되었으나, 질산환원효소결여형(窒酸還元酵素缺如型)은 나타나지 않았다. fast-grower와 slow-grower 간(間)에 탈질형(脫窒型)은 전자(前者) 31%, 후자(後者)에서 89%, 그리고 비탈질형(非脫窒型)은 전자(前者) 69%, 후자(後者)에서 11%로 나타났다. 3. 토착대두근류균(土着大豆根瘤菌)의 균체단백질(菌體蛋白質)의 특성(特性)은 1차원(次元) 및 2차원(次元) 전기영동상(電氣泳動像)에서 fast-grower와 slow-grower의 양생육형간(兩生育型間)에는 차이(差異)를 볼수 있었으나, 탈질형(脫窒型)과 비탈질형간(非脫窒型間)에는 현저(顯著)한 차이(差異)를 나타내지 않았다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권9호
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• pp.1223-1228
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• 2012

The population sizes of three Korean indigenous cattle populations have been drastically reduced over the past decades. In this study, we examined the extent to which reduction in populations influenced genetic diversity, population structure and demographic history using complete mitochondrial DNA (mtDNA) control region sequences. The complete mtDNA control region was sequenced in 56 individuals from Korean Black (KB), Jeju Black (JEB) and Korean Brindle (BRI) cattle populations. We included 27 mtDNA sequences of Korean Brown (BRO) from the GenBank database. Haplotype diversity estimate for the total population was high (0.870) while nucleotide diversity was low (0.004). The KB showed considerably low nucleotide (${\pi}$ = 0.001) and haplotype (h = 0.368) diversities. Analysis of molecular variance revealed a low level of genetic differentiation but this was highly significant (p<0.001) among the cattle populations. Of the total genetic diversity, 7.6% was attributable to among cattle populations diversity and the rest (92.4%) to differences within populations. The mismatch distribution analysis and neutrality tests revealed that KB population was in genetic equilibrium or decline. Indeed, unless an appropriate breeding management practice is developed, inbreeding and genetic drift will further impoverish genetic diversity of these cattle populations. Rational breed development and conservation strategy is needed to safeguard these cattle population.

• Geomechanics and Engineering
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• 제17권5호
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• pp.485-496
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• 2019

Sealing of leakage in waterfront or water-retaining structures is one of the major issues in geotechnical engineering practices. With demands for biological methods as sustainable ground improvement techniques, bioclogging, defined as the reduction in hydraulic conductivity of soils caused by microbial activities, has been considered as an alternative to the chemical grout techniques for its economic advantages and eco-friendliness of microbial by-products. This study investigated the feasibility of bioaugmentation and biostimulation methods to induce fermentation-based bioclogging effect in coarse sands. In the bioaugmentation experiments, effects of various parameters and conditions, including grain size, pH, and biogenic gas generation, on hydraulic conductivity reduction were examined through a series of column experiments while Leuconostoc mesenteroides, which produce an insoluble biopolymer called dextran, was used as the model bacteria. The column test results demonstrate that the accumulation of bacterial biopolymer can readily reduce the hydraulic conductivity by three-to-four orders of magnitudes or by 99.9-99.99% in well-controlled environments. In the biostimulation experiments, two inoculums of indigenous soil bacteria sampled from waterfront embankments were prepared and their bioclogging efficiency was examined. With one inoculum containing species capable of fermentation and biopolymer production, the hydraulic conductivity reduction by two orders of magnitude was achieved, however, no clogging was found with the other inoculum. This implies that presence of indigenous species capable of biopolymer production and their population, if any, play a key role in causing bioclogging, because of competition with other indigenous bacteria. The presented results provide fundamental insights into the bacterial biopolymer formation mechanism, its effect on soil permeability, and potential of engineering bacterial clogging in subsurface.

• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1387-1402
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• 2005

Indian subcontinent is a rich source of diverse ovine germplasm, and only very few countries have such a large number of breeds with wide genetic diversity. This vast ovine biodiversity in India is being eroded rapidly and more than 50% of sheep breeds are currently under threat. It is noteworthy that the characterization of Indian sheep breeds was last done in the first half of the century since then no recent estimates are available and surveys in majority of the regions/breeds are far from complete. Starting in 1985 National Bureau of Animal Genetic Resources (NBAGR), Karnal, India, therefore, initiated activities aimed at determining the status and compiling information on indigenous farm animals including sheep. This report represents attempts made to date on the basis of field/literature surveys and additional activities on molecular characterization to ascertain their status including distribution, population changes, breed diversities and risk status. The need, mode and mechanisms of conservation are also described. Involvement of several agencies for evaluation, improvement, conservation programmes and recommendations made for effective characterization and conservation of sheep biodiversity are highlighted. This publication would promote action particularly at national level to improve the information base on domestic Indian breeds of sheep and provide input into national domestic sheep diversity conservation policy decisions.

• Journal of Species Research
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• 제9권4호
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• pp.427-442
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• 2020

We collected indigenous Korean ciliate species from diverse aquatic and terrestrial habitats in 2018 and 2019. The morphology of these ciliates was revealed based on the observations of living cells, and protargol-impregnated and/or silver carbonate-impregnated specimens. During this study, we found 16 previously unrecorded Korean ciliate species, which are as follows: 1) class Heterotrichea - Stentor introversus; 2) class Spirotrichea - Aspidisca orthopogon, Amphisiella sinica, Epiclintes auricularis rarisetus, Apokeronopsis wrighti, Pseudokeronopsis carnea, Trachelostyla pediculiformis, Strombidium apolatum, and Varistrombidium kielum; 3) class Phyllopharyngea - Chlamydodon obliquus, Dysteria aculeata, and Hartmannula angustipilosa; 4) class Litostomatea - Paraspathidium apofuscum; and 5) class Oligohymenophorea - Frontonia angusta solea, Metanophrys sinensis, and Uronemita binucleata. Here, we provide a diagnosis for each species with a brief remark. Among them, the infraciliature of the poorly known species, Stentor introversus and Dysteria aculeata, is described for the first time. Further, we revise the Korean population of Pseudokeronopsis pararubra, which was previously misidentified as Pseudokeronopsis carnea.

• 환경생물
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• 제32권1호
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• pp.88-94
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• 2014

종의 장거리 산포는 집단의 확산과 집단간 개체의 흐름에 중요한 역할을 한다. 본 연구는 독도에서 발견된 식물을 형태학적 특징과 핵 및 엽록체 DNA의 분자마커를 이용하여 종을 식별한 결과, 참빗살나무 (노박덩굴과)로 확인되었다. 얕은 토양층과 험준한 지형 등 입지적으로 열악한 대양섬인 독도에서 목본의 분포는 의미있는 결과이며, 향후 외부유입종의 지속적인 모니터링이 요구된다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권2호
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• pp.277-281
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• 2003

Amplified fragment length polymorphism (AFLPs) markers were used to investigate the genetic variation in six autochthonous goat populations distributed in the middle and lower Yangtze River valley. The goat populations were Chengdu Grey Goat (CGG), Chuandong White Goat (CWG), Banjiao Goat (BG), Matou Goat (MG), Hui Goat (HG) and Yangtze River Delta White Goat (YRDWG). A total of 180 individuals (30 per population) were analysed using ten selected AFLP primer combinations that produced 78 clear polymorphism loci. The variability at AFLP loci was largely maintained within populations, as indicated by the average genetic similarity, and they were ranged from 0.745 to 0.758 within populations and 0.951 to 0.970 between populations. No breed specific markers were identified. Cluster analysis based on Nei' genetic distance between populations indicated that Chengdu Grey Goat is the most distant population, while CWG and YROWG were the closest populations, followed by BG, HG and MG. Genetic diversity of the goat populations didn' confirm what was expected on the basis of their geographical location, which may reflect undocumented migrations and gene flows and identify an original genetic resource.

• Journal of Applied Biological Chemistry
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• 제48권4호
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• pp.161-166
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• 2005

Among 24 isolates of fluorescent Pseudomonads, 5 isolates named as LPT1, LPT2, LPT3, LPT4 and LPT5 were screened in vitro for their nematicidal activity against cyst forming nematode, Heterodera cajani causing patchiness, poor and stunting growth besides discoloration in Sesamum indicum. Second stage juveniles of H. cajani hatched from egg masses were collected from roots of host plant and subjected to fresh and heat-treated culture filtrate of isolates for 24 h. Mortality of H. cajani was recorded on the basis of parameters used for test organism bioassay. Among these isolates, Pseudomonas aeruginosa LPT5 caused maximum mortality towards second stage juvenile of H. cajani in vitro. Five isolates were used as seed coating for the management of cyst forming nematode H. cajani on sesame in green house condition. The strains LPT5 was better than the other strains in reducing the population of H. cajani both in vitro and in vivo. The reduction in cyst and juveniles population was found to be 49 and 60%, respectively when seeds were coated with strain LPT5. Among other strains, LPT4 was also found to inhibit the cyst and juveniles population 12 and 36% respectively. Increases in early vegetative plant growth parameters recorded in both in vitro and in vivo further revealed the significance of indigenous bacteria in comparison to introduced strain.

• Animal Bioscience
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• 제35권7호
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• pp.949-954
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• 2022

Objective: The present study is aimed at phenotypic characterization and mitochondrial d-loop analysis of indigenous "Diara" buffalo population, which are mostly confined to the villages on the South and North Gangetic marshy plains in the Bihar state of India. These buffaloes are well adapted and are best suited for ploughing and puddling the wet fields meant for paddy cultivation. Methods: Biometric data on 172 buffaloes were collected using a standard flexible tape measure. Animals are medium in size; the typical morphometric features are long head with a broad forehead and moderately long and erect ears. Genomic DNA was isolated from unrelated animals. The mtDNA d-loop 358-bp sequence data was generated and compared with 338 sequences belonging to riverine and swamp buffaloes. Results: Based on the mitochondrial d-loop analysis the Diara buffaloes were grouped along with the haplotypes reported for riverine buffalo. Sequence analysis revealed the presence of 7 mitochondrial D loop haplotypes with haplotype diversity of 0.9643. Five of the haplotypes were shared with established swamp breeds and with Buffalo population of Orissa in India. Conclusion: Morphometric analyses clearly shows distinguishing features like long and broad forehead which may be useful in identification. The germplasm of Diara buffalo is much adapted to the marshy banks of river Ganga and its tributaries. It constitutes a valuable genetic resource which needs to be conserved on priority basis.

• Asian-Australasian Journal of Animal Sciences
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• 제17권5호
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• pp.598-602
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• 2004

The Tongcheng pig breed is a famous Chinese indigenous breed. The Ministry of Agriculture of China has filed it as 1 of 19 national key conservation breeds selected from more than 100 Chinese indigenous pig breeds in 2000. In order to improve the reproductive performance, it has been intensively selected to increase the litter size for about 10 years. The population randomly sampled from conservation nucleus of eight families in the Tongcheng pigs was genotyped for identification of their estrogen receptor locus polymorphisms with the PCR-RFLPs method. Only AB heterozygotes and BB homozygotes were detected, and $X^2$ test demonstrated that the locus was in disequilibrium at a significant level (p<0.05). In the present paper, the litter sizes in different parities were regarded as different traits. Holistic status of other unspecific and unidentified genes was estimated by using the statistical methods. Coefficients of kurtosis and skewness showed that the litter size still presented segregating characteristic in the 2nd, 5th, 7th, 8th and 9th parities. Analysis of homogeneity of variance between families confirmed the results for the 5th, 7th and 8th parities. The heritability of litter size for the 1st to 10th parities was estimated with paternal half-sib model and individual estimated breeding values (EBVs) were evaluated by a single trait animal model as well. We found that the averages of EBVs for litter size in each parity did not differ significantly between genotypes, despite the significant difference for original phenotype records in the 3rd, 4th and 5th parities (p<0.05 or p<0.01). The results may be explained by the deduction that the polymorphisms of ESR locus are no longer the important genetic base of litter size variation when the frequency of allele B accumulated in the experience of selection procedure, and further conferring that there exist special genes associated with litter size in the recent Tongcheng pigs population can be made.