• 한국식품위생안전성학회지
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• 제11권1호
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• pp.57-70
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• 1996

This paper intends to investigate commercial fish and shellfish 25 species (fish 8 species, shellfish 7 species, crustacean 3 species, moluse 4 species and echinodermata) for the distribution of sanitary indicator organisms (total viable counts, coliforms, staphylococci, vibrios, and enterococci) and distributional change of indicator organisms accordion to storage temperature and period. The logarithmic mean of total viable counts for total commercial fish and shellfish 25 species was 5.41$\pm$.026 CFU/g, and in accordance with fish and shellfishes, crustacean 6.76$\pm$0.67 CFU/g, shellfish 5.67$\pm$0.56 CFU/g, echinodermata 5.47$\pm$1.50 CFU/g, fish 5.02$\pm$0.38 CFU/g, and mollusc 5.03$\pm$0.65 CFU/g. The logarithmic mean of enterococci was 2.36$\pm$0.37 CFU/g, and in accordane with fish and shellfish, crustacean 3.44$\pm$2.12 CFU/g, shellfish 3.87$\pm$0.45 CFU/g, echinodermata 3.38$\pm$0.0 CFU/g, fish 2.16$\pm$0.41 CFU/g and mollusc 0.01$\pm$0.0 CFU/g. The logarithmic mean of vibrios was 1.60$\pm$1.59 CFU/g, and in accordance with fish and shellfish, crustacean 4.23$\pm$1.11 CFU/g, shellfish 3.58$\pm$0.90 CFU/g, echinodermata 1.64$\pm$0.34 CFU/g, fish 1.79$\pm$0.67 CFU/g and mollusc 1.07$\pm$0.61 CFU/g. The logarithmic mean of staphylococci was 1.60$\pm$1.59 CFU/g, and in accordance with fish and shellfish, shellfish 0.01$\pm$0.00 CFU/g, echinodermata 3.51$\pm$0.60 CFU/g, fish 1.68$\pm$0.64 CFU/g, crustacean 0.34$\pm$0.33 and mollusc 3.90$\pm$0.11 CFU/g. The logarithmic mean of coliformas was 2.52$\pm$0.32 CFU/g, and in accordance with fish and shellfish, echinodermata 3.58$\pm$1.89 CFU/g was highest, shellfish 3.25$\pm$0.30 CFU/g, crustacean 3.23$\pm$0.49 CFU/g, fish 2.18$\pm$0.63 CFU/g, peeled shellfish 1.80$\pm$0.51 CFU/g and mollusc 1.55$\pm$0.95 CFU/g. As the results of research of the change of the contaminate indicator microflora in working with storage period at 1$0^{\circ}C$, 2$0^{\circ}C$ and 3$0^{\circ}C$, total viable counts was increased without storage temperature and enterococci were decreased slowly at 1$0^{\circ}C$, but increased at 20 and 3$0^{\circ}C$. Vibrios were decreased slowly at 1$0^{\circ}C$, decreased at 2$0^{\circ}C$ and 3$0^{\circ}C$in 2 days after increased rapidly. Staphylococci were increased promptly without storage temperature in 2 days, then the total viable counts were maintained. Coliforms were increased at 1$0^{\circ}C$ by 7 days, then decreased or maintained after 14 days, changed at 2$0^{\circ}C$ in accordance with fish species in 2 days, then returned to the initial total viable count, and decreased rapidly at 3$0^{\circ}C$ on 2 days. By the way, there were no difference among the species.

• Asian-Australasian Journal of Animal Sciences
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• 제6권3호
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• pp.441-445
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• 1993

Ten bacteriocin-producing Enterococcus faecium strains with urease activity ($1.10-6.2nkat.mL^{-1}$) were isolated from the rumen of 2-8 weeks old calves. All strains were resistant aginst disodium arsenate at a minimal inhibition concentration - MIC $5g.L^{-1}$ and mercury chloride ($MIC=10-20mg.L^{-1}$). Eight strains were resistant against silver nitrate ($MIC=40-50mg.L^{-1}$) and three against antibiotics used. The resistance against six antibiotics was found in A23 strain. Values of adherence index ranged from 5.02 to 20.4 enterococci adhered per one epithelial cell of rumen wall. All isolates produced bacteriocins which inhibited the growth at least of one of five indicator organisms. The EF1 strain with a good affinity to the epithelial cell ($15.2{\pm}1.2$) produced bacteriocin substance with antimicrobial activity against grampositive and gramnegative indicator bacteria.

• Asian-Australasian Journal of Animal Sciences
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• 제11권3호
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• pp.209-216
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• 1998

The paper presented here is aimed at increasing knowledge on purine metabolism in ruminants and hence the quantification of microbial cells entering the small intestine from urinaη excretion of purine derivatives. Nucleic acid metabolisms of micro-organisms in the rumen, digestion and absorption of nucleic acids entering the intestines, metabolisms of absorbed and endogenous purines involving de novo synthesis of nucleic acids in the ruminants host, and the relationship between absorbed and excreted purines are reviewed. Principal concerns about an amount of purine derivatives excreted in urine in relation to a change in purine-N: total-N ratios in rumen microbes that leave the rumen are discussed. The use of urinary excretion of purine derivatives as an indicator of the amount of microbial biomass leaving the rumen has to be done with some caution since it may be impossible to get a representative sample of microbes entering the intestine and thus yield estimates are relative rather than absolute.

• 한국해양학회지
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• 제11권2호
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• pp.71-76
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• 1976

This work concerns with the biotic study of the polychaeta population in the intertidal flat, Inchon, Korea. Magelona japonica was found to be the most abundant species, comprising 54% of total organisms examined. The presence of large numbers of Magelona japonica and Sternaspis scutata, high value of species diversity indices (H(s)), and the absence of the particular pollution indicator species, except a few Prinospio pinnata, suggest that the study area appears not to be polluted during the study period.

• 농업과학연구
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• 제39권4호
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• pp.545-553
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• 2012

In Asia, including Japan and Korea, rice paddies occupy the largest cultivated area in agricultural land. Rice paddies provide the habitats for many organisms including endemic species, sustaining high biodiversity. Insect natural enemies inhabiting rice paddies have an important function for rice production as agents of 'ecosystem services' because they play a major role in suppressing rice pests. The diversity and abundance of natural enemies can be a good index reflecting the 'healthiness' of agro-ecosystem services in rice paddies. The present study investigates whether insect natural enemies could be good biological indicators for general arthropod biodiversity and agricultural practice. First, the concept of ideal bio-indicators was summarized. The strategy to explore and select such bio-indicators was then proposed. Lastly, field survey was made to evaluate the abundance and biodiversity of natural enemies in Japanese rice paddies where chemical inputs, i.e., insecticide use, were different. The results showed that reduction of chemical inputs led to an increase in species richness or diversity of natural enemies including parasitoids and predators. Then, the data were analyzed to examine suitable indicator species to assess environmental soundness of agricultural practice and biodiversity in rice paddies. The density of several species of natural enemies did respond both to pesticide use and to general arthropod biodiversity. The analyses thus have indicated that natural enemies can be suitable as bio-indicators. Usefulness of indicator species in rice paddies is discussed in the context of ecologically sound agriculture.

• 한국수산과학회지
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• 제56권5호
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• pp.721-727
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• 2023

This study was conducted to evaluate the level of microbial contamination in smoked salmon products sold in hypermarkets in major metropolitan cities in Korea. Listeria monocytogenes is the primary cause of smoked and raw salmon product recalls. Here, we used L. monocytogenes as a bacterial hygiene indicator and investigated the microbial contamination level of frozen/refrigerated smoked salmon products collected from hyper markets. Contamination levels were analyzed by seasons, manufacturers, and consumption regions. For hygiene indicator bacteria, total bacteria count, coliforms, and Escherichia coli were examined according to the food code established by the Ministry of Food and Drug Safety, and polymerase chain reaction (PCR) was performed to detect Listeria sp. The highest contamination level was observed in spring in Busan among five consumption regions. Listeria was detected at a level of 2.1% among all samples collected. And especially L. monocytogenes was detected in two cases from the samples collected from Daegu and Daejeon. Sanitary standards and specifications should be established according to the contamination level of smoked salmon products investigated in this study, and continuous monitoring is necessary.

• 한국환경보건학회지
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• 제41권2호
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• pp.82-89
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• 2015

Objectives: This study was performed to investigate the effect of cooking time on the internal temperature and color of cutlets and the reduction of indicator organisms in cutlets by cooking. Methods: Three kinds of commercially packed frozen cutlets (pork, chicken and fish cutlets), were purchased from local markets. The cutlets were cooked in a frying pan at $180^{\circ}C$ for four minutes. Internal temperature was measured with a food thermometer. Color was measured using a Hunter spectrocolorimeter. Aerobic colony counts, coliforms, and Escherichia coli were determined according to the Food Code of Korea. Results: The internal cooked temperature of every cutlet reached over $74^{\circ}C$, the temperature considered safe, after three minutes, while external temperature reached this level in two minutes (p < 0.001). The instrumental color value as lightness (L) in the cooked cutlets significantly changed (p < 0.001) after one minute. The level of aerobic colony counts of fresh cutlets was under the specification and was reduced to one tenth its level in the cooked cutlets. Coliforms and E. coli were not detected in all samples. The internal temperature of the cutlets was significantly affected by cooking time and weight (p < 0.001). The interaction effect of time and weight was also significant (p < 0.001), and time was the more influential factor. Conclusion: The results of this study indicate that the sampled cutlets should be cooked for a minimum of three minutes or more in order to ensure food safety. The results also indicate that if consumers cease cooking based on external temperature or color, there will be a risk of inadequate cooking.

• 생태와환경
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• 제52권1호
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• pp.1-8
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• 2019

River water quality and organisms have a very close relationship with the human living environment and health, so it is very important to ensure and maintain the ecological integrity of the aquatic ecosystem. In that sense, benthic diatoms have relatively little mobility, can explain the effects of long-term exposed pollution sources, and are very suitable indicator organisms for river ecosystem evaluation. Diatom ecologists have been developed various diatom indices to assess water quality and stream ecosystem over the world. However, they so far have insufficient identification of taxa, are strongly regional, and are difficult to apply as they are domestically. Unfortunately, there has not been developed an independent diatom index suitable for the Korean stream. Therefore, management of water quality and aquatic ecosystem suitable for domestic rivers can be made, and development or improvement of comprehensive multivariate diatom index for the integrated assessment of water quality and aquatic ecosystem is urgently needed.

• 한국물환경학회지
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• 제35권2호
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• pp.152-164
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• 2019

In this study, a Benthic Macroinvertebrates Family Index (BMFI) was developed using 100 indicator groups (99 families including Chironomidae with 2 phena). Families were assigned a score between 1 and 10 depending on their sensitivity to organic pollution. The BMFI was composed of the sensitivity and relative abundance of the indicator taxa. Sensitivity values of each group were generally similar to Biological Monitoring Working Party (BMWP) scores or Walley, Hawkes, Paisley, Trigg (WHPT) scores of UK, Japanese BMWP scores, and the FBI tolerance values of North America. However, sensitivity values of some taxa were significantly different from those of foreign countries, which seemed to have resulted from discrepancy in species composition, difference of taxonomic classification system, or methodological difference for estimation of sensitivity. As an annual average level, BMFI showed significant correlation with concentration of 5-day biochemical oxygen demand (BOD5) (correlation coefficient r = -0.80, n = 569 sites), total suspended solids (r = -0.68), and total phosphorus (r = -0.79). In addition, BMFI revealed strong correlation with Shannon-Weaver's species diversity (r = 0.85), Margalef's species richness (r = 0.85) and McNaughton's dominance (r = -0.84). Correlation between BMFI and water quality parameters or community indices such as species diversity did not show significant difference compared to that of species-level indices such as BMI (Benthic Macroinvertebrates Index). This means that BMFI is a more useful indicator in terms of easy identification of organisms. BMFI was used to assess the environmental status of 3,017 sites of Stream Ecosystem Survey conducted by the Korean Ministry of Environment between 2016 and 2018. As a result, about half of all sites appeared to be in good condition, and a quarter in poor condition.

• 한국해양학회지
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• 제10권1호
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• pp.17-24
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• 1975

In order to clarify the accumulation of radioactive materials in marine organisms of Korea, the present investigation is carried out with 54 samples of edible seaweeds collected from eight sampling sites along the coast of Korea during September, 1973 and April, 1974. In this paper, ash contents, gross alpha activities and gross beta activities are detected. The ash content is 7.53- 15.95% in the species investigated. Among the algal phyla it is about 13.13% in green algae, 12.77% in brown algae, and 10.77% in red algae on an average. On the other hand, gross alpha activities fluctuate from 180.0 pCi/Kg to 1082.6 pCi /Kg-fresh material experimented, and are 530.72 pCi/Kg on an average. They increase from green to red and brown algae, in turn. The activities in a single species collected at the same season increse from eastern to western and southern coasts of Korea, in turn. Gross beta activities, however, fluctuate from 2.40 nCi/Kg to 22.14 nCi/Kg-fresh material experimented, and 9.03 nCi/Kg on an average. They increase also from green to red nd brown algae, in turn. The gross beta activities are specially higher in Sargassum thunbergii, 22.14 nCi/Kg It is expected that this plant could be an indicator to detect the activities in the marine algae along the coast of Korea.