• International Journal of Industrial Entomology and Biomaterials
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• 제32권2호
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• pp.60-68
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• 2016

Pheromone orientation in moths is an exemplar of olfactory sensitivity. To avoid cross mating, the responses of males to pheromone blends must be high specificity and temporal resolution. We tested the effects of olfactory self- and cross-adaptation of pheromone compounds and mixtures in Spodoptera litura moths by electroantennogram (EAG) recordings. The challenge of S. litura antennae to a pulse train of its own pheromone blends of Z9,E11-14:OAc and Z9,E12-14:OAc with 200 ms on/off and 1 s on/off indicated that the repetitive stimulation by 200 ms on/off with high dosages resulted in greater adaptation than that by 1 s on/off with low dosages and the adaptation index of Z9,E11-14:OAc in all treatments is significantly larger than that of Z9,E12-14:OAc, suggesting that high dosages with more frequent stimulation prefer to induce sensory adaptations and a different odor coding exist between the two components in the antennal periphery in this moth. The cross-adaptation EAG test among the two pheromone compounds and Z7-12:OAc and Z9-14:OH from congeneric species of S. litura showed that each of these compounds adapted the antenna more to that specific compound. The significantly higher adaptation to Z7-12:OAc and Z9-14:OH than to the pheromone components of S. litura induced by themselves suggested that both of them are coded by specific odor receptor neurons which are different from those tuned to the pheromone components of S. litura . Thus, we proposed that Z7-12:OAc and Z9-14:OH may play an important role in avoidance of heterospecific mating between S. litura and its sympatric moth species.

• Nutrition Research and Practice
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• 제7권4호
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• pp.294-301
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• 2013

In this study, we examined the hepatic anti-steatosis activity of carnosic acid (CA), a phenolic compound of rosemary (Rosmarinus officinalis) leaves, as well as its possible mechanism of action, in a high-fat diet (HFD)-fed mice model. Mice were fed a HFD, or a HFD supplemented with 0.01% (w/w) CA or 0.02% (w/w) CA, for a period of 12 weeks, after which changes in body weight, blood lipid profiles, and fatty acid mechanism markers were evaluated. The 0.02% (w/w) CA diet resulted in a marked decline in steatosis grade, as well as in homeostasis model assessment of insulin resistance (HOMA-IR) index values, intraperitoneal glucose tolerance test (IGTT) results, body weight gain, liver weight, and blood lipid levels (P < 0.05). The expression level of hepatic lipogenic genes, such as sterol regulating element binding protein-1c (SREBP-1c), liver-fatty acid binding protein (L-FABP), stearoyl-CoA desaturase 1 (SCD1), and fatty acid synthase (FAS), was significantly lower in mice fed 0.01% (w/w) CA and 0.02% (w/w) CA diets than that in the HFD group; on the other hand, the expression level of ${\beta}$-oxidation-related genes, such as peroxisome proliferator-activated receptor ${\alpha}$ (PPAR-${\alpha}$), carnitine palmitoyltransferase 1 (CPT-1), and acyl-CoA oxidase (ACO), was higher in mice fed a 0.02% (w/w) CA diet, than that in the HFD group (P < 0.05). In addition, the hepatic content of palmitic acid (C16:0), palmitoleic acid (C16:1), and oleic acid (C18:1) was significantly lower in mice fed the 0.02% (w/w) CA diet than that in the HFD group (P < 0.05). These results suggest that orally administered CA suppressed HFD-induced hepatic steatosis and fatty liver-related metabolic disorders through decrease of de novo lipogenesis and fatty acid elongation and increase of fatty acid ${\beta}$-oxidation in mice.

• Journal of Korean Medical Science
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• 제33권51호
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• pp.324.1-324.6
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• 2018

Oxysterol $7{\alpha}$-hydroxylase deficiency is a very rare liver disease categorized as inborn errors of bile acid synthesis, caused by CYP7B1 mutations. As it may cause rapid progression to end-stage liver disease even in early infancy, a high index of suspicion is required to prevent fatal outcomes. We describe the case of a 3-month-old boy with progressive cholestatic hepatitis and severe hepatic fibrosis. After excluding other etiologies for his early liver failure, we found that he had profuse urinary excretion of $3{\beta}$-monohydroxy-${\Delta}^5$-bile acid derivatives by gas chromatography/mass spectrometry analysis with dried urine spots on filter paper. He was confirmed to have a compound heterozygous mutation (p.Arg388Ter and p.Tyr469IlefsX5) of the CYP7B1 gene. After undergoing liver transplantation (LT) from his mother at 4 months of age, his deteriorated liver function completely normalized, and he had normal growth and development until the current follow-up at 33 months of age. We report the first Korean case of oxysterol $7{\alpha}$-hydroxylase deficiency in the youngest infant reported to undergo successful living donor LT to date.

• International Journal of Industrial Entomology and Biomaterials
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• 제33권2호
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• pp.149-149
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• 2016

Pheromone orientation in moths is an exemplar of olfactory sensitivity. To avoid cross mating, the responses of males to pheromone blends must be high specificity and temporal resolution. We tested the effects of olfactory self-and cross-adaptation of pheromone compounds and mixtures in Spodoptera litura moths by electroantennogram (EAG) recordings. The challenge of S. litura antennae to a pulse train of its own pheromone blends of Z9,E11-14:OAc and Z9,E12-14:OAc with 200 ms on/off and 1 s on/off indicated that the repetitive stimulation by 200 ms on/off with high dosages resulted in greater adaptation than that by 1 s on/off with low dosages and the adaptation index of Z9,E11-14:OAc in all treatments is significantly larger than that of Z9,E12-14:OAc, suggesting that high dosages with more frequent stimulation prefer to induce sensory adaptations and a different odor coding exist between the two components in the antennal periphery in this moth. The cross-adaptation EAG test among the two pheromone compounds and Z7-12:OAc and Z9-14:OH from congeneric species of S. litura showed that each of these compounds adapted the antenna more to that specific compound. The significantly higher adaptation to Z7-12:OAc and Z9-14:OH than to the pheromone components of S. litura induced by themselves suggested that both of them are coded by specific odor receptor neurons which are different from those tuned to the pheromone components of S. litura. Thus, we proposed that Z7-12:OAc and Z9-14:OH may play an important role in avoidance of heterospecific mating between S. litura and its sympatric moth species.

• 융합정보논문지
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• 제9권11호
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• pp.125-133
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• 2019

본 연구에서는 홍수시나리오에 의해 발생한 제방붕괴에서 불확실성을 포함한 확률홍수위험지도를 산정하였다. 불확실성을 포함한 극치수문시나리오와 그에 따른 홍수위가 산정된 선행연구자료를 활용하였고 이에 따라 제방붕괴 예측지점을 산정하였다. 단순한 조건에 따른 일률적인 파제폭을 제시하는 경험식과 지반공학적 복합요소들에 대한 불확실성을 고려한 물리적 기반의 수치모의 방식을 결합하여 파제폭을 산정하였다. 이에 따라 확률론적 파제유입량을 결정하였고, 신뢰도를 기반으로 100회 모의수행을 통한 2차원 제내지 침수해석을 실시하여 확률침수심도를 작성하였다. 이를 통해 홍수위험지도 작성기법을 기반으로 확률침수심도와 결합하여 확률홍수위험지도를 작성하였다. 본 연구결과는 제내지의 비상대처계획(EAP)의 정량적 근거자료로 보다 경제적, 안정적인 설계지표 제시하는데 효과적일 것으로 기대된다.

• 대한본초학회지
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• 제33권3호
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• pp.1-9
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• 2018

Objectives : In Korean medicine, a prescription of Sinsun-yukza-hwan (Shenxian-liuzi-wan, SSY) has been used in clinic for treatment of alopecia via oral. This study was performed to determine transdermal effects of the ethanol extract from SSY on hair growth and -related gene expressions in mice. Methods : We analyzed index compound, 5-hydroxy-methyl-2-furaldehyde (HMF), in SSY extract by ultra performance liquid chromatography (UPLC). 6 weeks old C57BL/6 mice with removed hair were used as an alopecia animal model. Mice were divided into 3 experimental groups including normal (3 water: 1 ethanol: 2 polyethylene glycol mixture as a vehicle), SSY extract and 5% minoxidil (as a positive control), treated groups. SSY was applied topically on the hair-shaved skin of C57BL/6 mice every day for 15 days. The color, thickness and density of hair were monitored every $5^{th}$ day by naked eye, photograph and phototrichogram using folliscope. Also hair growth-associated gene expressions were measured by immunoblotting assay. Results : Hair density of minoxidil or SSY-treated group was significantly increased compared to that of vehicle application on the $15^{th}$ day, respectively. And hair thickness of minoxidil and SSY groups was increased compared to that of vehicle treated group on the $15^{th}$ day, respectively. Induction of insulin-like-growth factor 1(IGF-1) and vascular endothelial growth factor (VEGF) were also significantly accelerated by SSY extract compared to those of vehicle-applied group. Conclusions : These results provide scientific evidence to support the potent multi-application of SSY as a cosmeceutical material for promoting hair growth.

• Journal of Genetic Medicine
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• 제19권1호
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• pp.32-37
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• 2022

Mucopolysaccharidosis type VI (MPS VI) is an autosomal recessive lysosomal disorder caused by the deficiency of arylsulfatase B due to mutations in the ARSB gene. Here, we report the case of a Korean female with a novel variant of MPS VI. A Korean female aged 5 years and 8 months, who is the only child of a healthy non-consanguineous Korean couple, presented at our hospital for severe short stature. She had a medical history of umbilical hernia and recurrent otitis media. Her symptoms included snoring and mouth breathing. Subtle dysmorphic features, including mild coarse face, joint contracture, hepatomegaly, and limited range of joint motion, were identified. Radiography revealed deformities, suggesting skeletal dysplasia. Growth hormone (GH) provocation tests revealed complete GH deficiency. Targeted exome sequencing revealed compound heterozygous mutations in the ARSB genes c.512G>A (p.Gly171Asp; a pathogenic variant inherited from her father) and c.1157C>T (p.Ser386Phe; a novel variant inherited from her mother in familial genetic testing). Quantitative tests revealed increased urine glycosaminoglycan (GAG) levels and decreased enzyme activity of arylsulfatase B. While on enzyme replacement therapy and GH therapy, her height increased drastically; her coarse face, joint contracture, snoring, and obstructive sleep apnea improved; urine GAG decreased; and left ventricular mass index was remarkably decreased. We report a novel variant-c.1157C>T (p.Ser386Phe)-of the ARSB gene in a patient with MPS VI; these findings will expand our knowledge of its clinical spectrum and molecular mechanisms.

• Geomechanics and Engineering
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• 제33권1호
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• pp.29-40
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• 2023

Xanthan gum and starch compound biopolymer (XS), an environmentally friendly soil-binding material produced from natural resources, has been suggested as a slope protection material to enhance soil strength and erosion resistance. Insufficient wet strength and the consequent durability concerns remain, despite XS biopolymer-soil treatment showing high strength and erosion resistance in the dried state, even with a small dosage of soil mass. These concerns need to be solved to improve the field applicability and post-stability of this treatment. This study explored the utilization of an alkaline-based cation crosslinking method using calcium hydroxide and sodium hydroxide to induce non-thermal gelation, resulting in the enhancement of the wet strength and durability of biopolymer-treated soil. Laboratory experiments were conducted to assess the unconfined compressive strength and cyclic wetting-drying durability performance of the treated soil using a selected recipe based on a preliminary gel formation test. The results demonstrated that the uniformity of the gel structure and gelling time varied depending on the ratio of crosslinkers to biopolymer; consequently, the strength of the soil was affected. Subsequently, site soil treated with the recipe, which showed the best performance in indoor assessment, was implemented on the field slope at the bridge abutment via compaction and pressurized spraying methods to assess feasibility in field implementation. Moreover, the variation in surface soil hardness was monitored periodically for one year. Both slopes implemented by the two construction methods showed sufficient stability against detachment and scouring, with a higher soil hardness index than the natural slope for a year.

• 한국환경보건학회지
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• 제49권2호
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• pp.78-88
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• 2023

Background: Exposure to volatile organic compounds (VOCs) can have acute and chronic health effects on human beings in general and in working environments. In particular, VOCs are often emitted in large quantities in industrial settings. In such circumstances, there is a need to improve the indoor air quality at workplaces. Objectives: The purposes of this study were to verify the effectiveness of air cleaning devices in workplaces and provide alternative solutions for improving working environments. Methods: Personal exposure and area level of VOCs for workers were evaluated in a car-part adhesive process before and after installing an air cleaning device with a TiO₂-coated filter. Passive samplers and direct reading instruments were used to collect and analyze the VOCs, and the removal efficiency and improvement of air quality were evaluated. We also calculated the exposure index (EI) to assess the risk level in the workplace. Results: The removal efficiency for VOCs through the installation of the air cleaning device was approximately 26.9~69.0% as determined by the concentration levels before and after installation. The measured substances did not exceed the exposure limits for the work environment and the EI was less than 1. However, carcinogenic substances such as benzene, formaldehyde, carbon tetrachloride, and trichloroethylene were detected. Conclusions: The application of an air cleaning device can be a solution for controlling the indoor air quality in a workplace, particularly in cases where ventilation systems cannot be installed due to process limitations.

• Biomolecules & Therapeutics
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• 제31권1호
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• pp.27-39
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• 2023

Extensive research supported the therapeutic potential of curcumin, a naturally occurring compound, as a promising cytokine-suppressive anti-inflammatory drug. This study aimed to investigate the synergistic anti-inflammatory and anti-cytokine activities by combining 6-shogaol and 10-shogaol to curcumin, and associated mechanisms in modulating lipopolysaccharides and interferon-γ-induced proinflammatory signaling pathways. Our results showed that the combination of 6-shogaol-10-shogaolcurcumin synergistically reduced the production of nitric oxide, inducible nitric oxide synthase, tumor necrosis factor and interlukin-6 in lipopolysaccharides and interferon-γ-induced RAW 264.7 and THP-1 cells assessed by the combination index model. 6-shogaol-10-shogaol-curcumin also showed greater inhibition of cytokine profiling compared to that of 6-shogaol-10-shogaol or curcumin alone. The synergistic anti-inflammatory activity was associated with supressed NFκB translocation and downregulated TLR4-TRAF6-MAPK signaling pathway. In addition, SC also inhibited microRNA-155 expression which may be relevant to the inhibited NFκB translocation. Although 6-shogaol-10-shogaol-curcumin synergistically increased Nrf2 activity, the anti-inflammatory mechanism appeared to be independent from the induction of Nrf2. 6-shogaol-10-shogaol-curcumin provides a more potent therapeutic agent than curcumin alone in synergistically inhibiting lipopolysaccharides and interferon-γ induced proinflammatory mediators and cytokine array in macrophages. The action was mediated by the downregulation of TLR4/TRAF6/MAPK pathway and NFκB translocation.