• Applied Biological Chemistry
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• v.44 no.3
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• pp.137-142
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• 2001

In order to prove physiological function of biopolymer from Bacillus coagulans CE-74, in vitro experiments simulating the passive membrane transport of gastrointestinal tract were carried out using dialysis membrane. And inhibition effect of isolated biopolymer on tyrosinase and angiotensin converting enzyme (ACE) were observed. The glucose retardation index after 30 min dialysis was 43.5% in the presence of 2% biopolymer. As the dialysis period became longer, the retarding effect toward glucose absorption decreased and the effect was close to zero after 5 hr dialysis. The bile acid retardation index after 30 min dialysis was 34% and 44.2% in the presence of 1% and 2% biopolymer, respectively. The effect decreased as the dialysis time elapsed. It was measured by arosinase inhibition activity of biopolymer that inhibition effect was 48.5% in $20\;{\mu}g/{\mu}l$. In a ACE inhibition activity, biopolymer showed inhibition activity as 97% in $10\;{\mu}g/{\mu}l$.

• Korean Journal of Food Science and Technology
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• v.26 no.4
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• pp.348-354
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• 1994

Chitin and chitosan samples prepared from crab's shells under different conditions were compared for their physicochemical properties; and functionality in gastrointestinal tract by in vitro test. Their bulk density was in the range of $127{\sim}208\;mg/ml$, and their viscosity was $80{\sim}581\;cP$ in 0.1 chitin and $80{\sim}3,670\;cP$ in 0.5% chitosan solution, showing a wide variation. The degree of deacetylation in chitosan samples as determined by IR spectral analysis was relatively high, showing $81{\sim}93%$. At the same alkali concentration and reaction temperature, a longer reaction period gave an increased degree of deacetylation and lower viscosity. The water holding capacity of chitic substance became greater at higher soaking temperature; chitosan D at $37^{\circ}C$ showed the greatest value. Chitic substance with lower bulk density showed the higher water holding capacity. The retardation effect toward glucose absorption was higher in critic substances of lower density and higher water holding capacity; chitosan D showed the highest value of 38%. The retardation index toward bile acid absorption after 1 hour dialysis was $15{\sim}34%$ in chitic substances, 39% in pectin and 9% in cellulose. The retarding effect showed the highest value of 34% in chitosan D at 3% concentration.