• Applied Microscopy
• /
• v.36 no.spc1
• /
• pp.63-69
• /
• 2006

By utilizing a field emission gun and a biprism installed on a transmission electron microscope (TEM), electron holography is extensively carried out to visualize the electric and magnetic fields of nanomaterials. In the electric field analysis, the distribution of electric potential in a sharp tip made of W coated with $ZrO_2$ is visualized by applying the voltage to the tip. Denser contour lines due to the electric potential are observed with an increase in the bias voltage. In the magnetic field analysis by producing the strong magnetic field with a sharp magnetic needle made of a permanent magnet, the in situ experiment is carried out to investigate the magnetization of hard magnetic materials. The results of these experiments clearly demonstrate that electron holography is a promising advanced transmission electron microscopy technique to characterize the electric and magnetic properties of nanomaterials.

• Bulletin of the Korean Chemical Society
• /
• v.33 no.3
• /
• pp.958-962
• /
• 2012

In single-molecule fluorescence experiment, the oxygen scavenging system is indispensable for avoiding photo-bleaching of fluorescent dyes. Here we report that the gloxy-based oxygen scavenging system commonly used in single molecule fluorescence experiments can disturb the solution pH considerably. To track in situ pH change, we utilized the pH-sensitive conformational transition of i-motif and examined the transition with ensemble and single-molecule FRET measurements. Based on our results, we also suggested several practical remedies for the stability of the solution pH.

• Journal of Microbiology and Biotechnology
• /
• v.13 no.5
• /
• pp.641-646
• /
• 2003

An accurate and efficient method for measuring the mass of hairy roots using conductometry is established. A conductivity equation expressed in terms of the concentration of the ion species in the medium is suggested. By using this equation, the effect of the individual ions on the total conductivity can be quantitatively analyzed. An equation for the in situ estimation of the cell growth coefficient for determining the mass of hairy roots is established based on measurements of the nitrogen concentration and conductivity during cultivation. The proposed equation does not require preliminary experiments to determine the cell growth coefficient. Instead, the physiological characteristics of the plant species are reflected by introducing the cellular nitrogen content. Since the cell growth coefficient is determined by measuring the major ionic nutrient concentrations, it is more effective to express the dynamics of an actual culture system. This improved method for determining the mass of hairy roots was successfully utilized in a fed-batch culture system.

• 한국지구물리탐사학회:학술대회논문집
• /
• 2003.11a
• /
• pp.703-710
• /
• 2003

In the sub-surface environments, detection of the movement of contaminant substances and recharge of groundwater by rainfall are very important factors which contain porosity and effective porosity of porous media. In this paper, the applicability of permittivity methods and proposed dielectric mixing models (DDMs) are discussed. This study showed that the ratio of effective porosity to porosity of Toyoura and River sands were 0.856 and 0.843. From the relationships between the relative porosity and effective porosity, all measured values can be confirmed to outside the range to about 0.800 for Toyoura and River sands under all experiments by FDR and FDR-V systems. In the study, this permittivity equipment can be considered to be good enough to measure determining the physical parameters of saturated soils. Consequently, this permittivity method can be contributed to estimate a porosity and effective porosity of saturated porous media because it is easy and instantaneous than previous in-situ methods.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2000.05a
• /
• pp.595-598
• /
• 2000

This paper presents new cutting force measuring system for milling process. Usually, tool dynamometer is the most appropriate measuring tool in an analysis of cutting mechanism. High price and limited space, however, make it difficult to be in-situ system for controllable milling process. Although an alternative using AC current of servomotor has been suggested, it is unsuitable for cutting force control because of low bandwidth and noise. We suggest new cutting force measuring system, using two load cell placed between moving table and nut of ballscrew, and modelled on the system statically and dynamically. And to verify the accuracy of the proposed system, a series of carefully conducted experiments were carried out. Experiment results show that models are in reasonably good agreement with the experiment data.

• Proceedings of the Korean Powder Metallurgy Institute Conference
• /
• 2006.09a
• /
• pp.309-310
• /
• 2006

Porous titanium implants can be produced by powder metallurgy in combination with suitable space holder materials. Various mechanical experiments were done to characterize this material regarding the influence of the processing parameters on microstructure and mechanical properties taking into account the properties of the human bone. In this paper, the anistropic behaviour of uniaxially compacted samples was analysed in compression tests and compared to the behaviour of isostatically pressed samples. The failure of the struts of the porous titanium and the crack- initiation and -growth was examined by in-situ SEM analysis.

• Bulletin of the Korean Chemical Society
• /
• v.16 no.6
• /
• pp.507-511
• /
• 1995

Catalytic chlorination of chlorobenzene was studied in vapor phase using various solid-acid catalysts such as silica-alumina, alumina, zeolite and a modified clay prepared by impregnating bentonite with ferric chloride. The conversions of both chlorine gas and chlorobenzene showed high over silica-alumina, alumina and modified clay catalysts. However relatively large amounts of polychlorinated benzene derivatives were also observed. The active species of catalytic activity in chlorination of chlorobenzene in vapor phase were proved to be as Lewis acid sites by in-situ IR experiments. The strength of Lewis acid sites which were effective for the vapor-phase chlorination seemed to be having Hammett acidity Ho >-3.0. The selectivity to dichlorobenzenes was proved to be high over the zeolite catalyst due to their shape-selective properties. p-Dichlorobenzene or dichlorobenzene selectivities were improved more or less by changing the reaction conditions.

• Bulletin of the Korean Chemical Society
• /
• v.13 no.6
• /
• pp.680-683
• /
• 1992

The electrochemistry of benzidine and hydrazobenzene was studied in water-acetonitrile mixed solutions at various pHs and the results are reported. The cyclic voltammetric peak for the oxidation of benzidine shows a pH dependency of -62 mV/pH in the pH range of 0-3.5, no pH dependency between pH values of 3.5 and about 10.5, and of about -50 mV/pH between pH=10.50 and 14.0, indicating that oxidation mechanisms differ depending on the pH of the medium. However, the CV peak for the hydrazobenzene oxidation is shown to be independent of pH of the medium, suggesting that the proton is not involved in the rate limiting step of the electrochemical oxidation of hydrazobenzene to azobenzene. Results of in situ spectroelectrochemical experiments indicate that the oxidation products obtained during the oxidation of benzidine and hydrazobenzene depend on the result of dynamic equilibria taking place at various pHs.

• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.10
• /
• pp.1358-1364
• /
• 2011

The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculate. RT-PCR experiments using RNA extracted from bovine spermatozoa with SRY-specific primers yielded a 456 bp product, but the amount of SRY mRNA in sperm was lower than that in the testes (p<0.01). A protein of approximately 27 KDa was detected by western blotting. The SRY transcript was detected in the midpiece of approximately half the spermatozoa by in situ hybridization, and the SRY protein was detected in the heads of half the spermatozoa by immunofluorescence, indicating that SRY mRNA and protein may only be present in Y-bearing spermatozoa. These results suggest that the SRY transcript and protein are present in bovine ejaculated Y-sperm. The roles of the SRY gene in spermatogenesis, sperm motility, and the sperm-oocyte interaction merit further investigation.

• Proceedings of the KSME Conference
• /
• 2007.05a
• /
• pp.200-203
• /
• 2007

Carbon nanotubes (CNTs) have attracted an increasing attention due to their superior mechanical properties and potential application in industries. The strength of CNT has been predicted or calculated through several simulation techniques but actual experiments on stress-strain behavior are rare due to its dimensional limit, nanoscale positioning/manipulation, and instrumental resolution. We have attempted to observe straining responses of a multi-walled carbon nanotube (MWNT) with different growth methods by performing an in-situ tensile testing in a scanning electron microscope. Linear deformation and fracture behaviors of MWNT were successfully observed and its force-displacement curve was also measured from the bending stiffness and displacement of the force sensor and manipulator. We also obtained different tensile load of carbon nanotube with different growth methods.