• Archives of Plastic Surgery
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• v.41 no.6
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• pp.654-660
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• 2014

Background Reactive oxygen species (ROS) damages cell molecules, and modifies cell signaling. The nuclear factor E2-related factor (Nrf2) is a critical transcription regulator, which protects cells against oxidative damage. Nrf2 expression is increased in a large number of cancers. However, little information has been reported regarding the expression of Nrf2 in skin cancers. Hence, we explored the expression of Nrf2 protein in skin cancers. Methods The Nrf2 protein expression in 24 specimens, including 6 malignant melanomas (MM), 6 squamous cell carcinomas (SCC), 6 basal cell carcinomas (BCC), and 6 normal skin tissues, was evaluated by western blotting. Immunohistochemical staining was performed. The expression of Kelch-like ECH-associated protein 1 (Keap1), the key regulator of Nrf2, was also analyzed by western blotting. Results Small interfering RNA transfection to the melanoma cell line G361 confirmed that an approximately 66 kDa band was the true Nrf2 band. The western blot revealed that the Nrf2 protein was definitely expressed in normal skin tissues, but the Nrf2 expression was decreased in MM, SCC, and BCC. Immunohistochemical examination showed that expression of Nrf2 was decreased in all skin cancer tissues compared to the normal skin tissues. Keap1 was not expressed in all malignant skin tumors and normal skin tissues by western blot. Conclusions ROS was increased in various types of cancers which proteins were highly expressed or underexpressed. This study demonstrated that the expression of Nrf2 protein was down-regulated in human malignant skin tumors. We suggest that decreased expression of Nrf2 is related to skin cancers.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.25 no.5
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• pp.526-531
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• 2014

Vehicular communications have been receiving much attention in intelligent transport systems(ITS) by combining communication technology with automobile industries. In general, vehicular communication can be used for vehicle-to-vehicle(V2V) and vehicle-to-infrastructure( V2I) communication by adopting IEEE802.11p/1609 standard which is commonly known as wireless access in vehicular environment(WAVE). WAVE system transmits signal in 5.835~5.925 GHz frequency band with orthogonal frequency division multiplexing(OFDM) signaling. In this paper, after 32 bit processed the channel monitoring in MAC(Media Access Control) layer of WAVE system implemented according to IEEE 802.11p standard, data were received and we evaluated the performance, we built the test bed consisting of OBU(On Board Unit) in the real expressway. We transmitted WSM(WAVE Short Message) and received WSM between OBU wirelessly. And then, we calculated channel occupancy time per one frame and throughput, and evaluated the performance.

• Journal of Communications and Networks
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• v.2 no.1
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• pp.5-17
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• 2000

cdma2000 offers several enhancement as compared to TIA/EIA-95, although it remains fully compatible with TIA/EIA-95 systems and allows for a smooth migration from one to the other-Major new capability include:1)connectivity to GSM-MAP in addition to IP and IS-41 networks; 2) new layering with new LAC and MAC architectures for improved service multiplexing and QoS management and efficient use of radio resource ;3) new bands and band widths of operation in support of various operator need and constraints, as well as desire for a smooth and progressive migration to cdma 2000; and 4) flexible channel structure in support of multiple services with various QoS and variable transmission rates at up to 1 Mbps per channel and 2 Mbps per user. Given the phenomenal success of wireless services and desire for higher rate wireless services. improved spectrum efficiency was a major design goal in the elaboration of cdma2000. Major capacity enhancing features include; 1) turbo coding for data transmission: 2)fast forward link power control :3) forward link transmit diversity; 4) support of directive antenna transmission techniques; 5) coherent reverse link structure; and 6) enhanced access channel operation. As users increasingly rely on their cell phone at work and at home for voice and data exchange, the stand-by time and operation-time are essential parameters that can influence customer's satisfaction and service utilization. Another major goal of cdma2000 was therefore to enable manufacturers to further optimize power utilization in the terminal. Major battery life enhancing features include; 1) improved reverse link performance (i.e., reduced transmit power per information bit; 2) new common channel structure and operation ;3) quick paging channel operation; 4) reverse link gated transmission ; and 5) new MAC stated for efficient and ubiquitous idle time idle time operation. this article provides additional details on those enhancements. The intent is not to duplicate the detailed cdma2000 radio access network specification, but rather to provide some background on the new features of cdma2000 and on the qualitative improvements as compared to the TIA/EIA-95 based systems. The article is focused on the physical layer structure and associated procedures. It therefore does not cover the MAC, LAC, radio resource management [1], or any other signaling protocols in any detail. We assume some familiarity with the basic CDMA concepts used in TIA/EIA-95.

• Journal of Internet Computing and Services
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• v.23 no.3
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• pp.13-20
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• 2022

IEEE 802.11ay Wi-Fi is the next generation wireless technology and operates in mmWave band. It supports the MU-MIMO (Multiple User Multiple Input Multiple Output) transmission in which an AP (Access Point) can transmit multiple data streams simultaneously to multiple STAs (Stations). To this end, the AP should perform MU-MIMO beamforming training with the STAs. For efficient MU-MIMO beamforming training, it is important for the AP to estimate signal strength measured at each STA at which multiple beams are used simultaneously. Therefore, in the paper, we propose a deep learning-based link quality estimation scheme. Our proposed scheme estimates the signal strength with high accuracy by utilizing a deep learning model pre-trained for a certain indoor or outdoor propagation scenario. Specifically, to estimate the signal strength of the multiple concurrent beams, our scheme uses the signal strengths of the respective single beams, which can be obtained without additional signaling overhead, as the input of the deep learning model. For performance evaluation, we utilized a Q-D (Quasi-Deterministic) Channel Realization open source software and extensive channel measurement campaigns were conducted with NIST (National Institute of Standards and Technology) to implement the millimeter wave (mmWave) channel. Our simulation results demonstrate that our proposed scheme outperforms comparison schemes in terms of the accuracy of the signal strength estimation.

• Korean Journal of Veterinary Service
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• v.28 no.3
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• pp.259-265
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• 2005

The melanocortin 1 receptor (MC1R) encoded by the coat color extension gene (E) plays a key role in the signaling pathway of melanin synthesis. The primers for the amplification of bovine MC1R gene were designed based on a bovine MC1R gene sequence (GenBank accession no. Y19103). A size of 483bp (482bp for Hanwoo) was amplified by PCR, digested with Hpa II restriction enzyme and electrophoresed in $1.5\%$ agarose gel. When the amplified DNA product (483 bp) was digested with Hpa II restriction enzyme, Hanwoo meat showed a single band of 482bp, whereas two fragments of 325bp and 158bp were detected in Holstein, Angus and meat of Hanwoo / Holstein cross cow having back coat color phenotype, respectively. The results of this experiment Indicate that new designed primers of bovine MCIR gene may be useful for identification of Hanwoo meat from Holstein, Black Angus and Hanwoo / Holstein cross cow meat.

• Journal of the korean academy of Pediatric Dentistry
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• v.29 no.2
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• pp.243-254
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• 2002

For the purpose of evaluating the biocompatability of 3 kinds of metallic materials frequently used in pediatric dentistry (stainless steel crown, orthodontic band, orthodontic wire), cellular and molecular studies, including cell growth and proliferation, screening of cell death with determination of types whether necrosis or apoptosis and changes in expressions of related signaling molecules were examined, using cultured human gingival fibroblasts (HGF-1), HGF-1 was cultured in Dulbecco's modified Eagle's medium. among which the 3rd to 6th generations of HGF-1 were used. The specimen were divided into stainless steel crown (R), band (B) and wire (W). The immunocytochemical study was done for the detection of anti-PCNA (proliferating cell nuclear antigen) labeling. With extracted protein, western blot was done for the detection of ERK1/2, JNK, and p38, using individual antibodies. Cultured cells proliferated, remarkably till 7 day and slightly at 11 day. There was no statistical significance in the counts of proliferating HGF-1 between control and experimental groups (p>0.05). Relative growth rates were no statistically significant difference between control and experimental groups (p>0.05). PCNA labeling indexes showing similar patterns in control and experimental groups. The expressions of ERK1 and ERK2, p38 were similar in control and experimental groups. The expression of JNK increased at 1st day, slightly decreased at 4th day and markedly increased at 7th and 11 day. Although the patterns of control and experimental groups were similar, the increased expressions of JNK at late period suggest a possible stress due to inhibited cell growth and proliferation, and worse culture condition. Conclusively, the 3 kinds of metal specimens used in this study did not induce cellular and molecular hazards during short term culture of HGF-1. But, for the better clinical stability, the establishment of long period culture and animal experiment was thought necessary.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.11
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• pp.1619-1629
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• 2016

We study an efficient transmission protocol for the cellular-aided device-to-device communication model. In particular, two source-destination pairs communicate with the help of unlink and downlink cellular links. For the proposed scheme, two transmitters send their messages and the base station and two receivers receive at the first phase. Then, at the second phase, the base station sends the XOR of the messages to two receivers and they try to decode their own messages from the received signals after the first and second phases. We analyze the outage-based throughput achievable by the proposed scheme and demonstrate by simulations that the proposed scheme provides an improved outage performance compared to the conventional device-to-device communication schemes.

• The KIPS Transactions:PartC
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• v.14C no.6
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• pp.493-502
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• 2007

In this paper, we propose a handoff-aware DiffServ scheduler which intends to guarantee various QoS requirements of multimedia services for mobile nodes in TDD/CDMA based wireless networks. TDD is widely used duplexing mechanism in wireless communications. Unlike FDD, TDD allows a node to symmetrically communicate with a base station by using a single frequency band, resulting in high utilization of wireless resources. DiffServ is regarded as a relatively simple QoS support mechanism and thus it is easy to be extended. This is because DiffServ is not a per-flow based mechanisms and it does not require any signaling protocol. However, previously proposed DiffServ schedulers for wired networks can not be deployed directly into wireless networks since they do not consider properties of wireless networks. As a solution to the problem, DSS(DiffServ Supporting Scheduler) was proposed. DSS uses uplink channel, which is originally used for a node to require a base station to transmit packets, to support QoS efficiently. However, QoS does not consider handoff so that it can not support QoS for moving nodes from one cell to the other cell. Therefor. the proposed handoff support QoS mechanism is necessary for TCC/CDMA networks. The proposed scheme allows a mobile node to achieve seamless service without QoS degradation even for the handoff duration.

• Tuberculosis and Respiratory Diseases
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• v.47 no.3
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• pp.347-355
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• 1999

Background: Phospholipase C(PLC) plays a central role in cellular signal transduction and is important in cellular growth, differentiation and transformation. There are currently ten known mammalian isozymes of PLC reported to this date. Hydrolysis of phosphatidylinositol 4,5-bisphosphate($PIP_2$) by PLC produces two important second messengers, inositol 1,4,5-trisphosphate($IP_3$) and diacylglycerol. PLC-${\gamma}1$, previously, was known to be activated mainly through growth factor receptor tyrosine kinase. Other mechanisms of activating PLC-yl have been reported such as activation through tau protein in the presence of arachidonic acid in bovine brain and activation by $IP_3$, phosphatidic acid, etc. Very recently, another PLC-${\gamma}1$ activator protein such as tau has been found in bovine lung tissue, which now is considered to be AHNAK protein. But there has been no report concerning AHNAK and its associated disease to this date. In this study, we examined the expression of the PLC-${\gamma}1$ activator, AHNAK, in lung cancer specimens and their paired normal. Methods: From surgically resected human lung cancer tissues taken from twenty-eight patients and their paired normal counterparts, we evaluated expression level of AHNAK protein using immunoblot analysis of total tissue extract Immunohistochemical stain was performed with primary antibody against AHNAK protein. Results: Twenty-two among twenty-eight lung cancer tissues showed overexpression of AHNAK protein (eight of fourteen squamous cell lung cancers, all of fourteen adenocarcinomas). The resulting bands were multiple ranging from 70 to 200 kDa in molecular weight and each band was indistinct and formed a smear, reflecting mobility shift mainly due to proteolysis during extraction process. On immunohistochemistry, lung cancer tissues showed a very heavy, dense staining with anti-AHNAK protein antibody as compared to the surrounding normal lung tissue, coresponding well with the results of the western blot Conclusion: The overexpression of PLC-${\gamma}1$ activator protein, AHNAK in lung cancer may provide evidence that the AHNAK protein and PLC-${\gamma}1$ act in concerted manner in carcinogenesis.