• 한국초지조사료학회지
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• 제34권4호
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• pp.277-282
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• 2014

Nutritive value analysis of feed is very important for the growth of livestock, and ensures the efficiency of feeds as well as economic status. However, general laboratory analyses require considerable time and high cost. Near-infrared reflectance spectroscopy (NIRS) is a spectroscopic technique used to analyze the nutritive values of seeds. It is very effective and less costly than the conventional method. The sample used in this study was a corn kernel and the partial least square regression method was used for evaluating nutrient composition, digestibility, and energy value based on the calibration equation. The evaluation methods employed were the coefficient of determination ($R^2$) and the root mean squared error of prediction (RMSEP). The results showed the moisture content ($R^2_{val}=0.97$, RMSEP=0.109), crude protein content ($R^2_{val}=0.94$, RMSEP=0.212), neutral detergent fiber content ($R^2_{val}=0.96$, RMSEP=0.763), acid detergent fiber content ($R^2_{val}=0.96$, RMSEP=0.142), gross energy ($R^2_{val}=0.82$, RMSEP=23.249), in vitro dry matter digestibility ($R^2_{val}=0.68$, RMSEP=1.69), and metabolizable energy (approximately $R^2_{val}$ >0.80). This study confirmed that the nutritive components of corn kernels can be predicted using near-infrared reflectance spectroscopy.

• Asian-Australasian Journal of Animal Sciences
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• 제28권4호
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• pp.485-493
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• 2015

This study evaluated the in situ ruminal degradability, and subsequent small intestinal digestibility (SID) of dry matter, crude protein (CP), and amino acids (AA) of cottonseed meal (CSM), sunflower seed meal (SFSM) and distillers dried grains with solubles (DDGS) by using the modified three-step in vitro procedure. The ruminal degradability and subsequent SID of AA in rumen-undegradable protein (RUP-AA) varied among three protein supplements. The result show that the effective degradability of DM for SFSM, CSM, and DDGS was 60.8%, 56.4%, and 41.0% and their ruminal fermentable organic matter was 60.0%, 55.9%, and 39.9%, respectively. The ruminal degradable protein (RDP) content in CP for SFSM, CSM, and DDGS was 68.3%, 39.0%, and 32.9%, respectively, at the ruminal solid passage rate of 1.84%/h. The SFSM is a good source of RDP for rumen micro-organisms; however, the SID of RUP of SFSM was lower. The DDGS and CSM are good sources of RUP for lambs to digest in the small intestine to complement ruminal microbial AA of growing lambs. Individual RUP-AA from each protein source was selectively removed by the rumen microorganisms, especially for Trp, Arg, His, and Lys (p<0.01). The SID of individual RUP-AA was different within specific RUP origin (p<0.01). Limiting amino acid was Leu for RUP of CSM and Lys for both RUP of SFSM and DDGS, respectively. Therefore, different protein supplements with specific limitations should be selected and combined carefully in growing lambs ration to optimize AA balance.

• Asian-Australasian Journal of Animal Sciences
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• 제2권2호
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• pp.123-128
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• 1989

An experiment was conducted to study the effect of frequency of defoliation on the ensiling characteristics and nutritive value of Guineaecotype 'A' (Panicum maximum Jacq) grass. Guinea grass harvested at 2, 3, 4, 5, 6, 7, 8, 9, 10 and 12-week cutting intervals was chopped (2 cm) and ensiled alone or with the addition of coconut (Cocos nucifera L.) meal (w/w-fresh weight basis), for periods of 6, 7 or 8 weeks in 2-litre laboratory silos. Dry matter, water soluble carbohydrates, lactic acid, pH, ash, nitrogen and digestibility in vitro were measured in representative samples of preensiled and ensiled material. Dry matter content of silages prepared from herbage harvested at 2 and 3 weeks intervals was lower (P<0.05) compared to longer defoliation intervals. The pH of silage prepared from herbage over 8 weeks old were higher (P<0.05) than those harvested below 8 weeks. The water soluble carbohydrate content of silage prepared from 3-weekly defoliated herbage was higher (P<0.05) than those prepared from longer periods. Lengthened growth period decreased (P<0.001) the crude protein content of the ensiled material, whereas the addition of coconut meal resulted in marginal increases. In vitro organic matter digestibility of the silages decreased (P<0.05) with the increase in forage maturity.

• 한국식품영양과학회지
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• 제16권2호
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• pp.98-104
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• 1987

메뚜기 건조시료의 in vitro 소화율과 trypsin 비소화성물질을 정량하고, 아미노산 분석결과를 이용하여 이들 시료의 품질을 C-PER technique으로 평가하였다. 메뚜기 건조시료의 조단백질이 73% 이상(건물중량)으로 단백질원으로서의 가치가 인정되며 chitin질을 제거하면 순도가 높은 단백질을 얻을 수 있을 것이다. 특히 인(p)과 아연(zn)의 함량이 높게 나타났다. In vitro 소화율은 탈지시료가 일건 및 열풍건조 시료에 비하여 높게 나타나 열처리로 인한 소화율의 감소를 가져왔으며 이는 trypsin 비소화성물질의 증가와 밀접한 관계를 가진다. 아미노산 분석결과 lysine의 함량이 ANRC casein에 비하여 특히 낮았으며 tryptophan과 methionine의 함량이 낮았고 aspartic acid, glutamic acid, arginine등의 함량이 일건 및 열풍건조 시료에서 증가하는 경향을 나타내었다. 전반적인 단백효율비의 계산 결과 C-PER이 아미노산 분석결과로만 계산되는 DC-PER에 비해 높은 결과를 나타내었고, 예측소화율은 90%정도로 나타났다. 이상에서 메뚜기 일건 및 열건시료는 산화지질과의 반응으로 단백질의 손실을 초래하였고 TIS의 증가, 아미노산 조성의 변화로 in vitro 소화율저하를 초래하였다.

• 한국작물학회지
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• 제48권3호
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• pp.276-280
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• 2003

남부지역에 적응한 사초용 유채의 춘파 품종을 선발하기 위하여 3월 25일에 파종한 그 결과는 다음과 같다. 1. 다수성이며 조단백질 함량과 in vitro dry matter digestibility(IVDMD)가 높고 NDF, ADF, cellulose 및 lignin 함량이 낮아서 영양가가 우수한 춘파 용 사초 유채는 Velox 품종이었다. 2. Velox품종은 초장이 길고 경태가 두꺼웠으며 주경의 잎도 많았고 생체중 과 총건물수량이 가장 많았다. 3. 모든 형질에 대한 유전력은 대부분의 형질에서 높았으나 hemicellulose에서만이 낮게 평가되었다.

• 한국초지조사료학회지
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• 제24권1호
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• pp.81-90
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• 2004

Farmers need timely information on the nutritional status of their animals and the nutritive value of pastures and supplementary feeds if they are to apply successfully this existing nutritional information. Near infrared reflectance(NIR) spectroscopy has been used over the last forty years to analyse accurately protein, fiber, and other organic components in animal foods. NIR spectroscopy is a rapid, non-destructive, and non-polluting technology. When properly calibrated, NIR spectroscopy is used successfully with both concentrate and forage feeds. NIR methods predict in vitro digestibility accurately and precisely, and can predict in vivo digestibility at least as well as conventional "wet chemistry" methods such as in vivo digestion or the pepsin-cellulase method, and much more rapidly. NIR technology has been applied to the routine monitoring (through analysis of feces samples) of the nutritional status of cattle and other grazing animals. This report reviews the use of near infrared reflectance(NIR) spectroscopy to monitor the nutritive value of animal feeds and the nutritional status of grazing animals.

• Asian-Australasian Journal of Animal Sciences
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• 제33권3호
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• pp.446-455
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• 2020

Objective: Our recent series of laboratory- and large-scale experiments confirmed that under aerobic and anaerobic conditions, sodium metabisulfite (SMB) was effective in preserving nutrients and antioxidant capacity of highly perishable fruit and vegetable discards (FVD). Hence, the purpose of this study was to examine how partial inclusion of SMB-treated FVD in total mixed ration (TMR) influences in vitro ruminal fermentation, whole-tract digestibility, nitrogen metabolism, blood metabolites, and voluntary feed intake of sheep. Methods: The FVD were mixed thoroughly with 6 g SMB/kg wet biomass and kept outdoors under aerobic conditions for 7 days. Four TMRs including four levels of SMB-treated FVD (as-fed basis) at 0%, 10%, 20%, and 30% (equaling to 0%, 1.9%, 3.8%, and 5.7% on dry matter basis, respectively), were prepared as replacement for corn grain. The ruminal fermentation metabolites were studied using an in vitro gas production test. Four mature male Corriedale sheep were assigned at random to the 4 diets for two separate sub-experiments; i) digestibility trial with four 21-d periods, and ii) voluntary feed intake trial with four 28-d periods. Results: Inclusion of SMB-treated FVD in the TMR tended to quadratically increase partitioning factor. No effect was seen on total-tract digestibility of organic matter, ether extract, crude protein, and acid detergent fiber, except for neutral detergent fiber digestibility that tended to linearly increase with increasing SMB-treated FVD in the TMR. The progressive increase of FVD preserved with SMB in the diet had no effect on nitrogen metabolism. Treatment had no effect on serum antioxidant capacity and blood metabolites assayed. Voluntary feed intake was not impaired by inclusion of SMB-treated FVD in the TMR. Conclusion: It appears that FVD preserved with SMB can be safely incorporated into TMR as replacement of corn grain without impairment of nutrient metabolism and feed intake.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.620-633
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• 1998

Methods developed for measuring digestibility and availability of amino acids in feedstuffs used in pig nutrition are reviewed. Digestibility is a proportion of an amino acid in a feed that is absorbed from the digestive tract and should be determined from the difference between the amount of amino acid consumed and passing the distal ileum. Techniques for ileal digesta sampling including various types of cannulas: a re-entrant, T-piece, IPV, IPVC and ileaorectal anastomosis are described and comparisons amongst these methods are presented. Other methodologies like mobile bag technique, in vitro assays and mathematical prediction method are also described. Significance and methodologies for measurement of endogenous nitrogen and amino acids losses at the distal ileum and their effect on the apparent and true nitrogen and amino acid digestibilities in feeds are discussed. Factors influencing the apparent and true amino acid digestibilities such as dry matter intake, protein, fibre and antinutritive compounds content in the diet are discussed. Amino acid bioavailability -the proportion of the total amino acid digested and absorbed in a form utilized in metabolism - measured by the growth assay may differ from its ileal digestibility. Chemical methods for determination of available lysine content in heat treated feeds are evaluated.

• 한국식품과학회지
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• 제21권3호
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• pp.441-445
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• 1989

인삼엽 단백질의 식량화를 위하여 엽단백질 농축물(LPC)을 조제하여 그 아미노산 조성과 인공소화율을 검토하였다. 인삼엽을 $-20^{\circ}C$ 냉아세톤으로 처리하고 0.2% NaOH(0.5% SDS, 0.5% 2-ME 함유)로 단백질을 추출하며 인삼 LPC를 얻었다. 인삼 LPC의 일반성분으로는 지방과 회분이 1% 미만이었고 단백질은 75% 내외로 많았으며 총 당은 약 5% 이었다. 총 사포닌 함량은 약 1.2%이고 panaxadiol과 panaxatriol의 비는 1.67이었다. 아미노산 조성은 aspartic acid의 함량이 약 13.4%로 가장 많았으며 methionine의 함량은 매우 낮았다. 필수아미노산토 고루 들어 있었고 총 아미노산에 대한 필수아미노산은 54.5%이였고 E/T ratio는 3.02이었다. LPC의 제 1제한아미노산은 함황아미노산이고, 그 amino acid score는 43.1이었다. Pepsin및 trypsin을 사용한 인삼 LPC의 소화율은 비교단백질인 milk casein에 비하며 낮았다.

• Asian-Australasian Journal of Animal Sciences
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• 제33권2호
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• pp.230-235
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• 2020

Objective: This study was realized to evaluate the nutritional value of rice grains as a replacement for corn grains in the diet of growing Hanwoo steers. Methods: Two experimental diets were prepared: i) Corn total mixed ration (TMR) consisting of 20% corn grains and ii) Rice TMR consisting of 20% rice grains, in a dry matter (DM) basis. These treatments were used for in vitro rumen fermentation and in vivo growth trials. In the rumen fermentation experiment, the in vitro DM digestibility (IVDMD), in vitro crude protein digestibility (IVCPD), in vitro neutral detergent fiber digestibility, pH, ammonia nitrogen, and volatile fatty acids (VFA) were estimated at 48 h, and the gas production was measured at 3, 6, 12, 24, and 48 h. Twenty four growing Hanwoo steers (9 months old; body weight [BW]: 259±13 kg) were randomly divided into two treatment groups and the BW, dry matter intake (DMI), average daily gain (ADG), and feed conversion ratio (FCR) were measured. Results: The in vitro experiment showed that the IVDMD, IVCPD, and VFA production of the Rice TMR were higher than those of the Corn TMR (p<0.05). The growth trial showed no differences (p>0.05) in the final BW, ADG, DMI, and FCR between the two TMRs. Conclusion: The use of rice grains instead of corn grains did not exhibit any negative effects on the rumen fermentation or growth performance, thereby rice grains with a DM of less than 20% could be used as a starch source in the diet of growing steers.